Application of soy protein isolate-naringenin complexes as fat replacers in low-fat cream: Based on protein conformational changes, aggregation states and interfacial adsorption behavior.

In this study, changes in the structural and functional properties of soybean protein isolate (SPI)-naringenin (NG) complexes under different amounts of naringenin treatments were explored, elucidating the effect of the complexes as fat replacers at the 15 % substitution level on the properties of low-fat cream. Finally, the correlation between the structure and function of the complex and the properties of low-fat cream was further analyzed. The addition of NG promotes the increase of SPI aggregation and particle size, and reduces the interfacial tension of the complex. Meanwhile, at the mass ratio of 48:3, NG and SPI formed a dendritic network structure suitable for stabilizing cream. The fat properties of cream indicate that low-fat creams stabilized by appropriate proportions of SPI-NG complexes displayed small and dense fat crystal network structures. In addition, low-fat cream stabilized by the SPI-NG complexes have improved whipping time, overrun, firmness, storage stability and rheological properties compared to natural SPI. It is worth noting that the overall quality of the cream stabilized by the SPI-NG complex with a mass ratio of 48:3 was almost close to that of full-fat cream. Therefore, this study promotes the potential applications of protein-polyphenol complexes as fat replacers in the food industry.

Determining ideal offsets of spatially offset Raman spectroscopy for transcutaneous measurements-A Monte Carlo study.

Spatially offset Raman spectroscopy (SORS) is valuable for noninvasive bone assessment but requires a clearer understanding of how offset distances influence detection depth. To address this, our study devised a forward-adjoint Monte Carlo multi-layer (MCML) model to simulate photon paths in SORS, aiming to determine optimal offsets for various tissue types. We examined photon migration at offsets between 0 and 15 mm against layered phantoms of differing thicknesses and compositions to optimize the signal-to-noise ratio for bone layers. The findings highlight that optimal offsets are contingent on tissue characteristics: a metacarpal beneath 2.5 mm of tissue had an ideal offset of 6.7 mm, while a tibia with 5 mm of soft tissue required 10-11 mm. This precise calibration of SORS via MCML modeling promises substantial improvements in bone health diagnostics and potential for expansive medical applications.

Raman spectroscopic analysis for osteoporosis identification in humans with hip fractures.

Osteoporosis is a significant health concern. While multiple techniques have been utilized to diagnose this condition, certain limitations still persist. Raman spectroscopy has shown promise in predicting bone strength in animal models, but its application to humans requires further investigation. In this study, we present an in vitro approach for predicting osteoporosis in 10 patients with hip fractures using Raman spectroscopy. Raman spectra were acquired from exposed femoral heads collected during surgery. Employing a leave-one-out cross-validated linear discriminant analysis (LOOCV-LDA), we achieved accurate classification (90 %) between osteoporotic and osteopenia groups. Additionally, a LOOCV partial least squares regression (PLSR) analysis based on the complete Raman spectra demonstrated a significant prediction (r2 = 0.84, p < 0.05) of bone mineral density as measured by dual X-ray absorptiometry (DXA). To the best of our knowledge, this study represents the first successful demonstration of Raman spectroscopy correlating with osteoporotic status in humans.

Growth of ZnO nanorods/flowers on the carbon fiber surfaces using sodium alginate as medium to enhance the mechanical properties of composites.

The chemical inertness of the carbon fiber (CF) surface results in suboptimal mechanical properties of the prepared composites. To address this issue, we employed a combination of tannic acid and 3-aminopropyltriethoxysilane mixture (TA-APTES) grafted sodium alginate (SA) as a medium to enhance the interfacial properties of composites through the growth of ZnO nanoparticles on CF surfaces. ZnO nanolayers with rod-like and flower-like structures were obtained by adjusting the pH of the reaction system (pH = 10 and 12, respectively). Characterization results show that in comparison with the untreated CF composites, in the flexural strength, flexural modulus, interlaminar shear strength (ILSS) and interfacial shear strength (IFSS) of the as-prepared CF/TA-APTES/SA/ZnO10 (nanorods) composites were improved by 40.8 %, 58.4 %, 44.9 % and 47.8 %, respectively. The prepared CF/TA-APTES/SA/ZnO12 (nanoflowers) composite showed an increase in flexural strength, flexural modulus, ILSS and IFSS by 39.8 %, 63.6 %, 47.3 % and 48.2 %, respectively. These positive results indicate that the ZnO nanolayers increase the interfacial phase area and fiber surface roughness, thereby enhancing mechanical interlocking and load transfer between the fibers and resin matrix. This work provides a novel interfacial modification method for preparing CF composites used in longer and more durable wind turbine blades.

