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1.
Bull Exp Biol Med ; 174(6): 806-809, 2023 Apr.
Article de Anglais | MEDLINE | ID: mdl-37160604

RÉSUMÉ

We studied the neutrophils and monocytes obtained from 37 patients with various inflammatory diseases such as psoriasis, acute infectious process in the abdominal cavity (acute appendicitis/abscess of the abdominal cavity, and acute cholecystitis), acute pancreatitis, and post-COVID syndrome after mild COVID infection. The number and the morphological structure of neutrophil extracellular traps (NET) as well as the effect of IgG on NET were examined. NET were visualized and counted by fluorescence microscopy with fluorescent dye SYBR Green. All the studied types of inflammation were accompanied by spontaneous formation of NET. After application of IgG, the number of NET doubled, their size increased, and transformation of net-like traps into the cloud forms was observed. The clouds form structure of the network is not capable of capturing pathogens with subsequent retraction, the products of its enzymatic degradation can be the factors of secondary alteration. The study results demonstrate a previously unknown mechanism of infection resistance.


Sujet(s)
COVID-19 , Pièges extracellulaires , Pancréatite , Humains , Pièges extracellulaires/métabolisme , Maladie aigüe , Pancréatite/métabolisme , COVID-19/métabolisme , Granulocytes neutrophiles/métabolisme , Immunoglobuline G/métabolisme
2.
Khirurgiia (Mosk) ; (12. Vyp. 2): 6-25, 2022.
Article de Russe | MEDLINE | ID: mdl-36562669

RÉSUMÉ

The article presents the work of a multidisciplinary team of experts from various fields of medicine to optimize the «Questionnaire for assessing chronic pelvic pain and pelvic organ dysfunction (QCPPD) of the Ryzhikh National Medical Research Centre for Coloproctology¼ for use in clinical practice. The survey of respondents was conducted from June 28 to September 28, 2021. As a result of this survey, by repeatedly making edits and clarifications during communication with respondents, the final version was obtained, which allows assessing the patient's subjective sensations by the nature and localization of pelvic pain, sensitivity disorders and pelvic organ function. The main objective of this Questionnaire is to differentiate patients with neurogenic pain from a huge number of patients with chronic pelvic pain. This aspect will allow a more targeted approach to the diagnosis and pathogenetically justified treatment of patients, including after appropriate instrumental examinations. The work of a multidisciplinary team implies a higher degree of objectification and terminological accuracy of the Questionnaire under discussion. The presented version of the «Questionnaire for assessing chronic pelvic pain and pelvic organ dysfunction (QCPPD) of the Ryzhikh National Medical Research Centre for Coloproctology¼ will be primarily used in coloproctological patients with pelvic pain problems and anal incontinence and obstructive defecation. Further studies will be directed to the clinical evaluation of the results of the work carried out.


Sujet(s)
Incontinence anale , Défaillance multiviscérale , Humains , Douleur pelvienne/diagnostic , Douleur pelvienne/étiologie , Constipation , Enquêtes et questionnaires
3.
Bull Exp Biol Med ; 164(5): 693-700, 2018 Mar.
Article de Anglais | MEDLINE | ID: mdl-29577184

RÉSUMÉ

Tissue damage in various diseases, hypoxic conditions, and some pathologies are associated with production of endogenous factors such as the soluble form of the surface receptor CD100, malonic dialdehyde, and arginase and their release into circulation. These factors modulate functional state of lymphocytes in the immune system: potentiate activation of B lymphocytes, activate synthesis and secretion of IL-25 and IL-17 cytokines, and suppress proliferative activity of T lymphocytes, thus modulating immunological reactivity of the organism. Reactions of innate and adaptive immunity develop against the background of changed immunological reactivity, which should be taken into account in the development of pathogenetically substantiated therapy.


Sujet(s)
Antigènes CD/métabolisme , Arginase/métabolisme , Sémaphorines/métabolisme , Animaux , Antigènes CD/génétique , Arginase/génétique , Lymphocytes B/immunologie , Lymphocytes B/métabolisme , Humains , Interleukine-17/métabolisme , Sémaphorines/génétique , Lymphocytes T/métabolisme
6.
Patol Fiziol Eksp Ter ; (1): 23-5, 2009.
Article de Russe | MEDLINE | ID: mdl-19382620

RÉSUMÉ

Latent sensitization is a risk factor of a clinically manifest atopic reaction. Investigation of a phenotypic composition of lymphocytes reveals function of the immune system in this condition. Indirect fluorescence assessed differential and activation lymphocyte antigens (CD4, CD3, CD8, CD16, CD20 and CD25, CD71, HLA-DR, CD95, CD23, CD54, respectively) in 15 patients with latent sensitization, 32 patients with atopic bronchial asthma and 22 healthy donors. The immune system demonstrated definite activation with involvement ofT- and B-links of the immune system. Lymphocyte activation in latent sensitization and bronchial asthma differs by high expression of CD95 apoptosis trigger receptor. Enhancement of Fas-mediated lymphocyte apoptosis may inhibit IgE synthesis and atopic pathology manifestation.


