Characteristics of Piglets Born by Two Highly Prolific Sow Hybrids.

High piglet mortality constitutes a welfare challenge in Danish organic pig production with almost one in three piglets dying before weaning. Piglet characteristics such as birth weight, rectal temperature and intrauterine growth restriction (IUGR) affect piglet survival. Due to differences in breeding goals, these characteristics may be expected to differ between sow hybrids. Thus, the aims of the present study were (1) to investigate piglet characteristics in two highly prolific sow hybrids and (2) to study to which extent the aforementioned characteristics affect piglet mortality. Forty-nine sows (22 DanBred and 27 Topigs Norsvin) were followed in their first two parities. Sows were housed outdoors and gave birth in huts. On day 1 postpartum ( pp ) piglets were individually marked, weighed, their rectal temperature was recorded and they were scored for IUGR. Weight and rectal temperature were recorded again 3 days pp. Principal component analyses were conducted to explore relationships among variables. Early piglet death grouped with IUGR, lower rectal temperature and weight on day 1 pp. Late mortality grouped with increasing litter size and DanBred hybrid. Whilst, Topigs Norsvin hybrid grouped with increasing rectal temperature day 3 pp, longer crown to rump length, higher weight and more teats on the sow. Results of the statistical analyses showed that Topigs Norsvin piglets were heavier 1 and 3 days pp (p < 0.001) compared to DanBred piglets. Furthermore, Topigs Norsvin piglets had a higher rectal temperature than DanBred on day 1 pp (p = 0.023). The risk of IUGR depended on an interaction between sow hybrid and parity (p = 0.023). DanBred sows gave birth to more piglets (18.2 ± 0.6) than Topigs Norsvin sows (15.7 ± 0.5, p = 0.003), however, DanBred sows had fewer teats than Topigs Norsvin sows. Weight on day 1 pp affected both the odds of stillbirth (p < 0.001) and live born death (p < 0.001). Lower rectal temperature day 1 pp (p < 0.001) increased the odds of live born death. In conclusion, the investigated hybrids differed in several piglet characteristics related to piglet mortality. Use of sows giving birth to heavier and fewer piglets in the litter may thus be a useful tool to reduce piglet mortality in pig production with outdoor farrowing.

Causes of preweaning mortality in organic outdoor sow herds.

The aims of the current study were to identify the causes of preweaning piglet mortality and investigate the effect of season and parity on the proportions of mortality causes. The study was conducted in nine herds over a one-year period, and dead piglets were collected for necropsy once during each of the four seasons. In total, 2672 piglets were necropsied of which 60% were live-born but died post-partum (pp), 32% were stillborn and 8% were euthanised. Intra-partum deaths accounted for 88% of stillborn piglets. Eighty-one percent of necropsied live-born piglets died within four days pp. Half of these had a body weight of <1 kg at the time of death. Crushed and starved piglets accounted for 83% and 9% of mortalities until day four pp, respectively. Thirty-three percent of the crushed piglets (age zero to four days) had not suckled prior to death and the proportion of these piglets was lower in summer compared to the remaining part of the year (OR = 0.6, 95% CI [0.5;0.9]) and was higher for multiparous sows compared to first parity sows (OR = 1.7, 95% CI [1.1;2.6]). In conclusion, sow parity and season affected the proportion of crushed piglets before four days of age. Furthermore, the group of crushed piglets was heterogeneous and consisted of both heavy well-fed piglets and small piglets with empty stomachs. Thus, management implementations to lower mortality may differ depending on sow parity, season and individual piglet characteristics.

In vitro activity of ceftaroline against mecC-positive MRSA isolates.

Ceftaroline is a new cephalosporin with activity against methicillin-resistant Staphylococcus aureus (MRSA). A collection of 17 clinical and veterinary mecC-positive MRSA isolates was tested to evaluate the in vitro efficacy of ceftaroline against recently emerged mecC-MRSA isolates. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of ceftaroline for the 17 isolates were determined by broth microdilution using the methodology and interpretive criteria of the Clinical and Laboratory Standards Institute (CLSI). Additional susceptibility tests were performed using ceftaroline M.I.C.Evaluator (M.I.C.E.™) strips. All isolates showed susceptibility according to CLSI breakpoints, with MICs of ceftaroline ranging from 0.125mg/L to 0.25mg/L. MBCs were identical or up to a twofold dilution step higher. In conclusion, all tested isolates, from various sources and belonging to several clonal complexes (CCs), but predominantly to CC130, were found to be susceptible to ceftaroline. Ceftaroline could thus be an option for the treatment of mecC-MRSA infections.

Effect of infectious dose and season on development of hemorrhagic pneumonia in mink caused by Pseudomonas aeruginosa.

