Development and psychometric properties of Female Infertility Stigma Instrument (ISI-F): A sequential mixed method study.

BACKGROUND: Infertility stigma is a hidden burden that overshadows the dimensions of reproductive and sexual health in infertile women. The aim of this study was to develop and evaluate the psychometric properties of the Female Infertility Stigma Instrument (ISI-F). METHODS: This mixed method study with sequential exploratory design was conducted in qualitative and quantitative phases. In the first phase, the initial item pool of the Female Infertility Stigma Instrument (ISI-F) was generated using in-depth interviews. In the quantitative phase, psychometric properties of the ISI-f including content, face and construct validity, as well as reliability (internal consistency and stability) were assessed. Exploratory factor analysis was performed on the collected data from 300 infertile women for evaluation of construct validity. Data was analyzed using SPSS version 20. This study has followed the Mixed Methods Article Reporting Standards checklist. RESULTS: The final version of ISI-F had 20 items. Total CVI and CVR were 0.94 and 0.87, respectively. Explanatory factor analysis identified 3 main factors that explained 54.013% of the variance. These factors consisted of stigma profile (7 items), self-stigma (6 items) and escaping from stigma (7 items). Internal consistency and stability of the ISI-F has been approved by Cronbach's alpha, McDonald's Omega (0.909, 0.916) and Intraclass Correlation Coefficient (ICC = 0.878). CONCLUSION: The Female Infertility Stigma Instrument (ISI-F) is a valid and reliable tool for evaluation of the perceived female infertility stigma, that was developed in this study.

Association between infertility factors and perceived relationship quality in infertile couples.

BACKGROUND: Infertility is a crisis in the life of couples that could affect their relationships. The present study was conducted to evaluate the relation between infertility factors and the quality of marital relationship in infertile couples who were referred to the Fertility and Infertility Center of Isfahan. MATERIALS AND METHODS: The present study was conducted cross-sectional on 131 infertile couples who were referred to the Fertility and Infertility Center of Isfahan and had the inclusion criteria and were selected using convenient sampling method. Data gathering tool for the present study was Perceived Relationship Quality Components (PRQC). All the data were analyzed using SPSS software version 21. Analysis was conducted using descriptive and inferential statistics (independent t-test and one-way analysis of variance). The level of significance was set at 0.05 for all the statistical tests. RESULTS: The mean score of PRQC for women and men was 84.05 and 88.46, respectively, and their difference was statistically significant. There was a significant relation between the score of PRQC in women (P = 0.028) and men (P = 0.030) with the infertility factor. Results showed that the mean score of perceived quality of different aspects of the marital relationship was significantly lower when the infertility factor was female (P < 0.05). In women aspects of "satisfaction" (P = 0.024) and "commitment" (P = 0.011) and in men aspect of "love" (P = 0.004) from the perceived aspects of relationship had a significant relation with the infertility factor. CONCLUSIONS: Perceived quality of different aspects of marital relationship had a significant relation with infertility factor in infertile couples. Results of the present study showed that when infertility had female causes, the quality of relationship was more affected. Therefore, in infertility counseling, simultaneous counseling for both spouses with emphasis on women, along with treatments, should be considered.

Dietary Fatty Acid Intakes and the Outcomes of Assisted Reproductive Technique in Infertile Women.

BACKGROUND: The purpose of this study was evaluating the relationship between fatty acid (FA) intakes and the Assisted Reproductive Technique (ART) outcomes in infertile women. METHODS: In this descriptive longitudinal study, a validated food frequency questionnaire (FFQ) was used to measure dietary intakes among 217 women with primary infertility seeking ART treatments at Isfahan Fertility and Infertility Center, Isfahan, Iran. The average number of total and metaphase II (MII) oocytes, the fertilization rate, the ratio of good and bad quality embryo and biochemical and clinical pregnancy were assessed. Analyses were performed using mean, standard deviation, Chi-square test, ANOVA, ANCOVA, logistic regression. RESULTS: A total of 140 women were finally included in the study. There was a positive relationship between the average number of total and MII oocytes and the amount of total fatty acids (TFAs), saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), linoleic acids, linolenic acids, and oleic acids intakes, while eicosapentaenoic acids (EPAs) and docosahexaenoic acids (DHAs) intakes had an inverse relationship. Consuming more amounts of TFAs, SFAs, PUFAs, MUFAs, linoleic acids, and oleic acids was associated with the lower fertilization rate, whereas the consumption of linolenic acids and EPAs increased the fertilization rate. The ratio of good quality embryo was directly affected by the amount of PUFAs intakes. Additionally, there was a negative correlation between the amount of SFAs intakes and the number of pregnant women. CONCLUSION: TFAs, SFA, PUFA, and MUFA intakes could have both beneficial and adverse impacts on ART outcomes.

The Effects of Serum and Follicular Fluid Vitamin D Levels on Assisted Reproductive Techniques: A Prospective Cohort Study.

BACKGROUND: Based on studies on animal models, vitamin D plays an essential role in reproduction by controlling Ca and Mg levels. Despite these findings, the effects of vitamin D deficiency and supplementation on the outcome of assisted reproductive techniques (ART) remain controversial. Therefore, the aim of the present study was to assess the relationship between serum and follicular fluid 25-OH vitamin D levels on reproductive outcomes of infertile women. MATERIALS AND METHODS: This prospective cohort study included 150 infertile women who underwent in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The participants were allocated to one of the three groups according to their serum and follicular fluid 25-OH vitamin D concentrations (less than 10 ng/ml, between 10 and 30 ng/ ml and more than 30 ng/ml), and fertilization, cleavage and biochemical and clinical pregnancy rates were compared among the groups. Data was analyzed by SPSS software and using Chi-square and Spearman correlation coefficient. RESULTS: Serum and follicular fluid vitamin D levels significantly correlated with biochemical (P=0.008), (P=0.003) and clinical pregnancy (P=0.017), (P=0.001) rates respectively . However, the quality of embryos (P=0.125), (P=0.106) and fertilization rate (P=0.082), (P=0.059) were not associated with the level of serum and follicular fluid vitamin D. CONCLUSION: This study found that women with higher levels of vitamin D in their serum and follicular fluid are significantly more likely to achieve pregnancy but without affecting the quality of embryo and fertility rate.