Neurodegenerative effects of air pollutant Particles: Biological mechanisms implicated for Early-Onset Alzheimer's disease.

BACKGROUND: Sporadic Alzheimer's disease (AD) occurs in 99% of all cases and can be influenced by air pollution such as diesel emissions and more recently, an iron oxide particle, magnetite, detected in the brains of AD patients. However, a mechanistic link between air pollutants and AD development remains elusive. AIM: To study the development of AD-relevant pathological effects induced by air pollutant particle exposures and their mechanistic links, in wild-type and AD-predisposed models. METHODS: C57BL/6 (n = 37) and APP/PS1 transgenic (n = 38) mice (age 13 weeks) were exposed to model pollutant iron-based particle (Fe0-Fe3O4, dTEM = 493 ± 133 nm), hydrocarbon-based diesel combustion particle (43 ± 9 nm) and magnetite (Fe3O4, 153 ± 43 nm) particles (66 µg/20 µL/third day) for 4 months, and were assessed for behavioural changes, neuronal cell loss, amyloid-beta (Aß) plaque, immune response and oxidative stress-biomarkers. Neuroblastoma SHSY5Y (differentiated) cells were exposed to the particles (100 µg/ml) for 24 h, with assessments on immune response biomarkers and reactive oxygen species generation. RESULTS: Pollutant particle-exposure led to increased anxiety and stress levels in wild-type mice and short-term memory impairment in AD-prone mice. Neuronal cell loss was shown in the hippocampal and somatosensory cortex, with increased detection of Aß plaque, the latter only in the AD-predisposed mice, with the wild-type not genetically disposed to form the plaque. The particle exposures however, increased AD-relevant immune system responses, including inflammation, in both strains of mice. Exposures also stimulated oxidative stress, although only observed in wild-type mice. The in vitro studies complemented the immune response and oxidative stress observations. CONCLUSIONS: This study provides insights into the mechanistic links between inflammation and oxidative stress to pollutant particle-induced AD pathologies, with magnetite apparently inducing the most pathological effects. No exacerbation of the effects was observed in the AD-predisposed model when compared to the wild-type, indicating a particle-induced neurodegeneration that is independent of disease state.

Fine particle pollution during megafires contains potentially toxic elements.

Wildfires that raged across Australia during the 2019-2020 'Black Summer' produced an enormous quantity of particulate matter (PM) pollution, with plumes that cloaked many urban centres and ecosystems along the eastern seaboard. This has motivated a need to understand the magnitude and nature of PM exposure, so that its impact on both built and natural environments can be more accurately assessed. Here we present the potentially toxic fingerprint of PM captured by building heating, ventilation, and air conditioning filters in Sydney, Australia during the peak of the Wildfires, and from ambient urban emissions one year later (Reference period). Atmospheric PM and meteorological monitoring data were also assessed to determine the magnitude and source of high PM exposure. The wildfires were a major source of PM pollution in Sydney, exceeding the national standards on 19 % of days between November-February. Wildfire particles were finer and more spherical compared to Reference PM, with count median diameters of 892.1 ± 23.1 versus 1484.8 ± 96.7 nm (mean ± standard error). On an equal-mass basis, differences in potentially toxic elements were predominantly due to higher SO42--S (median 20.4 vs 4.7 mg g-1) and NO3--N (2.4 vs 1.2 mg g-1) in Wildfire PM, and higher PO43--P (10.4 vs 1.4 mg g-1) in Reference PM. Concentrations of remaining elements were similar or lower than Reference PM, except for enrichments to F-, Cl-, dissolved Mn, and particulate Mn, Co and Sb. Fractional solubilities of trace elements were similar or lower than Reference PM, except for enhanced Hg (12.1 vs 1.0 %) and greater variability in Cd, Hg and Mn solubility, which displayed upper quartiles exceeding that of Reference PM. These findings contribute to our understanding of human and ecosystem exposures to the toxic components of mixed smoke plumes, especially in regions downwind of the source.

Fuelling phytoremediation: gasoline degradation by green wall systems-a case study.

