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1.
Am J Hum Genet ; 2024 Aug 28.
Article de Anglais | MEDLINE | ID: mdl-39226898

RÉSUMÉ

Variants that alter gene splicing are estimated to comprise up to a third of all disease-causing variants, yet they are hard to predict from DNA sequencing data alone. To overcome this, many groups are incorporating RNA-based analyses, which are resource intensive, particularly for diagnostic laboratories. There are thousands of functionally validated variants that induce mis-splicing; however, this information is not consolidated, and they are under-represented in ClinVar, which presents a barrier to variant interpretation and can result in duplication of validation efforts. To address this issue, we developed SpliceVarDB, an online database consolidating over 50,000 variants assayed for their effects on splicing in over 8,000 human genes. We evaluated over 500 published data sources and established a spliceogenicity scale to standardize, harmonize, and consolidate variant validation data generated by a range of experimental protocols. According to the strength of their supporting evidence, variants were classified as "splice-altering" (∼25%), "not splice-altering" (∼25%), and "low-frequency splice-altering" (∼50%), which correspond to weak or indeterminate evidence of spliceogenicity. Importantly, 55% of the splice-altering variants in SpliceVarDB are outside the canonical splice sites (5.6% are deep intronic). These variants can support the variant curation diagnostic pathway and can be used to provide the high-quality data necessary to develop more accurate in silico splicing predictors. The variants are accessible through an online platform, SpliceVarDB, with additional features for visualization, variant information, in silico predictions, and validation metrics. SpliceVarDB is a very large collection of splice-altering variants and is available at https://splicevardb.org.

2.
Article de Anglais | MEDLINE | ID: mdl-39225840

RÉSUMÉ

PURPOSE: Identification of mature sperm at microdissection testicular sperm extraction (mTESE) is a crucial step of sperm retrieval to help patients with non-obstructive azoospermia (NOA) proceed to intracytoplasmic sperm injection. Touch print smear (TPS) cytology allows immediate interpretation and prompt sperm identification intraoperatively. In this study, we leverage machine learning (ML) to facilitate TPS reading and conquer the learning curve for new operators. MATERIALS AND METHODS: One hundred seventy-six microscopic TPS images from the testicular specimen of patients with azoospermia at Taipei Veterans General Hospital were retrospectively collected, including categories of Sertoli cell, primary spermatocytes, round spermatids, elongated spermatids, immature sperm, and mature sperm. Among them, 118 images were assigned as the training set and 29 images as the validation set. RetinaNet (Lin et al. in IEEE Trans Pattern Anal Mach Intell. 42:318-327, 2020), a one-stage detection framework, was adopted for cell detection. The performance was evaluated at the cell level with average precision (AP) and recall, and the precision-recall (PR) curve was displayed among an independent testing set that contains 29 images that aim to assess the model. RESULTS: The training set consisted of 4772 annotated cells, including 1782 Sertoli cells, 314 primary spermatocytes, 443 round spermatids, 279 elongated spermatids, 504 immature sperm, and 1450 mature sperm. This study demonstrated the performance of each category and the overall AP and recall on the validation set, which were 80.47% and 96.69%. The overall AP and recall were 79.48% and 93.63% on the testing set, while increased to 85.29% and 93.80% once the post-meiotic cells were merged into one category. CONCLUSIONS: This study proposed an innovative approach that leveraged ML methods to facilitate the diagnosis of spermatogenesis at mTESE for patients with NOA. With the assistance of ML techniques, surgeons could determine the stages of spermatogenesis and provide timely histopathological diagnosis for infertile males.

