Amburana cearensis seed extract stimulates astrocyte glutamate homeostatic mechanisms in hippocampal brain slices and protects oligodendrocytes against ischemia.

BACKGROUND: Stroke is a leading cause of death and disability worldwide. A major factor in brain damage following ischemia is excitotoxicity caused by elevated levels of the neurotransmitter glutamate. In the brain, glutamate homeostasis is a primary function of astrocytes. Amburana cearensis has long been used in folk medicine and seed extract obtained with dichloromethane (EDAC) have previously been shown to exhibit cytoprotective activity in vitro. The aim of the present study was to analyse the activity of EDAC in hippocampal brain slices. METHODS: We prepared a dichloromethane extract (EDAC) from A. cearensis seeds and characterized the chemical constituents by 1H and 13C-NMR. Hippocampal slices from P6-8 or P90 Wistar rats were used for cell viability assay or glutamate uptake test. Hippocampal slices from P10-12 transgenic mice SOX10-EGFP and GFAP-EGFP and immunofluorescence for GS, GLAST and GLT1 were used to study oligodendrocytes and astrocytes. RESULTS: Astrocytes play a critical role in glutamate homeostasis and we provide immunohistochemical evidence that in excitotoxicity EDAC increased expression of glutamate transporters and glutamine synthetase, which is essential for detoxifying glutamate. Next, we directly examined astrocytes using transgenic mice in which glial fibrillary acidic protein (GFAP) drives expression of enhanced green fluorescence protein (EGFP) and show that glutamate excitotoxicity caused a decrease in GFAP-EGFP and that EDAC protected against this loss. This was examined further in the oxygen-glucose deprivation (OGD) model of ischemia, where EDAC caused an increase in astrocytic process branching, resulting in an increase in GFAP-EGFP. Using SOX10-EGFP reporter mice, we show that the acute response of oligodendrocytes to OGD in hippocampal slices is a marked loss of their processes and EDAC protected oligodendrocytes against this damage. CONCLUSION: This study provides evidence that EDAC is cytoprotective against ischemia and glutamate excitotoxicity by modulating astrocyte responses and stimulating their glutamate homeostatic mechanisms.

Socioeconomic inequalities in alcohol consumption in Argentina: Comparative analysis from 2009, 2013, and 2018.

BACKGROUND: The present study investigates the socioeconomic inequalities in alcohol use and harmful drinking in Argentina, a middle-income beer- and wine-producing country with high levels of alcohol consumption. METHODS: Data from the last three waves (2009, 2013, and 2018) of the National Risk Factors Survey were used. Each wave comprised samples of 34,732, 32,365, and 29,224 individuals, respectively. Outcome measures included the prevalence of alcohol use and heavy episodic drinking (HED). The Erreygers concentration index was employed to measure the socioeconomic inequalities. RESULTS: HED prevalence among drinkers increased from 17 % in 2009 to 25 % in 2018. Overall, the results showed significant pro-rich inequality for alcohol use and pro-poor inequality for HED, indicating that while a smaller proportion of the most disadvantaged population consumed alcohol, harmful drinking tended to concentrate among them. Pro-rich inequality for alcohol use was deeper in the population aged 25+, particularly among women. HED among males aged 35+ showed the highest inequality against lower income groups. During the period analyzed, the group aged 18-24 years had the highest HED prevalence as well as the greatest increase in this measure; it tended to be equally distributed across socioeconomic groups, presenting no socioeconomic inequality by 2018. CONCLUSIONS: These results highlight the importance of the assessment and characterization of the most exposed population to alcohol and harmful drinking. Regardless of their socioeconomic status, the young population was identified as a group for targeted interventions because of its greater alcohol exposure and the potential growth in economic and social burdens.

Assessment of the Highest Stress Concentration Area Generated on the Mandibular Structure Using Meshless Finite Elements Analysis.

