Advancing Cancer Treatment: Enhanced Combination Therapy through Functionalized Porous Nanoparticles.

Cancer remains a major global health challenge, necessitating the development of innovative treatment strategies. This review focuses on the functionalization of porous nanoparticles for combination therapy, a promising approach to enhance cancer treatment efficacy while mitigating the limitations associated with conventional methods. Combination therapy, integrating multiple treatment modalities such as chemotherapy, phototherapy, immunotherapy, and others, has emerged as an effective strategy to address the shortcomings of individual treatments. The unique properties of mesoporous silica nanoparticles (MSN) and other porous materials, like nanoparticles coated with mesoporous silica (NP@MS), metal-organic frameworks (MOF), mesoporous platinum nanoparticles (mesoPt), and carbon dots (CDs), are being explored for drug solubility, bioavailability, targeted delivery, and controlled drug release. Recent advancements in the functionalization of mesoporous nanoparticles with ligands, biomaterials, and polymers are reviewed here, highlighting their role in enhancing the efficacy of combination therapy. Various research has demonstrated the effectiveness of these nanoparticles in co-delivering drugs and photosensitizers, achieving targeted delivery, and responding to multiple stimuli for controlled drug release. This review introduces the synthesis and functionalization methods of these porous nanoparticles, along with their applications in combination therapy.

Role of Functionalized Peptides in Nanomedicine for Effective Cancer Therapy.

Peptide-functionalized nanomedicine, which addresses the challenges of specificity and efficacy in drug delivery, is emerging as a pivotal approach for cancer therapy. Globally, cancer remains a leading cause of mortality, and conventional treatments, such as chemotherapy, often lack precision and cause adverse effects. The integration of peptides into nanomedicine offers a promising solution for enhancing the targeting and delivery of therapeutic agents. This review focuses on the three primary applications of peptides: cancer cell-targeting ligands, building blocks for self-assembling nanostructures, and elements of stimuli-responsive systems. Nanoparticles modified with peptides improved targeting of cancer cells, minimized damage to healthy tissues, and optimized drug delivery. The versatility of self-assembled peptide structures makes them an innovative vehicle for drug delivery by leveraging their biocompatibility and diverse nanoarchitectures. In particular, the mechanism of cell death induced by self-assembled structures offers a novel approach to cancer therapy. In addition, peptides in stimuli-responsive systems enable precise drug release in response to specific conditions in the tumor microenvironment. The use of peptides in nanomedicine not only augments the efficacy and safety of cancer treatments but also suggests new research directions. In this review, we introduce systems and functionalization methods using peptides or peptide-modified nanoparticles to overcome challenges in the treatment of specific cancers, including breast cancer, lung cancer, colon cancer, prostate cancer, pancreatic cancer, liver cancer, skin cancer, glioma, osteosarcoma, and cervical cancer.

Light-Responsive Layer-By-Layer Film Containing Gold Nanorods for Sequential Drug Release.

Chemically and physically stable multidrug-loaded layer-by-layer (LbL) films are promising candidates for sequential and on-demand drug release at concentrations suitable for various applications. The synergistic effect of the sequential release of drugs may enhance their therapeutic efficacy in treating skin cancer and other complex medical conditions. In this study, we prepared LbL films by alternating the deposition of cationic linear polyethylenimine, camptothecin (CPT)-loaded gold nanorods (GNRs), anionic poly(styrenesulfonate), and doxorubicin (DOX) based on electrostatic interactions. The film exhibited loading of CPT and DOX, which could be tuned according to the requirements of the application by changing the parameters of the LbL process. Herein, CPT was encapsulated in GNRs and showed good stability and absorption in the near-infrared (NIR) range (650-900 nm). The prepared LbL film showed a pH-dependent DOX release. Subsequently, the functionalized GNRs showed excellent photothermal properties, which assisted the on-demand release of CPT upon NIR irradiation with further release of DOX. Our results suggest that the LbL approach for sequential drug release can be an effective drug delivery platform owing to its cytocompatibility, anticancer effects, and stimuli-responsive properties.