Morphology, surface characteristics and tribological properties of whey protein/chitosan composite particles and their fat replacing effect in O/W emulsion.

Whey protein isolate (WPI) and chitosan were used to fabricate WPI/chitosan composite particles at temperatures of 75 °C (WPI/chitosan-75) and 95 °C (WPI/chitosan-95). The morphologic structure, surface properties, and the resulting tribological characteristics of the particles were investigated. The composite particles showed larger particle size than pure WPI particles (WPI-75) (~ 509 nm), with WPI/chitosan-95 the largest (932 nm). WPI/chitosan-75 showed complete core-shell structure from microstructure results. The dispersion of WPI/chitosan-75 exhibited higher surface hydrophobicity but lower viscosity compared to WPI/chitosan-95. Tribological analysis revealed that WPI/chitosan composite particles showed dramatically lower friction coefficient (µ) than pure WPI particles at sliding speed <10 mm/s and WPI/chitosan-75 demonstrated superior lubrication effects. With the presence of artificial saliva, the µ of WPI-75 was greatly lowered at sliding speed <16 mm/s, while the values of WPI/chitosan-75 only showed a slight decrease at sliding speed <1 mm/s. Chitosan might have played the similar role as artificial saliva in lubricating on the hydrophobic surface. Moreover, the incorporation of 0.5 % WPI/chitosan-75 in the low-fat (5 %) oil-in-water emulsion led to even lower µ than full-fat (20 %) emulsion at sliding speed <10 mm/s. Hence, WPI/chitosan-75 exhibited promising potential as a fat replacement and biolubricant.

Feasibility of spectral CT-derived extracellular volume fraction for differentiating aldosterone-producing from nonfunctioning adrenal nodules.

OBJECTIVE: To assess the feasibility of spectral CT-derived extracellular volume (ECV) for differentiating aldosterone-producing nodules (APN) from nonfunctioning adrenal nodules (NFN). METHODS: Sixty-nine patients with biochemically and histologically confirmed unilateral APN (34) and NFN (35) as well as 23 patients with bilateral APN (19) and NFN (27) confirmed biochemically and by adrenal vein sampling (AVS) were enrolled in this retrospective study from October 2020 to April 2022. All patients underwent contrast-enhanced spectral CT of the adrenal glands with a 10-min delayed phase. The haematocrit level was measured within 2 days of CT. An iodine density map was derived from the delayed CT. The ECV fractions of the APN and NFN were calculated and compared in the test cohort of 69 patients with unilateral adrenal nodules. The optimal cut-off value was determined to evaluate the diagnostic efficacy of the ECV fraction for differentiating APN from NFN in the validation cohort of 23 patients with bilateral adrenal nodules. RESULTS: The ECV fractions of the APN (11.17 ± 4.57%) were significantly lower (p < 0.001) than that of the NFN (24.79 ± 6.01%) in the test cohort. At cut-off ECV value of 17.16%, the optimal area under the receiver operating characteristic curve was 0.974 (95% confidence interval: 0.942-1) with 91.4% sensitivity, 93.9% specificity, and 92.8% accuracy in the test cohort and 89.5% sensitivity, 96.3% specificity, and 93.5% accuracy in the validation cohort for differentiating APN from NFN. CONCLUSION: The spectral CT-derived ECV fraction can differentiate APN from NFN with high diagnostic performance. CLINICAL RELEVANCE STATEMENT: Spectral CT-derived extracellular volume fraction could accurately differentiate between adrenal aldosterone-producing nodules and nonfunctioning nodules. It might serve as a noninvasive alternative to adrenal vein sampling in primary aldosteronism patients with bilateral adrenal nodules. KEY POINTS: • Conventional CT cannot differentiate aldosterone-producing adrenal nodules from nonfunctioning nodules. • Extracellular volume of adrenal aldosterone-producing nodules was significantly lower than that of nonfunctioning nodules and normal adrenal glands. It can accurately differentiate between aldosterone-producing and nonfunctioning adrenal nodules. • Extracellular volume may be a novel, noninvasive biomarker alternative to adrenal vein sampling for determining the functional status of bilateral adrenal nodules in patients with primary aldosteronism.