Frequently, the oral cavity area can be affected by different diseases, so the patient needs to be submitted to surgery to remove a specific region of the mandibular. A complete or partial discontinuity of the mandibular bone can cause direct or indirect forces variations during the mastication. The dental prosthesis is an alternative to generate an aesthetic or functional solution for oral cavity lesions. However, they can be wrongly designed, or they can lose the adjustment during their useful life, deteriorating the patient's condition. In this work, the influence of the fixation components position for a dental prosthesis will be studied based on the finite element method. By means, it is possible to determine the area of the highest stress concentration generated on the mandibular structure. The temporomandibular image obtained by computational tomography was used as a 3D graphic whole model because in the medical area the morphological factors are extremely important. Vertical loads of 50, 100, 150 and 200 N were applied in three different regions: in the whole buccal cavity, simultaneously in the left and right laterals and only in the right lateral, to determine the values of von Mises stress in the mandible. These results were compared between three finite element software packages (Ansys®, SolidWorks® and Inventor®) and a meshless software (SimSolid®). They showed similar behaviors in the highest mechanical stress concentration in the same regions. Regarding the stress values, the percentage error between each software package was less than 10%. The use of SimSolid® software (meshless) proved to be better at identifying the higher stress generated by the dental prosthesis in the facial skeleton, so its computational efficiency, due to its geometric complexity, was highlighted.

Neonatal meningitis: a multicenter study in Lima, Peru. / Meningitis neonatal: estudio multicéntrico en Lima, Perú.

OBJECTIVE: To determine the incidence and the clinical, bacteriological and cerebrospinal fluid characteristics of neonatal meningitis in Lima hospitals. MATERIALS AND METHODS: An observational, multicenter study was conducted in six hospitals in the city of Lima during 1 year of epidemiological surveillance. RESULTS: The cumulative hospital incidence was 1.4 cases per 1000 live births. A total of 53 cases of neonatal meningitis were included, 34% (18/53) were early and 66% (35/53) late. The associated maternal factors were meconium-stained amniotic fluid and urinary tract infection. Insufficient prenatal check-ups were found in 58.8% (30/51). The most associated neonatal factor was sepsis. The main symptoms were fever, irritability, hypoactivity and respiratory distress. Pleocytosis in cerebrospinal fluid (CSF) was significant, without predominance of polymorphonuclear lymphocytes (PMN), hypoglycorrhagia and proteinorrhagia. The most frequent pathogens isolated were Escherichia coli and Listeria monocytogenes. CONCLUSIONS: The hospital incidence of neonatal meningitis was 1.4 per 1000 live births, being ten times higher in preterm infants. Breathing difficulty was the most frequent symptom in the early stage, while fever and irritability in the late stage. CSF showed pleocytosis without predominance of PMN. The most frequent germs were Escherichia coli and Listeria monocytogenes. Ventriculitis and hydrocephalus were the most common neurological complications.

OBJETIVO: Determinar la incidencia y las características clínicas, bacteriológicas y del líquido cefalorraquídeo de la meningitis neonatal en hospitales de Lima. MATERIALES Y MÉTODOS: Se realizó un estudio observacional, multicéntrico en seis hospitales de la ciudad de Lima, con una vigilancia epidemiológica durante un año. RESULTADOS: La incidencia acumulada hospitalaria fue de 1,4 casos por mil nacidos vivos. Fueron incluidos 53 casos de meningitis neonatal, 34% (18/53) fueron tempranos y 66% (35/53) tardíos. Los factores maternos asociados fueron líquido amniótico meconial e infección de tracto urinario. El 58,8% (30/51) presentó controles prenatales insuficientes. El factor neonatal más asociado fue sepsis. Los principales síntomas fueron fiebre, irritabilidad, hipoactividad y dificultad respiratoria. En el líquido cefalorraquídeo (LCR) se destacó la pleocitosis, sin predominio de polimorfonucleares (PMN), hipoglucorraquia y proteinorraquia. Los patógenos aislados con mayor frecuencia fueron Escherichia coli y Listeria monocytogenes. CONCLUSIONES: La incidencia hospitalaria de meningitis neonatal fue de 1,4 por mil nacidos vivos, siendo diez veces mayor en prematuros. La dificultad respiratoria fue el síntoma más frecuente en la forma temprana, mientras que la fiebre e irritabilidad en la forma tardía. El LCR mostró pleocitosis sin predominio de PMN. Los gérmenes más frecuentes fueron Escherichia coli y Listeria monocytogenes. La ventriculitis e hidrocefalia fueron las complicaciones neurológicas más comunes.

