Clinical usefulness of brain-derived neurotrophic factor and visinin-like protein-1 in early diagnostic tests for acute stroke.

OBJECTIVES: Lack of a rapid biochemical test for acute stroke is a limitation in the diagnosis and management of acute stroke. The aim of this study is to evaluate the efficacy of BDNF and VILIP-1 as diagnostic markers in acute ischemic stroke and as predictors of mortality. METHODS: The study included 75 patients with acute ischemic stroke older than 18 years. During the same period, 28 normal controls were recruited from the hospital ED. Blood samples were collected from all patients at admission to determine the levels of VILIP-1 and BDNF. RESULTS: The mean VILIP-1 levels in the study and control groups were 0.547 ±â€¯0.081 and 0.515 ±â€¯0.035 ng/mL, respectively, and the difference was not significant (p = 0.071). The mean BDNF levels in the study and control groups were 3.89 ±â€¯2.05 ng/mL and 14.9 ±â€¯4.7 ng/mL, respectively, and the level was significantly (p < 0.0001) lower in the stroke patients. CONCLUSION: The BDNF level showed a significant ability to discriminate stroke and control patients but did not predict mortality. The VILIP-1 level showed insignificant ability to discriminate stroke patients and again did not predict mortality.

Diagnostic value of serum glial fibrillary acidic protein and S100B serum levels in emergency medicine patients with traumatic versus nontraumatic intracerebral hemorrhage.

BACKGROUND: Glial fibrillary acidic protein (GFAP) is a brain-specific astroglial protein that is released into the blood soon after traumatic brain injury by mature astrocytes. S100B is rapidly released into the cerebrospinal fluid and bloodstream after brain damage. We compared the serum concentrations of these proteins in patients with severe head trauma (bleeding and/or fracture) or nontraumatic intracerebral hemorrhage and healthy individuals. MATERIALS AND METHODS: The study included 63 patients (33 males and 30 females) with traumatic cerebral hemorrhage and/or cranial bone fractures or nontraumatic cerebral hemorrhage and 30 healthy control subjects. The reasons for attending the emergency department were as follows: fall from a height (n = 32), traffic accident (n = 18), nontraumatic intracerebral hemorrhage (n = 6), animal kick to the head (n = 4), and blow to the head (n = 3). RESULTS: Of the 63 patients included in the study, 33 (52.4%) were male and 30 (47.6%) were female. Of the 30 healthy controls, 12 (40%) were male and 18 (60%) were female. The average age of the patients was 27 years (range, 1 month to 86 years) and the average age of the control group was 21 years (range, 18-30 years). The mean serum GFAP concentrations were 86.37 ng/mL in the patients and 38.07 ng/mL in the controls (P < 0.05). The mean serum S100B concentrations were 428.37 pg/mL in the patients and 103.44 pg/mL in the controls (P < 0.05). Eight (12.7%) patients died in the hospital; of those, the mean GCS score was 4.6, and the mean GFAP and S100B levels were 127.8 ng/mL and 860.6 pg/mL, respectively. CONCLUSION: The GFAP and S100B concentrations were significantly higher in patients with traumatic or nontraumatic brain injury than in healthy individuals, indicating that serum levels of these biomarkers may provide an alternative to computed tomography for the diagnosis of brain injury.

A new method based for diagnosis of breast cancer cells from microscopic images: DWEE--JHT.

In these days, there are many various diseases, whose diagnosis is very hardly. Breast cancer is one of these type diseases. In this paper, accuracy diagnosis of normal, benign, and malign breast cancer cell were found by combining mean success rates Jensen Shannon, Hellinger, and Triangle measure which connected with each other. In this article, an diagnostic method based on feature extraction Discrete Wavelet Entropy Energy (DWEE) and Jensen Shannon, Hellinger, Triangle Measure (JHT) Classifier for diagnosis of breast cancer. This diagnosis method is called as DWEE--JHT this paper. With this diagnosis method have found optimal feature subset using discrete wavelet transform feature extraction. Then these convenient features are given to Jensen Shannon, Hellinger, Triangle Measure (JHT) classifier. Then, between classifiers which are Jensen Shannon, Hellinger, and triangle distance have been validated the measures via relationships. Afterwards, breast cancer cells are classified using Jensen Shannon, Hellinger, and Triangle distance. Mean success rate of 16 feature vector with Jensen Shannon classifier is found % 97.81. Mean success rate of 16 feature vector with Hellinger classifier is found % 97.75. Mean success rate of 16 feature vector with Triangle classifier is found % 97.87. By averaging of results obtained from these 3 classifiers are found as 97.81 % average of accuracy diagnosis.

Investigation of thermal treatment on selective separation of post consumer plastics prior to froth flotation.

Plastics have become the widely used materials because of their advantages, such as cheapness, endurance, lightness, and hygiene. However, they cause waste and soil pollution and they do not easily decompose. Many promising technologies are being investigated for separating mixed thermoplastics, but they are still uneconomical and unreliable. Depending on their surface characteristics, these plastics can be separated from each other by flotation method which is useful mineral processing technique with its low cost and simplicity. The main objective of this study is to investigate the flotation characteristics of PET and PVC and determine the effect of plasticizer reagents on efficient plastic separation. For that purpose, various parameters such as pH, plasticizer concentration, plasticizer type, conditioning temperature and thermal conditioning were investigated. As a result, PET particles were floated with 95.1% purity and 65.3% efficiency while PVC particles were obtained with 98.1% purity and 65.3% efficiency.