[Association between HLA-DRB1 gene polymorphisms and genetic susceptibility of early-onset severe preeclampsia]. / HLA-DRB1åŸºå› å¤šæ€æ€§ä¸Žæ—©å‘åž‹é‡åº¦å­ç—«å‰æœŸé—ä¼ æ˜“æ„Ÿæ€§çš„ç ”ç©¶.

OBJECTIVES: To explore the correlation between the single nucleotide polymorphisms (SNP) of rs3135388, rs114293611 and rs142804168 of HLA-DRB1 gene and early-onset severe preeclampsia (sPE). METHODS: Blood samples were collected from 102 early-onset sPE mothers and their neonates (sPE group), as well as 120 normotensive mothers and their neonates (control group). Sanger sequencing was performed to compare the genotype distribution, allele frequencies, and differences in genotype distribution after maternal-infant compatibility between the two groups. RESULTS: Statistically significant differences in genotype distribution at rs114293611 of HLA-DRB1 gene were observed between sPE and control groups in both mothers and neonates (P<0.05). The frequency of the T allele at rs114293611 was higher in the sPE group of neonates than that in the control group (P<0.05), while no significant difference was found between the two groups of mothers (P>0.05). The maternal-infant genotype compatibility analysis showed significant differences in genotype distribution between sPE and control groups (P<0.05). There were no significant differences in genotype distribution and allele frequencies at rs3135388 and rs142804168 of HLA-DRB1 gene between the two groups of mothers and neonates (P>0.05). CONCLUSIONS: The SNP at rs114293611 of HLA-DRB1 gene may be associated with the development of early-onset sPE in mothers. Maternal-infant genotype compatibility abnormality at rs114293611 of HLA-DRB1 gene may be a predisposition factor for the development of sPE.

Physiochemical characteristics, morphology, and lubricating properties of size-specific whey protein particles by acid or ion aggregation.

To investigate the influence of particle characteristics on their lubricating capacity, microparticles of controlled size (~300, ~700, and ~1900 nm) were prepared from whey proteins using two different approaches: reducing the pH and increasing the calcium ion concentration. The physiochemical, morphological, and tribological properties of the two types of particles were determined. Both treatments pronouncedly decreased the absolute value of zeta-potential and surface hydrophobicity of whey proteins, with calcium ions showing a more severe effect on zeta-potential. The viscosity of the particle suspensions increased with particle size, and ion-induced samples showed higher viscosity than acid-induced ones. Morphology investigation revealed that particle aggregation and irregularity increased with particle size increase. Distinct lubricating behaviors were observed for the two particle types within different size ranges. Viscosity played a more important role in lubrication when the particle size was small, while particle characteristics became more dominant for large particles.

Strain Transfer Mechanism in Surface-Bonded Distributed Fiber Optic Sensors under Different Strain Fields.

Mastering the strain transfer mechanism in distributed fiber optic (DFO) sensors holds the key to analyzing strain measurement errors from DFO sensing systems. However, the impact of the monitored structure's strain distribution on the strain transfer mechanism in DFO sensors has often been overlooked in the existing research. To address this issue, a strain transfer model of surface-bonded DFO sensors with multilayered structures was established based on the shear lag theory. The closed-form solutions of the strain transfer coefficient of DFO sensors subjected to uniform, parabolic, single-linear gradient, and bilinear gradient strains were obtained. With a high-accuracy optical frequency-domain reflectometer (OFDR), the theoretical model was validated by laboratory tests. Upon parametric analysis, suggestions were further offered about designing and installing DFO sensors.

Healthcare services efficiency and its intrinsic drivers in China: based on the three-stage super-efficiency SBM model.

BACKGROUND: The purpose of this study is to examine the development of healthcare services efficiency in China since the reform of the healthcare system. By examining the development environment of healthcare services in China and examining the driving factors affecting the efficiency of healthcare services, we provide a reference for the future high-quality development of healthcare services in China. METHODS: A three-stage super-efficient slack-based measure (SBM) model with undesirable outputs was used to measure the efficiency of healthcae services in 31 Chinese provinces from 2009 to 2021, and a global Malmquist-Luenberger (GML) index was used to assess their spatiotemporal evolution characteristics and internal influencing mechanisms of healthcare services efficiency. RESULTS: The empirical results showed that the efficiency of China's healthcare services changed significantly from 2009-2014 and then remained stable. During the study period, the efficiency of healthcare services in the eastern region was higher than the national level, while it was lower in the western region. The results of the analysis of environmental factors indicated that an increase in population density reduced the redundancy of healthcare input resources and that economic development as well as an increase in government subsidies, contributed to an increase in the redundancy of healthcare input resources. The main contribution to the growth of healthcare sercices efficiency in China came from the technological innovation effect, and the growth was most significant in the western region. CONCLUSION: From 2009 to 2021, the efficiency of national healthcare services generally showed a slow upward trend, and the efficiency of healthcare services varied widely among regions. Under the existing environmental constraints, relevant departments in each region should strengthen technological innovation in healthcare services, completely focus on the regional catch-up effect, and promote the balanced development of regional health.