Meningitis neonatal: estudio multicéntrico en Lima, Perú / Neonatal meningitis: a multicenter study in Lima, Peru

RESUMEN Objetivo: Determinar la incidencia y las características clínicas, bacteriológicas y del líquido cefalorraquídeo de la meningitis neonatal en hospitales de Lima. Materiales y métodos: Se realizó un estudio observacional, multicéntrico en seis hospitales de la ciudad de Lima, con una vigilancia epidemiológica durante un año. Resultados: La incidencia acumulada hospitalaria fue de 1,4 casos por mil nacidos vivos. Fueron incluidos 53 casos de meningitis neonatal, 34% (18/53) fueron tempranos y 66% (35/53) tardíos. Los factores maternos asociados fueron líquido amniótico meconial e infección de tracto urinario. El 58,8% (30/51) presentó controles prenatales insuficientes. El factor neonatal más asociado fue sepsis. Los principales síntomas fueron fiebre, irritabilidad, hipoactividad y dificultad respiratoria. En el líquido cefalorraquídeo (LCR) se destacó la pleocitosis, sin predominio de polimorfonucleares (PMN), hipoglucorraquia y proteinorraquia. Los patógenos aislados con mayor frecuencia fueron Escherichia coli y Listeria monocytogenes. Conclusiones: La incidencia hospitalaria de meningitis neonatal fue de 1,4 por mil nacidos vivos, siendo diez veces mayor en prematuros. La dificultad respiratoria fue el síntoma más frecuente en la forma temprana, mientras que la fiebre e irritabilidad en la forma tardía. El LCR mostró pleocitosis sin predominio de PMN. Los gérmenes más frecuentes fueron Escherichia coli y Listeria monocytogenes. La ventriculitis e hidrocefalia fueron las complicaciones neurológicas más comunes.

ABSTRACT Objective: To determine the incidence and the clinical, bacteriological and cerebrospinal fluid characteristics of neonatal meningitis in Lima hospitals. Materials and methods: An observational, multicenter study was conducted in six hospitals in the city of Lima during 1 year of epidemiological surveillance. Results: The cumulative hospital incidence was 1.4 cases per 1000 live births. A total of 53 cases of neonatal meningitis were included, 34% (18/53) were early and 66% (35/53) late. The associated maternal factors were meconium-stained amniotic fluid and urinary tract infection. Insufficient prenatal check-ups were found in 58.8% (30/51). The most associated neonatal factor was sepsis. The main symptoms were fever, irritability, hypoactivity and respiratory distress. Pleocytosis in cerebrospinal fluid (CSF) was significant, without predominance of polymorphonuclear lymphocytes (PMN), hypoglycorrhagia and proteinorrhagia. The most frequent pathogens isolated were Escherichia coli and Listeria monocytogenes. Conclusions: The hospital incidence of neonatal meningitis was 1.4 per 1000 live births, being ten times higher in preterm infants. Breathing difficulty was the most frequent symptom in the early stage, while fever and irritability in the late stage. CSF showed pleocytosis without predominance of PMN. The most frequent germs were Escherichia coli and Listeria monocytogenes. Ventriculitis and hydrocephalus were the most common neurological complications.

Occurrence of relevant mycotoxins in food commodities consumed in Chile.

The aims of this study were to analyse the occurrence of aflatoxins, aflatoxin M1 (AFM1), fumonisins, ochratoxin A (OTA), patulin (PAT), zearalenone (ZEN) and deoxynivalenol (DON) in foodstuffs consumed in Chile between 2008 and 2017 and to estimate the contribution of main contaminated foodstuff in human exposure by the probable daily intake (PDI) estimation. In 9 years of surveillance, 2020 food samples were analysed with an occurrence of 18.2% and with 2.7% of the samples being over the Chilean regulation. The occurrence of mycotoxins in food were 16% for aflatoxins, 6% for AFM1, 30% for OTA, 12% for DON, 7% for PAT, 21% for fumonisins and 2% for ZEN. The estimated median PDI of DON because of bread consumption was 129.2 ng/kg bw/day for children and 96.0 ng/kg bw/day in adults. Median PDI because of capsicum consumption was 0.006 ng/kg bw/day for OTA and 0.005 ng/kg bw/day for aflatoxins. Median PDI of aflatoxins was estimated at 0.02 ng/kg bw/day for spices and 0.04 ng/kg bw/day for nuts consumption. In children, the median PDI of AFM1 for dairy consumption was 0.07 ng/kg bw/day. The derived margin of exposure (MoE) values ranged from 1133 to 8500 suggested that aflatoxins would be of public health concern. The PDI of the other mycotoxins did not show a health risk. This is the first survey of mycotoxins in food made in Chile; further research is needed to improve surveillance and guidelines based on national risk assessments and considering sensitive population groups.

