Increased superfetation precedes the evolution of advanced degrees of placentotrophy in viviparous fishes of the family Poeciliidae.

The causes and consequences of the evolution of placentotrophy (post-fertilization nutrition of developing embryos of viviparous organisms by means of a maternal placenta) in non-mammalian vertebrates are still not fully understood. In particular, in the fish family Poeciliidae there is an evolutionary link between placentotrophy and superfetation (ability of females to simultaneously bear embryos at distinct developmental stages), with no conclusive evidence for which of these two traits facilitates the evolution of more advanced degrees of the other. Using a robust phylogenetic comparative method based on Ornstein-Uhlenbeck models of adaptive evolution and data from 36 poeciliid species, we detected a clear causality pattern. The evolution of extensive placentotrophy has been facilitated by the preceding evolution of more simultaneous broods. Therefore, placentas became increasingly complex as an adaptive response to evolutionary increases in the degree of superfetation. This finding represents a substantial contribution to our knowledge of the factors that have shaped placental evolution in poeciliid fishes.

Beyond Pairwise Interactions: Multispecies Character Displacement in Mexican Freshwater Fish Communities.

Competition has long been recognized as a central force in shaping evolution, particularly through character displacement. Yet research on character displacement is biased, as it has focused almost exclusively on pairs of interacting species while ignoring multispecies interactions. Communities are seldom so simple that only pairs of species interact, and it is not clear whether inferences from pairwise interactions are sufficient to explain patterns of phenotypes in nature. Here, we test for character displacement in a natural system of freshwater fishes in western Mexico that contains up to four congeneric species of the genus Poeciliopsis. We analyzed body shape differences between populations with different numbers of competitors while accounting for confounding environmental variables. Surprisingly, we found evidence for convergent character displacement in populations of P. prolifica, P. viriosa, and P. latidens. We also found that the convergence in body shape was not consistently in the same direction, meaning that when three or more competitors co-occurred, we did not find more extreme body shapes compared with when there were only two competitors. Instead, when three or more competitors co-occurred, body shape was intermediate between the shape found with a pair of species and the shape found with no competitor present. This intermediate shape suggests that evolution in multispecies communities likely occurs in response to several competitors rather than to simple pairwise interactions. Overall, our results suggest that competition among multiple species is more complex than simple pairwise competitive interactions.

This town ain't big enough for both of us or is it? Spatial co-occurrence between exotic and native species in an urban reserve.

Exotic species pose a threat to most ecosystems because of their potential to establish negative interactions with native biota. However, exotic species can also offer resources to native species, especially within highly modified environments such as urban ecosystems. We studied 17 exotic-native pairs of species with the potential to compete with one another, or in which one of the species could offer resources to the other, in an urban ecological reserve located within Mexico City. We used two-species occupancy models to analyze the potential association between the presence of the exotic species and the spatial distribution of the native species, as well as to assess if these species tend to avoid each other (negative spatial interaction) or to co-occur more often than expected under the hypothesis of independent occurrences (positive spatial interaction). Our results revealed few cases in which the exotic species influenced occupancy of the native species, and these spatial interactions were mainly positive, indicated by the fact that the occupancy of the native species was usually higher when the exotic species was also present. Seven of the eight observed non-independent patterns of co-occurrence were evident during the dry months of the year, when resources become scarce for most species. Our results also demonstrate that the observed patterns of species co-occurrence depend on the distance to the nearest urban structure and the amount of herb, shrub, and tree cover, indicating that these habitat features influence whether native species avoid or co-occur with exotic species. Our study represents an important contribution to the understanding of temporal dynamics in the co-occurrence between exotic and native species within urban ecological reserves.

Superfetation in live-bearing fishes is not always the result of a morphological constraint.

Superfetation is an unusual reproductive strategy that consists of the presence of multiple broods at different developmental stages within a single female. One hypothesis that was proposed to explain its adaptive significance suggests that, in fishes, superfetation is a response to selective pressures that promote a thin and streamlined body shape, such as high-velocity water systems. Superfetation may allow for reduction in ovary size and hence improve streamlining because superfetating females carry few large, full-term embryos at any given time. We tested this morphological constraint hypothesis using reproductive and morphological data from several populations of two viviparous fishes of the family Poeciliidae (Poeciliopsis gracilis and Poeciliopsis infans). We found no evidence to support the morphological constraint hypothesis. In both species the degree of superfetation varied as a function of a complex interaction between source population and female size, and this interpopulation variation was not associated with the velocity of the water current. Contrary to what we expected, females of P. gracilis with more streamlined bodies were observed in rivers where water velocity is slow or moderate. In P. infans the velocity of the water current did not predict variation in body shape. Our results are noteworthy because a previous study which focused on a congeneric species (Poeciliopsis turrubarensis) demonstrated strong support for this hypothesis. However, based on our evidence we conclude that the association among increased superfetation, streamlined morphologies, and fast-flowing environments is not a general rule and that the adaptive value of superfetation may differ among species.

Spatial and temporal variation in superfoetation and related life history traits of two viviparous fishes: Poeciliopsis gracilis and P. infans.

Superfoetation is the ability of females to simultaneously bear multiple broods of embryos at different developmental stages. Most studies on the phylogenetic distribution of superfoetation and on the factors that potentially promote superfoetation ignore variation within species. Here, we studied 11 populations of two species of viviparous fishes of the family Poeciliidae (Poeciliopsis gracilis and Poeciliopsis infans) and document wide variation in superfoetation and in three related life history traits: brood size, individual embryo mass and total reproductive allotment. We found significant differences in the average number of simultaneous broods among populations of P. gracilis but not among populations of P. infans. In addition, we found even greater variation between months within populations for both species, although no specific pattern of temporal variation was evident. Instead of the expected consistency of seasonal differences in superfoetation across populations, we found that large variation among months within seasons and the amount and direction of this monthly variation differed widely between populations. Our results emphasize the importance of including intraspecific variation in superfoetation and other life history traits in studies that aimed at finding general explanations of life history trait evolution.