RÉSUMÉ
We studied the immunomodulatory and clinical effects of the empirical formula "tiaomian III decoction" on maternal blood blocking antibody deficiency and recurrent spontaneous abortion. Sixty-one patients with blocking antibody deficiency were divided in the experimental group (N = 31), who took tiaomian III decoction, and the control group (N = 30), who received active immunotherapy with paternal lymphocytes; both treatments lasted 3 months. Blocking antibodies, anti-idiotypic antibodies, interleukin, T-lymphocyte subsets, and macrophage colony-stimulating factor (M-CSF) were tested. After treatment, the positive conversion rate reached 87.1 and 86.7% in the experimental and control groups, respectively. After treatment, CD4 levels decreased while CD8 levels increased in both groups. The CD4/CD8 ratio was higher than normal and increased significantly from pre-treatment (P < 0.05). IL-10 and M-CSF levels increased significantly in both groups (P < 0.05). The 1-year conception rates of the experimental and control groups were 58.1 and 46.7%, respectively (P < 0.05). The results show the tiaomian III decoction can increase the positive conversion rate of maternal blocking antibodies and promote the production of IL-10 and M-CSF. Thus, it strengthens the maternal body's protection of the fetus and maintenance of conception. The higher conception rate of the experimental group demonstrates the positive clinic efficacy of the tiaomian III decoction on maternal blood blocking antibody deficiency and recurrent spontaneous abortion.
Sujet(s)
Avortements à répétition/traitement médicamenteux , Médicaments issus de plantes chinoises/usage thérapeutique , Fécondostimulants/usage thérapeutique , Déficits immunitaires/traitement médicamenteux , Facteurs immunologiques/usage thérapeutique , Avortements à répétition/immunologie , Adulte , Anticorps bloquants/sang , Femelle , Humains , Déficits immunitaires/immunologie , Grossesse , Sous-populations de lymphocytes T/effets des médicaments et des substances chimiques , Sous-populations de lymphocytes T/immunologie , Résultat thérapeutiqueRÉSUMÉ
In order to study the toxication of toxoflavin produced by P. cocovenenans to immunocytes and detoxication, the effects of toxoflavin on rabbit blood culture in the presence of PHA and detoxication function of seven drugs towards this system have been observed by 3H-TdR incorporation assay. The more toxoflavin used, the more reduction of cpm value. The results indicated that toxoflavin in 0.5 microgram/ml or more suppressed the growth of cells to even still lower than that of control in which PHA was absent; and when 0.1 microgram/ml of toxoflavin were used, the cpm value began to rise again. The effects of toxoflavin on lymphocyte and other blood cells were studied under the microscope and the naked-eye. The detoxication effects of the seven drugs on toxoflavin were observed and shown that SOD, GSH, Cyt. C and VC were most effective in protecting the lymphocytes from poisoning.
Sujet(s)
Antidotes/pharmacologie , Lymphocytes/effets des médicaments et des substances chimiques , Animaux , Division cellulaire/effets des médicaments et des substances chimiques , Cytochromes de type c/pharmacologie , Glutathion/pharmacologie , Lymphocytes/cytologie , Pseudomonas/métabolisme , Pyrimidinones/isolement et purification , Pyrimidinones/toxicité , Lapins , Superoxide dismutase/pharmacologie , Triazines/isolement et purification , Triazines/toxicitéRÉSUMÉ
Fermented corn flour containing toxoflavin and bongkretic acid produced by P. farinofermentans can cause acute food poisoning, but study on mutagenicity of toxoflavin has not been reported. We studied the mutagenicity of toxoflavin by Tradescantia Paludosa and animal micronucleus tests. The results showed that toxoflavin possesses distinct mutagenic action in both plant and animal cells. Mutagenic action of toxoflavin in cells may have important bearing on food hygiene examination.