[Update in diagnosis and early intervention of Autistic Spectrum Disorder]. / Actualización en diagnóstico e intervención temprana del Trastorno del Espectro Autista.

Autism Spectrum Disorder (ASD) is a neurodevelopmental condition that affects the social commu nication and behavior areas. Its symptomatology display heterogeneity and a wide range of functio nality levels in each child. In the last decade, significant advances have been made in the early detec tion of risk signs, favoring early diagnosis. This has allowed access to interventions that capitalize neuroplasticity of this stage of development, raising the possibility of mitigating the full manifesta tion of the disorder. The objective of this update is to review early diagnostic tools and early inter vention models and to analyze how to implement evidence-based interventions in a health context in a country like Chile.

Actualización en diagnóstico e intervención temprana del Trastorno del Espectro Autista / Update in diagnosis and early intervention of Autistic Spectrum Disorder

Resumen: El Trastorno del Espectro Autista (TEA) es una alteración del neurodesarollo que afecta las áreas de comunicación social y conducta, las cuales se manifiestan de manera heterogénea en cada niño y con una amplia gama de niveles de funcionalidad. En la última década se han hecho avances significativos en la detección temprana de señales de riesgo, favoreciendo la realización de diagnósticos precoz. Esto ha permitido el acceso a intervenciones que capitalizan la neuroplasticidad de esta etapa del desarrollo, planteando la posibilidad de mitigar la completa manifestación del trastorno. Los objetivos de esta actualización son revisar herramientas de diagnóstico precoz y modelos de intervención temprana, y analizar cómo implementar intervenciones basadas en la evidencia en un contexto sanitario de un país como Chile.

Abstract: Autism Spectrum Disorder (ASD) is a neurodevelopmental condition that affects the social commu nication and behavior areas. Its symptomatology display heterogeneity and a wide range of functio nality levels in each child. In the last decade, significant advances have been made in the early detec tion of risk signs, favoring early diagnosis. This has allowed access to interventions that capitalize neuroplasticity of this stage of development, raising the possibility of mitigating the full manifesta tion of the disorder. The objective of this update is to review early diagnostic tools and early inter vention models and to analyze how to implement evidence-based interventions in a health context in a country like Chile.

Preferencias alimentarias durante el recreo escolar: Niños de primaria de 9 a 10 años / Food preferences during lunch break: Elementary school children from 9 to 10 years

Resumen La alimentación de los niños durante el horario escolar es fundamental, por lo que a fin de adecuar la oferta alimentaria, es indispensable conocer cuáles son las preferencias alimentarias del alumnado. Así, el propósito de este estudio fue identificar los motivos de las preferencias y el consumo de alimentos durante el recreo escolar. Participaron 38 niños de 9-10 años de edad (55% niñas y 45% niños), alumnos de una escuela primaria pública de tiempo completo. Este estudio etnográfico comprendió el registro de alimentos consumidos y preferidos habitualmente, así como las narrativas de los niños, a través de observación dirigida, entrevistas no estructuradas y elaboración de dibujos. Se encontró que las preferencias alimentarias de los niños están estrechamente vinculadas al contexto familiar y los sentimientos ligados a éste. No obstante, en dichas preferencias influyen también la educación alimentaria recibida (escolar y extra-escolar), así como la disponibilidad-restricción de alimentos dentro de la escuela. En conclusión, aunque las y los niños podían no consumir en el recreo escolar su alimento favorito, adaptaban sus preferencias en función de los alimentos disponibles en la escuela, de modo que la selección variaba en función de la percepción y ponderación de las propiedades organolépticas del alimento (e.g., sabor, consistencia, temperatura).

Abstract Feeding children during school hours is essential, so to adapt the food supply, it is necessary to know what students' food preferences are. Thus, the purpose of this study was to identify the reasons for preferences and food consumption during school breaks. Thirty-eight children with an age range of 9 to 10 years (55% girls and 45% boys), from a full-time public primary school participated. This ethnographic study comprised the recording of commonly consumed and preferred foods, as well as children's narratives, through directed observation, unstructured interviews and drawings. It was found that children's food preferences are closely linked to the family context and the feelings linked to them. However, these preferences also influence the food education received (school and extra-school), as well as the availability-restriction of food within the school. In conclusion, although children could not consume their favorite food at school, they adapted their preferences according to the foods available at school, so the selection varied based on the perception and weighting of the organoleptic properties of the food (e.g., taste, consistency, temperature).

