RÉSUMÉ
BACKGROUND: This study investigated the clinical application of the indocyanine green (ICG) fluorescence navigation technique in bile duct identification during laparoscopic common bile duct exploration (LCBDE) for complex hepatolithiasis. METHODS: Eighty patients with complex hepatolithiasis were admitted to our department between January 2022 and June 2023 and randomly divided into control and observation groups. The control group underwent conventional LCBDE, while the observation group underwent LCBDE guided by ICG fluorescence. RESULTS: Intraoperatively, the observation group had shorter operation and search times for the common bile duct (CBD), as well as reduced intraoperative blood loss and fewer complications, such as conversion to laparotomy and various injuries (gastroduodenal, colon, pancreatic, and vascular) than the control group, with statistical significance (P < 0.05). Postoperatively, the observation group had lower rates of postoperative bile leakage, abdominal infection, postoperative hemorrhage, and residual stone than the control group. Additionally, the observation group demonstrated significantly shorter times for resuming flatus, removal of the abdominal drainage tube, and hospitalization than the control group, with statistical significance (P < 0.05). CONCLUSION: ICG fluorescence navigation technology effectively visualizes the bile duct, improves its identification rate, shortens the operation time, prevents biliary tract injury, and reduces the occurrence of complications.
Sujet(s)
Lithiase cholédocienne , Laparoscopie , Lithiase , Maladies du foie , Humains , Lithiase cholédocienne/chirurgie , Conduit cholédoque/chirurgie , Vert indocyanine , Laparoscopie/méthodes , Durée du séjour , Lithiase/chirurgie , Maladies du foie/chirurgie , Études rétrospectivesRÉSUMÉ
In this study, we examined plant C:N:P stoichiometry of herbaceous plants in different sections (stable area, unstable area and deposition area) of the unstable slope on both shade and sunny aspects of dry-hot valley with different soil properties. The results showed that C concentration (320.59 g·kg-1), N concentration (12.15 g·kg-1), and N:P ratio (25.37) of shoot on the unstable slope were significantly higher than those of root, with 254.01 g·kg-1, 6.12 g·kg-1 and 13.43, respectively. The average value of the C:N ratio was significantly higher in root (43.09) than shoot (31.90). The C content and N:P ratio of shoot and root in stable and unstable areas were significantly higher than in deposition area, whereas the N content in unstable area was significantly higher than that in deposition area on the sunny slope. In addition, the N and P contents of shoot and the root P content in deposition area were significantly higher than in stable and unstable areas, whereas the C content of root in stable and unstable areas were significantly higher than in deposition area on the shade slope. Moreover, the shoot growth of plants was mainly limited by P, whereas root growth was mainly limited by N and the limitation gradually increased as the section goes down. Soil water content (SWC) was an important factor controlling the C, N, and P contents change of shoot with the relative influence ratios of 28.8%, 20.8%, and 19.9%, respectively. Soil organic carbon (SOC) had a significant impact on the C and P contents of root with the relative influence ratios of 49.5% and 22.1%. The change of root N content was mainly affected by soil pH (24.3%). Our results revealed that nutrient allocation of plant was significantly affected by slope aspects, sections and soil factors, which were mainly constituted by SWC, SOC, and soil pH.
Sujet(s)
Carbone , Sol , Sol/composition chimique , Plantes , Eau , NutrimentsRÉSUMÉ
Deregulation of Roundabout homolog 1 (Robo1) has been demonstrated to be associated with several types of human cancer, including gastric cancer. However, the detailed role of Robo1 and its regulatory mechanism in gastric cancer remain largely unclear. In the current study, it was demonstrated that the expression of microRNA (miR)29a was frequently reduced in gastric cancer tissues, compared with their matched normal adjacent tissues. Similar results were additionally observed in AGS and SGC7901 human gastric cancer cells. Overexpression of miR29a led to reduced migration and invasion of AGS cells. To explore the targets of miR29a in gastric cancer, bioinformatics analysis was conducted and Robo1 was identified as a putative target of miR29a. Further western blotting and luciferase activity assay data confirmed that miR29a was able to negatively regulate the protein expression of Robo1, through directly binding to the 3'untranslated region of Robo1 mRNA in gastric cancer cells. In addition, it was demonstrated that Robo1 was frequently upregulated in gastric cancer tissues compared with their matched adjacent normal tissues, and a significant inverse correlation was identified between miR29a and Robo1 expression. In addition, knockdown of Robo1 by small interfering RNA markedly inhibited the migratory and invasive capabilities of AGS cells, which the results obtained with overexpression of miR29a. In conclusion, to the best of our knowledge the current study suggested for the first time, that miR29a inhibits migration and invasion in part via direct inhibition of Robo1 in gastric cancer cells. Therefore, Robo1 and miR29a may serve as diagnostic or therapeutic targets for gastric cancer.