Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 20 de 27
Filtrer
1.
J. health med. sci. (Print) ; 7(1): 15-23, ene.-mar. 2021. tab, ilus
Article de Espagnol | LILACS | ID: biblio-1380259

RÉSUMÉ

El carcinoma del conducto salival es un tumor epitelial maligno agresivo, que involucra principalmente a la glándula parótida, con características histológicas semejantes al carcinoma ductal de glándula mamaria. El propósito de este trabajo fue presentar los resultados clínico-patológicos de cinco casos de carcinoma del conducto salival primario de glándula parótida y evaluar la expresión de Ki67. Histológicamente, el carcinoma del conducto salival presentó nidos epiteliales con patrones papilar, sólido y cribiforme, comedonecrosis tanto en la lesión primaria como en los nodos linfoides metastásicos y, además, invasión perineural. Se demostró con Ki 67 una alta proliferación celular en cuatro (80 %) de los cinco casos estudiados. Se concluyó que: el carcinoma del conducto salival es una lesión maligna de mal pronóstico, raramente informado en la literatura odontológica, con características histológicas semejantes a las del carcinoma ductal de alto grado de la mama; la comedonecrosis es un signo específico de esta enfermedad; puede desarrollarse "de novo" o en un adenoma pleomórfico preexistente; su diagnóstico diferencial histopatológico es fundamental para planificar su tratamiento y determinar su pronóstico, a pesar de su tratamiento quirúrgico y radioterapia postoperatoria es un tumor agresivo con alta proliferación celular, infiltración perineural, recurrencias y metástasis.


Salivary duct carcinoma is an aggressive malignant epithelial tumor, primarily involving the parotid gland, with histologic features similar to ductal carcinoma of the breast. The purpose of this work was to report the clinicopathological results of five cases of primary salivary duct carcinoma of the parotid gland and evaluate the expression of Ki67. Histologically, salivary duct carcinoma presented epithelial nests with papillary, solid, and cribriform patterns, with comedonecrosis in both the primary lesion and the metastatic limph nodes, and perineural invasion. A high cell proliferation was demonstrated with Ki67 in four (80 %) of the five cases studied. We concluded that: salivary duct carcinoma is a malignant lesion with a poor prognosis, rarely reported in the dental literature, with histological characteristics similar to those of high-grade ductal carcinoma of the breast; comedonecrosis is a specific sign of this disease; may develop "de novo" or in a pre-existing pleomorphic adenoma; its differential histopathological diagnosis is essential to plan its treatment and determine its prognosis; despite its surgical treatment and postoperative radiotherapy, it is an aggressive tumor with high cell proliferation, perineural infiltration, recurrences and metastasis.


Sujet(s)
Humains , Mâle , Femelle , Adulte d'âge moyen , Sujet âgé , Tumeurs des glandes salivaires/anatomopathologie , Marqueurs biologiques tumoraux/génétique , Carcinome canalaire/anatomopathologie , Tumeurs des glandes salivaires/génétique , Tumeurs des glandes salivaires/thérapie , Immunohistochimie/méthodes , Antigène KI-67 , Carcinome canalaire/génétique , Carcinome canalaire/thérapie
2.
Rev. cuba. obstet. ginecol ; 45(4): e407, oct.-dic. 2019. tab
Article de Espagnol | LILACS, CUMED | ID: biblio-1126711

RÉSUMÉ

RESUMEN Introducción: La sobreexpresión del HER2 en el carcinoma de mama está asociada a un curso clínico adverso y menor sobrevida por lo que resulta necesario determinar las características histopatológicas presentes en el carcinoma de mama infiltrante en las pacientes que lo padecen, con el fin de establecer una terapéutica correcta e individualizada. Objetivos: Determinar las características histopatológicas más frecuentes presentes en el carcinoma de mama con sobreexpresión del gen HER2. Métodos: Estudio observacional, transversal en el período de enero 2010 a diciembre 2012. Se incluyeron 93 pacientes. Se exploraron variables sociodemográficas, clínicas e hstopatológicas. En el análisis estadístico se utlizaron frecuenicas absolutas y relativas. Resultados: La media de edad fue de 55,1 años y el 68,8 por ciento era de piel blanca. La mama más afectada fue la derecha con el 45,2 por ciento y la localización más frecuente el cuadrante supero-externo con el 26,9 por ciento. El carcinoma ductal invasivo representó el 71 por ciento del total. El grado nuclear 3 se encontró en 81,7 por ciento casos y la necrosis tumoral 88,2 por ciento. Los receptores estrogénicos fueron positivos en 50,5 por ciento y los receptores de progesterona fueron negativos en 59,1 por ciento. El análisis histológico del cáncer de mama será de utilidad para individualizar la terapia de cada una de las pacientes. Conclusiones: Es importante continuar la investigación con la incorporación de otras variables de interés, tales como, respuesta al tratamiento y supervivencia, además de la prueba de hibridización in situ detectada por fluorescencia en las pacientes del universo en las que se constató HER2 2+ para definir su estatus y orientar mejor su tratamiento(AU)


ABSTRACT Introduction: HER2 overexpression in breast carcinoma is associated with the adverse clinical course and shorter survival, so it is necessary to determine the histopathological characteristics present in infiltrating breast carcinoma in patients with it, in order to establish correct and individualized therapy. Objectives: To determine the histopathological characteristics that are most frequently present in breast carcinoma with overexpression of HER2 gene in the studied patients. Methods: Observational, cross-sectional study from January 2010 to December 2012. Nine three patients were included. Sociodemographic, clinical, and pathological variables were explored. Absolute and relative frequencies were used in the statistical analysis. Results: The mean age was 55.1 years; 68.8 percent were white skinned. The most affected breast was the right one in 45.2 percent and the most frequent location was the upper-external quadrant in 26.9 percent. Invasive ductal carcinoma represented 71 percent of the total. Nuclear grade 3 was found in 81.7 percent cases and tumor necrosis 88.2 percent. Estrogen receptors were positive in 50.5 percent and progesterone receptors were negative in 59.1 percent. Histological analysis of breast cancer will be useful to individualize the therapy of each patient. Conclusions: It is important to continue the investigation with the incorporation of other variables of interest, such as response to treatment and survival, in addition to the in situ hybridization test detected by fluorescence in the patients of the universe in which HER2 2+ was found to define the status and better guide the treatment(AU)


