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1.
World J Otorhinolaryngol Head Neck Surg ; 10(2): 113-120, 2024 Jun.
Article de Anglais | MEDLINE | ID: mdl-38855290

RÉSUMÉ

Objective: This cross-sectional study aimed to determine the epidemiology of olfactory and gustatory dysfunctions related to COVID-19 in China. Methods: This study was conducted by 45 tertiary Grade-A hospitals in China. Online and offline questionnaire data were obtained from patients infected with COVID-19 between December 28, 2022, and February 21, 2023. The collected information included basic demographics, medical history, smoking and drinking history, vaccination history, changes in olfactory and gustatory functions before and after infection, and other postinfection symptoms, as well as the duration and improvement status of olfactory and gustatory disorders. Results: Complete questionnaires were obtained from 35,566 subjects. The overall incidence of olfactory and taste dysfunction was 67.75%. Being female or being a cigarette smoker increased the likelihood of developing olfactory and taste dysfunction. Having received four doses of the vaccine or having good oral health or being a alcohol drinker decreased the risk of such dysfunction. Before infection, the average olfactory and taste VAS scores were 8.41 and 8.51, respectively; after infection, they decreased to 3.69 and 4.29 and recovered to 5.83 and 6.55 by the time of the survey. The median duration of dysosmia and dysgeusia was 15 and 12 days, respectively, with 0.5% of patients having symptoms lasting for more than 28 days. The overall self-reported improvement rate was 59.16%. Recovery was higher in males, never smokers, those who received two or three vaccine doses, and those that had never experienced dental health issues, or chronic accompanying symptoms. Conclusions: The incidence of dysosmia and dysgeusia following infection with the SARS-CoV-2 virus is high in China. Incidence and prognosis are influenced by several factors, including sex, SARS-CoV-2 vaccination, history of head-facial trauma, nasal and oral health status, smoking and drinking history, and the persistence of accompanying symptoms.

2.
World J Gastroenterol ; 30(16): 2209-2219, 2024 Apr 28.
Article de Anglais | MEDLINE | ID: mdl-38690022

RÉSUMÉ

Laryngopharyngeal reflux disease (LPRD) is an inflammatory condition in the laryngopharynx and upper aerodigestive tract mucosa caused by reflux of stomach contents beyond the esophagus. LPRD commonly presents with sym-ptoms such as hoarseness, cough, sore throat, a feeling of throat obstruction, excessive throat mucus. This complex condition is thought to involve both reflux and reflex mechanisms, but a clear understanding of its molecular mechanisms is still lacking. Currently, there is no standardized diagnosis or treatment protocol. Therapeutic strategies for LPRD mainly include lifestyle modifications, proton pump inhibitors and endoscopic surgery. This paper seeks to provide a comprehensive overview of the existing literature regarding the mechanisms, patho-physiology and treatment of LPRD. We also provide an in-depth exploration of the association between LPRD and gastroesophageal reflux disease.


Sujet(s)
Reflux gastro-oesophagien , Reflux laryngopharyngé , Inhibiteurs de la pompe à protons , Humains , Reflux laryngopharyngé/physiopathologie , Reflux laryngopharyngé/diagnostic , Reflux laryngopharyngé/thérapie , Reflux gastro-oesophagien/physiopathologie , Reflux gastro-oesophagien/thérapie , Reflux gastro-oesophagien/diagnostic , Inhibiteurs de la pompe à protons/usage thérapeutique , Résultat thérapeutique , Mode de vie
3.
Int Urol Nephrol ; 55(9): 2249-2255, 2023 Sep.
Article de Anglais | MEDLINE | ID: mdl-36853447

