PFOA accumulation in the leaves of basil (Ocimum basilicum L.) and its effects on plant growth, oxidative status, and photosynthetic performance.

BACKGROUND: Perfluoroalkyl substances (PFASs) are emerging contaminants of increasing concern due to their presence in the environment, with potential impacts on ecosystems and human health. These substances are considered "forever chemicals" due to their recalcitrance to degradation, and their accumulation in living organisms can lead to varying levels of toxicity based on the compound and species analysed. Furthermore, concerns have been raised about the possible transfer of PFASs to humans through the consumption of edible parts of food plants. In this regard, to evaluate the potential toxic effects and the accumulation of perfluorooctanoic acid (PFOA) in edible plants, a pot experiment in greenhouse using three-week-old basil (Ocimum basilicum L.) plants was performed adding PFOA to growth substrate to reach 0.1, 1, and 10 mg Kg- 1 dw. RESULTS: After three weeks of cultivation, plants grown in PFOA-added substrate accumulated PFOA at different levels, but did not display significant differences from the control group in terms of biomass production, lipid peroxidation levels (TBARS), content of α-tocopherol and activity of ascorbate peroxidase (APX), catalase (CAT) and guaiacol peroxidase (POX) in the leaves. A reduction of total phenolic content (TPC) was instead observed in relation to the increase of PFOA content in the substrate. Furthermore, chlorophyll content and photochemical reflectance index (PRI) did not change in plants exposed to PFAS in comparison to control ones. Chlorophyll fluorescence analysis revealed an initial, rapid photoprotective mechanism triggered by PFOA exposure, with no impact on other parameters (Fv/Fm, ΦPSII and qP). Higher activity of glutathione S-transferase (GST) in plants treated with 1 and 10 mg Kg- 1 PFOA dw (30 and 50% to control, respectively) paralleled the accumulation of PFOA in the leaves of plants exposed to different PFOA concentration in the substrate (51.8 and 413.9 ng g- 1 dw, respectively). CONCLUSION: Despite of the absorption and accumulation of discrete amount of PFOA in the basil plants, the analysed parameters at biometric, physiological and biochemical level in the leaves did not reveal any damage effect, possibly due to the activation of a detoxification pathway likely involving GST.

Cultivation of Melilotus officinalis as a source of bioactive compounds in association with soil recovery practices.

Introduction: Melilotus officinalis is a Leguminosae with relevant applications in medicine and soil recovery. This study reports the application of Melilotus officinalis plants in soil recovery and as a source of bioactive compounds. Methods: Plants were cultivated in semiarid soil under four different fertilizer treatments, urban waste compost at 10 t/ha and 20 t/ha, inorganic fertilizer and a control (no fertilizer). Agronomic properties of soil (pH, EC, soil respiration, C content, macro- and microelements) were analyzed before and after treatment. Also, germination, biomass, element contents, and physiological response were evaluated. Metabolite composition of plants was analyzed through Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Results and discussion: Results showed a significant enhancement of the soil microbial activity in planted soils amended with compost, though there were no other clear effects on the soil physicochemical and chemical characteristics during the short experimental period. An improvement in M. officinalis germination and growth was observed in soils with compost amendment. Metabolite composition of plants was analyzed through Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Principal Component and Agglomerative Hierarchical Clustering models suggest that there is a clear separation of the metabolome of four groups of plants grown under different soil treatments. The five most important discriminative metabolites (annotated) were oleamide, palmitic acid, stearic acid, 3-hydroxy-cis-5-octenoylcarnitine, and 6-hydroxynon-7- enoylcarnitine. This study provides information on how the metabolome of Melilotus might be altered by fertilizer application in poor soil regions. These metabolome changes might have repercussions for the application of this plant in medicine and pharmacology. The results support the profitability of Melilotus officinalis cultivation for bioactive compounds production in association with soil recovery practices.

Morpho-physiological and molecular responses of Lepidium sativum L. seeds induced by bismuth exposure.

Bismuth (Bi) is considered a "green metal" as its toxicity has been reported to be lower than other metals, particularly lead. Even though the low presence in the environment, an increase of Bi concentrations in soil and wastewater is predictable due to its enhanced uses for many industrial and medical applications. Therefore, given the little literature on the matter, particularly in plants, information on the effects of Bi on living organisms is needed. In this study, seeds of garden cress (Lepidium sativum L.), a model plant for ecotoxicological assays (OECD), were exposed to increasing Bi concentrations (0 to 485 mg L-1 Bi(NO3)3·5H2O in deionised water) in petri plates. After 72 h, the percent germination index (GI%) revealed no effects at the lowest Bi concentrations, while a slight toxicity occurred at 242 and 485 mg L-1 Bi nitrate. A significant reduction of the root length was observed in Bi-treated seedlings, especially at the highest Bi concentrations. Consistently, the Alkaline Comet Assay revealed a genotoxic effect induced by Bi exposure in garden cress seedlings. A Bi concentration-dependent metal accumulation in plantlets was also observed, with a Bi concentration higher than 1200 mg kg-1 found in plantlets at the highest Bi concentration assayed. The toxicity effects observed in the study were discussed, as contribution to the expansion of knowledge on Bi ecotoxicity and genotoxicity in plants.

