Dry Season Transpiration and Soil Water Dynamics in the Central Amazon.

With current observations and future projections of more intense and frequent droughts in the tropics, understanding the impact that extensive dry periods may have on tree and ecosystem-level transpiration and concurrent carbon uptake has become increasingly important. Here, we investigate paired soil and tree water extraction dynamics in an old-growth upland forest in central Amazonia during the 2018 dry season. Tree water use was assessed via radial patterns of sap flow in eight dominant canopy trees, each a different species with a range in diameter, height, and wood density. Paired multi-sensor soil moisture probes used to quantify volumetric water content dynamics and soil water extraction within the upper 100 cm were installed adjacent to six of those trees. To link depth-specific water extraction patterns to root distribution, fine root biomass was assessed through the soil profile to 235 cm. To scale tree water use to the plot level (stand transpiration), basal area was measured for all trees within a 5 m radius around each soil moisture probe. The sensitivity of tree transpiration to reduced precipitation varied by tree, with some increasing and some decreasing in water use during the dry period. Tree-level water use scaled with sapwood area, from 11 to 190 L per day. Stand level water use, based on multiple plots encompassing sap flow and adjacent trees, varied from ∼1.7 to 3.3 mm per day, increasing linearly with plot basal area. Soil water extraction was dependent on root biomass, which was dense at the surface (i.e., 45% in the upper 5 cm) and declined dramatically with depth. As the dry season progressed and the upper soil dried, soil water extraction shifted to deeper levels and model projections suggest that much of the water used during the month-long dry-down could be extracted from the upper 2-3 m. Results indicate variation in rates of soil water extraction across the research area and, temporally, through the soil profile. These results provide key information on whole-tree contributions to transpiration by canopy trees as water availability changes. In addition, information on simultaneous stand level dynamics of soil water extraction that can inform mechanistic models that project tropical forest response to drought.

Long-Term Lime and Phosphogypsum Amended-Soils Alleviates the Field Drought Effects on Carbon and Antioxidative Metabolism of Maize by Improving Soil Fertility and Root Growth.

Long-term surface application of lime (L) and/or phosphogypsum (PG) in no-till (NT) systems can improve plant growth and physiological and biochemical processes. Although numerous studies have examined the effects of L on biomass and plant growth, comprehensive evaluations of the effects of this practice on net CO2 assimilation, antioxidant enzyme activities and sucrose synthesis are lacking. Accordingly, this study examined the effects of long-term surface applications of L and PG on soil fertility and the resulting impacts on root growth, plant nutrition, photosynthesis, carbon and antioxidant metabolism, and grain yield (GY) of maize established in a dry winter region. At the study site, the last soil amendment occurred in 2016, with the following four treatments: control (no soil amendments), L (13 Mg ha-1), PG (10 Mg ha-1), and L and PG combined (LPG). The long-term effects of surface liming included reduced soil acidity and increased the availability of P, Ca2+, and Mg2+ throughout the soil profile. Combining L with PG strengthened these effects and also increased SO4 2--S. Amendment with LPG increased root development at greater depths and improved maize plant nutrition. These combined effects increased the concentrations of photosynthetic pigments and gas exchange even under low water availability. Furthermore, the activities of Rubisco, sucrose synthase and antioxidative enzymes were improved, thereby reducing oxidative stress. These improvements in the physiological performance of maize plants led to higher GY. Overall, the findings support combining soil amendments as an important strategy to increase soil fertility and ensure crop yield in regions where periods of drought occur during the cultivation cycle.

Contrasting strategies to cope with drought conditions by two tropical forage C4 grasses.

Drought severely limits forage productivity of C4 grasses across the tropics. The avoidance of water deficit by increasing the capacity for water uptake or by controlling water loss are common responses in forage C4 grasses. Napier grass (Pennisetum purpureum) and Brachiaria hybrid cv. Mulato II are tropical C4 grasses used for livestock production due to their reputed resistance to drought conditions. However, there is scant information on the mechanisms used by these grasses to overcome water-limited conditions. Therefore, assessments of cumulative transpired water, shoot growth, leaf rolling, leaf gas exchange, dry mass production and a number of morpho-physiological traits were recorded over a period of 21 days under well-watered or drought conditions. Drought reduced shoot dry mass of both grasses by 35 %, yet each grass exhibited contrasting strategies to cope with water shortage. Napier grass transpired most available water by the end of the drought treatment, whereas a significant amount of water was still available for Mulato II. Napier grass maintained carbon assimilation until the soil was fairly dry, whereas Mulato II restricted water loss by early stomatal closure at relatively wet soil conditions. Our results suggest that Napier grass exhibits a 'water-spending' behaviour that might be targeted to areas with intermittent drought stress, whereas Mulato II displays a 'water-saving' nature that could be directed to areas with longer dry periods.