Hemorrhagic pneumonia is an acute and fatal disease of farmed mink caused by Pseudomonas aeruginosa. The pathogenesis of this disease has not yet been resolved. Mink are the only animals known to be susceptible to acute, contagious, and fatal lung infections caused by P. aeruginosa. The purpose of this study was to investigate the correlation between dose-response and season of infection and to clarify whether Danish mink are carriers of P. aeruginosa on their nasal mucosa during the season for hemorrhagic pneumonia. To elucidate the pathogenesis of the disease, an infectious dose-response trial was carried out on adult mink and mink kits, both in the season for hemorrhagic pneumonia (November) as well as out of season (July). It proved difficult to infect mink via the intra-nasal route. Only 4 out of 60 infected mink developed clinical disease and were euthanized, all of them in November, illustrating that predisposing factors in the mink itself and not infectious dose might be crucial for disease development. We were able to culture P. aeruginosa from the nasal cavity of the clinically healthy experimental mink 8 d after inoculation. This indicated that the mink can carry P. aeruginosa on their nasal mucosa without developing the disease. It was not possible, however, to culture P. aeruginosa from the nasal cavity of clinically healthy mink obtained from farms in November, which indicates that the organism is not a normal part of the nasal mucosal flora of mink.

La pneumonie hémorragique est une maladie aiguë et fatale du vison d'élevage causée par Pseudomonas aeruginosa. La pathogénie de cette maladie n'a pas encore été résolue. Les visons sont les seuls animaux connus à être susceptible à l'infection pulmonaire aiguë, contagieuse et fatale causée par P. aeruginosa. Le but de ce travail était d'étudier la corrélation entre la dose-réponse et la saison d'infection et de clarifier si les visons danois sont porteurs de P. aeruginosa sur leur muqueuse nasale durant la saison pour la pneumonie hémorragique. Afin d'élucider la pathogénie de la maladie, un essai de dose infectieuse-réponse a été réalisé chez des visons adultes et des jeunes visons, durant la saison pour la pneumonie hémorragique (novembre) aussi bien qu'hors-saison (juillet). Il s'est avéré difficile d'infecter les visons via la voie intra-nasale. Seulement 4 des 60 visons infectés ont développé la maladie clinique et ont été euthanasiés, tous en novembre, illustrant ainsi que des facteurs prédisposants chez le vison lui-même et non la dose infectante pourraient être cruciaux pour le développement de la maladie. Nous avons été en mesure de cultiver P. aeruginosa à partir de la cavité nasale des visons expérimentaux cliniquement sains 8 j après l'inoculation. Ceci indique que le vison peut être porteur de P. aeruginosa au niveau de la muqueuse nasale sans que la maladie ne se développe. Il n'a pas été possible toutefois de cultiver P. aeruginosa de la cavité nasale de visons cliniquement sains obtenus des fermes en novembre, ce qui indique que le microorganisme ne fait pas partie de la flore normale de la muqueuse nasale du vison.(Traduit par Docteur Serge Messier).

Detection of mecC-positive Staphylococcus aureus (CC130-MRSA-XI) in diseased European hedgehogs (Erinaceus europaeus) in Sweden.

Recently, a novel mec gene conferring beta-lactam resistance in Staphylococcus aureus has been discovered. This gene, mecC, is situated on a SCCmec XI element that has to date been identified in clonal complexes 49, 130, 425, 599 and 1943. Some of the currently known isolates have been identified from animals. This, and observations of mecA alleles that do not confer beta-lactam resistance, indicate that mec genes might have a reservoir in Staphylococcus species from animals. Thus it is important also to screen wildlife isolates for mec genes. Here, we describe mecC-positive Staphylococcus aureus (ST130-MRSA-XI) and the lesions related to the infection in two diseased free-ranging European hedgehogs (Erinaceus europaeus). One was found dead in 2003 in central Sweden, and suffered from S. aureus septicaemia. The other one, found on the island of Gotland in the Baltic Sea in 2011, showed a severe dermatitis and was euthanised. ST130-MRSA-XI isolates were isolated from lesions from both hedgehogs and were essentially identical to previously described isolates from humans. Both isolates carried the complete SCCmec XI element. They lacked the lukF-PV/lukS-PV and lukM/lukF-P83 genes, but harboured a gene for an exfoliative toxin homologue previously described from Staphylococcus hyicus, Staphylococcus pseudintermedius and other S. aureus of the CC130 lineage. To the best of our knowledge, these are the first reported cases of CC130-MRSA-XI in hedgehogs. Given that one of the samples was taken as early as 2003, this was the earliest detection of this strain and of mecC in Sweden. This and several other recent observations suggest that CC130 might be a zoonotic lineage of S. aureus and that SCCmec XI/mecC may have originated from animal pathogens.

Foodborne disease prevention and broiler chickens with reduced Campylobacter infection.

Studies have suggested that flies play a linking role in the epidemiology of Campylobacter spp. in broiler chickens and that fly screens can reduce the prevalence of Campylobacter spp. We examined the year-round and long-term effects of fly screens in 10 broiler chicken houses (99 flocks) in Denmark. Prevalence of Campylobacter spp.-positive flocks was significantly reduced, from 41.4% during 2003-2005 (before fly screens) to 10.3% in 2006-2009 (with fly screens). In fly screen houses, Campylobacter spp. prevalence did not peak during the summer. Nationally, prevalence of Campylobacter spp.-positive flocks in Denmark could have been reduced by an estimated 77% during summer had fly screens been part of biosecurity practices. These results imply that fly screens might help reduce prevalence of campylobacteriosis among humans, which is closely linked to Campylobacter spp. prevalence among broiler chicken flocks.