RÉSUMÉ
The PIWI-interacting RNA (piRNA) pathway is an adaptive defense system wherein piRNAs guide PIWI family Argonaute proteins to recognize and silence ever-evolving selfish genetic elements and ensure genome integrity. Driven by this intensive host-pathogen arms race, the piRNA pathway and its targeted transposons have coevolved rapidly in a species-specific manner, but how the piRNA pathway adapts specifically to target silencing in mammals remains elusive. Here, we show that mouse MILI and human HILI piRNA-induced silencing complexes (piRISCs) bind and cleave targets more efficiently than their invertebrate counterparts from the sponge Ephydatia fluviatilis. The inherent functional differences comport with structural features identified by cryo-EM studies of piRISCs. In the absence of target, MILI and HILI piRISCs adopt a wider nucleic-acid-binding channel and display an extended prearranged piRNA seed as compared with EfPiwi piRISC, consistent with their ability to capture targets more efficiently than EfPiwi piRISC. In the presence of target, the seed gate-which enforces seed-target fidelity in microRNA RISC-adopts a relaxed state in mammalian piRISC, revealing how MILI and HILI tolerate seed-target mismatches to broaden the target spectrum. A vertebrate-specific lysine distorts the piRNA seed, shifting the trajectory of the piRNA-target duplex out of the central cleft and toward the PAZ lobe. Functional analyses reveal that this lysine promotes target binding and cleavage. Our study therefore provides a molecular basis for the piRNA targeting mechanism in mice and humans, and suggests that mammalian piRNA machinery can achieve broad target silencing using a limited supply of piRNA species.
Sujet(s)
Protéines Argonaute , ARN interagissant avec Piwi , Animaux , Humains , Souris , Protéines Argonaute/métabolisme , Protéines Argonaute/génétique , Protéines Argonaute/composition chimique , Cryomicroscopie électronique , Extinction de l'expression des gènes , ARN interagissant avec Piwi/génétique , ARN interagissant avec Piwi/métabolismeRÉSUMÉ
Retrosynthetic planning, which aims to identify synthetic pathways for target molecules from starting materials, is a fundamental problem in synthetic chemistry. Computer-aided retrosynthesis has made significant progress, in which heuristic search algorithms, including Monte Carlo Tree Search (MCTS) and A* search, have played a crucial role. However, unreliable guiding heuristics often cause search failure due to insufficient exploration. Conversely, excessive exploration also prevents the search from reaching the optimal solution. In this paper, MCTS exploration enhanced A* (MEEA*) search is proposed to incorporate the exploratory behavior of MCTS into A* by providing a look-ahead search. Path consistency is adopted as a regularization to improve the generalization performance of heuristics. Extensive experimental results on 10 molecule datasets demonstrate the effectiveness of MEEA*. Especially, on the widely used United States Patent and Trademark Office (USPTO) benchmark, MEEA* achieves a 100.0% success rate. Moreover, for natural products, MEEA* successfully identifies bio-retrosynthetic pathways for 97.68% test compounds.
RÉSUMÉ
Designing 3D molecules with high binding affinity for specific protein targets is crucial in drug design. One challenge is that the atomic interaction between molecules and proteins in 3D space has to be taken into account. However, the existing target-aware methods solely model the joint distribution between the molecules and proteins, disregarding the binding affinities between them, which leads to limited performance. In this paper, we propose an explainable diffusion model to generate molecules that can be bound to a given protein target with high affinity. Our method explicitly incorporates the chemical knowledge of protein-ligand binding affinity into the diffusion model, and uses the knowledge to guide the denoising process towards the direction of high binding affinity. Specifically, an SE(3)-invariant expert network is developed to fit the Vina scoring functions and jointly trained with the denoising network, while the domain knowledge is distilled and conveyed from Vina functions to the expert network. An effective guidance is proposed on both continuous atom coordinates and discrete atom types by taking advantages of the gradient of the expert network. Experiments on the benchmark CrossDocked2020 demonstrate the superiority of our method. Additionally, an atom-level explanation of the generated molecules is provided, and the connections with the domain knowledge are established.
