Combination of preconditioned adipose-derived mesenchymal stem cells and platelet-rich plasma improves the repair of osteoarthritis in rat.

Aim: To observe the combined effect of platelet-rich plasma (PRP) and preconditioned adipose-derived mesenchymal stem cells (ADMSCs) on the injured articular cartilage of the rat. Materials & methods: Animals in the study received an intra-articular injection of PRP and preconditioned ADMSCs, both in combination and separately. The response to therapeutic intervention was evaluated by inflammatory markers, proteoglycans content, chondrogenesis and gene expression analyses. Results: The combined therapy resulted in a reduction of IL-6 and TNF-α, increased proteoglycan content of the articular cartilage, upregulation of Acan, Col2a1 and PCNA genes. Downregulation of Col1a1, Col10a1 and Casp3 genes was observed as compared with the untreated osteoarthritis rat model. Conclusion: PRP potentiates the effects of ADMSCs on the repair of damaged articular cartilage.

Analytical study of biosynthesised silver nanoparticles against multi-drug resistant biofilm-forming pathogens.

The emergence of the huge number of multi-drug resistant (MDR) bacteria requires an alternative to the drugs. Silver nanoparticles (AgNPs) are a strong candidate for this due to their bactericidal properties, which can be better concluded by understanding their morphology and chemistry. The study hypothesised that AgNPs synthesised using leaves of Syzygium cumini can be used to treat locally emerging MDRs forming biofilms on indwelling medical devices. Synthesised particles were characterised by methods like UV-visible spectroscopy, X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, and Zetasizer. Fourier transform infrared spectroscopy, and high-performance liquid chromatography were used to predict phytochemicals present in the leaves. The shape of particles is revealed to be relatively spherical, with average size to be around 10-100 nm. Phenolic compounds are attributed to the formation of nanoparticles, stability analysis shows particles to be stable, and zeta potential determined the surface charge to be -20.1 mV. Biosynthesised particles are found to possess efficient antibacterial activity MDR bacteria developing biofilms in medical devices; hence, it is concluded that S. cumini based NPs can be used to develop a layer on implant-related medical devices. Toxicity evaluation against A594 cancer cells portrays AgNPs to be potential tumour reduction agents in a concentration-dependent manner.