Sujet(s)
Humains , Femelle , Adulte d'âge moyen , Tumeurs du sein/diagnostic , Tumeurs du sein/anatomopathologie , Carcinome canalaire/anatomopathologie , Survie , Études transversales , Études observationnelles comme sujet
3.
Sci Rep ; 9(1): 2521, 2019 02 21.
Article de Anglais | MEDLINE | ID: mdl-30792473

RÉSUMÉ

Breast cancer is a group of multigenic diseases. It is the most common cancer diagnosed among women worldwide and is often treated with tamoxifen. Tamoxifen is catalysed by cytochrome P450 2D6 (CYP2D6), and inter-individual variations in the enzyme due to single nucleotide polymorphisms (SNPs) could alter enzyme activity. We evaluated SNPs in patients from Colombia in South America who were receiving tamoxifen treatment for breast cancer. Allelic diversity in the CYP2D6 gene was found in the studied population, with two patients displaying the poor-metaboliser phenotype. Molecular dynamics and trajectory analyses were performed for CYP2D6 from these two patients, comparing it with the common allelic form (CYP2D6*1). Although we found no significant structural change in the protein, its dynamics differ significantly from those of CYP2D6*1, the effect of such differential dynamics resulting in an inefficient enzyme with serious implications for tamoxifen-treated patients, increasing the risk of disease relapse and ineffective treatment.


Sujet(s)
Tumeurs du sein/traitement médicamenteux , Carcinome canalaire/traitement médicamenteux , Cytochrome P-450 CYP2D6/génétique , Tamoxifène/administration et posologie , Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Antinéoplasiques hormonaux/administration et posologie , Antinéoplasiques hormonaux/métabolisme , Tumeurs du sein/génétique , Tumeurs du sein/métabolisme , Tumeurs du sein/anatomopathologie , Carcinome canalaire/génétique , Carcinome canalaire/métabolisme , Carcinome canalaire/anatomopathologie , Traitement médicamenteux adjuvant , Cytochrome P-450 CYP2D6/métabolisme , Femelle , Génotype , Humains , Inactivation métabolique/génétique , Adulte d'âge moyen , Variants pharmacogénomiques/génétique , Phénotype , Polymorphisme de nucléotide simple/génétique , Tamoxifène/effets indésirables , Tamoxifène/métabolisme
4.
Technol Cancer Res Treat ; 17: 1533033818794939, 2018 01 01.
Article de Anglais | MEDLINE | ID: mdl-30157721

RÉSUMÉ

Breast cancer represents a rising problem concerning public health worldwide. Current efforts are aimed to the development of new minimally invasive and conservative treatment procedures for this disease. A treatment approach for invasive breast ductal carcinoma could be based on electroporation. Hence, in order to determine the effectiveness of electrochemotherapy in the treatment of this disease, 12 electrode models were investigated on realistic patient-specific computational breast models of 3 patients diagnosed by Digital Breast Tomosynthesis imaging. The electrode models exhibit 4, 5, and 6 needles arranged in 4 geometric configurations (delta, diamond, and star) and 3 different needle spacing resulting in a total of 12 needle-electrode arrays. Electric field distribution in the tumors and a surrounding safety margin of 1 cm around the tumor edge is computed using the finite element method. Efficiency of the electrode arrays was determined hierarchically based on (1) percentage of tumor volume reversibly electroporated, (2) percentage of tumor volume irreversibly electroporated, (3) percentage of treated safety margin volume, (4) minimal invasiveness, that is, minimal number of electrodes used, (5) minimal activated electrode pairs, and (6) minimal electric current. Results show that 3 electrode arrays (4 needle-delta, 5 needle-diamond, and 6 needle-star) with fixed-geometry configuration could be used in the treatment with electrochemotherapy of invasive breast ductal carcinomas ranging from 1 to 5 cm3 along with a surrounding safety margin of 1 cm.


Sujet(s)
Tumeurs du sein/traitement médicamenteux , Carcinome canalaire/traitement médicamenteux , Électrochimiothérapie , Sujet âgé , Tumeurs du sein/imagerie diagnostique , Tumeurs du sein/anatomopathologie , Carcinome canalaire/imagerie diagnostique , Carcinome canalaire/anatomopathologie , Électrodes , Femelle , Analyse des éléments finis , Humains , Adulte d'âge moyen , Modèles biologiques , Aiguilles , Fantômes en imagerie
5.
Rev Col Bras Cir ; 44(2): 163-170, 2017.
Article de Anglais, Portugais | MEDLINE | ID: mdl-28658335