RÉSUMÉ

OBJECTIVE: Acute kidney injury (AKI) seriously affects the health of both pregnant women and fetuses. This study aimed to investigate the clinical characteristics and prognosis of pregnancy-related AKI (PR-AKI). METHODS: This case series study enrolled pregnant women with PR-AKI admitted to the surgical intensive care unit of Xinhua Hospital affiliated to Shanghai Jiaotong University School of Medicine between January 2010 and December 2020. RESULTS: Thirty-one PR-AKI patients were enrolled with a mean age of 29.16 ± 4.97 years. Seventeen pregnant women (54.84%) had complete recovery of renal function, 5 (16.13%) had partial recovery of renal function, 2 (6.45%) patients had no renal function improvement, and 7 (22.58%) died. Among the 31 patients with 35 fetuses, 25 (80.6%) pregnant women had poor fetal outcomes, including 5 cases of stillbirths, 5 neonatal asphyxia, 18 premature births, 10 low birth weight, and 8 deficient birth weight infants. Compared to cases with good fetal outcomes, cases with poor fetal outcomes had significantly shorter gestational weeks (39.26 ± 1.53 vs. 31.62 ± 5.50, P = 0.002), lower platelet count (217.13 ± 122.87 vs. 90.24 ± 84.88, P = 0.005), lower hemoglobin (94.19 ± 13.21 vs. 74.48 ± 20.78, P = 0.036), higher blood urea nitrogen (11.87 ± 4.28 vs. 19.47 ± 10.98, P = 0.013), and higher uric acid (262.41 ± 167.00 vs. 586.87 ± 144.52, P < 0.001). CONCLUSIONS: The maternal renal function of women with PR-AKI might improve after treatment, but occurrence rates of adverse fetal outcomes were still high.


Sujet(s)
Atteinte rénale aigüe , Issue de la grossesse , Nouveau-né , Nourrisson , Grossesse , Femelle , Humains , Jeune adulte , Adulte , Issue de la grossesse/épidémiologie , Chine/épidémiologie , Pronostic , Atteinte rénale aigüe/diagnostic , Atteinte rénale aigüe/étiologie , Atteinte rénale aigüe/épidémiologie , Rein , Études rétrospectives
4.
World Allergy Organ J ; 14(6): 100552, 2021 Jun.
Article de Anglais | MEDLINE | ID: mdl-34178240

RÉSUMÉ

BACKGROUND: A higher compliance with clinical guidelines helps improve treatment outcomes. But the clinical practice of otolaryngologists is not always consistent with guidelines. OBJECTIVE: To describe otolaryngologists' compliance with guidelines about allergic rhinitis (AR) management and identify factors responsible for the discordance between clinical practice and guideline recommendations in China. METHODS: A cross-sectional nationwide survey was designed and conducted via an online platform. Recruitment was done by emailing otolaryngologists registered in the Chinese Society of Otorhinolaryngology-Head and Neck Surgery or by inviting otolaryngologists to scan a Quick Respond (QR) code that linked to the questionnaire at various academic meetings. RESULTS: A total of 2142 otolaryngologists were eligible and completed the survey. Of them, 64.7% had over 10 years work experience and 97.4% had a bachelor's degree or higher. About 18.3% of the participants strictly copied the guideline in clinical practice, while 73.7% used the guideline that had been adjusted according to their clinical experience. Otolaryngologists were most concerned about the efficacy, safety, and minimum age of AR medications, and least concerned about patient preferences. Regarding the use of intranasal steroids (INS), leukotriene receptor antagonists (LTRA), and H1-antihistamines, 86.8%, 55.7% and 51.2% of otolaryngologists complied with the guideline recommendations, respectively. Educational background was a factor affecting the compliance with guidelines and acceptance of INS. CONCLUSION: A vast majority of Chinese otolaryngologists complied with the current Chinese AR guidelines. A difference still existed between the otolaryngologists' real-world and guideline-recommended management. The otolaryngologists should pay more attention to patient preferences. A higher education could improve otolaryngologists' adherence to the guidelines.

5.
Clin Exp Nephrol ; 24(2): 107-118, 2020 Feb.
Article de Anglais | MEDLINE | ID: mdl-31811410

RÉSUMÉ

BACKGROUND: The activation of the nuclear factor-κB (NF-κB) signaling pathway gives rise to inflammation in the pathogenesis of lupus nephritis (LN), with A20 serving as a negative feedback regulator and ubiquitin C­terminal hydrolase L1 (UCH-L1) acting as a downstream target protein. However, their roles in the mechanism of LN remain undetermined. METHODS: In the present study, the expression of A20 and UCH-L1, the activity of NF-κB and ubiquitin-proteasome system (UPS) were measured in MRL/lpr mice and A20 gene silenced podocytes. The severity of podocyte injury and immune complex deposits were detected by transmission electron microscopy. RESULTS: The in vivo experiments revealed that A20 failed to terminate the activation of NF-κB, which was accompanied by UCH-L1 overexpression, ubiquitin accumulation, and glomerular injury in LN mice. Immunosuppression therapy did improve LN progression by attenuating A20 deficiency. In vitro experiments confirmed that tumor necrosis factor-α induced NF-κB activation, which led to UCH-L1 overexpression, UPS impairment, the upregulation of desmin and the downregulation of synaptopodin in A20 gene silenced podocytes. CONCLUSION: Thus, the results of the present study suggest that A20 regulates UCH-L1 expression via the NF-κB signaling pathway and A20 deficiency might play an important role in LN pathogenesis. Therefore, the A20 protein may serve as a promising therapeutic target for LN.