Smart Delivery Platform Using Core-Shell Nanofibers for Sequential Drug Release in Wound Healing.

An effective approach to accelerating wound healing is through a smart delivery platform that releases drugs according to the needs of different healing periods. With the growing demand for wound care and treatment, electrospun nanofibers have attracted considerable attention owing to their simple and versatile method of manufacturing, unique structure, and biological functions similar to those of the extracellular matrix. Moreover, nanofibers can be loaded with active substances that promote targeted wound healing. In this study, we investigated the performance of a core-shell nanofiber platform loaded with two drugs in the core and shell, respectively. The shell polymer, poly-l-lactic acid, initially releases the encapsulated drug into an aqueous solution at room temperature. Gold nanorods with near-infrared absorbance were incorporated in the core polymer poly(N-isopropylacrylamide) to produce localized heat by plasmon resonance when exposed to light. This allows the thermally responsive core polymer to swell and shrink for programmable drug release. Our study provides a versatile platform for controlled and safe drug delivery to wound sites and could be applied to the treatment of other topical diseases.

Microbubble Delivery Platform for Ultrasound-Mediated Therapy in Brain Cancers.

The blood-brain barrier (BBB) is one of the most selective endothelial barriers that protect the brain and maintains homeostasis in neural microenvironments. This barrier restricts the passage of molecules into the brain, except for gaseous or extremely small hydrophobic molecules. Thus, the BBB hinders the delivery of drugs with large molecular weights for the treatment of brain cancers. Various methods have been used to deliver drugs to the brain by circumventing the BBB; however, they have limitations such as drug diversity and low delivery efficiency. To overcome this challenge, microbubbles (MBs)-based drug delivery systems have garnered a lot of interest in recent years. MBs are widely used as contrast agents and are recently being researched as a vehicle for delivering drugs, proteins, and gene complexes. The MBs are 1-10 µm in size and consist of a gas core and an organic shell, which cause physical changes, such as bubble expansion, contraction, vibration, and collapse, in response to ultrasound. The physical changes in the MBs and the resulting energy lead to biological changes in the BBB and cause the drug to penetrate it, thus enhancing the therapeutic effect. Particularly, this review describes a state-of-the-art strategy for fabricating MB-based delivery platforms and their use with ultrasound in brain cancer therapy.

Effects of Nanobubbles in Dermal Delivery of Drugs and Cosmetics.

Dermal delivery, which delivers drugs and cosmetics through the skin, has attracted significant attention due to its non-invasive and simple administration compared with oral or injectable administration. However, delivery of the ingredients through the skin barrier is difficult because the primary function of the skin is to protect the human body by preventing the invasion of contaminants. Although various techniques have been developed to overcome skin barriers, chemical toxicity, complicated processes, and expensive equipment still remain as obstacles. Moreover, green chemistry, which minimizes or eliminates the use of toxic chemicals, is required in the cosmetic industry. Thus, the development of a new method for dermal delivery is required. In this study, we provide a new method for dermal delivery using nanobubbles (NBs). NBs generated in oil improve the delivery effect of the active ingredients through the high Brownian motion and charge-balancing effect. Franz cell experiments and depigmentation experiments using the B16F10 melanoma cells were conducted to confirm the enhanced delivery effects. The system using NBs will contribute to the advancement of the dermal delivery of drugs and cosmetics.

Stimuli-Responsive Adaptive Nanotoxin to Directly Penetrate the Cellular Membrane by Molecular Folding and Unfolding.