RÉSUMÉ

Objective: to analyze the relation of anatomopathological features and axillary involvement in cases of invasive ductal carcinoma. Methods: this is a cross-sectional study of 220 breast cancer patients submitted to radical mastectomy or quadrantectomy with axilar emptying, from the Mastology Service of the Assis Chateaubriand Maternity School, Ceará, Brazil. We submitted the tumors to histological processing and determined the histological (HG), tubular (TG) and nuclear (NG) grades, and the mitotic index (MI) by the classification of Scarff-Bloom-Richadson, verified the presence of angiolymphatic invasion (AI) and measured the largest tumor diameter (TD). We then correlated these variables with the presence of axillary metastases. Results: the mean patients'age was 56.81 years ± 13.28. Tumor size ranged from 0.13 to 22 cm, with an average of 2.23cm ± 2.79. HG3, TG3 and NG3 prevailed, respectively 107 (48.6%), 160 (72.7%) and 107 (48.6%). Mitotic indexes 1, 2 and 3 presented a homogeneous distribution, respectively 82 (37.2%), 68 (31%) and 70 (31.8%). We observed no relation between the HG, TG and NG with the occurrence of axillary metastases (p=0.07, p=0.22 and p=0.21, respectively). Mitotic indices 2 and 3 were related with the occurrence of axillary metastases (p=0.03). Tumors larger than 2cm and cases that presented angiolymphatic invasion had a higher index of axillary metastases (p=0.0003 and p<0.0001). Conclusion: elevated mitotic indexes, tumors with a diameter greater than 2cm and the presence of angiolymphatic invasion were individuallyassociatedwith the occurrence of axillary metastases.


Objetivo: analisar a relação das características anatomopatológicas com o comprometimento axilar em casos de carcinoma ductal invasor. Métodos: estudo transversal de 220 pacientes com câncer de mama, submetidas à mastectomia radical ou quadrantectomia com esvaziamento axilar, oriundos do Serviço de Mastologia da Maternidade Escola Assis Chateaubriand, Ceará, Brasil. Os tumores foram submetidos a processamento histológico e, em seguida, foram determinados os graus histológico (GH), tubular (GT), nuclear (GN), índice mitótico (IM) pela classificação de Scarff-Bloom-Richadson, verificada a presença de invasão angiolinfática (IA) e mensurado o maior diâmetro do tumor (DT). Tais variáveis foram correlacionadas com a presença de metástases axilares. Resultados: a média de idade das pacientes foi 56,81 anos ± 13,28. O tamanho do tumor variou de 0,13 a 22 cm, com média de 2,23cm ± 2,79. Os GH3, GT3 e GN3 prevaleceram: n=107 (48,6%), n=160 (72,7%) e n=107 (48,6%), respectivamente. Os índices mitóticos 1, 2 e 3 apresentaram distribuição homogênea: n=82 (37,2%), n=68 (31%) e n=70 (31,8%), respectivamente. Não foi evidenciada relação do GH, GT e GN com a ocorrência de metástases axilares (p=0,07; p=0,22 e p=0,21). Índices mitóticos 2 e 3 apresentaram relação com a o ocorrência de metástases axilares (p=0,03). Tumores maiores do que 2cm e casos com invasão angiolinfática apresentaram maior índice de metástases axilares (p=0,0003 e p<0,0001). Conclusão: índices mitóticos elevados, tumores com diâmetro maior do que 2cm e presença de invasão angiolinfática apresentaram isoladamente relação com a ocorrência de metástases axilares.


Sujet(s)
Aisselle , Tumeurs du sein/anatomopathologie , Carcinome canalaire/anatomopathologie , Carcinome canalaire/secondaire , Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Études transversales , Femelle , Humains , Adulte d'âge moyen , Invasion tumorale
6.
Rev. Col. Bras. Cir ; 44(2): 163-170, Mar.-Apr. 2017. tab, graf
Article de Anglais | LILACS | ID: biblio-842661

RÉSUMÉ

ABSTRACT Objective: to analyze the relation of anatomopathological features and axillary involvement in cases of invasive ductal carcinoma. Methods: this is a cross-sectional study of 220 breast cancer patients submitted to radical mastectomy or quadrantectomy with axilar emptying, from the Mastology Service of the Assis Chateaubriand Maternity School, Ceará, Brazil. We submitted the tumors to histological processing and determined the histological (HG), tubular (TG) and nuclear (NG) grades, and the mitotic index (MI) by the classification of Scarff-Bloom-Richadson, verified the presence of angiolymphatic invasion (AI) and measured the largest tumor diameter (TD). We then correlated these variables with the presence of axillary metastases. Results: the mean patients'age was 56.81 years ± 13.28. Tumor size ranged from 0.13 to 22 cm, with an average of 2.23cm ± 2.79. HG3, TG3 and NG3 prevailed, respectively 107 (48.6%), 160 (72.7%) and 107 (48.6%). Mitotic indexes 1, 2 and 3 presented a homogeneous distribution, respectively 82 (37.2%), 68 (31%) and 70 (31.8%). We observed no relation between the HG, TG and NG with the occurrence of axillary metastases (p=0.07, p=0.22 and p=0.21, respectively). Mitotic indices 2 and 3 were related with the occurrence of axillary metastases (p=0.03). Tumors larger than 2cm and cases that presented angiolymphatic invasion had a higher index of axillary metastases (p=0.0003 and p<0.0001). Conclusion: elevated mitotic indexes, tumors with a diameter greater than 2cm and the presence of angiolymphatic invasion were individuallyassociatedwith the occurrence of axillary metastases.