Sujet(s)
Asthme/immunologie , Hypersensibilité retardée/immunologie , Activation des lymphocytes/immunologie , Lymphocytes/immunologie , Adulte , Allergènes/immunologie , Antigènes CD/sang , Antigènes CD/immunologie , Asthme/sang , Études cas-témoins , Femelle , Humains , Hypersensibilité retardée/sang , Immunoglobuline E/sang , Immunoglobuline E/immunologie , Mâle , Adulte d'âge moyen , Pollen/immunologie , Tests cutanés
7.
Patol Fiziol Eksp Ter ; (1): 2-7, 2006.
Article de Russe | MEDLINE | ID: mdl-16607883

RÉSUMÉ

Three groups of activation antigens (receptors) of human peripheral blood lymphocytes (CD23, CD54--functional activation antigens, CD25 and CD71--early activation antigens and HLA-DR and CD95--late activation antigens) were studied in patients with inflammatory diseases of different types. All kinds of inflammation are independent of etiological factors and are associated with immune system activation. Inflammation induced by tissue injury or invasion of nonspecific microorganisms leads to a rapid rise in the count of lymphocytes expressing early markers of activation. Inhibition of inflammation occurs with a significant rise in peripheral blood lymphocytes with receptor of activation apoptosis CD95 (Fas-antigen). High number of lymphocytes with early markers of activation in line with high number of blood CD95+ lymphocytes take place in allergic and autoimmune diseases for a long time. Abnormal regulation of activation processes in lymphocytes in allergic and autoimmune diseases consists in the absence of lymphocyte activation inhibition in spite of high expression of CD95.


Sujet(s)
Antigènes de différenciation des lymphocytes T/analyse , Inflammation/immunologie , Activation des lymphocytes , Lymphocytes/immunologie , Adolescent , Adulte , Femelle , Humains , Système immunitaire/immunologie , Mâle , Adulte d'âge moyen
8.
Stomatologiia (Mosk) ; 84(3): 4-7, 2005.
Article de Russe | MEDLINE | ID: mdl-16007004

RÉSUMÉ

Systemic immunity was studied in patients with chronic generalized parodontitis. This group of patients had distinct changes in immunologic system: depression of T- and stimulation of B-cellular immunity without accumulation of plasma cells and initiation of effective humoral response. Increased peripheral blood number of lymphocytes expressing induction of apoptosis CD95 receptors and ligand for this receptor CD95L (Fas-L) can lead to intensification of lymphocyte apoptosis and may be the reason for T-cell deficit development. The results of the study confirm the important role of immune system disturbances in pathogenesis of chronic generalized parodontitis.


Sujet(s)
Apoptose , Lymphocytes B/immunologie , Parodontite/immunologie , Lymphocytes T/immunologie , Lymphocytes B/cytologie , Ligand de Fas , Humains , Immunité , Activation des lymphocytes , Glycoprotéines membranaires/analyse , Lymphocytes T/cytologie , Antigènes CD95/analyse
9.
Patol Fiziol Eksp Ter ; (1): 23-5, 2005.
Article de Russe | MEDLINE | ID: mdl-15801236
10.
Bull Exp Biol Med ; 138(1): 65-6, 2004 Jul.
Article de Anglais | MEDLINE | ID: mdl-15514726
19.
Patol Fiziol Eksp Ter ; (4): 33-6, 1993.
Article de Russe | MEDLINE | ID: mdl-7910390

RÉSUMÉ

Peripheral lymphocytes from 36 patients were used to study pathogenetic mechanisms of development of atopic bronchial asthma (ABA), which are associated with regulatory dysfunction of T lymphocytes. Models of determining immunoregulatory lymphocytic subpopulations (CD4+ and CD8+) and assessing the function of concanavalin A-induced immunoregulatory lymphocytes indicated that the reception of the immune mediators IL-2 and alpha-interferon was impaired in ABA patients. The paper gives the data on a significant drastic decrease in cAMP concentrations and an increase in cAMP levels in the peripheral lymphocytes in the patients. The findings suggest that there is an impaired turnover of cyclic nucleotides in the lymphoid cells of patients with atopy.


Sujet(s)
Asthme/immunologie , Lymphocytes T/immunologie , Adulte , AMP cyclique/métabolisme , Humains , Adulte d'âge moyen , Lymphocytes T/métabolisme
20.
Patol Fiziol Eksp Ter ; (6): 36-8, 1990.
Article de Russe | MEDLINE | ID: mdl-2082259

RÉSUMÉ

The mechanisms of the antianaphylactic effect of Saventol, a drug synthesized in the USSR, were studied on various experimental models. It was established that both in experiments on isolated organs and when given in injections to animals in vivo, Saventol produced a marked dose-dependent antianaphylactic effect. The results allow the antianaphylactic effect of Saventol to be linked with its antimediator effect realized through beta 2-adrenoceptors.


Sujet(s)
Salbutamol/usage thérapeutique , Anaphylaxie/traitement médicamenteux , Animaux , Cochons d'Inde
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