Biological nanomachines, including proteins and nucleic acids whose function is activated by conformational changes, are involved in every biological process, in which their dynamic and responsive behaviors are controlled by supramolecular recognition. The development of artificial nanomachines that mimic the biological functions for potential application as therapeutics is emerging; however, it is still limited to the lower hierarchical level of the molecular components. In this work, we report a synthetic machinery nanostructure in which actuatable molecular components are integrated into a hierarchical nanomaterial in response to external stimuli to regulate biological functions. Two nanometers core-sized gold nanoparticles are covered with ligand layers as actuatable components, whose folding/unfolding motional response to the cellular environment enables the direct penetration of the nanoparticles across the cellular membrane to disrupt intracellular organelles. Furthermore, the pH-responsive conformational movements of the molecular components can induce the apoptosis of cancer cells. This strategy based on the mechanical motion of molecular components on a hierarchical nanocluster would be useful to design biomimetic nanotoxins.

On-Demand Drug Delivery Systems Using Nanofibers.

On-demand drug-delivery systems using nanofibers are extensively applicable for customized drug release based on target location and timing to achieve the desired therapeutic effects. A nanofiber formulation is typically created for a certain medication and changing the drug may have a significant impact on the release kinetics from the same delivery system. Nanofibers have several distinguishing features and properties, including the ease with which they may be manufactured, the variety of materials appropriate for processing into fibers, a large surface area, and a complex pore structure. Nanofibers with effective drug-loading capabilities, controllable release, and high stability have gained the interest of researchers owing to their potential applications in on-demand drug delivery systems. Based on their composition and drug-release characteristics, we review the numerous types of nanofibers from the most recent accessible studies. Nanofibers are classified based on their mechanism of drug release, as well as their structure and content. To achieve controlled drug release, a suitable polymer, large surface-to-volume ratio, and high porosity of the nanofiber mesh are necessary. The properties of nanofibers for modified drug release are categorized here as protracted, stimulus-activated, and biphasic. Swellable or degradable polymers are commonly utilized to alter drug release. In addition to the polymer used, the process and ambient conditions can have considerable impacts on the release characteristics of the nanofibers. The formulation of nanofibers is highly complicated and depends on many variables; nevertheless, numerous options are available to accomplish the desired nanofiber drug-release characteristics.

Stimuli-Responsive Nanofibers Containing Gold Nanorods for On-Demand Drug Delivery Platforms.

On-demand drug delivery systems using nanofibers have attracted significant attention owing to their controllable properties for drug release through external stimuli. Near-infrared (NIR)-responsive nanofibers provide a platform where the drug release profile can be achieved by the on-demand supply of drugs at a desired dose for cancer therapy. Nanomaterials such as gold nanorods (GNRs) exhibit absorbance in the NIR range, and in response to NIR irradiation, they generate heat as a result of a plasmon resonance effect. In this study, we designed poly (N-isopropylacrylamide) (PNIPAM) composite nanofibers containing GNRs. PNIPAM is a heat-reactive polymer that provides a swelling and deswelling property to the nanofibers. Electrospun nanofibers have a large surface-area-to-volume ratio, which is used to effectively deliver large quantities of drugs. In this platform, both hydrophilic and hydrophobic drugs can be introduced and manipulated. On-demand drug delivery systems were obtained through stimuli-responsive nanofibers containing GNRs and PNIPAM. Upon NIR irradiation, the heat generated by the GNRs ensures shrinking of the nanofibers owing to the thermal response of PNIPAM, thereby resulting in a controlled drug release. The versatility of the light-responsive nanofibers as a drug delivery platform was confirmed in cell studies, indicating the advantages of the swelling and deswelling property of the nanofibers and on-off drug release behavior with good biocompatibility. In addition, the system has potential for the combination of chemotherapy with multiple drugs to enhance the effectiveness of complex cancer treatments.

Risk assessment of educational support staff's work in schools.