Sujet(s)
Glomérulonéphrite lupique/métabolisme , Podocytes/métabolisme , Protéine-3 induite par le facteur de nécrose tumorale alpha/déficit , Animaux , Complexe antigène-anticorps/ultrastructure , Lignée cellulaire , Modèles animaux de maladie humaine , Femelle , Glomérulonéphrite lupique/génétique , Glomérulonéphrite lupique/immunologie , Glomérulonéphrite lupique/anatomopathologie , Souris de lignée C57BL , Souris de lignée MRL lpr , Facteur de transcription NF-kappa B/métabolisme , Podocytes/immunologie , Podocytes/ultrastructure , Proteasome endopeptidase complex/métabolisme , Transduction du signal , Protéine-3 induite par le facteur de nécrose tumorale alpha/génétique , Ubiquitine/métabolisme , Ubiquitin thiolesterase/métabolisme
6.
Ann Transl Med ; 7(12): 265, 2019 Jun.
Article de Anglais | MEDLINE | ID: mdl-31355232

RÉSUMÉ

BACKGROUND: Type 1 diabetes mellitus (DM) is associated with severe osteoporosis, which is still a great challenge in the clinic. This work aimed to investigate the skeletal effects of FK506 in a rat model of streptozocin induced type 1 DM. METHODS: Rats were divided into three groups: control (CTL), DM rats and DM rats treated with FK506. Dual energy X-ray absorption, micro-computed tomography, bone mechanics and bone histology were used for skeletal analysis. Bone marrow adipocytes infiltrations were detected by oil red O stain and H&E stain. In addition, the protein expression of adipocyte-specific makers (PPAR-γ, C/EBP-αß), osteoblast-specific markers (Runx2, Osterix) and nuclear translocation of ß-catenin in femurs were determined by western blot. RESULTS: In the study, bone mineral density of femurs and lumbar vertebras in diabetic rats were increased after FK506 administration. FK506 treatment resulted in higher cancellous bone volume but had no significant effect on cortical bones in diabetic rats. The ultimate force and work to failure were increased in DM+FK506 group, while they were reduced in the DM group. Compared with the CTL, the infiltration of bone marrow adipocytes was significantly increased in the DM group, which was reduced after the treatment of FK506. Besides, the expression levels of Runx2 and Osterix were up-regulated, and that of PPAR-γ and C/EBP-α were down-regulated in diabetic rats after FK506 treatment. In addition, the nuclear translocation of ß-catenin protein levels were increased in diabetic rats after the treatment of FK506. CONCLUSIONS: Our study indicated that FK506 could alleviate bone loss in diabetic rats. This effects could be due to the results of enhancing osteogenesis and inhibiting adipogenesis, which might be regulated by activation the nuclear translocation of ß-catenin.

7.
J Cell Physiol ; 234(9): 16191-16204, 2019 Sep.
Article de Anglais | MEDLINE | ID: mdl-30805933

RÉSUMÉ

Lupus nephritis (LN), an autoimmune kidney disease caused by systemic lupus erythematosus (SLE), is the inflammation of the kidney. Although the treatment of LN is still a therapeutic challenge for many practitioners, the present study aims to provide a new insight for the treatment and management. The study aims to explore the effect of A20 on LN in relation to the nuclear factor-kappa B (NF-κB) signaling pathway. MRL/lpr mice were used as the LN mouse model. Next, A20, UCH-L1, and NF-κB expression in LN patients and MRL/lpr mice was determined. A20 was upregulated in podocytes to assess biological functions of A20 in LN. Furthermore, to further investigate the pivotal role of the NF-κB pathway in LN, the NF-κB pathway was blocked in podocytes. Next, UCH-L1 was downregulated in MRL/lpr mice to assess biological functions of UCH-L1 in LN. A20 was downregulated, whereas UCH-L1 was upregulated in LN. Overexpressed A20 declined NF-κB, UCH-L1 expression, and the extent of p65 phosphorylation. A20 overexpression or UCH-L1 inhibition increased expression of synaptoporin and nephrin but decreased desmin expression and ubiquitin accumulation level in podocytes. Moreover, A20 overexpression or UCH-L1 inhibition increased the podocyte number but decreased protein level of cleaved caspase-3, podocyte lesion improvement, decreased foot process width, glomerulus basement membrane, and foot process fusion rate. In addition, urine protein, blood urea nitrogen, serum creatinine, and ds-DNA antibody levels decreased with elevated A20 or depleted UCH-L1. Collectively, it could be concluded that A20 protects against podocyte injury in LN via UCH-L1 by inactivating the NF-κB signaling pathway.