RESUMO Objetivo: analisar a relação das características anatomopatológicas com o comprometimento axilar em casos de carcinoma ductal invasor. Métodos: estudo transversal de 220 pacientes com câncer de mama, submetidas à mastectomia radical ou quadrantectomia com esvaziamento axilar, oriundos do Serviço de Mastologia da Maternidade Escola Assis Chateaubriand, Ceará, Brasil. Os tumores foram submetidos a processamento histológico e, em seguida, foram determinados os graus histológico (GH), tubular (GT), nuclear (GN), índice mitótico (IM) pela classificação de Scarff-Bloom-Richadson, verificada a presença de invasão angiolinfática (IA) e mensurado o maior diâmetro do tumor (DT). Tais variáveis foram correlacionadas com a presença de metástases axilares. Resultados: a média de idade das pacientes foi 56,81 anos ± 13,28. O tamanho do tumor variou de 0,13 a 22 cm, com média de 2,23cm ± 2,79. Os GH3, GT3 e GN3 prevaleceram: n=107 (48,6%), n=160 (72,7%) e n=107 (48,6%), respectivamente. Os índices mitóticos 1, 2 e 3 apresentaram distribuição homogênea: n=82 (37,2%), n=68 (31%) e n=70 (31,8%), respectivamente. Não foi evidenciada relação do GH, GT e GN com a ocorrência de metástases axilares (p=0,07; p=0,22 e p=0,21). Índices mitóticos 2 e 3 apresentaram relação com a o ocorrência de metástases axilares (p=0,03). Tumores maiores do que 2cm e casos com invasão angiolinfática apresentaram maior índice de metástases axilares (p=0,0003 e p<0,0001). Conclusão: índices mitóticos elevados, tumores com diâmetro maior do que 2cm e presença de invasão angiolinfática apresentaram isoladamente relação com a ocorrência de metástases axilares.


Sujet(s)
Humains , Femelle , Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Aisselle , Tumeurs du sein/anatomopathologie , Carcinome canalaire/anatomopathologie , Carcinome canalaire/secondaire , Études transversales , Adulte d'âge moyen , Invasion tumorale
7.
An. bras. dermatol ; An. bras. dermatol;91(6): 799-802, Nov.-Dec. 2016. tab, graf
Article de Anglais | LILACS | ID: biblio-837989

RÉSUMÉ

Abstract Squamoid eccrine ductal carcinoma is an eccrine carcinoma subtype, and only twelve cases have been reported until now. It is a rare tumor and its histopathological diagnosis is difficult. Almost half of patients are misdiagnosed as squamous cell carcinoma by the incisional biopsy. We report the thirteenth case of squamoid eccrine ductal carcinoma. Female patient, 72 years old, in the last 6 months presenting erythematous, keratotic and ulcerated papules on the nose. The incisional biopsy diagnosed squamoid eccrine ductal carcinoma. After excision, histopathology revealed positive margins. A wideningmargins surgery and grafting were performed, which again resulted in positive margins. The patient was then referred for radiotherapy. After 25 sessions, the injury reappeared. After another surgery, although the intraoperative biopsy showed free surgical margins, the product of resection revealed persistent lesion. Distinction between squamoid eccrine ductal carcinoma and squamous cell carcinoma is important because of the more aggressive nature of the first, which requires wider margins surgery to avoid recurrence.


Sujet(s)
Humains , Sujet âgé , Tumeurs des glandes sudoripares/anatomopathologie , Carcinome canalaire/anatomopathologie , Glandes eccrines/anatomopathologie , Tumeurs des glandes sudoripares/thérapie , Biopsie , Carcinome épidermoïde/anatomopathologie , Carcinome épidermoïde/thérapie , Nez/anatomopathologie , Carcinome canalaire/thérapie , Récidive tumorale locale
8.
An Bras Dermatol ; 91(6): 799-802, 2016.
Article de Anglais | MEDLINE | ID: mdl-28099603

RÉSUMÉ

Squamoid eccrine ductal carcinoma is an eccrine carcinoma subtype, and only twelve cases have been reported until now. It is a rare tumor and its histopathological diagnosis is difficult. Almost half of patients are misdiagnosed as squamous cell carcinoma by the incisional biopsy. We report the thirteenth case of squamoid eccrine ductal carcinoma. Female patient, 72 years old, in the last 6 months presenting erythematous, keratotic and ulcerated papules on the nose. The incisional biopsy diagnosed squamoid eccrine ductal carcinoma. After excision, histopathology revealed positive margins. A wideningmargins surgery and grafting were performed, which again resulted in positive margins. The patient was then referred for radiotherapy. After 25 sessions, the injury reappeared. After another surgery, although the intraoperative biopsy showed free surgical margins, the product of resection revealed persistent lesion. Distinction between squamoid eccrine ductal carcinoma and squamous cell carcinoma is important because of the more aggressive nature of the first, which requires wider margins surgery to avoid recurrence.


Sujet(s)
Carcinome canalaire/anatomopathologie , Glandes eccrines/anatomopathologie , Tumeurs des glandes sudoripares/anatomopathologie , Sujet âgé , Biopsie , Carcinome canalaire/thérapie , Carcinome épidermoïde/anatomopathologie , Carcinome épidermoïde/thérapie , Femelle , Humains , Récidive tumorale locale , Nez/anatomopathologie , Tumeurs des glandes sudoripares/thérapie
9.
Rev. bras. cir. plást ; 31(2): 252-256, 2016. ilus
Article de Anglais, Portugais | LILACS | ID: biblio-1570

RÉSUMÉ

As opções reconstrutivas para defeitos da parede anterior do tórax podem ser desafiadoras especialmente quando o defeito é extenso e sujeito a radioterapia pré-operatória. Apresenta-se caso de paciente com carcinoma ductal invasivo de mama não tratado que realizou radioterapia e quimioterapia incompleta no pré-operatória. O exame patológico revelou carcinoma ductal invasivo de 11,5 cm, grau III, com invasão linfovascular e linfonodos axilares positivos (20/20). Após a mastectomia, defeitos estendidos seguido de longa excisão de pele mediram 25 x 20 cm, sendo esses cobertos imediatamente com retalhos em abdominoplastia reversa tensionada. Trata-se do primeiro caso relatado de excisão larga de mastectomia reconstruída exclusivamente com retalhos avançados em abdominoplastia reversa e alta tensão progressiva de pontos de adesão demostrando que nos pacientes selecionados, a pele abdominal pode ser avançada superiormente com segurança e facilidade para atingir a área superior do tórax e cobrir a área com defeito significante.