BACKGROUND: Educational support staff (educational staff) in schools perform widely distinctive activities according to their occupation and work process. OBJECTIVE: This study analyzes the possible incidents for each occupation and work process, and suggests a risk matrix prioritizing the risks of educational staff's work. METHODS: A total of 1,566 injuries of educational staff, registered for occupational injuries and illnesses between 2015 and 2016, were used to develop the risk matrix considering injuries, sick leave days, and disabilities. RESULTS: Among the 1,566 injuries, 'Cook-cooking/serving: Burn' (8.5%) was the most frequent incident. 'Cook-manual materials handling (MMH): musculoskeletal disorders (MSDs)' (241.6 sick leave days) was the most severe incident, and the number of disabilities was high in the forms of 'Cook-cooking/serving: MSDs.' According to risk assessment results, possible incidents predicted as 'High' level of risk management were in the forms of 'Cook-cooking/serving: MSDs,' 'Cook-MMH: Slip and trip,' 'Cook-cleaning/repairing: Fall from a height,' 'Cook-walking: Slip and trip,' 'Cook-MMH: MSDs,' 'Cook-cooking/serving: Burn,' 'Cook-cleaning/repairing: Slip and trip,' 'Custodian-cleaning/repairing: Fall from a height,' and 'Instructor-walking: Slip and trip.' CONCLUSIONS: The results can be used to develop an effective injury prevention policy, and to allocate resources for risk management in ensuring school safety.

Development of Gold Nanoparticle Micropatterns for the Electrical Detection of Proteins.

Protein analysis can be used to efficiently detect the early stages of various diseases. However, conventional protein detection platforms require expensive or complex equipment, which has been a major obstacle to their widespread application. In addition, uncertain signals from non-specific adhesion interfere with the precise interpretation of the results. To overcome these problems, the development of a technique that can detect the proteins in a simple method is needed. In this study, a platform composed of gold nanoparticles (GNPs) was fabricated through a simple imprinting method for protein detection. The corrugated surface naturally formed by the nanoparticle assemblies simultaneously increases the efficiency of adhesion and binding with analytes and reduces undesired interactions. After forming the GNP micropatterns, post-functionalization with both cationic and neutral ligands was performed on the surface to manipulate their electrostatic interaction with proteins. Upon protein binding, the change in the electrical values of the micropatterns was recorded by using a resistance meter. The resistance of the positively charged micropatterns was found to increase due to the electrostatic interaction with proteins, while no significant change in resistance was observed for the neutral micropatterns after immersion in a protein solution. Additionally, the selective adsorption of fluorescent proteins onto the micropatterns was captured using confocal microscopy. These simply imprinted GNP micropatterns are sensitive platforms that can detect various analytes by measuring the electrical resistance with portable equipment.

Drug resistance-free cytotoxic nanodrugs in composites for cancer therapy.

Drug resistance is a major cause of treatment failure for small-molecule cancer chemotherapies, despite the advances in combination therapies, drug delivery systems, epigenetic drugs, and proteolysis-targeting chimeras. Herein, we report the use of a drug resistance-free cytotoxic nanodrug as an alternative to small-molecule drugs. The present nanodrugs comprise 2 nm core gold nanoparticles (AuNPs) covered completely with multivalent hydrocarbon chains to a final diameter of ∼10 nm as single drug molecules. This hydrophobic drug-platform was delivered in composite form (∼35 nm) with block-copolymer like other small-molecular drugs. Upon uptake by cells, the nanodrugs enhanced the intracellular levels of reactive oxygen species and induced apoptosis, presumably reflecting multivalent interactions between aliphatic chains and intracellular biomolecules. No resistance to our novel nanodrug was observed following multiple treatment passages and the potential for use in cancer therapy was verified in a breast cancer patient-derived xenograft mouse model. These findings provide insight into the use of nano-scaled compounds as agents that evade drug resistance to cancer therapy.

Analysis of occupational injuries and the risk management of automobile parts manufacturing work.

