RÉSUMÉ
It has been previously demonstrated that the sea star axial organ is a primitive immune organ. Phagocytic, lymphoid-like cells have been characterized with properties similar to those of vertebrates. There is also evidence for an invertebrate cytokine network because IL-1 and TNF-like activities are clearly demonstrable. In addition, the authors have previously described preliminary evidence for IL-2-like activity in the sea star. In the present report, the authors obtained evidence for the presence of IL-1- and IL-2-like molecules on axial organ cells. More interestingly, the results suggested that sea star cells express structures similar to human receptors for IL-1, IL-2, IL-6 and IFN-gamma.
Sujet(s)
Cytokines/analyse , Tissu lymphoïde/composition chimique , Récepteurs aux cytokines/analyse , Étoile de mer/métabolisme , Animaux , Anticorps monoclonaux , Hémocyanine/pharmacologie , Tissu lymphoïde/effets des médicaments et des substances chimiques , Tissu lymphoïde/immunologie , Phénotype , Étoile de mer/immunologie , Activation chimiqueRÉSUMÉ
The axial organ of sea star Asterias rubens is a primitive immune organ. The total cell population was fractionated into two populations: adherent (B-like) and non-adherent cells (T-like) to nylon wool. These two cell subsets were previously defined as functionally acting as mammals T and B cells. In the present report, we pointed out that these T and B-like cells can be identified using mouse to human monoclonal antibodies. Reproducible results were obtained with anti-CD7 monoclonal antibody which detects the T-like cell subset and with anti-CD14 antibody that characterizes the B-like population.
Sujet(s)
Sous-populations de lymphocytes B/immunologie , Étoile de mer/immunologie , Sous-populations de lymphocytes T/immunologie , Animaux , Anticorps monoclonaux/immunologie , Réactions croisées , Cytométrie en flux , Humains , Étoile de mer/cytologieRÉSUMÉ
We report on 2 patients with isochromosome 21q [i(21q)] or translocation 21q21q [t(21q21q)] in myeloid disorders. Of 18 available cases of i(21q) or t(21q21q), 15 were found in myeloid malignancies, often secondary to a previous carcinogen exposure. Complex karyotypes were found in most cases. Four cases presented with i(21q) or t(21q21q) as the sole anomaly, and this might represent a specific entity.