Eco-friendly monitoring of triclosan as an emerging antimicrobial environmental contaminant utilizing electrochemical sensors modified with CNTs nanocomposite transducer layer.

Environmental appearance of antimicrobials due to frequent use of personal care products as recommended by WHO can cause serious flare-up of antimicrobial resistance. In this work, three eco-friendly microfabricated copper solid-state sensors were developed for measuring triclosan in water. Multi-walled carbon nanotubes were incorporated in sensor 2 and 3 as hydrophobic conductive inner layer. Meanwhile, ß-cyclodextrin was incorporated in sensor 3 as an ionophore for selective binding of TCS in presence of interfering compounds. The obtained linear responses of sensors 1, 2 and 3 were (1 × 10- 8-1 × 10- 3 M), (1 × 10- 9-1 × 10- 3 M) and (1 × 10- 10- 1 × 10- 3 M), respectively. Limit of detection was 9.87 × 10- 9 M, 9.62 × 10- 10 M, and 9.94 × 10- 11 M, respectively. The miniaturized sensors were utilized for monitoring of triclosan in water samples.

Point-of-care diagnostics for rapid determination of prostate cancer biomarker sarcosine: application of disposable potentiometric sensor based on oxide-conductive polymer nanocomposite.

One of the most important reasons for an increased mortality rate of cancer is late diagnosis. Point-of-care (POC) diagnostic sensors can provide rapid and cost-effective diagnosis and monitoring of cancer biomarkers. Portable, disposable, and sensitive sarcosine solid-contact ion-selective potentiometric sensors (SC-ISEs) were fabricated as POC analyzers for the rapid determination of the prostate cancer biomarker sarcosine. Tungsten trioxide nanoparticles (WO3 NPs), polyaniline nanoparticles (PANI NPs), and PANI-WO3 nanocomposite were used as ion-to-electron transducers on screen-printed sensors. WO3 NPs and PANI-WO3 nanocomposite have not been investigated before as ion-to-electron transducer layers in potentiometric SC sensors. The designated sensors were characterized using SEM, XRD, FTIR, UV-VIS spectroscopy, and EIS. The inclusion of WO3 and PANI in SC sensors enhanced the transduction at the interface between the screen-printed SC and the ion-selective membrane, offering lower potential drift, a longer lifetime, shorter response time, and better sensitivity. The proposed sarcosine sensors exhibited Nernstian slopes over linear response ranges 10-3-10-7 M, 10-3-10-8 M, 10-5-10-9 M, and 10-7-10-12 M for control, WO3 NPs, PANI NPs, and PANI-WO3 nanocomposite-based sensors, respectively. From a comparative point of view between the four sensors, PANI-WO3 nanocomposite inclusion offered the lowest potential drift (0.5 mV h-1), the longest lifetime (4 months), and the best LOD (9.95 × 10-13 M). The proposed sensors were successfully applied to determine sarcosine as a potential prostate cancer biomarker in urine without prior sample treatment steps. The WHO ASSURED criteria for point-of-care diagnostics are met by the proposed sensors.

Sensitive Derivative Synchronous and Micellar Enhanced Ecofriendly Spectrofluorimetric Methods for the Determination of Atenolol, Diclofenac, and Triclosan in Drinking Tap Water.

BACKGROUND: Nowadays, emergence of unexpected contaminants in drinking water is a challenging environmental problem facing humanity. OBJECTIVE: Two eco-friendly spectrofluorimetric methods were proposed for the determination of three unexpected contaminants in drinking tap water. METHODS: The first method is first derivative synchronous spectrofluorimetric method which was developed for simultaneous determination of atenolol (ATN) and diclofenac (DCF) without prior separation at Δλ = 70 nm and at Δλ = 80 nm for ATN and DCF, respectively. The second method was based on using sodium dodecyl sulfate (SDS) as fluorescent enhancer of triclosan (TCS) native fluorescence. TCS exhibits enhanced fluorescence at λ emission = 600 nm upon excitation at λ excitation = 299.4 nm. Solid phase extraction was carried out in both methods. RESULTS: Linear calibration curves were obtained in concentration range of (4-3000 ng/mL) for ATN and (4-2000 ng/mL) for DCF, by measuring first derivative signal of fluorescence at 300 nm and 375.2 nm, respectively. TCS exhibits linear range (0.1-1 ng/mL) at 600 nm. Mean percentage recoveries were 101.04 ± 0.571, 99.66 ± 1.443, and 99.73 ± 0.566 for ATN, DCF, and TCS, respectively. CONCLUSIONS: Validation of both methods were performed according to the International Conference on Harmonization guidelines. Results obtained were statistically compared with published methods and no significant differences were found. The proposed methods' greenness is evaluated using analytical Eco-scale and Green Analytical Procedure Index. A greenness comparison with previously published methods has been performed. HIGHLIGHTS: Both methods were found to be eco-friendly and were successfully applied for the determination of the emerging contaminants in drinking tap water.

In situ monitoring of triclosan in environmental water with subnanomolar detection limits using eco-friendly electrochemical sensors modified with cyclodextrins.

The environmental emergence of unexpected contaminants has gained the attention of the scientific community. A broad spectrum antimicrobial compound named triclosan (TCS) was detected in the environment as an emerging contaminant. Owing to its inherent toxicity, we have proposed eco-friendly potentiometric liquid state sensors to be used for monitoring and quantifying TCS in environmental water samples. The proposed sensors have been optimized by modifying the inner filling solution using hydrophilic 2-hydroxypropyl ß-cyclodextrin as a complexing agent to be capable of minimizing the trans-membrane ion flux and hence improving the selective and sensitive determination of TCS in environmental matrices with low LOD values. The obtained linear response of the optimized sensor was (1 × 10-9 to 1 × 10-5 M) compared to the control sensor (1 × 10-8 to 1 × 10-4 M). The obtained limit of detection (LOD) value was found to be 9.86 × 10-10 M compared to 9.78 × 10-9 M of the control sensor. The modification of the inner filling solution of the sensor with 2-hydroxypropyl ß-cyclodextrin improves not only its sensitivity but also its response time to be only 5 seconds. The electrical performance of the proposed sensor was evaluated following IUPAC recommendations. Both the pH and temperature effects were studied and optimized. Two different greenness assessment tools, Analytical Eco-scale and Green Procedure Index, were adopted upon the evaluation of the proposed sensors' greenness.