The capacity for indoor plants including green wall systems to remove specific volatile organic compounds (VOCs) is well documented in the literature; however under realistic settings, indoor occupants are exposed to a complex mixture of harmful compounds sourced from various emission sources. Gasoline vapour is one of the key sources of these emissions, with several studies demonstrating that indoor occupants in areas surrounding gasoline stations or with residentially attached garages are exposed to far higher concentrations of harmful VOCs. Here we assess the potential of a commercial small passive green wall system, commercially named the 'LivePicture Go' from Ambius P/L, Australia, to drawdown VOCs that comprise gasoline vapour, including total VOC (TVOC) removal and specific removal of individual speciated VOCs over time. An 8-h TVOC removal efficiency of 42.45% was achieved, along with the complete removal of eicosane, 1,2,3-trimethyl-benzene, and hexadecane. Further, the green wall also effectively reduced concentrations of a range of harmful benzene derivatives and other VOCs. These results demonstrate the potential of botanical systems to simultaneously remove a wide variety of VOCs, although future research is needed to improve upon and ensure efficiency of these systems over time and within practical applications.

Host transmission dynamics of first- and third-stage Angiostrongylus cantonensis larvae in Bullastra lessoni.

Given the importance of angiostrongyliasis as an emerging infectious disease of humans, companion animals, and wildlife, the current study focused on the transmission dynamics of first- and third-stage larvae of the parasitic nematode, Angiostrongylus cantonensis. The migration of infective larvae and their subsequent distribution within the Lymnaeidae snail, Bullastra lessoni, were investigated over time using microscopic examination of histological sections and fresh tissue. Snails were divided into four anatomical regions: (i) anterior and (ii) posterior cephalopedal masses, (iii) mantle skirt and (iv) visceral mass. The viability of free-swimming third-stage larvae, after their release from snail tissues, was evaluated in vitro by propidium iodide staining and infectivity by in vivo infection of Wistar rats. Snails were sequentially dissected over time to assess the number and anatomical distribution of larvae within each snail and hence infer their migration pathway. Herein, ongoing larval migratory activity was detected over 28 days post-infection. A comparison of infection rates and the larval distribution within the four designated snail regions demonstrated a significant relationship between anatomical region and density of infective larvae, with larvae mostly distributed in the anterior cephalopedal mass (43.6 ± 10.8%) and the mantle skirt (33.0 ± 8.8%). Propidium iodide staining showed that free-swimming third-stage larvae retained viability for between 4 and 8 weeks when stored under laboratory conditions. In contrast to viability, larval infectivity in rats remained for up to 2 weeks only. Knowledge gained from the current work could provide information on the development of new approaches to controlling the transmission of this parasite.

The botanical biofiltration of volatile organic compounds and particulate matter derived from cigarette smoke.

Despite the growing use of control measures, environmental tobacco smoke (ETS) remains a significant pollutant source in indoor air in many areas of the world. Current control methods for reducing ETS exposure are inadequate to protect public health in environments where cigarettes are smoked. An alternative solution is botanical biofiltration which has previously been shown to lower concentrations of volatile organic compounds (VOCs) and particulate matter (PM) from a range of polluted air streams. This study is the first to assess the potential of a botanical biofilter with the species Spathiphyllum wallisii (Peace Lily) for the removal of cigarette-derived VOCs and all size fractions of PM. Single pass removal efficiencies of 43.26% for total VOCs and 34.37% for total suspended particles were achieved. The botanical biofilter reduced the concentrations of a range of harmful ETS chemicals including nicotine, limonene, and toluene. Evaluation of the re-emission of ETS constituents filtered by the botanical biofilter revealed no particle resuspension or off gassing. The results demonstrate the potential of botanical biofilters to reduce public ETS exposure, although further research is needed to improve upon and ensure the efficiency of these systems for practical applications.

Evaluating and comparing the green wall retrofit suitability across major Australian cities.

Urban densification continues to present a unique set of economic and environmental challenges. A growing shortage of green space and infrastructure is intrinsically linked with urban growth and development. With this comes the loss of ecosystem services such as urban heat island effects, reduction of air quality and biodiversity loss. Vertical greenery systems (VGS) offer an adaptive solution to space-constrained areas that are characteristic of dense urban areas, and can potentially improve the sustainability of cities. However, in order to promote VGS uptake, methods are required to enable systematic appraisal of whether existing walls can be retrofitted with VGS. Further, feasibility studies that quantify the potential for retrofit suitability of VGS across entire urban areas are lacking. This study established an evaluation tool for green wall constructability in urban areas and validated the assessment tool by determining the quantity of walls in five major Australian cities that could potentially have VGS incorporated into the existing infrastructure. Each wall was analysed using an exclusionary set of criteria that evaluated and ranked a wall based on its suitability to VGS implementation. Sydney and Brisbane recorded the greatest proportional length of walls suitable for VGS, with 33.74% and 34.12% respectively. Conversely, Perth's urban centre was the least feasible site in which to incorporate VGS, with over 97% of surveyed walls excluded, mainly due to the prevalence of <1 m high fence lines and glazed shopfronts. This study aimed to evaluate feasibility assessments of green wall retrofitability in highly urbanised areas with the intention of creating an analytical method that is accessible to all. This method, coupled with the promising number of feasible walls found in this study, emphasises the need for more government policy and incentives encouraging green wall uptake and could play a pivotal role in the expansion of green infrastructure and urban forestry.

