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Molecules ; 16(8): 7143-54, 2011 Aug 23.
Article de Anglais | MEDLINE | ID: mdl-21862956

RÉSUMÉ

Microbiological processes were used for chitin and chitosan production by Mucor circinelloides (UCP 050) grown in yam bean (Pachyrhizus erosus L. Urban) medium. The polysaccharides were extracted by alkali-acid treatment and structural investigations by X-ray diffraction, Fourier transform IR analysis, viscosity and thermal analysis by TG, DTG, and DTA were done. The highest biomass yield (20.7 g/L) was obtained at 96 hours. The highest levels of chitosan (64 mg/g) and chitin (500 mg/g) were produced at 48 and 72 hours, respectively. It was demonstrated that yam bean shows great potential as an economic medium and it is possible to achieve a good yield of chitosan with chemical properties that enable its use in biotechnological applications.


Sujet(s)
Chitine/métabolisme , Chitosane/métabolisme , Milieux de culture/composition chimique , Mucor/métabolisme , Pachyrhizus/composition chimique , Acétylation , Techniques de culture cellulaire en batch , Calorimétrie différentielle à balayage , Chitine/composition chimique , Chitosane/composition chimique , Fermentation , Diffraction sur poudre , Diffraction des rayons X
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