Sujet(s)
Conception de médicament , Protéines , Protéines/composition chimique , Liaison aux protéines , LigandsRÉSUMÉ
De novo molecular generation is a promising approach to drug discovery, building novel molecules from the scratch that can bind the target proteins specifically. With the increasing availability of machine learning algorithms and computational power, artificial intelligence (AI) has emerged as a valuable tool for this purpose. Here, we have developed a database of 3D ligands that collects six AI models for de novo molecular generation based on target proteins, including 20 disease-associated targets. Our database currently includes 1767 protein targets and up to 164 107 de novo-designed molecules. The primary goal is to provide an easily accessible and user-friendly molecular database for professionals in the fields of bioinformatics, pharmacology and related areas, enabling them to efficiently screen for potential lead compounds with biological activity. Additionally, our database provides a comprehensive resource for computational scientists to explore and compare different AI models in terms of their performance in generating novel molecules with desirable properties. All the resources and services are publicly accessible at https://cmach.sjtu.edu.cn/drug/. Database URL: https://cmach.sjtu.edu.cn/drug/.
Sujet(s)
Intelligence artificielle , Protéines , Algorithmes , Découverte de médicament , Bases de données factuelles , Conception de médicamentRÉSUMÉ
Encoding sketches as Gaussian mixture model (GMM)-distributed latent codes is an effective way to control sketch synthesis. Each Gaussian component represents a specific sketch pattern, and a code randomly sampled from the Gaussian can be decoded to synthesize a sketch with the target pattern. However, existing methods treat the Gaussians as individual clusters, which neglects the relationships between them. For example, the giraffe and horse sketches heading left are related to each other by their face orientation. The relationships between sketch patterns are important messages to reveal cognitive knowledge in sketch data. Thus, it is promising to learn accurate sketch representations by modeling the pattern relationships into a latent structure. In this article, we construct a tree-structured taxonomic hierarchy over the clusters of sketch codes. The clusters with the more specific descriptions of sketch patterns are placed at the lower levels, while the ones with the more general patterns are ranked at the higher levels. The clusters at the same rank relate to each other through the inheritance of features from common ancestors. We propose a hierarchical expectation-maximization (EM)-like algorithm to explicitly learn the hierarchy, jointly with the training of encoder-decoder network. Moreover, the learned latent hierarchy is utilized to regularize sketch codes with structural constraints. Experimental results show that our method significantly improves controllable synthesis performance and obtains effective sketch analogy results.
RÉSUMÉ
It is critical to accurately predict the rupture risk of an intracranial aneurysm (IA) for timely and appropriate treatment because the fatality rate after rupture is 50 % . Existing methods relying on morphological features (e.g., height-width ratio) measured manually by neuroradiologists are labor intensive and have limited use for risk assessment. Therefore, we propose an end-to-end deep-learning method, called TransIAR net, to automatically learn the morphological features from 3D computed tomography angiography (CTA) data and accurately predict the status of IA rupture. We devise a multiscale 3D convolutional neural network (CNN) to extract the structural patterns of the IA and its neighborhood with a dual branch of shared network structures. Moreover, we learn the spatial dependence within the IA neighborhood with a transformer encoder. Our experiments demonstrated that the features learned by TransIAR are more effective and robust than handcrafted features, resulting in a 10 % - 15 % improvement in the accuracy of rupture status prediction.
RÉSUMÉ
Protein binding site prediction is an important prerequisite task of drug discovery and design. While binding sites are very small, irregular and varied in shape, making the prediction very challenging. Standard 3D U-Net has been adopted to predict binding sites but got stuck with unsatisfactory prediction results, incomplete, out-of-bounds, or even failed. The reason is that this scheme is less capable of extracting the chemical interactions of the entire region and hardly takes into account the difficulty of segmenting complex shapes. In this paper, we propose a refined U-Net architecture, called RefinePocket, consisting of an attention-enhanced encoder and a mask-guided decoder. During encoding, taking binding site proposal as input, we employ Dual Attention Block (DAB) hierarchically to capture rich global information, exploring residue relationship and chemical correlations in spatial and channel dimensions respectively. Then, based on the enhanced representation extracted by the encoder, we devise Refine Block (RB) in the decoder to enable self-guided refinement of uncertain regions gradually, resulting in more precise segmentation. Experiments show that DAB and RB complement and promote each other, making RefinePocket has an average improvement of 10.02% on DCC and 4.26% on DVO compared with the state-of-the-art method on four test sets.
