RÉSUMÉ
There is an international need for a large-scale human neutrophils antigen (HNA) antibody screening platform to minimize the risk of antibody-mediated transfusion-related acute lung injury. However, sourcing a substantial, reliable source of HNA, as well as the scarcity of well-characterized HNA antisera for validating new screening platforms, remain as major obstacles. This short communication presents an improved protocol for the effective use of HNA-expressing KY cells as a screening platform using eight well-characterized HNA antisera of a single defined specificity.
Sujet(s)
Anticorps/sang , Technique d'immunofluorescence/méthodes , Alloanticorps/sang , Isoantigènes/biosynthèse , Granulocytes neutrophiles/immunologie , Animaux , Anticorps/immunologie , Spécificité des anticorps , Bovins , Lignée cellulaire , Humains , Alloanticorps/immunologie , Isoantigènes/sang , Isoantigènes/immunologie , TransfectionRÉSUMÉ
Applying serologic procedures to the detection of RBC and lymphocyte antigens has facilitated the identification of granulocyte antigens with established clinical significance, which are now classified in the human neutrophil antigen system. Granulocyte alloantibodies and autoantibodies have been implicated in a variety of clinical conditions including alloimmune neutropenia, autoimmune neutropenia, febrile and severe pulmonary transfusion reactions, drug-induced neutropenia, refractoriness to granulocyte transfusions, and immune neutropenia after hematopoietic stem cell transplantation. Although the intrinsically fragile nature of granulocytes contributes to the inherent challenges of granulocyte serology, several advances in laboratory procedures have improved detection of granulocyte antibodies. This review will provide a current perspective about the importance and use of granulocyte serology for detection of granulocyte antibodies that have significant medical effects.
Sujet(s)
Granulocytes/immunologie , Alloanticorps/immunologie , Isoantigènes/immunologie , Neutropénie/immunologie , Anticorps monoclonaux/immunologie , Congrès comme sujet , Prévision , Génotype , Test d'histocompatibilité , Humains , Immunité acquise d'origine maternelle , Nouveau-né , Alloanticorps/sang , Isoantigènes/sang , Isoantigènes/classification , Isoantigènes/génétique , Neutropénie/induit chimiquement , Neutropénie/congénital , Neutropénie/étiologie , Tests sérologiques , Sérologie/organisation et administration , Terminologie comme sujet , Réaction transfusionnelleRÉSUMÉ
Transfusion-related acute lung injury (TRALI) is one of the leading causes of transfusion-associated mortality. The inadvertent transfusion of neutrophil antibodies can cause pulmonary transfusion reactions and TRALI. However, not all patients transfused with neutrophil antibodies experience transfusion reactions. A 22-year-old man with severe aplastic anaemia (SAA) experienced TRALI after a platelet transfusion. The donor was found to be alloimmunized to human neutrophil antigen (HNA)-3a, an antigen expressed by neutrophils from approximately 90% of Caucasians. Eleven other platelet components from this donor were transfused prior to this event and two caused reactions: one chills and one TRALI. Both episodes of TRALI occurred in the same male patient with SAA. The fact that one patient experienced TRALI following both exposures to anti-HNA-3a from the same donor whereas nine other recipients did not adds evidence to the observation that patient factors make a significant contribution to neutrophil antibody-mediated transfusion reactions.