Root systems and soil microbial biomass under no-tillage system

Some root parameters such as distribution, length, diameter and dry matter are inherent to plant species. Roots can influence microbial population during vegetative cycle through the rhizodeposits and, after senescence, integrating the soil organic matter pool. Since they represent labile substrates, especially regarding nitrogen, they can determine the rate of nutrient availability to the next crop cultivated under no-tillage (NT). The root systems of two crop species: maize (Zea mays L.) cultivar Cargill 909 and soybean [Glycine max (L.) Merr.] cultivar Embrapa 59, were compared in the field, and their influence on spatial distribution of the microbial C and N in a clayey-textured Typic Hapludox cultivated for 22 years under NT, at Tibagi, State of Paraná (PR), Brazil, was determined. Digital image processing and nail-plate techniques were used to evaluate 40 plots of a 80 ´ 50 ´ 3 cm soil profile. It was observed that 36% and 30% of the maize and soybeans roots, respectively, are concentrated in the 0 to 10 cm soil layer. The percent distribution of root dry matter was similar for both crops. The maize roots presented a total of 1,324 kg C ha-1 and 58 kg N ha-1, with higher root dry matter density and more roots in decomposition in the upper soil layer, decreasing with depth. The soybean roots (392 kg C ha-1 and 21 kg N ha-1) showed higher number of thinner roots and higher density per length unity compared to the maize. The maize roots enhanced microbial-C down to deeper soil layers than did the soybean roots. The microbial N presented a better correlation with the concentration of thin active roots and with roots in decomposition or in indefinite shape, possibly because of higher concentration of C and N easily assimilated by soil microorganisms.

Parâmetros radiculares como distribuição, comprimento, diâmetro e matéria seca são inerentes a cada espécie de planta. As raízes podem influenciar a população microbiana durante o ciclo vegetativo através das rizodeposições e, após a senescência, integrando a matéria orgânica do solo. Pelo fato de representarem um substrato lábil, especialmente de nitrogênio, podem ditar o ritmo da disponibilidade de nutrientes na seqüência de culturas sob o sistema de plantio direto (SPD). A comparação dos sistemas radiculares do milho (Zea mays L.), cultivar Cargill 909 e da soja [Glycine max (L.) Merr.], cultivar Embrapa 59, e sua influência sobre a distribuição espacial do C e N microbianos foi feita em um Latossolo Vermelho-Escuro, textura argilosa, cultivado durante 22 anos sob SPD, no município de Tibagi (PR). Técnicas de processamento de imagens e de placa de pregos foram empregadas na avaliação de 40 quadrículas de um perfil de 80 ´ 50 ´ 3 cm, revelando que 36% das raízes de milho e 30% das raízes de soja estão concentradas na camada 0-10 cm do solo. A distribuição percentual no perfil do solo de matéria seca radicular foi semelhante nas duas culturas. O sistema radicular do milho apresentou 1.324 kg C ha-1 e 58 kg N ha-1, com maior densidade por matéria seca e de raízes em vias de decomposição na camada superficial do solo, decrescendo com a profundidade. O sistema radicular da soja (392 kg C ha-1 e 21 kg N ha-1) teve contribuição maior de raízes finas e de densidade por comprimento do que do milho. O efeito das raízes do milho sobre o C microbiano alcançou camadas mais profundas do que o das raízes da soja. O N microbiano apresentou melhor correlação com a concentração de raízes finas ativas e com as raízes em decomposição ou de forma indefinida, provavelmente em função da maior quantidade de C e N de fácil assimilação pelos microrganismos.

Root systems and soil microbial biomass under no-tillage system

Some root parameters such as distribution, length, diameter and dry matter are inherent to plant species. Roots can influence microbial population during vegetative cycle through the rhizodeposits and, after senescence, integrating the soil organic matter pool. Since they represent labile substrates, especially regarding nitrogen, they can determine the rate of nutrient availability to the next crop cultivated under no-tillage (NT). The root systems of two crop species: maize (Zea mays L.) cultivar Cargill 909 and soybean [Glycine max (L.) Merr.] cultivar Embrapa 59, were compared in the field, and their influence on spatial distribution of the microbial C and N in a clayey-textured Typic Hapludox cultivated for 22 years under NT, at Tibagi, State of Paraná (PR), Brazil, was determined. Digital image processing and nail-plate techniques were used to evaluate 40 plots of a 80 ´ 50 ´ 3 cm soil profile. It was observed that 36% and 30% of the maize and soybeans roots, respectively, are concentrated in the 0 to 10 cm soil layer. The percent distribution of root dry matter was similar for both crops. The maize roots presented a total of 1,324 kg C ha-1 and 58 kg N ha-1, with higher root dry matter density and more roots in decomposition in the upper soil layer, decreasing with depth. The soybean roots (392 kg C ha-1 and 21 kg N ha-1) showed higher number of thinner roots and higher density per length unity compared to the maize. The maize roots enhanced microbial-C down to deeper soil layers than did the soybean roots. The microbial N presented a better correlation with the concentration of thin active roots and with roots in decomposition or in indefinite shape, possibly because of higher concentration of C and N easily assimilated by soil microorganisms.

