RÉSUMÉ
In experiment on rats in the immunodeficiency the use of recombinant epidermal growth factor was substantiated that let to speed up reparative regeneration at concentration 500 ppb/l drinking water.
Sujet(s)
Facteur de croissance épidermique/usage thérapeutique , Bouche/effets des médicaments et des substances chimiques , Bouche/traumatismes , Protéines recombinantes/usage thérapeutique , Cicatrisation de plaie/effets des médicaments et des substances chimiques , Animaux , Déficit immunitaire commun variable , Muqueuse/effets des médicaments et des substances chimiques , Muqueuse/traumatismes , Rats , Rat WistarRÉSUMÉ
In experiment on 12 Chinchilla rabbits dynamics of reparative regeneration was studied at the terms 2 and 4 months. Bone defect in mandible corner was closed by osteoplastic material Gapkol which was covered from inside by allogenic or autologic stem cells received from rabbit adipose tissue. The results of the ray tracing methods of study were verified by SEM and histological methods.
Sujet(s)
Régénération osseuse , Collagène , Matériaux dentaires , Durapatite , Mandibule/chirurgie , Transplantation de cellules souches mésenchymateuses , Ostéogenèse , Tissu adipeux , Animaux , Association médicamenteuse , Études de suivi , Cellules souches mésenchymateuses/cytologie , Microscopie électronique à balayage , Ostéosclérose/chirurgie , Lapins , Facteurs temps , Tomodensitométrie , Transplantation autologue , Transplantation homologueRÉSUMÉ
Reconstructive surgery often collides with complicated task of defect recovery in the situation of deficit of maxillofacial skeleton tissues, that leads to necessary search of accessible cells source with osteogenic potentials and proper substrate for cell transplantation. In the study the regeneratory potential of stromal cells of adipose tissue was tested in the rabbit model of mandible through angle defect. Cells of allogenic and autologic nature stimulated reparative osteogenesis but their influence upon bone matrix maturation was different. When the construction with allogenic cells was transplanted reaction of its rejection was not detected on histological level.
Sujet(s)
Tissu adipeux/cytologie , Régénération osseuse , Mandibule/chirurgie , Cellules souches multipotentes , Ostéogenèse , Transplantation de cellules souches , Cellules stromales , Animaux , Trame osseuse/physiologie , Différenciation cellulaire , Études de suivi , Mandibule/physiologie , Cellules souches multipotentes/physiologie , Cellules souches multipotentes/transplantation , Ostéogenèse/physiologie , Lapins , Cellules stromales/physiologie , Cellules stromales/transplantation , Facteurs temps , Ingénierie tissulaire , Transplantation autologue , Transplantation homologueRÉSUMÉ
In experiment on 16 grown-up chinchilla rabbits the dynamic of reparative regeneration was evaluated by digital microfocal rontgenography in the terms of 1, 2 and 4 months. Bone defect of the 8capital CHE, Cyrillic8 mm size in the region of mandible angle was caused by surgical laser Smart 2940 D+ on the right side and by physiodespenser Surgec XT on the left side. Surgical laser use let to reduce intact mother bone traumatisation and to improve remote results of bone tissue regeneration. After bone defect creation bone tissue regeneration was put into effect by all 3 callus types - endosteal, periosteal and intermediary.
Sujet(s)
Régénération osseuse , Fractures mandibulaires , Tomodensitométrie/méthodes , Animaux , Cal osseux , Chinchilla , Études de suivi , Lasers , Fractures mandibulaires/imagerie diagnostique , Fractures mandibulaires/étiologie , Facteurs tempsRÉSUMÉ
In experiment on rabbits by histomorphometric method it was shown that after the use during 4 months of titanium plate with the culture of marrow mesenchymal stromal cells there happened full infill of mandible defect by lamellar bone tissue. In control group (titanium plate with inactivated culture of cells) the defect was replaced by regenerate, the most part of it was rough fibrous connective tissue. In experimental group in the central parts of the defect regenerate were characterized by bigger weight of lamellar bone matrix if compared with the peripheral parts of the defect that testified to higher level of its differentiation. Use of stem cells on titanium carrier led in 4 months experiment to full infill of jaw defect by lamellar bone tissue.