Effective reduction of roadside air pollution with botanical biofiltration.

Currently no sustainable, economical and scalable systems have been developed for the direct removal of roadside air pollutants at their source. Here we present a simple and effective air filtering technology: botanical biofiltration, and the first field assessment of three different botanical biofilter designs for the filtration of traffic associated air pollutants - NO2, O3 and PM2.5 - from roadside ambient air in Sydney, Australia. Over two six month research campaigns, we show that all of the tested systems filtered NO2, O3 and PM2.5 with average single pass removal efficiencies of up to 71.5%, 28.1% and 22.1% respectively. Clean air delivery rates of up to 121 m3/h, 50 m3/h and 40 m3/h per m2 of active green wall biofilter were achieved for the three pollutants respectively, with pollutant removal efficiency positively correlated with their ambient concentrations. We propose that large scale field trials of this technology are warranted to promote sustainable urban development and improved public health outcomes.

Can Green Walls Reduce Outdoor Ambient Particulate Matter, Noise Pollution and Temperature?

Green walls have previously demonstrated the capacity to reduce particulate matter (PM), noise pollution, and temperature conditions in manipulative experiments and computational models. There is, however, minimal evidence that green walls can influence ambient environmental conditions, especially taking into account the variable environmental conditions encountered in situ. The aim of this paper was to determine if green walls have a quantitative effect on ambient air quality in an urban environment. Ambient PM, noise, and temperature were recorded at 12 green wall and adjacent reference wall locations across a dense urban centre, over a 6-month period. The results indicated that PM levels and temperature did not significantly differ between the green wall and reference wall sites. Ambient noise at the green wall sites, however, was significantly lower than at the reference wall locations. It is suggested that mechanically assisted, or 'active' green wall systems may have a higher PM and temperature reduction capacity, and if so, they will be more valuable for installation in situ compared to standard passive systems, although this will require further research.

Airborne particulate matter accumulation on common green wall plants.

In order to better design greening systems for effective particulate matter (PM) removal, it is important to understand the impact leaf traits have on PM deposition. There are however, inconsistences amongst the leaf traits that have previously been correlated with PM accumulation. The aim of this paper was to identify vegetation characteristics of green wall plants that were associated with the accumulation of particulate matter. To determine patterns associated with different leaf morphologies, eleven common ornamental plant species were sampled across 15 sites, over a 6 month duration. PM deposition was determined gravimetrically and its associated size fractions determined microscopically. Linear mixed models were used to identify statistical patterns relating to differences in PM deposition across plant species. PM deposition and the relative frequencies of particle size fractions were found to be statistically different among species, sites and months. Green wall plants were shown to be effective at PM accumulation as all of the assessed plant species had equivalent PM removal efficiency, with minimal evidence of influential leaf characteristics that could enhance PM removal.

Does plant species selection in functional active green walls influence VOC phytoremediation efficiency?

Volatile organic compounds (VOCs) are of public concern due to their adverse health effects. Botanical air filtration is a promising technology for reducing indoor air contaminants, but the underlying mechanisms are not fully understood. This study assessed active botanical biofilters for their single-pass removal efficiency (SPRE) for benzene, ethyl acetate and ambient total volatile organic compounds (TVOCs), at concentrations of in situ relevance. Biofilters containing four plant species (Chlorophytum orchidastrum, Nematanthus glabra, Nephrolepis cordifolia 'duffii' and Schefflera arboricola) were compared to discern whether plant selection influenced VOC SPRE. Amongst all tested plant species, benzene SPREs were between 45.54 and 59.50%, with N. glabra the most efficient. The botanical biofilters removed 32.36-91.19% of ethyl acetate, with C. orchidastrum and S. arboricola recording significantly higher ethyl acetate SPREs than N. glabra and N. cordifolia. These findings thus indicate that plant type influences botanical biofilter VOC removal. It is proposed that ethyl acetate SPREs were dependent on hydrophilic adsorbent sites, with increasing root surface area, root diameter and root mass all associated with increasing ethyl acetate SPRE. The high benzene SPRE of N. glabra is likely due to the high wax content in its leaf cuticles. The SPREs for the relatively low levels of ambient TVOCs were consistent amongst plant species, providing no evidence to suggest that in situ TVOC removal is influenced by plant choice. Nonetheless, as inter-species differences do exist for some VOCs, botanical biofilters using a mixture of plants is proposed.