3.
Int J Mol Sci ; 25(17)2024 Sep 07.
Article de Anglais | MEDLINE | ID: mdl-39273633

RÉSUMÉ

The maize Snf2 gene family plays a crucial role in chromatin remodeling and response to environmental stresses. In this study, we identified and analyzed 35 members of the maize Snf2 gene family (ZmCHR1 to ZmCHR35) using the Ensembl Plants database. Each protein contained conserved SNF2-N and Helicase-C domains. Phylogenetic analysis revealed six groups among the Snf2 proteins, with an uneven distribution across subfamilies. Physicochemical analysis indicated that the Snf2 proteins are hydrophilic, with varied amino acid lengths, isoelectric points, and molecular weights, and are predominantly localized in the nucleus. Chromosomal mapping showed that these genes are distributed across all ten maize chromosomes. Gene structure analysis revealed diverse exon-intron arrangements, while motif analysis identified 20 conserved motifs. Collinearity analysis highlighted gene duplication events, suggesting purifying selection. Cis-regulatory element analysis suggested involvement in abiotic and biotic stress responses. Expression analysis indicated tissue-specific expression patterns and differential expression under various stress conditions. Specifically, qRT-PCR validation under drought stress showed that certain Snf2 genes were upregulated at 12 h and downregulated at 24 h, revealing potential roles in drought tolerance. These findings provide a foundation for further exploration of the functional roles of the maize Snf2 gene family in development and stress responses.


Sujet(s)
Régulation de l'expression des gènes végétaux , Famille multigénique , Phylogenèse , Protéines végétales , Stress physiologique , Zea mays , Zea mays/génétique , Zea mays/métabolisme , Stress physiologique/génétique , Protéines végétales/génétique , Protéines végétales/métabolisme , Sécheresses , Chromosomes de plante/génétique , Cartographie chromosomique , Facteurs de transcription/génétique , Facteurs de transcription/métabolisme
4.
ISA Trans ; 2024 Sep 12.
Article de Anglais | MEDLINE | ID: mdl-39278813

RÉSUMÉ

To reduce the cross-regulation and improve the dynamic response performance of a single-inductor double-output Boost converter, an adaptive backstepping sliding mode control (ABSMC) strategy is proposed in this paper. The nonlinear mathematical model of the converter is established, an output function satisfying the exact feedback linearization (EFL) is constructed based on the inverse system theory, and the linearization and decoupling of the model are implemented. Meanwhile, the problem of EFL heavily relying on an exact model is solved by combining backstepping control with sliding mode control. Furthermore, an adaptive reaching law is proposed to adjust the gain of the switching function, and the chattering phenomenon of sliding mode control is reduced. The stability of the system is proven according to the Lyapunov stability theorem. Finally, compared with the existing control methods, both the simulation results and experimental results verify the effectiveness and superiority of the proposed ABSMC strategy.

5.
PLoS One ; 19(6): e0304691, 2024.
Article de Anglais | MEDLINE | ID: mdl-38833435

RÉSUMÉ

With the rapid development of intelligent connected vehicles, there is an increasing demand for hardware facilities and onboard systems of driver assistance systems. Currently, most vehicles are constrained by the hardware resources of onboard systems, which mainly process single-task and single-sensor data. This poses a significant challenge in achieving complex panoramic driving perception technology. While the panoramic driving perception algorithm YOLOP has achieved outstanding performance in multi-task processing, it suffers from poor adaptability of feature map pooling operations and loss of details during downsampling. To address these issues, this paper proposes a panoramic driving perception fusion algorithm based on multi-task learning. The model training involves the introduction of different loss functions and a series of processing steps for lidar point cloud data. Subsequently, the perception information from lidar and vision sensors is fused to achieve synchronized processing of multi-task and multi-sensor data, thereby effectively improving the performance and reliability of the panoramic driving perception system. To evaluate the performance of the proposed algorithm in multi-task processing, the BDD100K dataset is used. The results demonstrate that, compared to the YOLOP model, the multi-task learning network performs better in lane detection, drivable area detection, and vehicle detection tasks. Specifically, the lane detection accuracy improves by 11.6%, the mean Intersection over Union (mIoU) for drivable area detection increases by 2.1%, and the mean Average Precision at 50% IoU (mAP50) for vehicle detection improves by 3.7%.