9.
Kidney Blood Press Res ; 43(3): 820-835, 2018.
Article de Anglais | MEDLINE | ID: mdl-29843125

RÉSUMÉ

BACKGROUND/AIMS: Nephrolithiasis plagues a great number of patients all over the world. Increasing evidence shows that the extracellular signal-regulated kinase (ERK) signaling pathway and renal tubular epithelial cell (RTEC) dysfunction and attrition are central to the pathogenesis of kidney diseases. Mucin 4 (MUC4) is reported as an activator of ERK signaling pathway in epithelial cells. In this study, using rat models of calcium oxalate (CaOx) nephrolithiasis, the present study aims to define the roles of MUC4 and ERK signaling pathway as contributors to oxidative stress and CaOx crystal formation in RTEC. METHODS: Data sets of nephrolithiasis were searched using GEO database and a heat flow map was drawn. Then MUC4 function was predicted. Wistar rats were prepared for the purpose of model establishment of ethylene glycol and ammonium chloride induced CaOx nephrolithiasis. In order to assess the detailed regulatory mechanism of MUC4 silencing on the ERK signaling pathway and RTEC, we used recombinant plasmid to downregulate MUC4 expression in Wistar rat-based models. Samples from rat urine, serum and kidney tissues were reviewed to identify oxalic acid and calcium contents, BUN, Cr, Ca2+ and P3+ levels, calcium crystal formation in renal tubules and MUC4 positive expression rate. Finally, RT-qPCR, Western blot analysis, and ELISA were employed to access oxidative stress state and CaOx crystal formation in RTEC. RESULTS: Initially, MUC4 was found to have an influence on the process of nephrolithiasis. MUC4 was upregulated in the CaOx nephrolithiasis model rats. We proved that the silencing of MUC4 triggered the inactivation of ERK signaling pathway. Following the silencing of MUC4 or the inhibition of ERK signaling pathway, the oxalic acid and calcium contents in rat urine, BUN, Cr, Ca2+ and P3+ levels in rat serum, p-ERK1/2, MCP-1 and OPN expressions in RTEC and H2O2 and MDA levels in the cultured supernatant were downregulated, but the GSH-Px, CAT and SOD levels in the cultured supernatant were increased. Moreover, MUC4 silencing or ERK signaling pathway inactivation may decrease the formation of CaOx crystals. CONCLUSION: Taken together, silencing of MUC4 can inactivate the ERK signaling pathway and further restrain oxidative stress and CaOx crystal formation in RTEC. Thus, MUC4 represents a potential investigative focus target in nephrolithiasis.


Sujet(s)
Oxalate de calcium/analyse , Cellules épithéliales/métabolisme , Extracellular Signal-Regulated MAP Kinases/métabolisme , Mucine-4/génétique , Néphrolithiase/étiologie , Stress oxydatif/effets des médicaments et des substances chimiques , Animaux , Extinction de l'expression des gènes , Tubules rénaux/anatomopathologie , Rats , Rat Wistar
11.
Article de Anglais | MEDLINE | ID: mdl-27827944

RÉSUMÉ

OBJECTIVE: Asthma is one of the most common chronic diseases and associated with significant morbidity and mortality. However, few data on occupational and environmental risk factors of asthma are available, particularly in Asian adults. Based on a national cross-sectional survey, we assessed the prevalence and risk factors of asthma in Chinese adults. METHODS: A total of 9974 participants aged 15 years and over in seven Chinese cities were selected using a stratified four-stage random sampling. All participants were interviewed face-to-face in their homes using a standardized self-administered questionnaire. Multivariate logistic regression analyses were adopted to determine various risk factors for asthma. RESULTS: The prevalence of self-reported lifetime asthma was 2.46% among the entire adult population, 3.02% among males and 1.93% among females. The prevalence varied by age group, ethnicity, marital status, education, and floor space per person (p < 0.05). After adjusting for socio-demographic variables and smoking, we found independent occupational and environmental determinants of asthma, including a clearance-related job (OR = 2.28, 95%CI: 1.07-4.89), occupational exposure to industrial or occupational poisonous gas (OR = 4.21, 95%CI: 2.43-7.30), having large amounts of carpet in the workplace (OR = 2.61, 95%CI: 1.20-5.69) and using coal for cooking (OR = 2.65, 95%CI: 1.26-5.57). CONCLUSIONS: Asthma is a serious public health problem in China. Our study provides important updated information on the prevalence of asthma and its associated risk factors, which may help us better understand the epidemiology of asthma and prevent this disorder.