Ecotoxicological and genotoxic effects of dimethyl phthalate (DMP) on Lemna minor L. and Spirodela polyrhiza (L.) Schleid. plants under a short-term laboratory assay.

The environmental occurrence of phthalates (PAE) is of great concern for the ecosystem and human health. Despite of their recognized toxicity on biota, a lack of knowledge is still present about the effects of PAE on plants. In this scenario, the effects of dimethyl phthalate (DMP) on duckweed plants (Lemna minor L. and Spirodela polyrhiza (L.) Schleid.), two model plant species for ecotoxicological and trophic studies, were investigated. Under a 7-day lab assay, morphological (biometric indicators), physiological (pigment content and photosynthetic performance) and molecular (DNA damage) parameters were studied. No effects were observed at growth and physiological level in both plants at 3 and 30 mg/L DMP. On the contrary, at 600 mg/L DMP, a concentration used for plant acute toxicity studies, a remarkable growth inhibition and pigment content and photosynthetic parameters reduction compared to control were observed in both plants species, particularly in Spirodela. Alkaline Comet assay in 24 h-treated plants revealed a genotoxic damage induced by DMP, particularly relevant in Spirodela. These results described for the first time the adverse effects exerted by DMP on aquatic plants, contributing to highlight the environmental risk associated to the presence of this compound in the aquatic ecosystem.

Evaluation of morpho-physiological traits and contaminant accumulation ability in Lemna minor L. treated with increasing perfluorooctanoic acid (PFOA) concentrations under laboratory conditions.

There is increasing concern about the effects of releasing emerging contaminants (i.e. endocrine-disrupting chemicals, pharmaceuticals, personal-care products and flame retardants) into the environment. Particular attention is being paid to perfluoroalkyl substances (PFAS) because of their persistence and bioaccumulation, especially in the aquatic environment. In this paper, we present results of a study aimed at evaluating the effects of different perfluorooctanoic acid (PFOA) concentrations (2, 20 and 200 µg/L) on morpho-physiological traits in Lemna minor L. plants. The accumulation of PFOA in the plant's tissues was also monitored. L. minor was selected as a model plant for ecotoxicological studies, and we performed a seven-day assay for this investigation. The results highlight the lack of inhibitory effects on biometric parameters such as mean frond area, total frond number, multiplication rate, doubling time of frond number and average specific growth rate, for each of tested PFOA concentrations. Also, at photosynthetic level, physiological measurements showed that chlorophyll content and electron transport rate (ETR) were not affected by the exposure to PFOA. Remarkably, the chlorophyll fluorescence images, used for the first time in a study on PFOA, evidenced no impairment to the photosynthetic efficiency, measured by the maximum quantum yield of photosystem II (PSII) photochemistry (Fv/Fm), the quantum efficiency of PSII photochemistry (ΦPSII) and the non-photochemical quenching (NPQ) over the leaf surface of PFOA-treated plants, in comparison to control. Quantification of PFOA in the growth medium at the end of the seven-day test revealed no statistically different concentrations in plates with or without L. minor plants. We detected increasing PFOA accumulation in plant tissues, in accordance with the PFOA concentrations in the medium. Therefore, the L. minor plants were capable of taking up and accumulating PFOA. The ecological impact of the environmentally relevant PFOA concentrations tested in this work on biological organisms of the aquatic environment is discussed.

Phytoremediation and bioremediation of polychlorinated biphenyls (PCBs): state of knowledge and research perspectives.

This review summarizes the bioremediation and phytoremediation technologies proposed so far to detoxify PCB-contaminated sites. A critical analysis about the potential and limits of the PCB pollution treatment strategies by means of plants, fungi and bacteria are elucidated, including the new insights emerged from recent studies on the rhizosphere potential and on the implementation of simultaneous aerobic and anaerobic biodegradation processes. The review describes the biodegradation and phytoremediation processes and elaborates on the environmental variables affecting contaminant degradation rates, summarizing the amendments recommended to enhance PCB degradation. Additionally, issues connected with PCB toxicology, actual field remediation strategies and economical evaluation are discussed.