RÉSUMÉ
microRNAs, a family of small non-coding RNAs, involve in the pathogenesis of several types of cancers, including laryngeal squamous cell carcinoma (LSCC). MiR-370 is frequently aberrant expressed in various types of human cancer including LSCC. However, the role for miR-370 in LSCC remains elusive. Here, we demonstrate that miR-370 was down-regulated in human LSCC tissues. Furthermore, there was an inverse relationship between Forkhead Box ml (FoxM1), which was up-regulated and miR-370 expression in LSCC tissues. FoxM1 was subsequently predicted by bioinformatics and verified to be a target of miR-370 by Luciferase reporter assay. Restored expression of miR-370 in Hep2 cells significantly inhibited cell proliferation. In conclusion, our results suggest that miR-370 may function as a tumor suppressor in LSCC through downregulation of FoxM1, suggesting that miR-370 could serve as a novel potential maker for LSCC therapy.
Sujet(s)
Carcinome épidermoïde/génétique , Facteurs de transcription Forkhead/génétique , Gènes suppresseurs de tumeur , Tumeurs du larynx/génétique , microARN/génétique , Séquence nucléotidique , Sites de fixation , Carcinome épidermoïde/métabolisme , Carcinome épidermoïde/anatomopathologie , Techniques de culture cellulaire , Lignée cellulaire tumorale , Prolifération cellulaire , Régulation négative , Protéine M1 à motif en tête de fourche , Gènes rapporteurs , Humains , Tumeurs du larynx/métabolisme , Tumeurs du larynx/anatomopathologie , Luciférases de Renilla/génétique , Données de séquences moléculaires , Plasmides , Transfection , Régulation positiveRÉSUMÉ
OBJECTIVE: To explore and evaluate the application of epiglottis with sternohyoid muscle in the surgery for laryngeal cancer. METHODS: Two fifty patients with laryngeal cancer were treated by partial laryngectomy and the laryngeal defects were reconstructed by epiglottic flap and sternohyoid muscle fascia flap. The staging of tumors: T2N0M0 23 cases, T3N1M0 14 cases, T3N2M0 13 cases. All of them received post radical radiotherapy with average 60 Gy. The patients were followed up for 3 to 5 years. Thirty patients underwent neck dissection. RESULTS: The three and five years survival rates were 90% and 80% respectively. The total cases eat breath pronounced well. The decannulation rate was 96%. CONCLUSION: After partial laryngectomy in laryngeal cancer epiglottic flap and sternohyoid muscle fascia flap were performed reconstruction of laryngeal function.