Highly clarithromycin-resistant Helicobacter pylori infection in asymptomatic children from a rural community of Cajamarca-Peru.

OBJECTIVE: The objective of this study was to determine the prevalence of clarithromycin-resistant Helicobacter pylori in asymptomatic children in a rural community of Cajamarca (northern Peru). RESULTS: Helicobacter pylori was detected in 17.2% (49/285) of the samples. Unboiled water consumption the most frequent associated factor in patients with positive PCR for H. pylori infection (93.9%). Clarithromycin resistant mutations were found in 79.6% (39/49) of the positive samples for H. pylori. The most frequent mutation was A2142G (46.9%), followed by the double-mutation A2142G-A2143G (28.6%).

Comportamiento y percepción del dolor en niños colombianos sometidos a electromiografía de superficie

Introducción: La Electromiografía-EMG de superficie evalúa la función muscular. Es poco utilizada como examen de rutina para valorar los músculos periorales y masticatorios, debido a que algunos consideran que puede ser molesto para el paciente pediátrico. Objetivo: Describir la percepción de dolor y el comportamiento en niños colombianos con y sin labio y paladar fisurado durante el examen de electromiografía de superficie en músculos periorales y masticatorios. Métodos: Previa aprobación del comité de ética (acta-009 2013p) y firma de consentimiento y asentimiento informado. Se realizó el examen en 69 niños entre 5-15 años: 45 con labio y paladar hendido (23-fisura unilateral, 22-fisura bilateral) y 24 sin hendidura. Se aplicaron las escalas de Wong Baker (Dolor) y Frankl (comportamiento). Resultados: 66 niños (95,65%) presentaron comportamiento Tipo 4 y 3 niños (4,35%) mostraron comportamiento Tipo 3 al inicio del examen. Al final del procedimiento, el 100% presentó comportamiento tipo 4. La percepción del dolor con la escala Wong Baker mostro ningún dolor en 62 niños (89,86%) y solo un poco de dolor en 7 (10,14%). Conclusiones: Los menores no manifiestan cambios importantes en su comportamiento por dolor o molestias durante el procedimiento de electromiografía de superficie.

The Surface electromyography-EMG evaluates muscular function. It is seldom used as a routine examination to assess perioral and masticatory muscles as it is considered to cause the pediatric patient discomfort. Objective: Describe the perception of pain and behavior displayed by Colombian children with and without cleft lip and palate during the surface electromyography test performed on perioral and masticatory muscles. Methods: Prior approval by the ethics committee (act-009-2013p) and an informed consent and assent signature were obtained. The exam were performed on 69 children between 5 and 15 years; 45 with cleft lip and palate (23 with unilateral cleft, 22 with bilateral cleft) and 24 children without cleft. The Wong Baker (pain) and Frankl (behavior) scales were used. Results: At the beginning of the exam, 66 children (95.66 %) displayed type 4 behavior and 3 children (4.35 %) displayed type 3 behavior. At the end of the procedure, 100 % of the children displayed type 4 behavior. When using the Wong Baker scale, the perception of the pain showed that 62 patients (89.86 %) felt no pain and 7 patients (10.14 %) felt just a little pain. Conclusions: Children do not show significant changes in their behavior - or pain and discomfort during the procedure of surface electromyography.

Anti-Taenia solium monoclonal antibodies for the detection of parasite antigens in body fluids from patients with neurocysticercosis.

Neurocysticercosis (NCC), an infection of the brain by Taenia solium (Ts) cysts, is the most common cause of adult-onset epilepsy in developing countries. Serological testing consists primarily of varying methods to detect antibodies in body fluids and more recently antigen (Ag) detection assays to identify individuals or animals with viable parasites. Antigen assays currently in use employ monoclonal antibodies (mAbs) raised against T. saginata, which have known cross reactivity to animal cestodes but are highly specific in human samples. We produced, characterized and tested 21 mAbs raised against T. solium whole cyst antigens, vesicular fluid or excretory secretory products. Reactivity of the TsmAbs against specific cyst structures was determined using immunofluorescence and immunohistochemistry on histological sections of Ts muscle cysts. Four TsmAbs reacted to vesicular space alone, 9 to the neck and cyst wall, one to the neck and vesicular space and 7 to the neck, cyst wall and vesicular space. An in-house ELISA assay to detect circulating Ts antigen, using the TsmAbs as capture antibodies and a rabbit polyclonal anti-Ts whole cyst antibody as a detector antibody demonstrated that eight of the 21 TsmAbs detected antigens in known NCC-positive human sera and three of these also in urine samples. Reactivity was expressed as normalized ratios of optical densities (OD positive control/OD negative control). Three TsmAbs had ratios >10 and five between 2 and 10. The TsmAbs have potential utility for the diagnosis and post-treatment monitoring of patients with viable NCC infections.