Sujet(s)
Asthme/épidémiologie , Exposition environnementale , Facteurs socioéconomiques , Adolescent , Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Asthme/étiologie , Chine/épidémiologie , Villes/épidémiologie , Études transversales , Femelle , Humains , Mâle , Adulte d'âge moyen , Exposition professionnelle , Prévalence , Facteurs de risque , Autorapport , Jeune adulte
12.
Respir Res ; 17(1): 54, 2016 05 17.
Article de Anglais | MEDLINE | ID: mdl-27184027

RÉSUMÉ

BACKGROUND: Chronic rhinosinusitis (CRS) is defined as a condition of inflammation in the paranasal sinus mucosa persisting for more than 12 weeks. We previously reported that the prevalence of CRS was about 8 % in China. Here, we aim to investigate the occupational and environmental risk factors associated with CRS. METHODS: Data were collected from seven Chinese cities: Urumqi, Changchun, Beijing, Wuhan, Chengdu, Huaian and Guangzhou. CRS was diagnosed according to the European Position Paper on Rhinosinusitis and Nasal Polyps (EP(3)OS) document. Participants were asked to complete a standardized questionnaire, which was developed by the Global Allergy and Asthma European Network (GA(2)LEN) project and covered sociodemographic characteristics, CRS-related symptoms and occupational and environmental exposures. We evaluated the association between CRS and various occupational and environmental factors using odds ratios (ORs) and 95 % confidence intervals (95 % CIs). RESULTS: The total study population consisted of 10,633 subjects, 850 (7.99 %) of whom were defined as having CRS according to the EP(3)OS criteria. We found that there were significant associations between occupational and environmental factors and CRS. Specifically, having a clearance-related job, occupational exposure to dust, occupational exposure to poisonous gas, a pet at home or carpet at home or at the workplace were risk factors for CRS. Additionally, the method used to keep warm in winter, the duration of time spent using air conditioning in summer and the frequency of exposure to mouldy or damp environments were significantly different in subjects with and without CRS. CONCLUSIONS: Our data showed that some occupational and environmental exposures are strongly associated with CRS, which aids in understanding the epidemiology of CRS.


Sujet(s)
Pollution de l'air intérieur , Exposition par inhalation/effets indésirables , Polypes du nez/épidémiologie , Maladies professionnelles/épidémiologie , Exposition professionnelle/effets indésirables , Santé au travail , Rhinite/épidémiologie , Sinusite/épidémiologie , Adolescent , Adulte , Loi du khi-deux , Enfant , Enfant d'âge préscolaire , Chine/épidémiologie , Maladie chronique , Études transversales , Femelle , Humains , Nourrisson , Nouveau-né , Modèles logistiques , Mâle , Adulte d'âge moyen , Analyse multifactorielle , Polypes du nez/diagnostic , Maladies professionnelles/diagnostic , Odds ratio , Prévalence , Rhinite/diagnostic , Facteurs de risque , Sinusite/diagnostic , Enquêtes et questionnaires , Jeune adulte
13.
J Pharm Pharmacol ; 68(3): 379-88, 2016 Mar.
Article de Anglais | MEDLINE | ID: mdl-26968978

RÉSUMÉ

OBJECTIVES: Hydrogen sulphide (H2 S) has been found to be involved in cardiovascular diseases, but the exact mechanism has not been clarified. The purpose of this study was to investigate whether sodium hydrogen sulphide (NaHS), the donor of H2 S, can improve diabetic cardiomyopathy by reversing disordered calcium-handling system in sarcoplasmic reticulum (SR). METHODS: Sprague Dawley rats were injected with streptozotocin (STZ, 60 mg/kg, i.p.) to build diabetic model. Treatment groups included: aminoguanidine group (AG, 100 mg/kg, p.o.) and NaHS group (5 mg/kg per day, s.c.). KEY FINDINGS: Cardiac dysfunction and myocardial hypertrophy were found in diabetic model (DM) group, along with increased ROS levels and upregulated mRNA and protein expressions of NADPH p22(phox) , endothelin A receptor (ETA ) and protein kinase Cε (PKCε). Expressions of calcium-handling proteins in SR including FK506-binding proteins (FKBP12.6), sarcoplasmic reticulum Ca(2+) ATPase (SERCA2a) and calsequestrin 2 (CASQ2) were downregulated in DM group, accompanied by elevated concentration of diastolic free calcium in high glucose-incubated cardiomyocytes, indicating of calcium leak. After treated by NaHS, these abnormalities were attenuated significantly. CONCLUSIONS: Exogenous H2 S played a protective role in diabetic cardiomyopathy by inhibiting abnormal calcium-handling system in SR and ET-NADPH oxidase-PKCε pathway.