Sujet(s)
Algorithmes , Conduite automobile , Humains , Conduite automobile/psychologie , Analyse et exécution des tâches
6.
Front Psychol ; 15: 1371936, 2024.
Article de Anglais | MEDLINE | ID: mdl-38840737

RÉSUMÉ

Introduction: Career construction theory proposes that adaptivity affects career adapting through career adaptability. However, research on the mechanism of this pathway remains scarce. By applying career construction theory and conservation of resources theory, we hypothesize that career adaptability (concern, control, curiosity, and confidence) mediates the relationship between empathetic leadership (adaptivity) and innovative behavior (career adapting). Moreover, we posit that uncertain avoidance moderates the mediating mechanism. Methods: Our study used SPSS23 and bootstrap methods (PROCESS) to test the proposed model. The sample comprised 301 employees from different firms in various industries. In this study, empathetic leadership-5, career adaptability-24, uncertainty avoidance-5, and innovative behavior-6 scales were used to measure empathetic leadership, career adaptability, and uncertainty avoidance. Results: The results revealed that (1) empathetic leadership is positively related to employees' innovative behavior (2) Concern (H2a), control (H2b), curiosity (H2c), and confidence (H2d) mediate the relations between empathetic leadership and employee's innovative behavior (3) Uncertainty avoidance moderates the relationship between empathetic leadership and concern (H3a), control (H3b), curiosity (H3c), and confidence (H3d), such that this relationship is stronger when uncertainty avoidance is higher (4) Uncertainty avoidance moderates the indirect relationship between empathetic leadership and employee innovative behavior through concern (H4a), control (H4b), curiosity (H4c), and confidence (H4d), such that this indirect relationship will be stronger when uncertainty avoidance is high than when it is low. Conclusion: We investigated how empathetic leadership affects innovative behavior. Based on career construction theory and conservation of resources theory, we also tested the hypothesis that concern, control, curiosity, and confidence play mediating roles in linking empathetic leadership (career adaptivity) to innovative behavior (career adapting). In addition, this study found that uncertainty avoidance does not always have negative effects. People with a high uncertainty avoidance tendency may be dependent more on empathetic leadership to improve their career adaptability, which promotes their innovative behavior.

7.
Adv Sci (Weinh) ; 11(31): e2402050, 2024 Aug.
Article de Anglais | MEDLINE | ID: mdl-38889249

RÉSUMÉ

The extraction of targets from biological samples for immunoassays using organic solvents, such as methanol, is often necessary. However, high concentrations of organic solvents in extracts invariably lead to instability of the employed antibody, resulting in poor performance of the immunoassay. Evaluating the tolerance ability and exploring the molecular mechanisms of antibody tolerance in organic solvents are essential for the development of robust immunoassays. In this work, 25 monoclonal antibodies and methanol are utilized as models to address these questions. A novel protocol is initially established to precisely and rapidly determine antibody tolerance in methanol, identifying two distinct methanol effect patterns. Through a detailed investigation of the structural basis, a novel hypothesis regarding methanol effect patterns is proposed, termed "folding-aggregation," which is subsequently validated through molecular dynamics simulations. Furthermore, the investigation of sequence basis reveals significant differences in residue types within the complementarity-determining regions and ligand-binding residues, distinguishing the two antibody methanol effect patterns. Moreover, the methanol effect patterns of the antibodies are defined by germline antibodies. This work represents the first exploration of antibody methanol effect patterns and associated molecular mechanisms, with potential implications for the discovery and engineering of tolerant antibodies for the development of robust immunoassays.


