RÉSUMÉ
Experimental investigation were conducted with the objective to determine a stem cells, capacity to differentiate in adipogenic direction, if they were obtained from adipose tissue. The investigation results have witnessed, that the cells, obtained from adipose tissue, are capable for a tissue-speciphic differentiation in osteogenic, chondrogenic, and, principally--in adipogenic direction, what confirms a multypotent nature of mesenchymal stem cells of adipose tissue. Adipose tissue constitutes an alternative to the bone marrow, as a source of multipotent mesenchymal stem cells, which may be applied in further investigations, concerning determination of their defense possibility for the transplanted autologous adipose tissue from the tissue resorption, made in a lipophiling way.
Sujet(s)
Adipocytes/cytologie , Tissu adipeux/cytologie , Cellules souches mésenchymateuses/cytologie , Cellules souches multipotentes/cytologie , Adipocytes/métabolisme , Tissu adipeux/métabolisme , Agrécanes/génétique , Agrécanes/métabolisme , Phosphatase alcaline/génétique , Phosphatase alcaline/métabolisme , Animaux , Marqueurs biologiques/métabolisme , Différenciation cellulaire , Chondrocytes/cytologie , Chondrocytes/métabolisme , Expression des gènes , Gènes rapporteurs , Protéines à fluorescence verte/génétique , Protéines à fluorescence verte/métabolisme , Immunohistochimie , Cellules souches mésenchymateuses/métabolisme , Souris , Souris transgéniques , Cellules souches multipotentes/métabolisme , Ostéoblastes/cytologie , Ostéoblastes/métabolisme , Culture de cellules primairesRÉSUMÉ
Possibilities of application of the donor cryoconserved cellular material (mesenchymal stem cells, obtained from adipose tissue) for defense of transplanted adipose graft from the tissue resorption were studied. The adipose graft enrichment, using cryoconserved allogenic mesenchymal multipotent stem cells, obtained from adipose tissue, caused activation of destructive-inflammatory processes in adipose graft, the adipocytes survival worsening, and in further--the transplant mass deficiency. We suggest inexpedient to apply in clinical practice the donor cryoconserved stem cells for defense of transplanted adipose tissue from the tissue resorption.