Determining broad scale associations between air pollutants and urban forestry: A novel multifaceted methodological approach.

Global urbanisation has resulted in population densification, which is associated with increased air pollution, mainly from anthropogenic sources. One of the systems proposed to mitigate urban air pollution is urban forestry. This study quantified the spatial associations between concentrations of CO, NO2, SO2, and PM10 and urban forestry, whilst correcting for anthropogenic sources and sinks, thus explicitly testing the hypothesis that urban forestry is spatially associated with reduced air pollution on a city scale. A Land Use Regression (LUR) model was constructed by combining air pollutant concentrations with environmental variables, such as land cover type and use, to develop predictive models for air pollutant concentrations. Traffic density and industrial air pollutant emissions were added to the model as covariables to permit testing of the main effects after correcting for these air pollutant sources. It was found that the concentrations of all air pollutants were negatively correlated with tree canopy cover and positively correlated with dwelling density, population density and traffic count. The LUR models enabled the establishment of a statistically significant spatial relationship between urban forestry and air pollution mitigation. These findings further demonstrate the spatial relationships between urban forestry and reduced air pollution on a city-wide scale, and could be of value in developing planning policies focused on urban greening.

Active green wall plant health tolerance to diesel smoke exposure.

Poor air quality is an emerging world-wide problem, with most urban air pollutants arising from vehicular emissions. As such, localized high pollution environments, such as traffic tunnels pose a significant health risk. Phytoremediation, including the use of active (ventilated) green walls or botanical biofilters, is gaining recognition as a potentially effective method for air pollution control. Research to date has tested the capacity of these systems to remove low levels of pollutants from indoor environments. If botanical biofilters are to be used in highly polluted environments, the plants used in these systems must be resilient, however, this idea has received minimal research. Thus, testing was conducted to assess the hardiness of the vegetated component of a botanical biofilter to simulated street level air pollutant exposure. A range of morphological, physiological, and biochemical tests were conducted on 8 common green wall plant species prior to and post 5-week exposure to highly concentrated diesel fuel combustion effluent; as a pilot study to investigate viability in in situ conditions. The results indicated that species within the fig family were the most tolerant species of those assessed. It is likely that species within the fig family can withstand enhanced air pollutant conditions, potentially a result of its leaf morphology and physiology. Other species tested were all moderately tolerant to the pollution treatment. We conclude that most common green wall plant species have the capacity to withstand high pollutant environments, however, extended experimentation is needed to rule out potential long term effects along with potential decreases in filter efficiency from accumulative effects on the substrate.

Conservation mycology in Australia and the potential role of citizen science.

Fungi are undoubtedly important for ecosystem functioning; however, they have been omitted or given scant attention in most biodiversity policy documents, management plans, and formal conservation schedules throughout the world. This oversight may be due to a general lack of awareness in the scientific community and compounded by a scarcity of mycology-associated curricula at the tertiary level and a lack of mycologists in research institutions. Although molecular techniques advance the systematic cataloging of fungi and facilitate insights into fungal communities, the scarcity of professional mycologists in the environmental sciences hampers conservation efforts. Conversely, citizen science initiatives are making significant contributions to the mycology discipline by increasing awareness and extending the scope of fungal surveys. Future research by professional and amateur mycologists into the distribution of fungi and their function in ecosystems will help identify wider and more effective conservation goals.

Correspondence Between Urban Bird Roosts and the Presence of Aerosolised Fungal Pathogens.

Habitat fragmentation in urban environments concentrates bird populations that have managed to adapt to these newly developed areas. Consequently, the roosts of these birds are potentially creating environments conducive to fungal growth and dissemination. Airborne fungi derived from these environments are relatively unstudied, as is the potential health risk arising from these fungi. This study documented the diversity of culturable airborne fungal propagules associated with forty urban bird roosts. Environmental variables from each site were recorded to allow us to analyse the correspondence between different bird species, the substrate they occupy and airborne fungal propagules. Associations were established between Rhodotorula and Pacific black ducks, wood ducks, myna birds and miner birds when in the presence of bare soil as a substrate. Further associations were established between Penicillium, Scopulariopsis and Cunninghamella and pigeons, sparrows and swallows living in areas with hard surfaces such as bitumen and rocks.

Reversal of diabetes following transplantation of an insulin-secreting human liver cell line: Melligen cells.