Tumor neuroendocrino en cérvix uterino: reporte de caso / Neuroendocrine tumor of the uterine cervix: case report

Los tumores neuroendocrinos de cuello uterino son extremadamente raros. Las mujeres con diagnóstico de carcinoma neuroendocrino de células pequeñas del cuello uterino tienen mayor frecuencia de metástasis en los ganglios linfáticos, invasión linfovascular, recurrencia y peor pronóstico en comparación con aquellos con otros tipos de neoplasias cervicales. Se presenta el caso de una mujer de 58 años, con un tiempo de enfermedad de seis años antes del ingreso, caracterizado por sangrado vaginal irregular posmenopáusica, además de sintomatología relacionada a anemia crónica. En el examen ginecológico, se evidenció tumoración de 4 cm que ocupaba tercio superior de vagina y protruía por el cérvix. Fue diagnosticado como mioma abortivo y enviada a estudio anatomopatológico. El resultado fue carcinoma neuroendocrino de células pequeñas grado III en el 90% y carcinoma epidermoide en el 10%. La paciente fue sometida a histerectomía radical más salpingo-ooferectomía bilateral y linfadenectomía pélvica bilateral y para-aortica. El estudio anatomopatológico de la pieza quirúrgica encontró endometrio y miometrio comprometido por neoplasia maligna. Parametrios, anexos y ganglios linfáticos se encontraron libres de neoplasia. A la microscopía el resultado fue carcinoma neuroendocrino grado III (carcinoma de células pequeñas, infiltrante), con extensa embolia linfovascular. El estudio de inmunohistoquímica arrojó sinaptofisina positivo en las áreas con diferenciación neuroendocrina.

Neuroendocrine tumors of the cervix are extremely rare. Women diagnosed with small cell neuroendocrine carcinoma of the cervix have a higher frequency of metastases in the lymph nodes, lymphovascular invasion, recurrence and worse prognosis compared to those with other types of cervical neoplasia. We report the case of a 58-year-old female, with a history of six years of postmenopausal irregular vaginal bleeding, in addition to symptoms related to chronic anemia. Gynecological examination showed a tumor of 4 cm that occupied the upper third of the vagina and protruded through the cervix initially diagnosed as an abortifacient myoma, and sent to histopathology study. 90% of the tumor was small cell neuroendocrine carcinoma grade III, and the remaining 10% was squamous cell carcinoma. The patient underwent into a radical hysterectomy plus bilateral salpingo-oophorectomy, and bilateral pelvic and para-aortic lymphadenectomy. Histopathologic examination of the surgical specimen found endometrium and myometrium compromised by malignancy. Parametrium, annexes and lymph nodes were free of neoplasia. At microscopy, the result was a grade III neuroendocrine carcinoma (small cell carcinoma, infiltrating), with extensive lymphovascular emboli. The immune-histochemical study showed synaptophysin positive in areas with neuroendocrine differentiation.

Evans blue staining reveals vascular leakage associated with focal areas of host-parasite interaction in brains of pigs infected with Taenia solium.