Sujet(s)
Calcium/métabolisme , Cardiomyopathies diabétiques/traitement médicamenteux , Sulfure d'hydrogène/pharmacologie , Réticulum sarcoplasmique/effets des médicaments et des substances chimiques , Réticulum sarcoplasmique/métabolisme , Animaux , Diabète expérimental/induit chimiquement , Diabète expérimental/traitement médicamenteux , Diabète expérimental/métabolisme , Cardiomyopathies diabétiques/métabolisme , Modèles animaux de maladie humaine , Régulation négative/effets des médicaments et des substances chimiques , Mâle , Myocarde/métabolisme , Myocytes cardiaques/effets des médicaments et des substances chimiques , Myocytes cardiaques/métabolisme , ARN messager/métabolisme , Rats , Rat Sprague-Dawley , Espèces réactives de l'oxygène/métabolisme , Transduction du signal/effets des médicaments et des substances chimiques , Streptozocine/pharmacologie , Sulfures/pharmacologie , Régulation positive/effets des médicaments et des substances chimiques
14.
Adv Mater ; 28(18): 3423-52, 2016 05.
Article de Anglais | MEDLINE | ID: mdl-26996295

RÉSUMÉ

In view of the climate changes caused by the continuously rising levels of atmospheric CO2 , advanced technologies associated with CO2 conversion are highly desirable. In recent decades, electrochemical reduction of CO2 has been extensively studied since it can reduce CO2 to value-added chemicals and fuels. Considering the sluggish reaction kinetics of the CO2 molecule, efficient and robust electrocatalysts are required to promote this conversion reaction. Here, recent progress and opportunities in inorganic heterogeneous electrocatalysts for CO2 reduction are discussed, from the viewpoint of both experimental and computational aspects. Based on elemental composition, the inorganic catalysts presented here are classified into four groups: metals, transition-metal oxides, transition-metal chalcogenides, and carbon-based materials. However, despite encouraging accomplishments made in this area, substantial advances in CO2 electrolysis are still needed to meet the criteria for practical applications. Therefore, in the last part, several promising strategies, including surface engineering, chemical modification, nanostructured catalysts, and composite materials, are proposed to facilitate the future development of CO2 electroreduction.

15.
Cardiovasc Diabetol ; 15: 42, 2016 Mar 05.
Article de Anglais | MEDLINE | ID: mdl-26944557

RÉSUMÉ

BACKGROUND: Previous studies have shown that high glucose (HG) induced endothelial cell (EC) damage via a phenotypic transition of EC. There is increasing evidence suggesting the role of inflammatory cytokines in mediated HG-induced EC damage. However, little is known about the potential role of interleukin-1ß (IL-1ß) in the process. The aim of present study was to investigate whether IL-1ß mediated HG-induced phenotypic transition in human aortic endothelial cells (HAECs) and to determine the possible underlying mechanism. METHODS: Primary HAECs were exposed to normal glucose (NG, 5.5 nM), high glucose (HG,30 nM), IL-1ß (10 ng/ml), HG + IL-1ß (10 ng/ml) and HG + anti-IL-1ß antibodies (1000 ng/ml) or HG + IL-1ß small interfering RNA (siRNA). Pathological changes were investigated using confocal microscopy and electron microscopy. Confocal microscopy was performed to detect the co-expression of CD31 and fibroblast specific protein 1 (FSP1). To study the effect of protein kinase C-ß (PKCß) activation on IL-1ß in HAECs, HAECs were stimulated with 30 nM PMA (PKCß activator) and 0.3 µM PKCß inhibition (LY317615) for 48 h in the NG or HG group. The expressions of PKCß and IL-1ß were detected by RT-PCR and Western blot. And the concentration of IL-1ß in the supernatant of HAECs was measured by ELISA. The expressions of FSP1, a-SMA and CD31 were detected by Western blot. RESULTS: It was shown that the HG resulted in significant increase in the expressions of PKCß and IL-1ß in dose-and time-dependent manners. The HG or exogenous IL-1ß alone inhibited the expression of CD31 and markly increased the expressions of FSP1 and α-SMA. Furthermore, we observed that the HG and IL-1ß synergistically increased FSP1 and a-SMA expressions compared with the HG or IL-1ß alone group (P < 0.05). Confocal microscopy revealed a colocalization of CD31 and FSP1 and that some cells acquired spindle-shaped morphologies and a loss of CD31 staining. Electron microscopy showed that the HG resulted in the increased microfilamentation and a roughened endoplasmic reticulum structure in the cytoplasm. However, the changes above were attenuated by the intervention of anti-IL-1ß antibodies or IL-1ß siRNA (P < 0.05). In addition, the PMA induced the expressions of PKCß and IL-1ß in HAECs. The PKCß activation may mediate the effect of the HG on IL-1ß production, which could be attenuated by the PKCß selective inhibitor (LY317615) (P < 0.05). CONCLUSIONS: Our findings suggested that HG-induced phenotypic transition of HAECs might require IL-ß activation via the PKCß pathway.