Sujet(s)
Anticorps monoclonaux , Méthanol , Simulation de dynamique moléculaire , Solvants , Méthanol/composition chimique , Anticorps monoclonaux/immunologie , Solvants/composition chimique , Dosage immunologique/méthodes
8.
J Am Chem Soc ; 146(21): 14422-14426, 2024 May 29.
Article de Anglais | MEDLINE | ID: mdl-38709624

RÉSUMÉ

Here we report a concise and divergent synthesis of scabrolide A and havellockate, representative members of polycyclic marine natural product furano(nor)cembranoids. The synthesis features a highly efficient exo-exo-endo radical cascade. Through the generation of two rings, three C-C bonds, and three contiguous stereocenters in one step, this remarkable transformation not only assembles the bowl-shaped, common 6-5-5 fused ring system from simple building blocks but also precisely installs the functionalities at desired positions and sets the stage for further divergent preparation of both target molecules. Further studies reveal that the robust and unusual 6-endo radical addition in the cascade is likely facilitated by the rigidity of the substrate.

9.
J Glob Antimicrob Resist ; 38: 1-5, 2024 Sep.
Article de Anglais | MEDLINE | ID: mdl-38777179

RÉSUMÉ

BACKGROUND: Enterococcus faecium (E. faecium) stands as a prominent pathogen contributing to Gram-positive bacterial infections in individuals who have undergone liver transplantation. CASE PRESENTATION: A 66-year-old male with a three-year history of treated anxiety disorder was admitted to our hospital due to recurrent abdominal distension and oliguria. He was diagnosed with hepatic veno-occlusive disease (HVOD), hepatic failure, pneumonia, renal insufficiency and abdominal ascites. A liver transplantation procedure was performed, but the patient's infection index increased on the first day after surgery. Empirical antibiotic therapy with ceftriaxone and meropenem and preventive antifungal therapy were applied. Sputum culture, blood culture, ascites culture and bronchoalveolar lavage fluid (BALF) next-generation sequencing (NGS), revealed the presence of E. faecium. Given the application of various nephrotoxic immunosuppressive agents after liver transplantation, pre-existing renal insufficiency, severe bone marrow suppression, and a history of anxiety disorder treated with sertraline, contezolid was added for the treatment of the Gram-positive bacterial infection. Sixteen days after surgery, cultures from ascites and sputum yielded negative results for fungi and bacteria. Contezolid was subsequently discontinued without any reported adverse events during follow-up. CONCLUSION: Treatment with contezolid as the first-line therapy for sepsis and pneumonia caused by E. faecium following liver transplantation has shown satisfactory efficacy and safety. Therefore, contezolid may hold great promise for managing this life-threatening condition.


Sujet(s)
Antibactériens , Enterococcus faecium , Infections bactériennes à Gram positif , Transplantation hépatique , Sepsie , Humains , Mâle , Enterococcus faecium/effets des médicaments et des substances chimiques , Enterococcus faecium/isolement et purification , Transplantation hépatique/effets indésirables , Sujet âgé , Infections bactériennes à Gram positif/microbiologie , Infections bactériennes à Gram positif/traitement médicamenteux , Sepsie/microbiologie , Sepsie/étiologie , Sepsie/traitement médicamenteux , Antibactériens/usage thérapeutique , Pneumopathie infectieuse/microbiologie , Pneumopathie infectieuse/traitement médicamenteux , Pneumopathie infectieuse/étiologie
10.
BMC Plant Biol ; 24(1): 390, 2024 May 11.
Article de Anglais | MEDLINE | ID: mdl-38730367

RÉSUMÉ

Granulation of juice sacs is a physiological disorder, which affects pomelo fruit quality. Here, the transcriptome and ubiquitinome of the granulated juice sacs were analyzed in Guanxi pomelo. We found that lignin accumulation in the granulated juice sacs was regulated at transcription and protein modification levels. In transcriptome data, we found that the genes in lignin biosynthesis pathway and antioxidant enzyme system of the granulated juice sacs were significantly upregulated. However, in ubiquitinome data, we found that ubiquitinated antioxidant enzymes increased in abundance but the enzyme activities decreased after the modification, which gave rise to reactive oxygen species (ROS) contents in granulated juice sacs. This finding suggests that ubiquitination level of the antioxidant enzymes is negatively correlated with the enzyme activities. Increased H2O2 is considered to be a signaling molecule to activate the key gene expressions in lignin biosynthesis pathway, which leads to the lignification in granulated juice sacs of pomelo. This regulatory mechanism in juice sac granulation of pomelo was further confirmed through the verification experiment using tissue culture by adding H2O2 or dimethylthiourea (DMTU). Our findings suggest that scavenging H2O2 and other ROS are important for reducing lignin accumulation, alleviating juice sac granulation and improving pomelo fruit quality.