Controllable synthesis of lignin nanoparticles with antibacterial activity and analysis of its antibacterial mechanism.

As a kind of polyphenol substance, lignin is considered to have good biological activity and certain antibacterial properties. However, it is difficult to be applied because of its uneven molecular weight and difficulty in separation. In this study, by way of fractionation and antisolvent, we obtained lignin fractions with different molecular weight. Moreover, we increased the content of active functional groups and regulated microstructure of lignin, thereby increased lignin's antibacterial property. The classification of chemical components and the control of particle morphology also provided convenience for the exploration of lignin's antibacterial mechanism. The results showed that acetone with high hydrogen bonding ability could collect lignin with different molecular weights and increase the content of phenolic hydroxyl groups, up to 31.2 %. By adjusting the ratio of water/solvent (v/v) and stirring rate during the process of antisolvent, lignin nanoparticles (sphere 40-300 nm) with regular shape and uniform size can be obtained. Through observing the distribution of lignin nanoparticles in vivo and in vitro after co-incubation for different time, it could be found that lignin nanoparticles firstly damage structural integrity of bacterial cells externally, and then are swallowed into cells to affect their protein synthesis, which constitutes a dynamic antibacterial process.

Xenon attenuates hypoxic-ischemic brain damage by inhibiting autophagy in neonatal rats.

Xenon (Xe) is an inert, colorless and odorless heavy gas and has many biological functions. However, little is known about whether and how Xe can modulate hypoxic-ischemic brain damage (HIBD) in neonatal rats. This study employed a neonatal rat model to explore the potential effect of Xe on neuron autophagy and the severity of HIBD. Neonatal Sprague-Dawley rats were subjected to HIBD, randomized and treated with Xe or mild hypothermia (at 32 °C) for 3 h. The degrees of HIBD, neuron autophagy and the neuronal functions in some neonates from each group were tested by histopathology, immunochemistry, transmission electron microscopy, western blot, open-field and Trapeze tests at 3 and 28 days post-induction of HIBD, respectively. Compared with the Sham group, hypoxic-ischemia caused larger volumes of cerebral infarction and severe brain damage, and increased autophagosome formation and Beclin-1 and microtubule-associated protein 1A/1B-light chain 3 class II (LC3-II) expression in the brain of rats, accompanied by the defect in neuronal functions. In contrast, treatment with Xe and/or hypothermia significantly reduced infarct volumes and ameliorated neurological defects in the HIBD rats, particularly for the combination of Xe and hypothermia. Xe significantly mitigated the relative levels of Beclin-1 and LC3-II expression and autophagosome formation induced by HIBD in rats. Xe acted as a neuroprotective factor against HIBD, possibly by inhibiting the hypoxia-induced neuron autophagy in rats.

Measuring the efficiency of public hospitals: A multistage data envelopment analysis in Fujian Province, China.

Objective: The present study aimed to evaluate the operational efficiency of public hospitals in Fujian Province and the factors responsible for the inefficiency of these hospitals and provide relevant suggestions for health policymakers in allocating service resources. Method: In the first stage of the research, the variables affecting the efficiency of hospitals were extracted by qualitative and quantitative methods, including literature optimization, gray related analysis and gray clustering evaluation. In the second stage, the data envelopment analysis (DEA) method was used to evaluate the operational efficiency of 49 hospitals of different levels and types selected by sampling in 2020. Finally, a Tobit regression model with introduced institutional factors and background factors was established to study the main influencing factors of hospital inefficiency. Results: In the first stage, 10 input variables and 10 output variables necessary from the mangers' point of view were identified to test efficiency. In the second stage, the average comprehensive TE, PTE, and SE of 49 sample hospitals was 0.802, 0.888, and 0.902, respectively. 22.45% of these hospitals met the effective criteria, i.e., the overall effective rate was 22.45%. The low SE value of the hospital was the main reason hindering the improvement of the comprehensive efficiency value. The overall effective rate of secondary public hospitals (30.77%) was higher than that of tertiary public hospitals (19.44%), and the overall effective rate of public specialized hospitals (30%) was higher than that of general public hospitals (18.92%). Based on the third stage results, the bed occupancy rate (BOR) and the proportion of beds (POB) were major factors affecting the operation efficiency of grade III hospitals (p < 0.01). However, the operating efficiency of grade II hospitals was significantly affected by POB and regional per capita GDP(GDPPC) (p < 0.05). Moreover, the impact of BOR and GDPPC was positive, and POB was negatively correlated with hospital operation efficiency. Conclusions: The study results indicated that the overall operation efficiency of public hospitals in Fujian Province is low. This study revealed that intervention should be strengthened from a policy and management perspective to improve the operation efficiency of public hospitals.