Sujet(s)
Régénération osseuse , Mandibule/chirurgie , Transplantation de cellules souches mésenchymateuses , Cellules souches mésenchymateuses/cytologie , Animaux , Trame osseuse , Cellules cultivées , Interprétation statistique de données , Mandibule/cytologie , Lapins , Facteurs temps , TitaneRÉSUMÉ
With the help of flow cytometry it was shown that for patients with hypergic inflammatory process in maxillofacial region the reduction of lymphocytes relative number was typical as well as their absolute number reduction. Direct and very strong correlation was established between absolute lymphocytes number in peripheral blood and absolute number of CD3 and CD4 lymphocytes subpopulations by correlation analyses. The reduction of absolute lymphocytes number in peripheral blood let speak with more probability about the reduction of the number of CD3 and CD4 lymphocytes subpopulations and diagnose secondary immune deficit.
Sujet(s)
Cellulite sous-cutanée/traitement médicamenteux , Cellulite sous-cutanée/immunologie , Dermatoses faciales/traitement médicamenteux , Dermatoses faciales/immunologie , Facteurs immunologiques/usage thérapeutique , Numération des lymphocytes CD4 , Cellulite sous-cutanée/sang , Dermatoses faciales/sang , Cytométrie en flux , Humains , Déficits immunitaires/étiologie , Mâchoire , Hyperleucocytose/étiologie , Numération des lymphocytes , Hyperlymphocytose/étiologie , Lymphopénie/étiologie , CouRÉSUMÉ
Aim of the study was substantiation in experiments on rabbit ramus mandibulae of the method of mesenchymal stem cells (MSC) on the carrier from titanium and gold alloy for reparative osteogenesis enforcement. There was shown stimulating action of the MSC implantation in the bone wound upon reparative osteogenesis at the terms from 1 to 4 months. It was concluded that implants from titanium and gold alloy may be used as carrier for MSC for bone tissue reparative regeneration stimulation.
Sujet(s)
Alliage dentaire/usage thérapeutique , Or/usage thérapeutique , Mandibule/chirurgie , Traumatismes mandibulaires/chirurgie , Transplantation de cellules souches mésenchymateuses , Titane/usage thérapeutique , Animaux , Génie biomédical , Régénération osseuse , Mandibule/anatomopathologie , Mandibule/physiologie , Traumatismes mandibulaires/anatomopathologie , Traumatismes mandibulaires/physiopathologie , RatsRÉSUMÉ
In experimental-morphological study on 6 dogs the dynamics of regenerate formation in ulna and mandible defects after implantation in them composite material of different density (0.46-0.50 and 0.38-0.42 g/cm(3)) of polylactide (PL) origin filled with HAP was followed at the terms of 6 and 9 months. Histologic study and structural determinant distribution analysis in the content of regenerate showed that optimal results according to the bone defect substitution by bone regenerate criterion at 9th month of the experiment were received after composite material from PL and HAP with the density of 0.38-0.42 g/cm(3) implantation. Newly formed trabecular bone tissue was seen in the regenerate and as well as strong tendency for bone matrix maturation. It was confirmed by the appearance of lamellar structures in newly formed bone trabecules situated in peripheral zones of bone defect. In bone mandible defects the substitution process of the implants from PL with HAP by the bone tissue was much slower than in ulna defects.
Sujet(s)
Implant résorbable , Régénération osseuse , Hydroxyapatites/usage thérapeutique , Mandibule/physiologie , Traumatismes mandibulaires/physiopathologie , Polyesters/usage thérapeutique , Animaux , Chiens , Mandibule/chirurgie , Mandibule/ultrastructure , Traumatismes mandibulaires/anatomopathologie , Traumatismes mandibulaires/chirurgieRÉSUMÉ
We compared the morphology and differentiation characteristics of the human cells from bone marrow, adipose tissue, hair papilla and skin dermis. All cell types showed fibroblastic morphology. Immunofluorescent analysis showed that adipose tissue derived stem cells (ADAS) and hair papilla cells (HPC) expressed CD105, CD49d and STRO-1, bone marrow mesenchymal stem cells (BMSC) were absent for CD49, dermal fibroblasts (DFb) expressed CD49 and STRO-1 at low level. Populations of ADAS, BMSC and HPC had similar capacities to lipid and bone differentiation. Following exposure to appropriate induction stimuli, these cells changed phenotype and expressed specific cell markers. However, the rate and extent of HPC differentiation were lower in comparison with populations of ADAS and BMSC. We propose that all investigated cell populations contain primitive progenitor cells with mesenchymal stem cell properties.