Sujet(s)
Apprentissage profond , Liaison aux protéines , Sites de fixation , Découverte de médicament , Domaines protéiques , Traitement d'image par ordinateurRÉSUMÉ
Accurate prediction of synergistic effects of drug combinations can reduce the experimental costs for drug development and facilitate the discovery of novel efficacious combination therapies for clinical studies. The drug combinations with high synergy scores are regarded as synergistic ones, while those with moderate or low synergy scores are additive or antagonistic ones. The existing methods usually exploit the synergy data from the aspect of synergistic drug combinations, paying little attention to the additive or antagonistic ones. Also, they usually do not leverage the common patterns of drug combinations across different cell lines. In this paper, we propose a multi-channel graph autoencoder (MGAE)-based method for predicting the synergistic effects of drug combinations (DC), and shortly denote it as MGAE-DC. A MGAE model is built to learn the drug embeddings by considering not only synergistic combinations but also additive and antagonistic ones as three input channels. The later two channels guide the model to explicitly characterize the features of non-synergistic combinations through an encoder-decoder learning process, and thus the drug embeddings become more discriminative between synergistic and non-synergistic combinations. In addition, an attention mechanism is incorporated to fuse each cell-line's drug embeddings across various cell lines, and a common drug embedding is extracted to capture the invariant patterns by developing a set of cell-line shared decoders. The generalization performance of our model is further improved with the invariant patterns. With the cell-line specific and common drug embeddings, our method is extended to predict the synergy scores of drug combinations by a neural network module. Experiments on four benchmark datasets demonstrate that MGAE-DC consistently outperforms the state-of-the-art methods. In-depth literature survey is conducted to find that many drug combinations predicted by MGAE-DC are supported by previous experimental studies. The source code and data are available at https://github.com/yushenshashen/MGAE-DC.
Sujet(s)
Référenciation , Développement de médicament , Lignée cellulaire , Association médicamenteuse ,RÉSUMÉ
Semantic face editing has achieved substantial progress in recent years. However, existing face editing methods, which often encode the entire image into a single code, still have difficulty in enabling flexible editing while keeping high-fidelity reconstruction. The one-code scheme also brings entangled face manipulations and limited flexibility in editing face components. In this paper, we present IA-FaceS, a bidirectional method for disentangled face attribute manipulation as well as flexible, controllable component editing. We propose to embed images onto two branches: one branch computes high-dimensional component-invariant content embedding for capturing face details, and the other provides low-dimensional component-specific embeddings for component manipulations. The two-branch scheme naturally enables high-quality facial component-level editing while keeping faithful reconstruction with details. Moreover, we devise a component adaptive modulation (CAM) module, which integrates component-specific guidance into the decoder and successfully disentangles highly-correlated face components. The single-eye editing is developed for the first time without editing face masks or sketches. According to the experimental results, IA-FaceS establishes a good balance between maintaining image details and performing flexible face manipulation. Both quantitative and qualitative results indicate that the proposed method outperforms the existing methods in reconstruction, face attribute manipulation, and component transfer. We release the code and weights at: https://github.com/CMACH508/IA-FaceS.
Sujet(s)
Traitement d'image par ordinateur , Sémantique , Traitement d'image par ordinateur/méthodesRÉSUMÉ
piRNAs function as guardians of the genome by silencing non-self nucleic acids and transposable elements in animals. Many piRNA factors are enriched in perinuclear germ granules, but whether their localization is required for piRNA biogenesis or function is not known. Here we show that GLH/VASA helicase mutants exhibit defects in forming perinuclear condensates containing PIWI and other small RNA cofactors. These mutant animals produce largely normal levels of piRNA but are defective in triggering piRNA silencing. Strikingly, while many piRNA targets are activated in GLH mutants, we observe that hundreds of endogenous genes are aberrantly silenced by piRNAs. This defect in self versus non-self recognition is also observed in other mutants where perinuclear germ granules are disrupted. Together, our results argue that perinuclear germ granules function critically to promote the fidelity of piRNA-based transcriptome surveillance in C. elegans and preserve self versus non-self distinction.
