RÉSUMÉ
The authors would like to replace Figure 4A of the following published paper [...].
RÉSUMÉ
Diabetic foot wound healing is a major clinical problem due to impaired angiogenesis and bacterial infection. Therefore, an effective regenerative dressing is desiderated with the function of promoting revascularization and anti-bacteria. Herein, a multifunctional injectable composite hydrogel was prepared by incorporation of the cerium-containing bioactive glass (Ce-BG) into Gelatin methacryloyl (GelMA) hydrogel. The Ce-BG was synthesized by combining sol-gel method with template method, which maintained spherical shape, chemical structure and phase constitution of bioactive glass (BG). The Ce-BG/GelMA hydrogels had good cytocompatibility, promoted endothelial cells migration and tube formation by releasing Si ion. In vitro antibacterial tests showed that 5 mol % CeO2-containing bioactive glass/GelMA (5/G) composite hydrogel exhibited excellent antibacterial properties. In vivo study demonstrated that the 5/G hydrogel could significantly improve wound healing in diabetic rats by accelerating the formation of granulation tissue, collagen deposition and angiogenesis. All in all, these results indicate that the 5/G hydrogel could enhance diabetic wound healing. Therefore, the development of multifunctional materials with antibacterial and angiogenic functions is of great significance to promote the repair of diabetic wound healing.
Sujet(s)
Antibactériens/administration et posologie , Cérium/administration et posologie , Diabète expérimental/complications , Cicatrisation de plaie/effets des médicaments et des substances chimiques , Animaux , Antibactériens/composition chimique , Antibactériens/pharmacologie , Lignée cellulaire , Mouvement cellulaire/effets des médicaments et des substances chimiques , Cérium/composition chimique , Cérium/pharmacologie , Collagène/métabolisme , Diabète expérimental/métabolisme , Gélatine/composition chimique , Cellules endothéliales de la veine ombilicale humaine , Humains , Hydrogels , Nanoparticules métalliques , Méthacrylates/composition chimique , Souris , Rats , Streptozocine/effets indésirablesRÉSUMÉ
The effects of nano particles of CuO on voltage-dependent potassium currents were studied in acutely isolated CA1 pyramidal neurons of rat hippocampus using the whole-cell patch-clamp techniques. Nano particles of CuO had small effects on transient outward potassium current (I(A), no statistical significance) and mainly inhibited delayed rectifier potassium current (I(K)) in the concentration of 5 x 10(-5) g/mL. Nano particles of CuO didn't shift the steady-state activation curve of I(K) and I(A) but negatively shifted the inactivation curve of I(K). The effects on inactivation curve of I(A) had no statistical significance. These results suggested that blockades of K+ currents by nano particles of CuO could be preferential for I(k) for the first time. This may interfere with the normal function of nerve cells.