Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 1 de 1
Filtrer
Plus de filtres











Gamme d'année
1.
Article de Chinois | WPRIM (Pacifique Occidental) | ID: wpr-355193

RÉSUMÉ

<p><b>OBJECTIVE</b>To obtain peptides binding specifically to Pisum sativum agglutinin (PSA) from a phage-displayed random peptide library.</p><p><b>METHODS</b>(1) A phage-displayed random hexapeptide library was screened with PSA as target. (2) Dot blot was used to analyze the influence of the alpha-Met-D-mannoside on binding between PSA and phage-displayed peptides. (3) Three peptides (RMWSF, RYDYSY, LRLRQL) were selectively synthesized, and different concentrations were used to inhibit PSA and ConA binding to the HRP.</p><p><b>RESULTS</b>The enrichment occurred obviously after three rounds of screening. The insert sequences of amino acids, displayed on 22 phage DNAs from the third round of screening, were divided into three groups. The binding of phage-displayed peptides to PSA was specific as shown by dot blot and could be inhibited by alpha-Met-D-mannoside. LRLRQL was not dissolved in water. ARMWSF and RYDYSY inhibited binding of PSA to HRP, but failed to inhibit binding ConA to HRP.</p><p><b>CONCLUSION</b>The binding site of peptides ARMWSF and RYDYSY is different to that of alpha-Met-D-mannoside.</p>


Sujet(s)
Sites de fixation , Banque de peptides , Peptides , Métabolisme , Lectines végétales , Métabolisme , Liaison aux protéines , Protéines recombinantes , Métabolisme
SÉLECTION CITATIONS
DÉTAIL DE RECHERCHE