Sujet(s)
Caenorhabditis elegans , Transcriptome , Animaux , Caenorhabditis elegans/génétique , Helicase/génétique , Granules de ribonucléoprotéines des cellules germinales , Cellules germinales , Petit ARN interférent/génétique , Transcriptome/génétiqueRÉSUMÉ
Identifying synergistic drug combinations (SDCs) is a great challenge due to the combinatorial complexity and the fact that SDC is cell line specific. The existing computational methods either did not consider the cell line specificity of SDC, or did not perform well by building model for each cell line independently. In this paper, we present a novel encoder-decoder network named SDCNet for predicting cell line-specific SDCs. SDCNet learns common patterns across different cell lines as well as cell line-specific features in one model for drug combinations. This is realized by considering the SDC graphs of different cell lines as a relational graph, and constructing a relational graph convolutional network (R-GCN) as the encoder to learn and fuse the deep representations of drugs for different cell lines. An attention mechanism is devised to integrate the drug features from different layers of the R-GCN according to their relative importance so that representation learning is further enhanced. The common patterns are exploited through partial parameter sharing in cell line-specific decoders, which not only reconstruct the known SDCs but also predict new ones for each cell line. Experiments on various datasets demonstrate that SDCNet is superior to state-of-the-art methods and is also robust when generalized to new cell lines that are different from the training ones. Finally, the case study again confirms the effectiveness of our method in predicting novel reliable cell line-specific SDCs.
Sujet(s)
, Association médicamenteuse , Lignée cellulaireRÉSUMÉ
Current face recognition tasks are usually carried out on high-quality face images, but in reality, most face images are captured under unconstrained or poor conditions, e.g., by video surveillance. Existing methods are featured by learning data uncertainty to avoid overfitting the noise, or by adding margins to the angle or cosine space of the normalized softmax loss to penalize the target logit, which enforces intra-class compactness and inter-class discrepancy. In this paper, we propose a deep Rival Penalized Competitive Learning (RPCL) for deep face recognition in low-resolution (LR) images. Inspired by the idea of the RPCL, our method further enforces regulation on the rival logit, which is defined as the largest non-target logit for an input image. Different from existing methods that only consider penalization on the target logit, our method not only strengthens the learning towards the target label, but also enforces a reverse direction, i.e., becoming de-learning, away from the rival label. Comprehensive experiments demonstrate that our method improves the existing state-of-the-art methods to be very robust for LR face recognition.
Sujet(s)
Algorithmes , Reconnaissance faciale , Face , ApprentissageRÉSUMÉ
Traditional drug discovery is very laborious, expensive, and time-consuming, due to the huge combinatorial complexity of the discrete molecular search space. Researchers have turned to machine learning methods for help to tackle this difficult problem. However, most existing methods are either virtual screening on the available database of compounds by protein-ligand affinity prediction, or unconditional molecular generation, which does not take into account the information of the protein target. In this paper, we propose a protein target-oriented de novo drug design method, called AlphaDrug. Our method is able to automatically generate molecular drug candidates in an autoregressive way, and the drug candidates can dock into the given target protein well. To fulfill this goal, we devise a modified transformer network for the joint embedding of protein target and the molecule, and a Monte Carlo tree search (MCTS) algorithm for the conditional molecular generation. In the transformer variant, we impose a hierarchy of skip connections from protein encoder to molecule decoder for efficient feature transfer. The transformer variant computes the probabilities of next atoms based on the protein target and the molecule intermediate. We use the probabilities to guide the look-ahead search by MCTS to enhance or correct the next-atom selection. Moreover, MCTS is also guided by a value function implemented by a docking program, such that the paths with many low docking values are seldom chosen. Experiments on diverse protein targets demonstrate the effectiveness of our methods, indicating that AlphaDrug is a potentially promising solution to target-specific de novo drug design.