Reconstructive options for anterior chest wall defects can be challenging especially when the defect is large and has been subject for preoperative radiotherapy. We report a case of a patient with a neglected large invasive ductal carcinoma of the breast who had received incomplete preoperative radiotherapy and chemotherapy. The pathology examination revealed an 11.5 cm invasive ductal carcinoma, grade III, with lymphovascular invasion and positive axillary lymph nodes (20/20). The post mastectomy large defect following wide skin excision measured 25 x 20 cm and it was immediately covered with a tensioned reverse abdominoplasty flap. To our knowledge, this is the first case reported of a wide skin excision mastectomy reconstructed solely with a reverse abdominoplasty advancement flap and progressive high-tension with quilting sutures demonstrating that, in the selected patients, abdominal skin can be safely and easily advanced superiorly to reach the upper chest area and cover an area of significant defect.


Sujet(s)
Humains , Femelle , Adulte d'âge moyen , Histoire du 21ème siècle , Lambeaux chirurgicaux , Muscles abdominaux , 33584 , Paroi thoracique , Carcinome canalaire , Diffusion des innovations , Abdomen , Mastectomie , Lambeaux chirurgicaux/chirurgie , Muscles abdominaux/chirurgie , Muscles abdominaux/anatomopathologie , 33584/méthodes , Paroi thoracique/chirurgie , Paroi thoracique/anatomopathologie , Carcinome canalaire/chirurgie , Carcinome canalaire/anatomopathologie , Abdomen/chirurgie , Abdomen/anatomopathologie , Tumeurs de l'abdomen , Mastectomie/méthodes , Tumeurs de l'abdomen/chirurgie , Tumeurs de l'abdomen/complications
10.
Genet Mol Res ; 14(2): 5417-26, 2015 May 22.
Article de Anglais | MEDLINE | ID: mdl-26125737

RÉSUMÉ

The aims of this study were to investigate the expression of the H3K4 demethylase, jumonji AT-rich interactive domain 1B (JARID1B/KDM5B) and of p16 (multiple tumor suppressor gene MTS1) in breast cancer tissue and determine its clinicopathological significance. JARID1B/KDM5B and P16 protein expression in 176 resected breast cancer specimens and adjacent normal breast tissue was detected by the streptavidin-peroxidase (S-P) immunohistochemical method. The TNM staging grade was assigned according to the World Health Organization (2012) breast classification system. The positive staining rate of JARID1B/KDM5B and p16 protein in cancer tissue was 74.43 and 35.8%, respectively. JARID1B/KDM5B protein expression was positively associated with T grade, Bloom and Richardson (B&R) score and axillary lymph node metastasis (P < 0.05). p16 protein expression was negatively associated with T grade, B&R score, and axillary lymph node metastasis (P < 0.05). JARID1B/KDM5B and p16 protein expression in breast cancer and adjacent normal breast tissue were negatively correlated (r = -0.303, P < 0.001). The data demonstrated that protein expression of p16 and JARID1B/KDM5B is negatively correlated in invasive ductal carcinoma of the breast.


Sujet(s)
Tumeurs du sein/génétique , Carcinome canalaire/génétique , Inhibiteur p16 de kinase cycline-dépendante/biosynthèse , Jumonji Domain-Containing Histone Demethylases/biosynthèse , Protéines nucléaires/biosynthèse , Protéines de répression/biosynthèse , Adulte , Sujet âgé , Tumeurs du sein/anatomopathologie , Carcinome canalaire/anatomopathologie , Inhibiteur p16 de kinase cycline-dépendante/génétique , Femelle , Régulation de l'expression des gènes tumoraux , Humains , Jumonji Domain-Containing Histone Demethylases/génétique , Métastase lymphatique , Adulte d'âge moyen , Invasion tumorale/génétique , Invasion tumorale/anatomopathologie , Stadification tumorale , Protéines nucléaires/génétique , Protéines de répression/génétique
11.
Appl Immunohistochem Mol Morphol ; 23(1): 19-25, 2015 Jan.
Article de Anglais | MEDLINE | ID: mdl-25153498

RÉSUMÉ

Liver metastasis is common for breast cancer. In breast cancer patients, differentiation between metastasis and intrahepatic cholangiocarcinoma (ICC) is crucial but not always possible, especially when estrogen receptor (ER) is negative. Villin and mammaglobin are gastrointestinal and breast markers, respectively, but were considered not useful in cholangiocarcinoma. The aim of this study was to reevaluate these 2 markers in the differential diagnosis between breast ductal carcinoma and ICC. Fifty-two cases of breast ductal carcinoma (including ER positive and negative, in situ, and invasive carcinomas), 27 cases of ICC, and 19 cases of extrahepatic bile duct adenocarcinoma were retrieved. Immunohistochemical staining for villin and mammaglobin was performed. The results showed that villin was negative in all breast cancers with a specificity of 100%, and positive in all ICCs with a sensitivity of 100%. Its sensitivity in extrahepatic bile duct adenocarcinoma was 63.2%. Mammaglobin was positive in a smaller percentage of breast cancers with no relationship to ER status. Discordant mammaglobin expression among matched invasive, in situ, and metastatic carcinomas of the same patient was common. Mammaglobin was negative in all ICCs with a specificity of 100%, when care was taken not to misinterpret patchy staining in adjacent normal hepatocytes as positive. In conclusion, the data showed that villin expression was highly accurate in the differential diagnosis between ICC and breast ductal carcinoma, which was particularly useful in ER-negative cases. Mammaglobin may be useful when it is positive.