Background. Unexpected occupational injuries frequently occur in the automobile parts manufacturing industry. This study investigates the characteristics of occupational injuries and risk management for the workers in the industry. Methods. From the national industrial accident compensation data in Korea, 1530 occupational injuries were analyzed by nature and source of injury and illness according to work process. Also, this study derived the risk management level for prioritizing preventative measures. Results. The most critical injuries were 'ACC (amputation or caught in or crushed or compressed)' caused by 'misoperation or malfunction of machine and equipment' in the 'fabrication' and the 'maintenance' processes. Possible incidents predicted as a 'high' level of risk management were 'struck' (struck by or against objects) caused by 'misoperation or malfunction of machine and equipment' and ACC during 'installation of jig and mold' in the 'fabrication' process. ACC during 'maintenance' of 'operating jig and mold' is also classified 'high'. Discussion and conclusion. Using the findings of this study, effective preventative measures to reduce occupational injuries according to the risk level are suggested and discussed for automobile parts manufacturing works.

Comparison of work-related traffic crashes between male taxi drivers aged ≥65 years and <65 years in South Korea.

BACKGROUND: The percentage of drivers aged ≥65 years among all Korean taxi drivers has risen sharply from 3.2% in 2006 to 22.0% in 2016. OBJECTIVE: This study compared the characteristics of work-related traffic crashes between male taxi drivers aged ≥65 years and <65 years. METHODS: Using the national compensation data of South Korea, 586 injured male taxi drivers were analyzed based on driver-related (work experience, company size, employment status, injured body part, and injury type) and crash-related factors (time and day of the crash, weather condition, road type, violation and drowsiness) by age group. RESULTS: For the injured drivers aged ≥65, percentages of the crashes related to some factors were lower than those of the drivers aged <65 years: at night (55.2% vs. 64.0%), on rainy or snowy day (7.8% vs. 21.3%), on straight road (40.5% vs 50.9%) and on the expressway (6.9% vs. 13.0%). However, the percentage of the crashes related to a violation for the injured drivers aged ≥65 years (23.3%) was higher than that of the drivers aged <65 years (13.4%). Furthermore, the taxi drivers aged ≥65 years had a higher death rate (14.7%) than the drivers aged <65 years (8.5%). CONCLUSIONS: The results can be useful for injury prevention policies and guidelines for elderly taxi drivers such as strengthened qualification tests for the aged drivers and improvement of the working environment.

Effects of age and violations on occupational accidents among motorcyclists performing food delivery.

BACKGROUND: Risk factors for motorcycle injuries are associated with rider-related factors and crash-related factors. OBJECTIVE: This study investigates the effects of age and violations on occupational accidents among motorcyclists performing food delivery. METHODS: This study analyzed 1,317 injured couriers regarding rider-related factors and crash-related factors according to rider's age or violations. RESULTS: Among injured riders, 67.4% were temporary workers, 76.1% worked in small companies with <5 employees, 58.7% in the nighttime, and 51.5% had a work experience of <1 month. However, among the injured teens, 93.5% were temporary workers, 87.0% in companies with <5 employees, 79.5% in nighttime, and 61.4% with work experience of <1 month. The proportion of novice with <1 month, of the temporary worker, of 'head/face/neck' injury, or of the 'concussion/hemorrhage' type of injury all decreased with age. However, the proportion of 'fracture,' 'rider alone,' or 'death or disability' accidents increased with age. Furthermore, the violation rate was high in teens (17.4%), at night (15.4%), or in type of 'crash with a car' (26.2%). The violation rate decreased with age. CONCLUSIONS: The results are expected to be useful for injury prevention policies and guidelines in the food delivery industries.

Effects of female worker's salary and health on safety education and compliance in three sectors of the service industry.

BACKGROUND: According to a survey of the working population of women by industry, service industries accounted for the majority. OBJECTIVE: The effects of female worker's salary and self-rated health on safety education and compliance in three sectors of the service industry are reported. METHODS: A sample of 700 women service workers were surveyed; their age, work experience, salary, self-rated health, safety educational participation, and compliance were recorded. RESULTS: The salary of female service workers was directly related to safety educational participation and compliance, as well as the health levels they reported. CONCLUSIONS: The results suggest that an increase in the self-rated health and salary of female workers can contribute to enhancing safety educational participation and compliance. Development of educational programs in prevention and safety compliance is expected to contribute to the prevention of industrial accidents in the service sector.