Sujet(s)
Aorte/effets des médicaments et des substances chimiques , Cellules endothéliales/effets des médicaments et des substances chimiques , Glucose/pharmacologie , Interleukine-1 bêta/métabolisme , Actines/métabolisme , Aorte/métabolisme , Aorte/ultrastructure , Protéines de liaison au calcium/métabolisme , Cellules cultivées , Relation dose-effet des médicaments , Cellules endothéliales/métabolisme , Cellules endothéliales/ultrastructure , Activation enzymatique , Régulation de l'expression des gènes , Glucose/métabolisme , Humains , Interleukine-1 bêta/génétique , Interleukine-1 bêta/pharmacologie , Phénotype , Antigènes CD31/métabolisme , Protein kinase C beta/antagonistes et inhibiteurs , Protein kinase C beta/métabolisme , Inhibiteurs de protéines kinases/pharmacologie , Interférence par ARN , Protéine S100A4 liant le calcium , Transduction du signal/effets des médicaments et des substances chimiques , Transfection
17.
Yi Chuan ; 35(9): 1095-100, 2013 Sep.
Article de Chinois | MEDLINE | ID: mdl-24400483

RÉSUMÉ

Pro-opiomelancortin (POMC) plays important roles in the regulation of food intake and energy expenditure. The sheep exon 3 of gene POMC was amplified and sequenced by screening the DNA pools to select single nuclear polymorphisms and analyze the association with the growth traits. Two silent SNP mutations (g.273 T/C and g.456 G/A) in Hu sheep were identified. PCR-restriction fragment length polymorphism (RFLP) was used to test the g.273 T/C and the association between the g.273 T/C polymorphism and some growth traits was analyzed in Hu sheep (n = 162) and East Friesian x Hu crossbred sheep (n=130). The results showed that three genotypes, TT, TC and CC, were detected in Hu sheep with the frequencies of 0.469, 0.438 and 0.093, respectively. Two genotypes, TT and TC, were detected in East Friesian x Hu crossbred sheep with the frequencies of 0.754 and 0.246, respectively. The association analysis showed that in Hu sheep the two-month weaning weight, four-month rump height of genotype CC and the four-month body length, cannon circumference of genotype TC were significantly higher than those of genotype TT (P < 0.05); the four- and six-month weight of genotype CC were significantly higher than those of genotypes TT and TC (P < 0.01); the four-month body height and body length of genotype CC were significantly higher than those of genotypes TT (P < 0.01) and TC (P < 0.05); the four-month cannon circumference of CC genotype was significantly higher than that of TT genotype (P < 0.01). In East Friesian x Hu crossbred sheep the two-month weaning weight, four-month weight, body height, body length, chest depth and cannon circumference of genotype TC were significantly higher than those of genotype TT (P < 0.05); the six-month weight of genotype TC was significantly higher than that of genotype CC (P < 0.01). In conclusion, the exon 3 of gene POMC was associated with growth traits, and C allele was beneficial to the increase of body weight and body size traits of sheep, which potentially afford a good foundation for further study on POMC gene as aided breeding markers for growth traits in sheep.