Parâmetros radiculares como distribuição, comprimento, diâmetro e matéria seca são inerentes a cada espécie de planta. As raízes podem influenciar a população microbiana durante o ciclo vegetativo através das rizodeposições e, após a senescência, integrando a matéria orgânica do solo. Pelo fato de representarem um substrato lábil, especialmente de nitrogênio, podem ditar o ritmo da disponibilidade de nutrientes na seqüência de culturas sob o sistema de plantio direto (SPD). A comparação dos sistemas radiculares do milho (Zea mays L.), cultivar Cargill 909 e da soja [Glycine max (L.) Merr.], cultivar Embrapa 59, e sua influência sobre a distribuição espacial do C e N microbianos foi feita em um Latossolo Vermelho-Escuro, textura argilosa, cultivado durante 22 anos sob SPD, no município de Tibagi (PR). Técnicas de processamento de imagens e de placa de pregos foram empregadas na avaliação de 40 quadrículas de um perfil de 80 ´ 50 ´ 3 cm, revelando que 36% das raízes de milho e 30% das raízes de soja estão concentradas na camada 0-10 cm do solo. A distribuição percentual no perfil do solo de matéria seca radicular foi semelhante nas duas culturas. O sistema radicular do milho apresentou 1.324 kg C ha-1 e 58 kg N ha-1, com maior densidade por matéria seca e de raízes em vias de decomposição na camada superficial do solo, decrescendo com a profundidade. O sistema radicular da soja (392 kg C ha-1 e 21 kg N ha-1) teve contribuição maior de raízes finas e de densidade por comprimento do que do milho. O efeito das raízes do milho sobre o C microbiano alcançou camadas mais profundas do que o das raízes da soja. O N microbiano apresentou melhor correlação com a concentração de raízes finas ativas e com as raízes em decomposição ou de forma indefinida, provavelmente em função da maior quantidade de C e N de fácil assimilação pelos microrganismos.

EFEITO DO MÉTODO DE PREPARO DO SOLO NA DISTRIBUIÇÃO RADICULAR DO MILHO (Zea mays, L.) EM TERRA ROXA ESTRUTURADA

An experiment was conducted to determine the effect of two soil tillage methods (subsoiling and non-sobsoiling) on root distribution of corn (Zea mays, L.) in several soil profiles. It was located in Piracicaba, SP, Brazil and the samples were 0,008m³ soil monoliths with corn roots, took from pits with dimension of 1,12 X 0,90 X 1,00m. It was observed a greater root mass and a greater variability on root distribution in the subsoiled as compared with the treatment without subsoiling. However no statical diference was observed among treatments.

Foi realizado um estudo para determinar o efeito de dois sistemas de preparo do solo na distribuição radicular do milho (Zea mays, L.), nas diversas camadas do perfil do solo, em Piracicaba, SP. As amostras foram monólitos de solo de 0,008m³ com raízes de milho, retiradas de trincheiras com 1,12 X 0,90 X 1,00m. Constatou-se que efetivamente ocorreu uma maior massa radicular e maior variabilidade na distribuição de raízes, no preparo do solo subsolado em relação ao solo não subsolado, mas não houve diferença significativa entre os tratamentos.

EFEITO DO MÉTODO DE PREPARO DO SOLO NA DISTRIBUIÇÃO RADICULAR DO MILHO (Zea mays, L.) EM TERRA ROXA ESTRUTURADA

An experiment was conducted to determine the effect of two soil tillage methods (subsoiling and non-sobsoiling) on root distribution of corn (Zea mays, L.) in several soil profiles. It was located in Piracicaba, SP, Brazil and the samples were 0,008m³ soil monoliths with corn roots, took from pits with dimension of 1,12 X 0,90 X 1,00m. It was observed a greater root mass and a greater variability on root distribution in the subsoiled as compared with the treatment without subsoiling. However no statical diference was observed among treatments.

Foi realizado um estudo para determinar o efeito de dois sistemas de preparo do solo na distribuição radicular do milho (Zea mays, L.), nas diversas camadas do perfil do solo, em Piracicaba, SP. As amostras foram monólitos de solo de 0,008m³ com raízes de milho, retiradas de trincheiras com 1,12 X 0,90 X 1,00m. Constatou-se que efetivamente ocorreu uma maior massa radicular e maior variabilidade na distribuição de raízes, no preparo do solo subsolado em relação ao solo não subsolado, mas não houve diferença significativa entre os tratamentos.