Carbon Dots as an Effective Fluorescent Sensing Platform for Metal Ion Detection.

Fluorescent carbon dots (CDs) including carbon quantum dots (CQDs) and graphene quantum dots (GQDs) have drawn great interest because of their low cost and low toxicity, and they represent a new class of carbon materials prepared by simple synthetic routes. In particular, the optical properties of CDs can be easily tuned by the surface passivation of the organic layer and functionalization of the CDs. Based on the advantages of these carbon materials, CQDs and GQDs have been applied in various fields as nanoplatforms for sensing, imaging, and delivery. In this review, we discuss several synthetic methods for preparing CQDs and GQDs, as well as their physical properties, and further discuss the progress in CD research with an emphasis on their application in heavy metal sensing.

Hyaluronic Acid-Coated Nanomedicine for Targeted Cancer Therapy.

Hyaluronic acid (HA) has been widely investigated in cancer therapy due to its excellent characteristics. HA, which is a linear anionic polymer, has biocompatibility, biodegradability, non-immunogenicity, non-inflammatory, and non-toxicity properties. Various HA nanomedicines (i.e., micelles, nanogels, and nanoparticles) can be prepared easily using assembly and modification of its functional groups such as carboxy, hydroxy and N-acetyl groups. Nanometer-sized HA nanomedicines can selectively deliver drugs or other molecules into tumor sites via their enhanced permeability and retention (EPR) effect. In addition, HA can interact with overexpressed receptors in cancer cells such as cluster determinant 44 (CD44) and receptor for HA-mediated motility (RHAMM) and be degraded by a family of enzymes called hyaluronidase (HAdase) to release drugs or molecules. By interaction with receptors or degradation by enzymes inside cancer cells, HA nanomedicines allow enhanced targeting cancer therapy. In this article, recent studies about HA nanomedicines in drug delivery systems, photothermal therapy, photodynamic therapy, diagnostics (because of the high biocompatibility), colloidal stability, and cancer targeting are reviewed for strategies using micelles, nanogels, and inorganic nanoparticles.

Structural equation modeling of work-related conditions on safety perception and safety education in waste and recycling collectors.

Waste and recycling collectors are responsible for cleaning, collecting, transporting and sorting various garbage. The purpose of this study is to investigate relationships between work-related conditions, safety perception and safety education in waste and recycling collectors. A total of 675 workers who collect domestic, street, recyclable or food waste were selected by stratified sampling in the waste and recycling industry. A structural equation model was used to analyze whether work-related conditions and safety perception affect safety education factors and whether there exists a causal relationship between them. According to the results of structural equation modeling, work-related conditions directly affect the level of safety education and indirectly affect the level of safety education by having an impact on safety perception. Waste and recycling collectors are more likely to participate in safety education and to be more concerned about safety education as company size and salaries increase. The results of this study suggest that accident-prevention measures that are appropriate to job characteristics are effective because worker's features and their safety climate are different according to job type in the service industry.

Gender differences in public office workers' satisfaction, subjective symptoms and musculoskeletal complaints in workplace and office environments.

This study investigates differences between male and female public office workers' satisfaction levels, sick building syndrome (SBS) symptoms and musculoskeletal disorder (MSD) complaints in workplace and office environments. Questionnaire surveys were performed in 30 offices from 15 public institutions. Male and female workers of the same age were coupled and selected from each office, gathering a total of 120 male and 120 female subjects. The results show that differences exist between genders in noise and lighting satisfaction levels, SBS-related symptoms (eye, nose, skin) and MSD complaints of hand/wrist/finger, while there is no difference in overall satisfaction level of office environments. The study also suggests that office design for public office workers should take into account gender differences in preventing MSDs and also SBS. The findings of this study are expected to serve as basic data for designing effective public office environments.