Cysticidal drug treatment of viable Taenia solium brain parenchymal cysts leads to an acute pericystic host inflammatory response and blood brain barrier breakdown (BBB), commonly resulting in seizures. Naturally infected pigs, untreated or treated one time with praziquantel were sacrificed at 48 hr and 120 hr following the injection of Evans blue (EB) to assess the effect of treatment on larval parasites and surrounding tissue. Examination of harvested non encapsulated muscle cysts unexpectedly revealed one or more small, focal round region(s) of Evans blue dye infiltration (REBI) on the surface of otherwise non dye-stained muscle cysts. Histopathological analysis of REBI revealed focal areas of eosinophil-rich inflammatory infiltrates that migrated from the capsule into the tegument and internal structures of the parasite. In addition some encapsulated brain cysts, in which the presence of REBI could not be directly assessed, showed histopathology identical to that of the REBI. Muscle cysts with REBI were more frequent in pigs that had received praziquantel (6.6% of 3736 cysts; n = 6 pigs) than in those that were untreated (0.2% of 3172 cysts; n = 2 pigs). Similar results were found in the brain, where 20.7% of 29 cysts showed histopathology identical to muscle REBI cysts in praziquantel-treated pigs compared to the 4.3% of 47 cysts in untreated pigs. Closer examination of REBI infiltrates showed that EB was taken up only by eosinophils, a major component of the cellular infiltrates, which likely explains persistence of EB in the REBI. REBI likely represent early damaging host responses to T. solium cysts and highlight the focal nature of this initial host response and the importance of eosinophils at sites of host-parasite interaction. These findings suggest new avenues for immunomodulation to reduce inflammatory side effects of anthelmintic therapy.

Prevalencia de fluorosis dental en infantes de 8 a 12 años de colegios públicos, Villavicencio 2013 / Prevalencia de fluorosis dental em infantes de 8 a 12 anos de colegios públicos, villavicencio 2013 / Prevalence dental fluorosis in 8 to 12 years old infants of public schools, villavicencio 2013

Objetivo: Determinar la prevalencia y severidad de fluorosis dental en población de 8 a 12 años de edad de los colegios públicos de Villavicencio, Colombia. Materiales y Métodos: Estudio descriptivo con participación voluntaria y previo consentimiento informado de los adultos responsables de 459 infantes de 8 a 12 años de edad, seleccionados de una población de 32.306 matriculados en los colegios públicos de Villavicencio, mediante muestreo probabilístico. Trabajo avalado por el Comité de Bioética de la Universidad Cooperativa de Colombia. El examen clínico fue realizado por 6 odontólogos previamente calibrados por un experto en el diagnóstico de fluorosis dental según índice de Thylstrup y Fejerskov (TFI) obteniéndose índices de Kappa > 0,80. Se realizó análisis exploratorio de datos (AED), distribución de frecuencias y estadística inferencial (prueba de asociación Chi2 de Pearson con α = 0,05), usando el programa estadístico SPSS versión 21.0 con licencia de la Universidad Cooperativa de Colombia. Resultados: La prevalencia de fluorosis (TFI ≥ 1) fue de 65,8% (n = 302) (IC 95% = 61,5% a 68,5%). El sexo masculino presentó una prevalencia del 51,3% (n = 155) (IC 95% = 47,3% a 55,3%) (p = 0,94). En la zona urbana la comuna de procedencia más afectada fue la número 6 con una prevalencia de 70,8% (IC 95% = 59,8% a 81,8%) (p = 0,84). El 31,4% de los niños presentaron un TFI = 2, el 23% un TFI = 1. Los dientes más afectados fueron los premolares superiores (48,2%) e inferiores (40,6%), seguidos de los incisivos laterales (40,2%). Conclusión: En la población infantil de 8 a 12 años de los colegios públicos de Villavicencio se presenta una elevada prevalencia de fluorosis con una severidad leve y moderada, representando un problema de salud pública que requiere una intervención de los organismos de control

Objective: To determine the prevalence and severity of dental fluorosis in the population aged 8-12 years old in public schools in Villavicencio, Colombia. Materials and Methods: A descriptive study, with voluntary participation and previous consent of the responsible adults, of 459 children aged 8 to 12 years, selected from a population of 32,306 students enrolled in public schools in Villavicencio, using probability sampling. This work is supported by the bioethics committee of the Universidad Cooperativa de Colombia. Clinical examination was carried out by six dentists previously calibrated by an expert in the diagnosis of dental fluorosis according Thylstrup and Fejerskov (TFI) obtaining Kappa indexes > 0.80. Exploratory data analysis (EDA), frequency distribution and inferential statistics (test partnership with Pearson Chi2 α = 0.05) were performed with SPSS version 21.0. Licensed by Universidad Cooperativa de Colombia. Results: The prevalence of fluorosis (TFI ≥ 1) was 65.8% (n = 302) (95% CI = 61.5% to 68.5%). Boys presented a prevalence of 51.3% (n = 155) (95% CI = 47.3% to 55.3%) (p = 0.94). In urban areas the most affected district of origin was No. 6 with a prevalence of 70.8% (95% CI = 59.8% to 81.8%) (p = 0,84). The 31.4% of children had a TFI = 2, 23% one TFI = 1. The most affected teeth were premolars (48.2%) (40.6%), followed by lateral incisors (40.2%). Conclusion: In the population of 8-12 years old children of public Villavicencio schools a higher prevalence of fluorosis with a mild to moderate severity is present, representing a public health problem that requires intervention by the supervisory bodies