Sujet(s)
Tissu adipeux/cytologie , Cellules de la moelle osseuse/cytologie , Différenciation cellulaire , Derme/cytologie , Fibroblastes/cytologie , Follicule pileux/cytologie , Cellules stromales/cytologie , Tissu adipeux/immunologie , Antigènes CD/immunologie , Cellules de la moelle osseuse/immunologie , Techniques de culture cellulaire , Différenciation cellulaire/immunologie , Cellules cultivées , Derme/immunologie , Fibroblastes/immunologie , Follicule pileux/immunologie , Humains , Immunohistochimie , Cellules stromales/immunologieRÉSUMÉ
Histamine releasing and immunomodulating activity of the following dental restorative materials (DRM) - Prizmafil, Filtek Z250, XRV Herculite Prodigy, Glasiosite, Te-Econol, Valux Plus, Polofil Supra were studied. It was shown that DRM under study as a rule did not possess the ability to release histamine (H) from human blood basophile (BB) excluding Filtek Z250 which release H from BB in patients with allergy and sound donors. The studied DRM implanted under mouse skin were able to modulate immune response to the allergen, at that some of them increased antibodies of IgE-class forming and other suppressed immune response caused by IgG-antibody forming. Received data have certain significance in the scheme of safety evaluation and individual assessment of DRM having acceptable biocompatibility for specific patient.
Sujet(s)
Résines acryliques/pharmacologie , Lymphocytes B/effets des médicaments et des substances chimiques , Résines composites/pharmacologie , Histamine/métabolisme , Immunoglobulines/effets des médicaments et des substances chimiques , Alliages métal céramique/pharmacologie , Polyuréthanes/pharmacologie , Animaux , Histamine/biosynthèse , Humains , Souris , RatsRÉSUMÉ
In rat experiments the ability of noncollagen bone proteins (NCBP) in the composition of osteoplactic modified material Gapkol (not tanned in formalin and subjected to vacuum extraction) to increase bone reparation in comparison with traditional Gapkol was studied. Quantitative evaluation was performed on rat parietal bone and qualitative evaluation was performed on rat mandible. It was shown that Gapkol with NCBP (not tanned in formalin and subjected to vacuum extraction) increased reparative osteogenesis.
Sujet(s)
Substituts osseux/administration et posologie , Os et tissu osseux/composition chimique , Collagène/pharmacologie , Durapatite/pharmacologie , Protéines et peptides de signalisation intercellulaire/physiologie , Mandibule/effets des médicaments et des substances chimiques , Ostéogenèse/physiologie , Protéines/analyse , Vide , Animaux , Protéines morphogénétiques osseuses/métabolisme , Calcification physiologique/physiologie , Collagène/administration et posologie , Association médicamenteuse , Durapatite/administration et posologie , Inlays , Mâle , Mandibule/métabolisme , Os occipital/effets des médicaments et des substances chimiques , Os occipital/métabolisme , Rats , Rat WistarRÉSUMÉ
Different types of dental materials (DM) were studied for their capacity to release histamine in vitro from basophils of whole blood of allergic patients and healthy donors using automated and computerized glass fiber-based leukocyte histamine release test (LHRT). It was shown that some types of DM possessed ability to release histamine from basophils and some didn't. There were no differences in histamine releaseability from basophils obtained from allergic patients and healthy donors. LHRT gives opportunity to recognize of DM possessing high or low histamine releaseability as well as to detect individual sensitivity to different DM. Thus, LHRT can be used for preliminary assessment of DM for their biocompatibility and also for individual selection of suitable DM for particular patient to avoid unwanted side effects.
Sujet(s)
Résines acryliques/composition chimique , Granulocytes basophiles/métabolisme , Matériaux biocompatibles/composition chimique , Matériaux dentaires/composition chimique , Libération d'histamine/physiologie , Histamine/métabolisme , Test de matériaux/méthodes , Humains , Hypersensibilité/sang , Spectrométrie de fluorescenceRÉSUMÉ
Math modelling "removable dentures-implantate-bone" with size and density of bone tissue as variables was created. It allowed to study biomechanical bases of mandibular bone tissue structures interaction with full removable dentures of different constructions and fixed on intraosseous implantates. Analysis of the received data showed that in the majority of cases it was expedient to recommend 3 bearing (abutments) system of denture making. Rest on 4 and more implantates was appropriate for patients with reduced density of spongy bone and significant mandibular bone atrophy. 2 abutment system can be used in patients with high density of spongy bone and absence of mandibular bone atrophy.