Sujet(s)
Citrus , Lignine , Lignine/métabolisme , Citrus/métabolisme , Citrus/génétique , Jus de fruits et de légumes/analyse , Espèces réactives de l'oxygène/métabolisme , Transcriptome , Peroxyde d'hydrogène/métabolisme , Régulation de l'expression des gènes végétaux , Fruit/métabolisme , Fruit/génétique , Antioxydants/métabolisme
11.
Sci China Life Sci ; 67(7): 1413-1426, 2024 Jul.
Article de Anglais | MEDLINE | ID: mdl-38565741

RÉSUMÉ

Endocrine therapy that blocks estrogen signaling is the most effective treatment for patients with estrogen receptor positive (ER+) breast cancer. However, the efficacy of agents such as tamoxifen (Tam) is often compromised by the development of resistance. Here we report that cytokines-activated nuclear IKKα confers Tam resistance to ER+ breast cancer by inducing the expression of FAT10, and that the expression of FAT10 and nuclear IKKα in primary ER+ human breast cancer was correlated with lymphotoxin ß (LTB) expression and significantly associated with relapse and metastasis in patients treated with adjuvant mono-Tam. IKKα activation or enforced FAT10 expression promotes Tam-resistance while loss of IKKα or FAT10 augments Tam sensitivity. The induction of FAT10 by IKKα is mediated by the transcription factor Pax5, and coordinated via an IKKα-p53-miR-23a circuit in which activation of IKKα attenuates p53-directed repression of FAT10. Thus, our findings establish IKKα-to-FAT10 pathway as a new therapeutic target for the treatment of Tam-resistant ER+ breast cancer.


Sujet(s)
Tumeurs du sein , Résistance aux médicaments antinéoplasiques , I-kappa B Kinase , Transduction du signal , Tamoxifène , Animaux , Femelle , Humains , Antinéoplasiques hormonaux/pharmacologie , Antinéoplasiques hormonaux/usage thérapeutique , Tumeurs du sein/métabolisme , Tumeurs du sein/traitement médicamenteux , Tumeurs du sein/génétique , Lignée cellulaire tumorale , Cytokines/métabolisme , Résistance aux médicaments antinéoplasiques/génétique , Régulation de l'expression des gènes tumoraux/effets des médicaments et des substances chimiques , I-kappa B Kinase/métabolisme , Cellules MCF-7 , Transduction du signal/effets des médicaments et des substances chimiques , Tamoxifène/pharmacologie , Tamoxifène/usage thérapeutique , Protéine p53 suppresseur de tumeur/métabolisme , Protéine p53 suppresseur de tumeur/génétique
12.
J Hazard Mater ; 469: 133977, 2024 May 05.
Article de Anglais | MEDLINE | ID: mdl-38492395

RÉSUMÉ

The immunogenicity of haptens determines the performance of the resultant antibody for small molecules. Rigidity is one of the basic physicochemical properties of haptens. However, few studies have investigated the effect of hapten rigidity on the strength of an immune response and overall antibody performance. Herein, we introduce three molecular descriptors that quantify hapten rigidity. By using of these descriptors, four rifamycin haptens with varied rigidity were designed. The structural and physicochemical feasibility of the designed haptens was then assessed by computational chemistry. Immunization demonstrated that the strength of induced immune responses, i.e., the titer and affinity of antiserum, was significantly increased with increased rigidity of haptens. Furthermore, molecular dynamic simulations demonstrated conformation constraint of rigid haptens contributed to the initial binding and activation of naïve B cells. Finally, a highly sensitive indirect competitive enzyme-linked immunosorbent assay was developed for detection of rifaximin, with an IC50 of 1.1 µg/L in buffer and a limit of detection of 0.2-11.3 µg/L in raw milk, river water, and soil samples. This work provides new insights into the effect of hapten rigidity on immunogenicity and offers new hapten design strategies for antibody discovery and vaccine development of small molecules.