Sujet(s)
Adénocarcinome/diagnostic , Tumeurs des canaux biliaires/diagnostic , Tumeurs du sein/diagnostic , Carcinome canalaire/diagnostic , Cholangiocarcinome/diagnostic , Tumeurs du foie/diagnostic , Mammaglobine A/métabolisme , Protéines des microfilaments/métabolisme , Adénocarcinome/anatomopathologie , Tumeurs des canaux biliaires/anatomopathologie , Conduits biliaires intrahépatiques/anatomopathologie , Marqueurs biologiques tumoraux/métabolisme , Tumeurs du sein/anatomopathologie , Carcinome canalaire/anatomopathologie , Cholangiocarcinome/anatomopathologie , Diagnostic différentiel , Femelle , Humains , Immunohistochimie , Tumeurs du foie/anatomopathologie , Récepteurs des oestrogènes/métabolisme , Sensibilité et spécificité
13.
Eur J Immunol ; 43(6): 1518-28, 2013 Jun.
Article de Anglais | MEDLINE | ID: mdl-23529839

RÉSUMÉ

Breast cancer is a leading cause of neoplasia-associated death in women worldwide. Regulatory T (Treg) and Th17 cells are enriched within some tumors, but the role these cells play in invasive ductal carcinoma (IDC) of the breast is unknown. We show that CD25(+) CD4(+) T cells from PBMCs and tumor express high levels of Foxp3, GITR, CTLA-4, and CD103, indicating that tumor-infiltrating Treg cells are functional and possibly recruited by CCL22. Additionally, we observed upregulation of Th17-related molecules (IL-17A, RORC, and CCR6) and IL-17A produced by tumor-infiltrating CD4(+) and CD8(+) T lymphocytes. The angiogenic factors CXCL8, MMP-2, MMP-9, and vascular endothelial growth factor detected within the tumor are possibly induced by IL-17 and indicative of poor disease prognosis. Treg and Th17 cells were synchronically increased in IDC patients, with positive correlation between Foxp3, IL-17A, and RORC expression, and associated with tumor aggressiveness. Therefore, Treg and Th17 cells can affect disease progression by Treg-cell-mediated suppression of the effector T-cell response, as indicated by a decrease in the proliferation of T cells isolated from PBMCs of IDC patients and induction of angiogenic factors by IL-17-producing Th17. The understanding of regulation of the Treg/Th17 axis may result in novel perspectives for the control of invasive tumors.


Sujet(s)
Tumeurs du sein/immunologie , Carcinome canalaire/immunologie , Interleukine-17/métabolisme , Lymphocytes T régulateurs/immunologie , Cellules Th17/immunologie , Antigènes CD/métabolisme , Tumeurs du sein/anatomopathologie , Carcinome canalaire/anatomopathologie , Prolifération cellulaire , Transformation cellulaire néoplasique/immunologie , Chimiokine CCL22/métabolisme , Femelle , Facteurs de transcription Forkhead/métabolisme , Protéine associée au récepteur du TNF induit par les corticoïdes/métabolisme , Humains , Interleukine-17/génétique , Invasion tumorale , Membre-3 du groupe F de la sous-famille-1 de récepteurs nucléaires/métabolisme , Récepteurs CCR6/métabolisme , Cellules cancéreuses en culture , Régulation positive
14.
Appl Immunohistochem Mol Morphol ; 21(1): 1-12, 2013 Jan.
Article de Anglais | MEDLINE | ID: mdl-22595945

RÉSUMÉ

The distinction between classic lobular and ductal carcinoma, both in situ and invasive, has important therapeutic and management implications. Most ductal and lobular carcinomas are distinguished readily on hematoxylin-eosin-stained sections because of distinct histomorphologic features. In cases with ambiguous morphologic features, however, categorization in one or another type can be a challenge. Several immunohistochemical markers, including epithelial cadherin, p120, ß-catenin, and low-molecular-weight and high-molecular-weight cytokeratins among others, have been introduced to help better discriminate between lobular neoplasia and ductal carcinoma. In this critical review of the literature, we comment about the usefulness and the limitations of these markers to improve the accuracy in the differential diagnosis of breast pathology.


Sujet(s)
Tumeurs du sein/diagnostic , Carcinome canalaire/diagnostic , Carcinome lobulaire/diagnostic , Immunohistochimie/méthodes , Marqueurs biologiques tumoraux/immunologie , Marqueurs biologiques tumoraux/métabolisme , Tumeurs du sein/anatomopathologie , Cadhérines/immunologie , Cadhérines/métabolisme , Carcinome canalaire/anatomopathologie , Carcinome lobulaire/anatomopathologie , Diagnostic différentiel , Femelle , Humains , Kératines/immunologie , Kératines/métabolisme , Sensibilité et spécificité , Facteurs de transcription/immunologie , Facteurs de transcription/métabolisme , bêta-Caténine/immunologie , bêta-Caténine/métabolisme
15.
Arq Bras Oftalmol ; 75(1): 64-6, 2012.
Article de Anglais | MEDLINE | ID: mdl-22552422

RÉSUMÉ

A 78-year-old male patient presented with double vision, painless palpable mass under the right superolateral orbital rim, downward displacement and restricted adduction of the right eye. His visual acuity was 20/50 OD and 20/20 OS. Hertel exophthalmometry was 21 mm OD and 17 mm OS. Computed tomographic scans showed an infiltrative orbital mass with ill-defined, irregular margins, involving the lacrimal gland and the lateral rectus muscle. The patient underwent an anterior transcutaneous transseptal orbitotomy with incisional biopsy and surgical debulking. Histopathologic evaluation revealed primary ductal adenocarcinoma of the lacrimal gland. Following the metastatic work up, he underwent an eyelid-sparing orbital exenteration. Microscopically, the tumor elements were characterized by large polygonal cells with vesicular nuclei, prominent nucleoli and amphophilic cytoplasm. The tumor components comprised duct-type structures with papillary and cribriform patterns, surrounded by prominent basement membrane. The tumor cells were positive for cytokeratin-7, matrix metalloproteinase (MMP)-2, MMP-9, MMP-13 and proto-oncogene Her-2/neu, but negative for cytokeratin-5, cytokeratin-20, p63, prostate-specific antigen, S-100 protein and thyroid transcription factor. These histopathologic findings were compatible with poorly differentiated ductal adenocarcinoma of the lacrimal gland, T3N0M0. Twenty-four months after orbital exenteration, the patient was diagnosed with ipsilateral parotid gland and cervical lymph node metastases and died of disease.