Sujet(s)
Densité osseuse/physiologie , Pose d'implant dentaire endo-osseux/instrumentation , Prothèse dentaire implanto-portée/normes , Mandibule/imagerie diagnostique , Maladies mandibulaires/chirurgie , Test de matériaux/méthodes , Modèles théoriques , Phénomènes biomécaniques , Humains , Maladies mandibulaires/imagerie diagnostique , RadiographieRÉSUMÉ
Digital microfocal x-ray study was experimentally studied in animals to examine the time course of changes in their bone regeneration. Sixteen Chinchila rabbits whose bone defect in the angle of the mandibular ramus had been closed with the osteoplastic material Gapcol with the applied allogeneic, autologous stem cells isolated from rabbit adipose tissue and human plasma enriched with thrombocytic growth factors were examined. The capabilities of digital microfocal x-ray study versus x-ray computed tomography were compared in the evaluation of reparative regeneration of bone tissue. The results of radiation studies were verified with the data of scanning electron microscopy.
Sujet(s)
Régénération osseuse , Os et tissu osseux/imagerie diagnostique , Os et tissu osseux/physiologie , Amélioration d'image radiographique/méthodes , Animaux , Humains , Lapins , TomodensitométrieSujet(s)
Antigènes CD/physiologie , Cellulite sous-cutanée/immunologie , Cellulite sous-cutanée/anatomopathologie , Lymphocytes/physiologie , Maladies du maxillaire supérieur/immunologie , Maladies du maxillaire supérieur/anatomopathologie , Adolescent , Adulte , Sujet âgé , Antigènes CD/immunologie , Femelle , Humains , Lymphocytes/immunologie , Mâle , Adulte d'âge moyen , Jeune adulteRÉSUMÉ
Dysbiotic shifts in intestinal and integumentary microflora were studied in 10 test-subjectes during 7-day "dry" immersion. Essentially every test-subject reduced significantly fecal lactoflora and developed dysbiotic shifts in the microbial landscape of various types of integument. Pharyngeal microflora was analyzed in 22 normal volunteers for 7-day "dry" immersion. Two probiotic preparations were administered to prevent pharyngeal dysbiosis: peroral dry lactobacterin and topical collagen-immobilized lactobacterin. The peroral probiotic stimulated growth of pharyngeal opportunistic pathogens preventing translocations of the intestinal microflora. The topical probiotic, on the opposite, reduced the content of opportunistic pathogens in the throat; however, it provoked gastrointestinal dysbiosis. It appears that the most effective prophylaxis of pharyngeal dysbiosis can be reached by prescription of both topical and peroral probiotics.
Sujet(s)
Infections bactériennes/prévention et contrôle , Immersion/effets indésirables , Intestins/microbiologie , Probiotiques/usage thérapeutique , Adolescent , Adulte , Anti-infectieux/usage thérapeutique , Infections bactériennes/étiologie , Bactériocines/usage thérapeutique , Études de suivi , Humains , Lactobacillus/isolement et purification , Valeurs de référence , Résultat thérapeutique , Jeune adulteRÉSUMÉ
Influence of HAP-gel (2 g of 2% solution of hyaluronic acid mixed with 0,5 g of hydroxyapatite and 0,1 ml of colloidal silver) upon osseointegration in case of delayed introduction of titanium implantates in dog jaw. By scanning electron microscopy it was shown that solely use either of HAP-gel or of ceramic spraying increased direct contact area between bone and implantates in the 6 and 9 months time period. Combination of spray-coated ceramic with HAP-gel was effective in 3 months after implantation, when solely the HAP-gel or the ceramic spraying were little effective. In the following terms of experiment (6 and 9 months) significant differences between groups 3 and 4 (implantate with ceramic spraying but without HAP-gel in the alveolus and implantate with ceramic spraying and with HAP-gel in the alveolus) were not found. The area of implantate integration with jaw bone (cortical part of it was excluded) was equal to 80% and was maximal for the given conditions of the experiment.