Sujet(s)
Exons , Polymorphisme de nucléotide simple , Pro-opiomélanocortine/génétique , Ovis/croissance et développement , Ovis/génétique , Animaux , Séquence nucléotidique , Mensurations corporelles , Poids , Femelle , Hybridation génétique , Mâle , Données de séquences moléculaires , Caractère quantitatif héréditaire , Ovis/métabolisme
18.
Article de Chinois | MEDLINE | ID: mdl-22932133

RÉSUMÉ

OBJECTIVE: To evaluate the three-year efficacy and safety with standardized dust mite subcutaneous immunotherapy in patients with allergic rhinitis. METHODS: Ninety patients who were diagnosed as allergic to mite by skin prick test and serum IgE were include in the standardized allergen-specific dose-escalation regimen. Nasal symptom score (0-3) were collected before treatment and three years after treatment; VAS (visual analogue scale, 0-10) of all nasal symptoms and drug use score were collected every four months; frequency of local and systemic reactions were recorded in the duration of dose escalation and maintenance. RESULTS: Nasal blocking, sneeze, rhinorrhea and nasal itch were significantly improved after 3 years treatment (before treatment: 2[2;3], 2[2;3], 2[2;3], 2[1;2] ; after treatment: all were 0[0;0]; Z value were -8.310, -8.408, -8.377, -8.287, all P were 0.000). VAS of all nasal symptoms and drug use score decreased dramatically after escalation period (before treatment: 8.00[7.00;8.85], 2.00[1.50;2.00]; after treatment: 1.00[1.00;1.50], 0 [0;0]; Z value were -8.287, -8.248, P value 0.086, 0.744), and maintained afterwards (F value were 2.483, 0.296; P value were 0.086, 0.744). Ninety-eight case times (64.47%) local reactions mainly happened in maintenance period; the frequency of systemic reactions was 2.54%. CONCLUSION: The standardized specific allergen immunotherapy for allergic rhinitis is safe and effective.


Sujet(s)
Allergènes/usage thérapeutique , Désensibilisation immunologique , Rhinite spasmodique apériodique/thérapie , Adolescent , Adulte , Sujet âgé , Allergènes/immunologie , Animaux , Enfant , Enfant d'âge préscolaire , Désensibilisation immunologique/normes , Femelle , Humains , Mâle , Adulte d'âge moyen , Pyroglyphidae/immunologie , Résultat thérapeutique , Jeune adulte
19.
Chin Med J (Engl) ; 125(9): 1603-7, 2012 May.
Article de Anglais | MEDLINE | ID: mdl-22800829

RÉSUMÉ

BACKGROUND: Interleukin-l7 (IL-17), which exerts strong pro-inflammatory effects, has emerged as an important mediator in inflammation-associated cancer. The aim of this study was to clarify the relationship between IL-17 and tumor associated macrophages (TAMs), and the correlation of the microvessel density in the development of laryngeal squamous cell carcinoma (LSCC). METHODS: Histopathological observations and immunohistochemistry staining for IL-17, CD68, and CD34 were performed on 72 specimens (32 cases of LSCC, 20 cases of adjacent tissues of carcinoma as controls, and 20 cases of chronic hypertrophic laryngitis). Double immunohistochemical staining was done to determine which cells expressed IL-17. Real-time quantitative PCR determined the mRNA expression of IL-17. ELISA was used to detect the expression of the serum level of IL-17 in the three groups. RESULTS: The inflammation response had increased in LSCC. Overexpression of IL-17 and CD68 protein were seen in LSCC (P < 0.01). The expression of IL-17 was different between well and poorly differentiated LSCC (P < 0.01). The IL-17 expressing cells were mainly located in macrophages (CD68(+)/IL17(+)) as demonstrated by double immunohistochemical staining. IL-17 expression significantly correlated with high microvessel density (CD34(+)) in LSCC (P < 0.05). Relatively higher mRNA expression levels of IL-17 were seen in LSCC compared to the controls (P < 0.05). The serum expression of IL-17 was similar among the three groups (P > 0.05). CONCLUSION: IL-17 was expressed by TAMs, and IL-17 may significantly correlate to the differentiation and angiogenesis in the development of LSCC.


Sujet(s)
Carcinome épidermoïde/métabolisme , Interleukine-17/métabolisme , Tumeurs du larynx/métabolisme , Macrophages/métabolisme , Adulte , Sujet âgé , Carcinome épidermoïde/génétique , Femelle , Humains , Immunohistochimie , Interleukine-17/génétique , Tumeurs du larynx/génétique , Mâle , Adulte d'âge moyen , Néovascularisation pathologique/génétique , Néovascularisation pathologique/métabolisme , Réaction de polymérisation en chaine en temps réel , Jeune adulte
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