RÉSUMÉ
Learning to synthesize free-hand sketches controllably according to specified categories and sketching styles is a challenging task, due to the lack of training data with category labels and style labels. One choice to control the synthesis is by self-organizing a latent coding space to preserve the similarity of structural patterns of the observed data. A practical way is introducing a Gaussian mixture prior over the latent codes, where each Gaussian component represents a specific categorical or stylistic pattern. As a result, we can generate sketches by sampling the latent variables from the Gaussian components or continuously manipulating the latent representations by interpolation. To achieve robust controllable sketch synthesis, it is critical to determine an appropriate Gaussian number. An underestimated Gaussian number cannot fully represent all the sketch patterns, i.e., some clusters have to contain sketches with more than one pattern. An overestimated one introduces redundant components, usually representing a chaotic collection of sketches with diverse patterns featured by other components. Both cases disturb pattern clustering over the coding space and make the internal code generation difficult to control for specific patterns. However, the Gaussian number is unavailable in this unsupervised task. In this paper, we present Rival Penalized Competitive Learning pixel to sequence (RPCL-pix2seq) to automatically determine the Gaussian number. Both quantitative and qualitative experimental results show RPCL-pix2seq can partition the codes for the sketches into an approximate stable number of clusters. Hence, we are able to do synthesis reasoning over the latent space, generating novel but reasonable sketches which neither appear in the training dataset nor exist in real life.
Sujet(s)
Apprentissage machine , Reconnaissance automatique des formes/méthodesRÉSUMÉ
The coronavirus disease 2019 (COVID-19) epidemic continues to spread rapidly around the world and nearly 20 millions people are infected. This paper utilises both single-locus analysis and joint-SNPs analysis for detection of significant single nucleotide polymorphisms (SNPs) in the phenotypes of symptomatic versus asymptomatic, the early collection time versus the late collection time, the old versus the young, and the male versus the female. Also, this paper analyses the relationship between any two SNPs via linkage disequilibrium analysis, and visualises the patterns of cumulative mutations of SNPs over collection time. The results are in three folds. First, the SNP which locates at the nucleotide position 4321 is found to be an independent significant locus associated with all the first three phenotypes. Moreover, 12 significant SNPs are found in the first two studies. Second, gene orf1ab containing SNP-4321 is detected to be significantly associated with the first three phenotypes, and the three genes S, ORF3a, and N, are detected to be significant in the first two phenotypes. Third, some of the detected genes or SNPs are related to the SARS-COV-2 as supported by literature survey, which indicates that the results here may be helpful for further investigation.
Sujet(s)
COVID-19/virologie , Génome viral , Mutation , SARS-CoV-2/génétique , COVID-19/épidémiologie , Biologie informatique , Bases de données génétiques , Femelle , Étude d'association pangénomique , Humains , Japon/épidémiologie , Déséquilibre de liaison , Mâle , Adulte d'âge moyen , Pandémies , Phénotype , Polymorphisme de nucléotide simple , Séquençage du génome entierRÉSUMÉ
Aim: To analyze the m6A methylome of osteosarcoma stem cells (OSCs). Materials & methods: Chemoresistant OSCs were enriched by doxorubicin treatment. Expression of m6A-related enzymes was detected by quantitative real-time-PCR and western blot. MeRIP-seq and RNA-seq were performed to identify differences in m6A methylation and gene expression. Data analysis was conducted to explore the modified genes and their clinical significance. Results: Three m6A-related enzymes were altered in OSCs. Differentially methylated genes were enriched in some pathways regulating pluripotency of stem cells. The expression of several candidate genes were found consistent with that in GSE33458 dataset, and associated with poor prognosis in osteosarcoma patients. Conclusion: m6A may play a role in the emergence and maintaining of OSCs and affect the prognosis.