Mixed ligand-silver(I) complexes with anti-inflammatory agents which can bind to lipoxygenase and calf-thymus DNA, modulating their function and inducing apoptosis.

A new mixed ligand-silver(I) complex of formula [Ag(tpp)(2)(p-Hbza)] (1) (p-HbzaH = 4-hydroxybenzoic acid and tpp = triphenylphosphine) has been synthesized and characterized by elemental analysis, mp, vibrational spectroscopy (mid- and far-FT-IR), (1)H-NMR, UV-vis, ESI-MS spectroscopic techniques and X-ray crystallography. Complex 1 and the already known mixed ligand-silver(I) complexes of formulae [Ag(tpp)(2)(salH)] (2) (salH(2) = salicylic acid or 2-hydroxy-benzoic acid) and {[Ag(tpp)(3)(asp)](dmf)} (3) (aspH = o-acetylsalicylic acid) were used for the clarification of the cytostatic activity mechanism. Thus, 1-3 were tested for their in vitro cytotoxic activity against leiomyosarcoma (LMS) and human breast adenocarcinoma (MCF-7) cells with trypan blue and Thiazolyl Blue Tetrazolium Bromide (MTT) assays. For both cell lines, complexes 1-3 were found to be more active than cisplatin. Due to the morphology of the LMS cells after incubation with 1-3, the type of cell death was evaluated by flow cytometry assay and DNA fragmentation. The results show that LMS cells undergo programmed cell death (apoptosis). DNA binding tests indicate the ability of complexes 1-3 to modify the activity of the cells. The binding constants of 1-3 towards calf-thymus DNA (CT-DNA) ((27.7 ± 7.9) × 10(4) (1), (13.3 ± 6.5) × 10(4) (2) and (11 ± 2.8) × 10(4) (3) M(-1)) indicate strong interaction. Moreover, the influence of complexes 1-3 on the catalytic peroxidation of linoleic acid to hydroperoxylinoleic acid by the enzyme lipoxygenase (LOX) was kinetically studied. Finally, docking studies on DNA binding interactions were performed.

Bilateral investigation of the anatomical relationships of the external branch of the superior laryngeal nerve and superior thyroid artery, and also the recurrent laryngeal nerve and inferior thyroid artery.

The relationships of both the external branch of superior laryngeal nerve with the superior thyroidal artery and the recurrent laryngeal nerve with the inferior thyroidal artery were examined on the fixed cadavers of 4 adult women and 26 adult men. A total of 32 external branches of superior laryngeal nerve, 16 on each side, were dissected. When left and right sides were assessed altogether, 71.9% were medial to the artery while 28.1% were in between the branches. No nerve was found to be lateral to the artery. Thus the type in which the nerve is exposed to surgical trauma was found to be present in 28.1% of the cases. Only 12 of the cadavers could be assessed for symmetry and three fourths were found to have bilaterally symmetric relationship. A total of 52 Recurrent Laryngeal Nerves, 27 on the left and 25 on the right side were dissected. When bilateral symmetry was assessed in 21 cadavers, one third of the Recurrent Laryngeal Nerves were found to be bilaterally symmetrical. Inferior Thyroidal Artery was missing in 4 sides; bilaterally in one cadaver and unilaterally in 2 cadavers. Thus, artery-nerve relationship was assessed on 48 sides, 25 on the left and 23 on the right. On the right, 39.2% were in between the branches while 30.4% were anterior to the artery and 30.4% were posterior. On the left, 52% were in between the branches whereas 44% were posterior and 4% was anterior to the artery. Thus the position of the nerve in between the branches had the highest incidence while the anterior position had the lowest, the differences being statistically insignificant.

Cerebral vasculitis and ischaemic stroke in Behçet's disease: report of one case and review of the literature.

INTRODUCTION: Behçet's disease (BD) is a multisystemic, recurrent, inflammatory disorder. Neurological involvement is well-known but cerebral vasculitis and ischaemic stroke are unusual. CASE DESCRIPTION: A 43-year-old male patient presented with acute left hemiparesis, he had recurrent oral aphthae and scrotal ulcerations. Two episodes of transient brainstem ischaemia and an episode of right hemiparesis were reported in the past 2 years. Cranial magnetic resonance (MR) imaging showed a right striatocapsular infarction and multiple segmental stenosis, fusiform enlargement and beading of the arteries of the polygone of Willis were seen on angiography. Cerebro-spinal fluid (CSF) examination disclosed lymphocytic pleocytosis. Skin pathergy test was positive. A diagnosis of BD with cerebral vasculitis was made and immunosuppressive therapy was started. Some improvement of the arterial lesions on MR angiography and normalization of CSF were observed after 1 year of treatment. DISCUSSION: Low grade chronic meningo-encephalitis is the core neuropathological process in neuro-Behçet's disease. Nevertheless BD is a systemic disease known to cause vasculitis and can exceptionally lead to cerebral vasculitis and brain infarction. While BD is usually not part of the differential diagnosis of cerebral vasculitis, it should be borne in mind especially in endemic areas of the disease and in patients from these areas.