Renal venous density in the arterial phase of contrast-enhanced CT predicts prognosis in septic shock.

OBJECTIVE: To evaluate a series of vascular parameters derived from abdominal dual-phase contrast-enhanced CT as predictors of 14-day mortality and AKI within 7 days in septic shock. METHODS: 144 patients with septic shock and 60 negative cases were included. The vascular parameters from CT were measured and calculated, including aortic density in arterial (Dena-A) and venous phase (Dena-V), renal vein density in arterial (Denrv-A) and venous phase (Denrv-V), and renal vein-to-aortic density ratio in arterial (DenRrv/a-A) and venous phase (DenRrv/a-V). The parameters were compared between patients and controls, and between patients with different clinical outcomes, and assessed for predictive value of 14-day mortality and AKI within 7 days. RESULTS: Patients with septic shock presented significantly lower Denrv-A (p < 0.001) and DenRrv/a-A (p = 0.002) levels than the controls. In the septic shock group, patients who died had significantly lower Denrv-A (p = 0.001) and lower DenRrv/a-A (p < 0.001) than those who survived. Patients who developed AKI had significantly lower Denrv-A (p < 0.001) and DenRrv/a-A (p = 0.011) than those who did not. Multivariate analysis suggested DenRrv/a-A as an independent predictor of 14-day mortality (OR 0.012; 95% confidence interval [CI]:0.002,0.086; p < 0.001) and Denrv-A as an independent predictor of AKI (OR 0.989;95% CI:0.982,0.997; p = 0.006). CONCLUSION: In septic shock, significant decreases in Denrv-A and DenRrv/a-A were associated with the onset of AKI and predicted higher 14-day mortality. ADVANCES IN KNOWLEDGE: The renal vein density and renal vein-aortic density ratio in arterial phase of dual-phase contrast-enhanced CT may serve as good predictors of AKI and mortality in septic shock.

Quantitative MRI in distinguishing bladder paraganglioma from bladder leiomyoma.

PURPOSE: To investigate the feasibility of quantitative MRI in the differentiation of bladder paraganglioma from bladder leiomyoma. METHODS: From 2014 to 2021, 11 patients with bladder paraganglioma and 14 patients with bladder leiomyoma confirmed by surgical pathology were retrospectively collected. All patients underwent multiparametric scanning with a 3.0 T MR system. Quantitative parameters including the SI-ratio on T1WI between the solid component of tumours and piriformis, ADC value and E-rate of the solid component of tumours were assessed. Independent sample t test or Mann-Whitney U test was used to compare these parameters between the two groups. The diagnostic efficiency of the parameters was examined using ROC curve analysis and the DeLong test. RESULTS: There were significant differences in SI-ratio on T1WI (P < 0.001), ADC value (P = 0.002) and the E-rate (P = 0.040) between the paraganglioma group and the leiomyoma group. The cutoff value of SI-ratio on 3 leiomyoma was 1.241, and the AUC was 1.000 (0.858-1.000). The cutoff value of the ADC value was 0.979 × 10-3mm2/s, and the AUC was 0.907 (0.717-0.987). The cutoff value of E-rate was 98.7%, and the AUC was 0.714 (0.495-0.878). The AUCs of the SI-ratio on T1WI and ADC value were significantly higher than the E-rate AUC (P = 0.015 and 0.034, respectively). CONCLUSION: Quantitative MRI can effectively distinguish bladder paraganglioma from bladder leiomyoma with the SI-ratio on T1WI or ADC value.

Cyclic synthesis of lignin anthraquinone electrolytes for aqueous redox flow batteries.

