RÉSUMÉ
Bone morphogenetic proteins (BMPs) play an important role during the initial process of enamel development and therefore may play a role in caries susceptibility. The purpose of this study was to evaluate the association between the polymorphisms in the BMP2, BMP4 and BMP7 genes and their association with caries experience and primary enamel microhardness characteristics. DNA from buccal cells as well as clinical and demographic information from 1,731 subjects from three different data sets from Brazil were included. Polymorphisms in BMP2, BMP4 and BMP7 were analyzed by real-time polymerase chain reaction from genomic DNA. Association between caries experience, genotype, and allele distribution in both cohorts was evaluated using χ(2) and logistic regression analyses. In the family-based set, the association between caries experience and alleles was tested using the transmission disequilibrium test. In the Rio de Janeiro cohort, microhardness data on 108 exfoliated primary teeth before and after demineralization and remineralization challenges was included. Associations between microhardness values and genotype and allele distribution were evaluated using χ(2) and logistic regression analyses. Differences between caries experience and some risk factors were statistically significant. In the cohort from Nova Friburgo, BMP2 was associated with caries experience in primary dentition during logistic regression analysis (p = 0.023; OR = 2.58; 95% CI 1.13-5.86). There was no association between genotype and allele distribution for BMP polymorphisms and primary enamel microhardness alterations. Our result suggests that BMP2 may be involved in caries experience in primary dentition from a Nova Friburgo cohort.
Sujet(s)
Protéine morphogénétique osseuse de type 2/génétique , Indice DCAO , Caries dentaires/enzymologie , Polymorphisme génétique/génétique , Dent de lait/enzymologie , Adolescent , Protéine morphogénétique osseuse de type 4/génétique , Protéine morphogénétique osseuse de type 7/génétique , Brésil , Enfant , Enfant d'âge préscolaire , Études de cohortes , Caries dentaires/génétique , Dispositifs d'hygiène buccodentaire à usage domestique/statistiques et données numériques , Émail dentaire/anatomie et histologie , Comportement alimentaire , Femelle , Fréquence d'allèle/génétique , Variation génétique/génétique , Génotype , Dureté , Humains , Nourrisson , Déséquilibre de liaison/génétique , Mâle , Polymorphisme de nucléotide simple/génétique , Reminéralisation des dents , Brossage dentaire/statistiques et données numériques , Jeune adulteRÉSUMÉ
Nucleotide content and activity of certain enzymes were compared in pigs of various ages in order to study the energetic metabolism of deciduous dental pulps in the three phases of the cycle of tooth ontogeny, namely, root formation, fully formed root and root resorption phases. The frozen pulps were removed with the help of a screw vise and analysed for ATP, ADP and AMP contents and Ca2+ and Mg2+-ATPases activities. The highest ATP content in the first deciduous molar pulp was found when the tooth was still in an intrabony position. The calculated energy charge, although low for all groups, at this stage of development, indicated an activation of the consuming processes. In the root resorption phase, lowest ATP content and higher Ca2+ and Mg2+-ATPases activities were observed.