Sujet(s)
Anticorps , Rifamycine , Test ELISA , Dosage immunologique , Haptènes
13.
PLoS One ; 19(2): e0296782, 2024.
Article de Anglais | MEDLINE | ID: mdl-38306357

RÉSUMÉ

Based Correctly handling the creativity of employees who have not been adopted is not only conducive to continuously stimulating employees' creativity and improving individual innovation performance, but also conducive to making the best use of organizational resources. This study integrates conservation of resource theory (COR) and social information processing theory to explore the influence of bootleg innovation behavior in organizations on individual innovation performance, as well as the mediating role of cognitive flexibility and the moderating role of leadership emotional intelligence. A three-stage time-lagged research design is used to obtain a valid sample of 327 employees from China. The PROCESS macro for SPSS was applied to test the hypothesized relationships. Findings demonstrated that bootleg innovation is positively related to individual innovation performance; cognitive flexibility mediates the relationship between bootleg innovation and individual innovation performance. Moreover, leadership emotional intelligence moderates the relationship between bootleg innovation and individual innovation performance and between bootleg innovation and cognitive flexibility and between cognitive flexibility and individual innovation performance respectively. The conclusion of the study not only provides a theoretical basis for individuals and leaders to deal with employees' creative abortion, but also provides a new thinking mode for how to maximize the effectiveness of unaccepted ideas and promote individual innovation performance.


Sujet(s)
Avortement provoqué , Leadership , Femelle , Grossesse , Humains , Cognition , Chine , Intelligence émotionnelle
14.
PLoS One ; 19(2): e0293454, 2024.
Article de Anglais | MEDLINE | ID: mdl-38349897

RÉSUMÉ

In a dynamic and competitive business environment, managerial ability emerges as a pivotal strategic factor for capitalizing on new opportunities within the technological revolution and digital transformation of enterprises. Based on data from Chinese A-share listed firms spanning from 2009 to 2019, this study integrates insights from the upper echelons theory and the behavioral theory of the firm to investigate the moderating roles of historical aspiration shortfalls and industrial competitiveness on the relationship between managerial ability and enterprise digital transformation from internal and external pressure perspectives. Our findings indicate a positive impact of managerial ability on digital transformation. The relationship between managerial ability and digital transformation is reinforced by historical aspiration shortfalls; nevertheless, industrial competitiveness has attenuated the aforementioned relationship. This study contributes to a better understanding of the strategic implications of managerial ability within the context of organizational innovation strategies. It offers valuable insights into the decision-making processes of firms as they navigate the challenges of digital transformation within an ever-evolving business environment.

15.
PLoS One ; 19(1): e0298247, 2024.
Article de Anglais | MEDLINE | ID: mdl-38295085

RÉSUMÉ

[This corrects the article DOI: 10.1371/journal.pone.0295565.].