Sujet(s)
Carcinome canalaire/anatomopathologie , Tumeurs de l'oeil/anatomopathologie , Maladies de l'appareil lacrymal/anatomopathologie , Appareil lacrymal/anatomopathologie , Tumeurs de la parotide/secondaire , Sujet âgé , Biopsie , Carcinome canalaire/chirurgie , Tumeurs de l'oeil/chirurgie , Issue fatale , Humains , Appareil lacrymal/chirurgie , Maladies de l'appareil lacrymal/chirurgie , Métastase lymphatique , Mâle , Proto-oncogène Mas , Tomodensitométrie
16.
Eur J Med Res ; 17: 8, 2012 Apr 25.
Article de Anglais | MEDLINE | ID: mdl-22534285

RÉSUMÉ

This report describes a rare case of coexistence of benign phyllodes tumor, which measured 9 cm in the right breast, and invasive ductal carcinoma of 6 cm in the left breast, synchronous and independent, in a 66-year-old patient. The patient underwent a bilateral mastectomy due to the size of both lesions. Such situations are rare and usually refer to the occurrence of ductal or lobular carcinoma in situ when associated with malignant phyllodes tumors, and more often in ipsilateral breast or intra-lesional.


Sujet(s)
Tumeurs du sein , Carcinome canalaire , Invasion tumorale/anatomopathologie , Tumeur phyllode , Tumeurs du sein/complications , Tumeurs du sein/anatomopathologie , Tumeurs du sein/chirurgie , Carcinome canalaire/complications , Carcinome canalaire/anatomopathologie , Carcinome canalaire/chirurgie , Femelle , Humains , Tumeur phyllode/complications , Tumeur phyllode/anatomopathologie , Tumeur phyllode/chirurgie
17.
Rev. venez. oncol ; 24(1): 57-60, ene.-mar. 2012. ilus
Article de Espagnol | LILACS | ID: lil-704404

RÉSUMÉ

El carcinoma papilar intraquístico es un subtipo de carcinoma ductal no invasivo, puede estar asociado o no a carcinoma in situ y a carcinoma infiltrante, es unapatología poco frecuente por tanto su aparición en hombres es extremadamente rara. El tratamiento en estos casos es equivalente al carcinoma de mama en mujeres, debatiéndose entre mastectomía simple o mastectomía parcial más radioterapia. Presentamos el caso de un hombre a quien se diagnostica preoperatoriamente con un carcinoma papilar y se realiza mastectomía simple más ganglio centinela


The papillary intra cyst carcinoma is a sub type of ductal no invasive carcinoma, it can be associated or no to carcinoma in situ and infiltrate carcinoma, is less frequent, for this reason his apparition in male is rare. The treatment in is equivalent to breast carcinoma in women, with two modalities of treatment: simple mastectomy or simple mastectomy with radiation therapy. We present clinical case of male whom is diagnostic pre surgery with a papillary carcinoma and realized simple mastectomy with sentinel node


Sujet(s)
Humains , Mâle , Biopsie de noeud lymphatique sentinelle/méthodes , Carcinome canalaire/anatomopathologie , Carcinome papillaire/anatomopathologie , Mastectomie simple/méthodes , Tumeur du sein de l'homme/chirurgie , Tumeur du sein de l'homme/diagnostic , Tumeur du sein de l'homme/anatomopathologie , Radiothérapie/méthodes , Oncologie médicale
18.
Arq. bras. oftalmol ; Arq. bras. oftalmol;75(1): 64-66, jan.-fev. 2012.
Article de Anglais | LILACS | ID: lil-622550

RÉSUMÉ

A 78-year-old male patient presented with double vision, painless palpable mass under the right superolateral orbital rim, downward displacement and restricted adduction of the right eye. His visual acuity was 20/50 OD and 20/20 OS. Hertel exophthalmometry was 21 mm OD and 17 mm OS. Computed tomographic scans showed an infiltrative orbital mass with ill-defined, irregular margins, involving the lacrimal gland and the lateral rectus muscle. The patient underwent an anterior transcutaneous transseptal orbitotomy with incisional biopsy and surgical debulking. Histopathologic evaluation revealed primary ductal adenocarcinoma of the lacrimal gland. Following the metastatic work up, he underwent an eyelid-sparing orbital exenteration. Microscopically, the tumor elements were characterized by large polygonal cells with vesicular nuclei, prominent nucleoli and amphophilic cytoplasm. The tumor components comprised duct-type structures with papillary and cribriform patterns, surrounded by prominent basement membrane. The tumor cells were positive for cytokeratin-7, matrix metalloproteinase (MMP)-2, MMP-9, MMP-13 and proto-oncogene Her-2/neu, but negative for cytokeratin-5, cytokeratin-20, p63, prostate-specific antigen, S-100 protein and thyroid transcription factor. These histopathologic findings were compatible with poorly differentiated ductal adenocarcinoma of the lacrimal gland, T3N0M0. Twenty-four months after orbital exenteration, the patient was diagnosed with ipsilateral parotid gland and cervical lymph node metastases and died of disease.