Lignin, which is rich in phenolic hydroxyl/methoxy groups as redox active groups, is a potential electrolyte material for aqueous redox flow batteries (ARFBs). This work demonstrated to the synthesis of lignin-derived electrolytes via cyclization with 1,4-dihydroxyanthraquinone (1,4-DHAQ), in the absence of hazardous or noble metal catalysts in mild conditions (0 °C, 1 atm). The structure of lignin anthraquinone derivatives (LAQDs) cyclized in basis alkaline solution was experimentally determined. An exhaustive comparative study was conducted with respect to the electrochemical properties, charging-discharging tests and cycling performances. The initially volumetric capacitance, the capacity retention rate and coulombic efficiency of two LAQDs were determined to be 148.0 mAh.L-1, 89.3 % and 99.0 % for coniferaldehyde-anthraquinone derivative [LAQD(G)], and 132.1 mAh.L-1, 81.2 % and 99.0 % for sinusaldehyde-anthraquinone derivative [LAQD(S)], respectively. The theoretical value calculated by DFT is consistent with the actual value. Such LAQDs can be used as organic electrolyte materials, which can overcome poor chemical stability of anthraquinone, while improving the electrochemical activity of lignin-based electrolyte materials. This technology provides a pathway to prepare organic electrolyte for the development of environment friendly and better energy storage performance electrolytes for ARFBs.

Adrenal heterogeneity in the arterial phase of contrast-enhanced CT predicts prognosis in septic shock: comparison with hollow adrenal gland sign.

PURPOSE: To evaluate the prognostic value of adrenal heterogeneity in the arterial phase in patients with septic shock, comparatively to the hollow adrenal gland sign (HAGS). METHODS: Totally 84 consecutive patients with septic shock (group S) were assessed retrospectively, and abdominal dual-phase contrast-enhanced CT was performed after the diagnosis of septic shock within one week. The patients were divided into two groups according to clinical outcome, including the survivor (group A, 41 cases) and death (group B, 43 cases) groups. Fifty negative cases were matched as the control (group C). The incidence of the HAGS in patients with septic shock (group S) was statistically analyzed. The average values of left adrenal density (Den-A and Den-V) and their standard deviations (SDDen-A and SDDen-V) in dual phases were measured. The above parameters were compared between groups A and B as well as with group C. The parameters were assessed for their predictive values of mortality in septic shock, comparatively to the HAGS. RESULTS: Compared with group C, group S presented significantly higher Den-A (P = 0.003) and SDDen-A (P < 0.001). There were significantly higher SDDen-A (P < 0.001) in group B compared with group A. The incidence of the HAGS was about 27.4% (23/84) in group S. The sensitivity and specificity in predicting poor prognosis in patients with septic shock were 78% and 85% with SDDen-A, at a cut-off value of 28.64, respectively. The sensitivity and specificity were 41% and 88% for the HAGS, respectively. The area under ROC curve (AUC) was significantly greater for SDDen-A compared with the HAGS (0.820 vs. 0.670, P < 0.001). CONCLUSION: Adrenal heterogeneity in the arterial phase can predict prognosis in patients with septic shock; the larger the SDDen-A, the poorer the prognosis. The predictive efficiency of adrenal heterogeneity in the arterial phase is better than the HAGS.

Improvement and Performance Evaluation of a Dual-Probe Heat Pulse Distributed Temperature Sensing Method Used for Soil Moisture Estimation.

Large-scale measurements of soil moisture play a critical role in many fields, such as agriculture, hydrology, and engineering. The distributed temperature sensing (DTS) technology, based on a dual-probe heat pulse (DPHP), is a novel approach to realizing large-scale soil moisture estimation. However, the application of the method is limited by the complex optical cable layout, calculation algorithm, and lack of standardized heating strategy. In this paper, an improved DPHP-DTS method considering the soil bulk density was proposed. The measurement accuracy of the DPHP-DTS method under different heating strategies was studied in laboratory experiments, and its long-term stability in regard to the monitoring of soil moisture during natural evaporation in different soils was tested. The results show that the improved DPHP-DTS method can accurately measure the soil moisture, and the fitting algorithm can reduce the error caused by the accuracy of the DTS temperature measurement under the low-power heating strategy. Its measurement accuracy increases with the increase in the heating strength and duration. In addition, the improved DPHP-DTS method can describe soil evaporation in both sand and loess with good reliability and stability.