16.
PLoS One ; 18(12): e0295565, 2023.
Article de Anglais | MEDLINE | ID: mdl-38079443

RÉSUMÉ

Identification of sugarcane stem nodes is generally dependent on high-performance recognition equipment in sugarcane seed pre-cutting machines and inefficient. Accordingly, this study proposes a novel lightweight architecture for the detection of sugarcane stem nodes based on the YOLOv5 framework, named G-YOLOv5s-SS. Firstly, the study removes the CBS and C3 structures at the end of the backbone network to fully utilize shallow-level feature information. This enhances the detection performance of sugarcane stem nodes. Simultaneously, it eliminates the 32 times down-sampled branches in the neck structure and the 20x20 detection heads at the prediction end, reducing model complexity. Secondly, a Ghost lightweight module is introduced to replace the conventional convolution module in the BottleNeck structure, further reducing the model's complexity. Finally, the study incorporates the SimAM attention mechanism to enhance the extraction of sugarcane stem node features without introducing additional parameters. This improvement aims to enhance recognition accuracy, compensating for any loss in precision due to lightweight modifications. The experimental results showed that the average precision of the improved network for sugarcane stem node identification reached 97.6%, which was 0.6% higher than that of the YOLOv5 baseline network. Meanwhile, a model size of 2.6MB, 1,129,340 parameters, and 7.2G FLOPs, representing respective reductions of 82%, 84%, and 54.4%. Compared with mainstream one-stage target detection algorithms such as YOLOv4-tiny, YOLOv4, YOLOv5n, YOLOv6n, YOLOv6s, YOLOv7-tiny, and YOLOv7, G-YOLOv5s-SS achieved respective average precision improvements of 12.9%, 5.07%, 3.6%, 2.1%, 1.2%, 3%, and 0.4% in sugarcane stem nodes recognition. Meanwhile, the model size was compressed by 88.9%, 98.9%, 33.3%, 72%, 92.9%, 78.8% and 96.3%, respectively. Compared with similar studies, G-YOLOv5s-SS not only enhanced recognition accuracy but also considered model size, demonstrating an overall excellent performance that aligns with the requirements of sugarcane seed pre-cutting machines.


Sujet(s)
Saccharum , Algorithmes , Membrane érythrocytaire , , Cou
17.
Front Psychiatry ; 14: 1287217, 2023.
Article de Anglais | MEDLINE | ID: mdl-38076705

RÉSUMÉ

Introduction: As a common phenomenon of workplace negative gossip in organizations, how it affects employees' work engagement is not yet clear, nor what methods can be used to mitigate its negative impact on employees' work engagement. Methods: Based on Conservation of Resource Theory, this study obtained 334 valid employee samples from mainland China enterprises through a three-time lagged research design and explored the mechanism of negative workplace gossip on work engagement from the dual perspectives of employees and supervisors. Results: The results show that: (1) Negative workplace gossip negatively affects employee work engagement. (2) Professional commitment plays a mediating role between negative workplace gossip and employee work engagement. (3) Employee mindfulness negatively moderates the negative impact of workplace negative gossip on professional commitment; superior trust negatively moderates the negative impact of workplace negative gossip on professional commitment. (4) Employee mindfulness and superior trust are further weakened to moderate the negative indirect impact of workplace negative gossip on employee work engagement through professional commitment, and this negative indirect impact is weaker when employees have a higher degree of mindfulness and higher trust in superiors. Discussion: It proposes effective strategies for managing workplace gossip to harness its positive influence and offer practical guidance to enhance employee work engagement.

18.
PeerJ ; 11: e16254, 2023.
Article de Anglais | MEDLINE | ID: mdl-37920843

RÉSUMÉ

Armadillo (ARM) was a gene family important to plants, with crucial roles in regulating plant growth, development, and stress responses. However, the properties and functions of ARM family members in maize had received limited attention. Therefore, this study employed bioinformatics methods to analyze the structure and evolution of ARM-repeat protein family members in maize. The maize (Zea mays L.) genome contains 56 ARM genes distributed over 10 chromosomes, and collinearity analysis indicated 12 pairs of linkage between them. Analysis of the physicochemical properties of ARM proteins showed that most of these proteins were acidic and hydrophilic. According to the number and evolutionary analysis of the ARM genes, the ARM genes in maize can be divided into eight subgroups, and the gene structure and conserved motifs showed similar compositions in each group. The findings shed light on the significant roles of 56 ZmARM domain genes in development and abiotic stress, particularly drought stress. RNA-Seq and qRT-PCR analysis revealed that drought stress exerts an influence on specific members of the ZmARM family, such as ZmARM4, ZmARM12, ZmARM34 and ZmARM36. The comprehensive profiling of these genes in the whole genome, combined with expression analysis, establishes a foundation for further exploration of plant gene function in the context of abiotic stress and reproductive development.