Paciente do sexo masculino e com 78 anos de idade apresentou diplopia, massa palpável abaixo da margem orbitária direita, deslocamento inferior do bulbo ocular direito e limitação da adução do olho direito. A acuidade visual foi 20/50 OD e 20/20 OE. A exoftalmometria de Hertel foi 21 mm OD e 17 mm OE. Tomografia computadorizada mostrou uma massa orbitária, infiltrativa e com margens irregulares, envolvendo a glândula lacrimal e o músculo reto lateral. O paciente foi submetido a uma orbitotomia anterior com biópsia incisional. O exame histopatológico revelou adenocarcinoma ductal primário da glândula lacrimal. Em seguida, o paciente foi submetido a uma exenteração orbitária com preservação das pálpebras. Microscopicamente, os elementos tumorais foram caracterizados por células poligonais grandes com citoplasma anfofílico, núcleo vesicular e nucléolo proeminente. Os componentes tumorais incluíram estruturas ductais com padrões cribriforme e papilífero e cercadas por membrana basal proeminente. As células tumorais foram positivas para citoqueratina 7, metaloproteinase 2 da matriz, metaloproteinase 9 da matriz, metaloproteinase 13 da matriz e Her-2/neu, mas negativas para citoqueratina 5, citoqueratina 20, p63, antígeno prostático específico, proteína S-100 e fator de transcrição da tiroide. Estes achados histopatológicos foram compatíveis com o diagnóstico de adenocarcinoma ductal pouco diferenciado da glândula lacrimal, T3N0M0. Vinte e quatro meses após a exenteração orbitária, o paciente foi diagnosticado com metástases nos linfonodos cervicais ipsilaterais e na glândula parótida ipsilateral e faleceu.


Sujet(s)
Sujet âgé , Humains , Mâle , Carcinome canalaire/anatomopathologie , Tumeurs de l'oeil/anatomopathologie , Maladies de l'appareil lacrymal/anatomopathologie , Appareil lacrymal/anatomopathologie , Tumeurs de la parotide/secondaire , Biopsie , Carcinome canalaire/chirurgie , Tumeurs de l'oeil/chirurgie , Issue fatale , Métastase lymphatique , Maladies de l'appareil lacrymal/chirurgie , Appareil lacrymal/chirurgie , Tomodensitométrie
19.
Acta Histochem ; 114(3): 226-31, 2012 May.
Article de Anglais | MEDLINE | ID: mdl-21683430

RÉSUMÉ

The aim of the study was to assess the relationship between the expression of COX-2 and p53, hormone receptors and HER-2 in the in situ (DCIS) and invasive components of ductal carcinomas (IDC) of the same breast. The expression of COX-2, p53, and hormone receptors was assessed in 87 cases of IDC with contiguous areas of DCIS. Results showed that there was no difference in COX-2 expression comparing the in situ and invasive components of the tumors. In the in situ component, there was a statistically borderline increase in p53 expression in tumors that also expressed COX-2. ER-positive specimens were more common in the group of tumors that expressed COX-2 in the invasive component. From this study we conclude that the expression of COX-2 was similar in the in situ and invasive components of the breast carcinomas. COX-2 positivity was marginally related with the expression of p53 in the in situ components, and with the ER expression in the invasive components.


Sujet(s)
Tumeurs du sein/anatomopathologie , Région mammaire/anatomopathologie , Carcinome canalaire/anatomopathologie , Cyclooxygenase 2/métabolisme , Protéine p53 suppresseur de tumeur/métabolisme , Sujet âgé , Marqueurs biologiques tumoraux/génétique , Marqueurs biologiques tumoraux/métabolisme , Région mammaire/métabolisme , Tumeurs du sein/génétique , Tumeurs du sein/métabolisme , Carcinome canalaire/génétique , Carcinome canalaire/métabolisme , Cyclooxygenase 2/génétique , Femelle , Expression des gènes , Humains , Immunohistochimie , Hybridation fluorescente in situ , Adulte d'âge moyen , Invasion tumorale , Récepteur ErbB-2/génétique , Récepteur ErbB-2/métabolisme , Récepteurs des oestrogènes/génétique , Récepteurs des oestrogènes/métabolisme , Microenvironnement tumoral , Protéine p53 suppresseur de tumeur/génétique
20.
Genet Mol Res ; 10(4): 2430-42, 2011 Oct 03.
Article de Anglais | MEDLINE | ID: mdl-21968807

RÉSUMÉ

Breast cancer is a complex and heterogeneous disease. In spite of the advances made in recent decades, a better understanding of the intrinsic mechanisms of this disease is crucial. The development of new biomarkers is absolutely necessary to improve diagnosis and prognosis. Research using the proteomic approach has generated interesting results; however, the complexity of the mammary gland and of breast tumors remains a major limitation to the development of new markers. An initial step is to characterize non-tumoral human breast tissue. We present data from classical proteomic analysis based on 2-D electrophoresis and peptide mass fingerprinting identification, which were performed on six non-tumoral samples from patients with invasive ductal breast carcinomas. Forty-four different proteins from 70 spots were identified and classified according to their biological function. Cytoskeleton and associated proteins represent the largest class (30%) followed by the proteins with binding function (27%). Several of the proteins have been described in breast tumors, such as vimentin, endoplasmin, small heat shock beta-6, disulfide isomerase and some cell growth, and proliferation regulators, suggesting the importance of including data on the characterization of non-tumoral breast and to studies on differential expression in cancer tissue.


Sujet(s)
Glandes mammaires humaines/métabolisme , Protéome/métabolisme , Protéomique , Sujet âgé , Marqueurs biologiques tumoraux/métabolisme , Tumeurs du sein/diagnostic , Tumeurs du sein/métabolisme , Tumeurs du sein/anatomopathologie , Carcinome canalaire/diagnostic , Carcinome canalaire/métabolisme , Carcinome canalaire/anatomopathologie , Femelle , Humains , Glandes mammaires humaines/anatomopathologie , Adulte d'âge moyen , Pronostic
SÉLECTION CITATIONS
DÉTAIL DE RECHERCHE