Sujet(s)
Adénosine/génétique , Tumeurs osseuses/génétique , Ostéosarcome/génétique , Transcriptome/génétique , Tumeurs osseuses/anatomopathologie , Lignée cellulaire tumorale , Épigenèse génétique/génétique , Épigénome/génétique , Analyse de profil d'expression de gènes , Humains , Méthylation , Cellules souches tumorales/anatomopathologie , Ostéosarcome/anatomopathologie , ARN/génétiqueRÉSUMÉ
PIWI-interacting RNAs (piRNAs) are a class of small noncoding RNAs that guard animal genomes against mutation by silencing transposons. In addition, recent studies have reported that piRNAs silence various endogenous genes. Tens of thousands of distinct piRNAs made in animals do not pair well to transposons and currently the functions and targets of piRNAs are largely unexplored. piRTarBase provides a user-friendly interface to access both predicted and experimentally identified piRNA targeting sites in Caenorhabditis elegans. The user can input genes of interest and retrieve a list of piRNA targeting sites on the input genes. Alternatively, the user can input a piRNA and retrieve a list of its mRNA targets. Additionally, piRTarBase integrates published mRNA and small RNA sequencing data, which will help users identify biologically relevant targeting events. Importantly, our analyses suggest that the piRNA sites found by both predictive and experimental approaches are more likely to exhibit silencing effects on their targets than each method alone. Taken together, piRTarBase offers an integrative platform that will help users to identify functional piRNA target sites by evaluating various information. piRTarBase is freely available for academic use at http://cosbi6.ee.ncku.edu.tw/piRTarBase/.
Sujet(s)
Sites de fixation , Bases de données génétiques , Régulation de l'expression des gènes , Extinction de l'expression des gènes , Interférence par ARN , ARN messager/génétique , Petit ARN interférent/génétique , Logiciel , Navigateur , Flux de travauxRÉSUMÉ
Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
Sujet(s)
Protéines de Caenorhabditis elegans/métabolisme , Caenorhabditis elegans/métabolisme , Protéines de transport/métabolisme , Cellules germinales/métabolisme , Interférence par ARN , Animaux , Chromatine/métabolisme , Réseaux de régulation géniqueRÉSUMÉ
In Drosophila, the piRNAs that guide germline transposon silencing are produced from heterochromatic clusters marked by the HP1 homolog Rhino. We show that Rhino promotes cluster transcript association with UAP56 and the THO complex, forming RNA-protein assemblies that are unique to piRNA precursors. UAP56 and THO are ubiquitous RNA-processing factors, and null alleles of uap56 and the THO subunit gene tho2 are lethal. However, uap56sz15 and mutations in the THO subunit genes thoc5 and thoc7 are viable but sterile and disrupt piRNA biogenesis. The uap56sz15 allele reduces UAP56 binding to THO, and the thoc5 and thoc7 mutations disrupt interactions among the remaining THO subunits and UAP56 binding to the core THO subunit Hpr1. These mutations also reduce Rhino binding to clusters and trigger Rhino binding to ectopic sites across the genome. Rhino thus promotes assembly of piRNA precursor complexes, and these complexes restrict Rhino at cluster heterochromatin.
Sujet(s)
Hétérochromatine/métabolisme , Petit ARN interférent/métabolisme , Animaux , Sites de fixation , Protéines chromosomiques nonhistones/métabolisme , DEAD-box RNA helicases/métabolisme , Protéines de Drosophila/métabolisme , Drosophila melanogaster , Hétérochromatine/génétique , Protéines nucléaires/métabolisme , Liaison aux protéines , Petit ARN interférent/génétiqueRÉSUMÉ
pirScan is a web-based tool for identifying C. elegans piRNA-targeting sites within a given mRNA or spliced DNA sequence. The purpose of our tool is to allow C. elegans researchers to predict piRNA targeting sites and to avoid the persistent germline silencing of transgenes that has rendered many constructs unusable. pirScan fulfills this purpose by first enumerating the predicted piRNA-targeting sites present in an input sequence. This prediction can be exported in a tabular or graphical format. Subsequently, pirScan suggests silent mutations that can be introduced to the input sequence that would allow the modified transgene to avoid piRNA targeting. The user can customize the piRNA targeting stringency and the silent mutations that he/she wants to introduce into the sequence. The modified sequences can be re-submitted to be certain that any previously present piRNA-targeting sites are now absent and no new piRNA-targeting sites are accidentally generated. This revised sequence can finally be downloaded as a text file and/or visualized in a graphical format. pirScan is freely available for academic use at http://cosbi4.ee.ncku.edu.tw/pirScan/.