Sujet(s)
Analyse de profil d'expression de gènes , Zea mays , Zea mays/génétique , Régions promotrices (génétique) , Protéines végétales/génétique , Stress physiologique/génétique
19.
Anal Chem ; 95(39): 14665-14674, 2023 10 03.
Article de Anglais | MEDLINE | ID: mdl-37679861

RÉSUMÉ

A sandwich immunoassay theoretically exhibits higher sensitivity and specificity compared to a competitive counterpart; however, it is extremely difficult to obtain a pair of antibodies that can bind to a small molecule simultaneously, which is always thought to be a single epitope. In the present study, abamectin (ABM) was selected to prove the effect of hapten design and antibody recognition properties on the development of a sandwich immunoassay for small molecules. First, the epitopes of ABM were roughly located, and epitope distances were determined. Then, two haptens were designed by introducing spacer arms at the C4″-OH and C5-OH of ABM, respectively, aiming to provide the longest epitope distances. A total of seven rabbit polyclonal antibodies (pAbs) and 21 mouse monoclonal antibodies (mAbs) with various recognition properties were obtained. Extensive combinatorial associations of antibody pairs for simultaneously binding to ABM were performed, and only two mAb-mAb pairs were observed to achieve a sandwich immunoassay for ABM with a total success rate of 0.27%. The best mAb pair for sandwich immunoassay was confirmed by surface plasmon resonance, used to develop a sandwich immunoassay, and then evaluated by cross-reactivities and molecular docking with structurally similar analogues and abamectin. Altogether, the study provided a theoretical foundation as well as practical experience and demonstrated the importance of careful hapten design and extensive antibody screening to successfully establish the sandwich immunoassay for small molecules.


Sujet(s)
Anticorps monoclonaux , Haptènes , Animaux , Souris , Lapins , Simulation de docking moléculaire , Anticorps monoclonaux/composition chimique , Dosage immunologique , Épitopes , Test ELISA
20.
Chemosphere ; 337: 139330, 2023 Oct.
Article de Anglais | MEDLINE | ID: mdl-37364645

RÉSUMÉ

Dissolved black carbon (DBC), an important photosensitizer in surface waters, can influence the photodegradation of various organic micropollutants. In natural water systems, DBC often co-occurs with metal ions as DBC-metal ion complexes; however, the influence of metal ion complexation on the photochemical activity of DBC is still unclear. Herein, the effects of metal ion complexation were investigated using common metal ions (Mn2+, Cr3+, Cu2+, Fe3+, Zn2+, Al3+, Ca2+, and Mg2+). Complexation constants (logKM) derived from three-dimensional fluorescence spectra revealed that Mn2+, Cr3+, Cu2+, Fe3+, Zn2+, and Al3+ quenched the fluorescence components of DBC via static quenching. The steady-state radical experiment suggested that in the complex systems of DBC with various metal ions, Mn2+, Cr3+, Cu2+, Fe3+, Zn2+ and Al3+ inhibited the photogeneration of 3DBC* via dynamic quenching, which reduced the yields of 3DBC*-derived 1O2 and O2·-. Moreover, 3DBC* quenching by metal ions was associated with the complexation constant. A strong positive linear relationship existed between logKM and the dynamic quenching rate constant of metal ions. These results indicate that the strong complexation ability of metal ions enabled 3DBC quenching, which highlights the photochemical activity of DBC in natural aquatic environments enriched with metal ions.


Sujet(s)
Métaux , Eau , Métaux/composition chimique , Ions , Photolyse , Carbone
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