Objetivo: Determinar a prevalência e severidade de fluorose dentaria em povoação de 8 a 12 anos de idade dos colégios públicos de Villavicencio, Colômbia. Materiais e Métodos: Estudo descritivo com participação voluntaria e prévio consentimento informado dos adultos responsáveis de 459 infantes de 8 a 12 anos de idade, selecionados de uma povoação de 32.306 matriculados nos colégios públicos de Villavicencio, através de uma amostra probabilística. Trabalho avaliado pelo Comitê de Bioética da Universidade Cooperativa de Colômbia. No teste clinico foi realizado por 6 dentistas previamente calibrados por um especialista no diagnostico de fluorose dentaria segundo índice de Thylstrup e Fejerskov (TFI) obtendo se índices de Kappa > 0,80. Realizou se analise exploratório de dados (AED), distribuição de freqüências estadística inferencial (prova de associação Chi2 de Pearson com α = 0,05), usando o programa estatístico SPSS versão 21.0 com licencia da Universidade Cooperativa de Colômbia. Resultados: A prevalência de fluorose (TFI ≥ 1) foi de 65,8% (n = 302) (IC 95% = 61,5% a 68,5%). O sexo masculino apresentou uma prevalência do 51,3% (n = 155) (IC 95% = 47,3% a 55,3%) (p = 0,94). Na zona urbana a comuna de procedência mais afetada foi a numero 6 com uma prevalência 70,8% (IC 95% = 59,8% a 81,8%) (p = 0,84). O 31,4 das crianças apresentaram um TFI = 2, o 23% um TFI = 1.Os dentes mais afeitados foram os pré- molares superiores (48,2%) e inferiores (40,6%), seguidos dos incisivos laterais (40,2%). Conclusão: Na povoação infantil de 8 a 12 anos dos públicos de Villavicencio apresenta se uma elevada prevalência de fluorose com uma severidade leve e moderada, representando um problema de saúde publica que requere uma intervenção dos organismos de controle

Identification and functional analysis of novel phosphorylation sites in the RNA surveillance protein Upf1.

One third of inherited genetic diseases are caused by mRNAs harboring premature termination codons as a result of nonsense mutations. These aberrant mRNAs are degraded by the Nonsense-Mediated mRNA Decay (NMD) pathway. A central component of the NMD pathway is Upf1, an RNA-dependent ATPase and helicase. Upf1 is a known phosphorylated protein, but only portions of this large protein have been examined for phosphorylation sites and the functional relevance of its phosphorylation has not been elucidated in Saccharomyces cerevisiae. Using tandem mass spectrometry analyses, we report the identification of 11 putative phosphorylated sites in S. cerevisiae Upf1. Five of these phosphorylated residues are located within the ATPase and helicase domains and are conserved in higher eukaryotes, suggesting a biological significance for their phosphorylation. Indeed, functional analysis demonstrated that a small carboxy-terminal motif harboring at least three phosphorylated amino acids is important for three Upf1 functions: ATPase activity, NMD activity and the ability to promote translation termination efficiency. We provide evidence that two tyrosines within this phospho-motif (Y-738 and Y-742) act redundantly to promote ATP hydrolysis, NMD efficiency and translation termination fidelity.

Cómo se diferencian clinicamente los diferentes tipos de demencia? / How to clinically differentiate the different types of dementia?

Esta monografía es una rápida y ágil revisión de las prácticas propuestas recientemente, a cerca de la forma en que se pueden diferenciar clínicamente los tipos más comunes de demencia. El objetivo de esta investigación, es ejercitar y conocer los diferentes medios de investigación, como internet, con que se cuenta para la futura realización de nuestra tesis, y además obtener conocimientos sobre patologías como éstas, muy comunes en la práctica médica diaria, que nos brinden más destrezas en nuestra formación médica. Es muy importante el reconocimiento de estas enfermedades, sus causas, factores de riesgo y síntomas asociados, para así poder llegar a un diagnóstico y tratamiento adecuados. Para esto, es necesario un correcto interrogatorio y un buen examen físico...