As an alternative to the transplantation of islets, a human liver cell line has been genetically engineered to reverse type 1 diabetes (TID). The initial liver cell line (Huh7ins) commenced secretion of insulin in response to a glucose concentration of 2.5 mmol/l. After transfection of the Huh7ins cells with human islet glucokinase, the resultant Melligen cells secreted insulin in response to glucose within the physiological range; commencing at 4.25 mmol/l. Melligen cells exhibited increased glucokinase enzymatic activity in response to physiological glucose concentrations, as compared with Huh7ins cells. When transplanted into diabetic immunoincompetent mice, Melligen cells restored normoglycemia. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed that both cell lines expressed a range of ß-cell transcription factors and pancreatic hormones. Exposure of Melligen and Huh7ins cells to proinflammatory cytokines (TNF-α, IL-1ß, and IFN-γ) affected neither their viability nor their ability to secrete insulin to glucose. Gene expression (microarray and qRT-PCR) analyses indicated the survival of Melligen cells in the presence of known ß-cell cytotoxins was associated with the expression of NF-κB and antiapoptotic genes (such as BIRC3). This study describes the successful generation of an artificial ß-cell line, which, if encapsulated to avoid allograft rejection, may offer a clinically applicable cure for T1D.

Supplementation with carnosine decreases plasma triglycerides and modulates atherosclerotic plaque composition in diabetic apo E(-/-) mice.

OBJECTIVE: Carnosine has been shown to modulate triglyceride and glycation levels in cell and animal systems. In this study we investigated whether prolonged supplementation with carnosine inhibits atherosclerosis and markers of lesion stability in hyperglycaemic and hyperlipidaemic mice. METHODS: Streptozotocin-induced diabetic apo E(-/-) mice were maintained for 20 weeks, post-induction of diabetes. Half of the animals received carnosine (2g/L) in their drinking water. Diabetes was confirmed by significant increases in blood glucose and glycated haemoglobin, plasma triglyceride and total cholesterol levels, brachiocephalic artery and aortic sinus plaque area; and lower body mass. RESULTS: Prolonged carnosine supplementation resulted in a significant (∼20-fold) increase in plasma carnosine levels, and a significant (∼23%) lowering of triglyceride levels in the carnosine-supplemented groups regardless of glycaemic status. Supplementation did not affect glycaemic status, blood cholesterol levels or loss of body mass. In the diabetic mice, carnosine supplementation did not diminish measured plaque area, but reduced the area of plaque occupied by extracellular lipid (∼60%) and increased both macrophage numbers (∼70%) and plaque collagen content (∼50%). The area occupied by α-actin-positive smooth muscle cells was not significantly increased. CONCLUSIONS: These data indicate that in a well-established model of diabetes-associated atherosclerosis, prolonged carnosine supplementation enhances plasma levels, and has novel and significant effects on atherosclerotic lesion lipid, collagen and macrophage levels. These data are consistent with greater lesion stability, a key goal in treatment of existing cardiovascular disease. Carnosine supplementation may therefore be of benefit in lowering triglyceride levels and suppressing plaque instability in diabetes-associated atherosclerosis.

Association of SLC11A1 promoter polymorphisms with the incidence of autoimmune and inflammatory diseases: a meta-analysis.

Solute carrier family 11 member a1 (SLC11A1) exerts pleiotropic effects on macrophage function. Expression of SLC11A1 is regulated by a (GT)(n) microsatellite promoter repeat polymorphism of which nine alleles have been described. Enhanced activation of macrophages, associated with increased expression from allele 3, may be functionally linked to the development of autoimmune and inflammatory diseases. Conversely, low expression, driven by allele 2, may afford resistance. We have performed a meta-analysis to determine the association of SLC11A1 promoter alleles 2 and 3 with autoimmunity and inflammation. A random effects pooled odds ratio (OR) of 1.04 (95% confidence interval [CI]=0.20) for allele 3 suggested a weak association of this allele with an increased risk of disease. Calculation of the OR in the absence of asymmetry yielded a random effects pooled OR of 0.88 (95% CI=0.66), effectively reversing the above association. A fixed effects pooled OR of 0.90 (95% CI=0.24) was obtained for allele 2, suggesting a weak predominance of disease in the absence of this allele. Application of the trim-and-fill method resulted in a fixed effects OR of 0.80 (95% CI=0.22), thus strengthening this association. Associations of allele 3 with autoimmune and inflammatory diseases reported in several association studies may be attributable to some form of bias amongst published results.