The complete mitochondrial genome of Agriotes fuscicollis Miwa, 1928 (Coleoptera: Elateridae).

In this study, we sequenced the complete mitochondrial genome of Agriotes fuscicollis Miwa, 1928 (Coleoptera: Elateridae). The results showed that the length of complete mitochondrial genome was 15,866 bp with 26.8% GC content, containing 39.6% A, 33.5% T, 16.8% C, 10% G. There were 13 protein-coding genes (PCGs), 22 transfer RNA genes, and 2 ribosomal RNA genes. Phylogenetic analysis showed that A. fuscicollis was closely related to Cryptalaus larvatus, Cryptalaus yamato, Pyrophorus divergens and Ignelater luminosus. The complete mitogenome of A. fuscicollis would contribute to the study of the phylogeny and evolution of Elateridae.

A Case Study of Refined Building Climate Zoning under Complicated Terrain Conditions in China.

In this study, we first found that the few and sparse meteorological stations used in earlier comprehensive studies of building climate zoning in a complicated terrain area like Chongqing, China, may lead to the inapplicability of building energy efficiency standards in some areas. To address this issue, the study used daily data from 1908 extremely dense surface meteorological stations from 2011 to 2020 in Chongqing, China. In order to conduct fine zoning of building climate in Chongqing, China, GB50176-2016 and ASHRAE standard 169-2021 were employed, respectively. The findings indicated that by using the ASHRAE standard, the entire Chongqing region was classified into five climate zones. The Chongqing region was categorized into three different climate zones using China GB50176-2016: cold zone (CZ), hot summer and cold winter zone (HSCWZ), and mild zone (MZ). Not to be overlooked is the MZ (China's GB50176-2016)/mixed-humid zone (ASHRAE standard), which is primarily situated at higher elevations in the southeast and northeast of Chongqing. In comparison to the HSCWZ/warm-humid zone, these zones have drastically different building energy efficiency regulations and approaches. According to preliminary projections, improved building climate zoning will to some extent increase building energy efficiency and reduce emissions in Chongqing. Finally, this study case can be replicated in different regions with complicated terrain.

Drought induces epitranscriptome and proteome changes in stem-differentiating xylem of Populus trichocarpa.

Understanding gene expression and regulation requires insights into RNA transcription, processing, modification, and translation. However, the relationship between the epitranscriptome and the proteome under drought stress remains undetermined in poplar (Populus trichocarpa). In this study, we used Nanopore direct RNA sequencing and tandem mass tag-based proteomic analysis to examine epitranscriptomic and proteomic regulation induced by drought treatment in stem-differentiating xylem (SDX). Our results revealed a decreased full-length read ratio under drought treatment and, especially, a decreased association between transcriptome and proteome changes in response to drought. Epitranscriptome analysis of cellulose- and lignin-related genes revealed an increased N6-Methyladenosine (m6A) ratio, which was accompanied by decreased RNA abundance and translation, under drought stress. Interestingly, usage of the distal poly(A) site increased during drought stress. Finally, we found that transcripts of highly expressed genes tend to have shorter poly(A) tail length (PAL), and drought stress increased the percentage of transcripts with long PAL. These findings provide insights into the interplay among m6A, polyadenylation, PAL, and translation under drought stress in P. trichocarpa SDX.

Simulation and Projection of Climate Extremes in China by a Set of Statistical Downscaled Data.

This study assesses present-day extreme climate changes over China by using a set of phase 6 of the Coupled Model Intercomparison Project (CMIP6) statistical downscaled data and raw models outputs. The downscaled data is produced by the adapted spatial disaggregation and equal distance cumulative distribution function (EDCDF) method at the resolution of 0.25° × 0.25° for the present day (1961-2014) and the future period (2015-2100) under the Shared Socioeconomic Path-way (SSP) 2-4.5 than SSP5-8.5 emission scenario. The results show that the downscaling method improves the spatial distributions of extreme climate events in China with higher spatial pattern correlations, Taylor Skill Scores and closer magnitudes no matter single model or multi model ensemble (MME). In the future projections, large inter-model variability between the downscaled models still exists, particular for maximum consecutive 5-day precipitation (RX5). The downscaled MME projects that total precipitation (PTOT) and RX5, will increase with time, especially for the northwest China. The projected heavy precipitation days (R20) also increase in the future. The region of significant increase in R20 locates in the south of river Yangtze. Maxi-mum annual temperature (TXX) and percentage of warm days (TX90p) are projected to increase across the whole country with larger magnitude over the west China. Projected changes of minimum annual temperature (TNN) over the northeastern China is the most significant area. The higher of the emission scenario, the more significant of extreme climates. This reveals that the spatial distribution of extreme climate events will become more uneven in the future.