Long-term adaptation of water hyacinth to low cadmium involves antioxidant enzyme and metallothionein transcriptional regulation.

Cadmium (Cd) is one of the main heavy metal pollutants in environment. Water hyacinth (Eichhornia crassipes) is an effective phytoremediation plant for mitigating Cd stress, though the concentration threshold for its long-term survival remains unclear. Our results indicate that 4 mg L-1 Cd may be the maximum threshold for long-term cultivation of water hyacinth, as it significantly inhibits root growth and photosynthesis. The activity of superoxide dismutase increased under low concentration Cd treatment (0.5 and 1.0 mg L-1), potentially contributing to the reactive oxygen species (ROS) homeostasis in water hyacinth. Additionally, we identified the Cd-induced metallothionein gene MT1, whose heterologous expression in yeast enhanced Cd tolerance despite higher Cd accumulation. The upregulation of MT1 may enhance the detoxification capacity of water hyacinth under Cd stress. Our findings establish the Cd range for long-term cultivation of water hyacinth and elucidated the mechanism of its tolerance to moderate Cd stress.

Environmental concentrations of 6PPD and 6PPD-Q cause oxidative damage and alter metabolism in Eichhornia crassipes.

N-(1,3-dimethylbutyl)-N '-phenyl-p-phenylenediamine (6PPD) and N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine-quinone (6PPD-Q) are ubiquitous in the environment and can cause toxicity to aquatic animals. However, research on the toxicological effects of 6PPD and 6PPD-Q on aquatic plants remains limited. The present study investigated the physiological, biochemical, and metabolic responses of the floating aquatic plant Eichhornia crassipes (E. crassipes) to environmentally relevant concentrations (0.1, 1, and 10 µg·L-1) of 6PPD and 6PPD-Q. We found that 6PPD and 6PPD-Q elicited minimal effects on plant growth, but 6PPD induced a concentration-dependent decrease in the content of photosynthetic pigments. Low doses (0.1 µg·L-1 and 1 µg·L-1) of 6PPD-Q significantly elevated Reactive Oxygen Species (ROS) content in E. crassipes roots, indicating oxidative damage. Furthermore, 6PPD-Q induced a more pronounced osmotic stress compared to 6PPD. Metabolic analyses revealed that carbohydrates were significantly altered under 6PPD and 6PPD-Q treatments. The findings of this study enhance the understanding of the environmental risks posed by 6PPD and 6PPD-Q to plants and reveal the potential mechanisms of phytotoxicity.

Mitigation of ammonia volatilization from organic and inorganic nitrogen sources applied to soil using water hyacinth biochars.

The recent global population explosion has increased people's food demand. To meet this demand, huge amounts of nitrogen (N) fertilizer have been applied in the worldwide. However, ammonia (NH3) volatilization is one of the primary factors of N loss from soil after N application causing decrease crop N utilization efficiency and productivity. Incubation experiments were conducted on an acidic clayey soil with two different N sources (urea and anaerobic digestion effluent; ADE), two differently-produced biochars, and three biochar application rates (0%, 0.25%, and 1.0% w/w). Ammonia volatilization was lower from urea (14.0-23.5 mg N kg-1) and ADE (11.3-21.0 mg N kg-1) with biochar application than those without biochar (40.1 and 26.2 mg N kg-1 from urea and ADE alone, respectively). Biochar application significantly mitigated volatilization and reduction percentages for urea and ADE were 40%-64% and 18%-55%, respectively. 1.0% biochar application mitigated volatilization significantly compared to 0.25% application regardless of N source and biochar types. Possible mechanism for volatilization mitigation for urea and ADE were increased N immobilization by soil microorganisms and accelerated net nitrification rate due to increased soil nitrifying bacteria, respectively. Overall, our results clarified different mechanisms for N volatilization mitigation from different (inorganic vs. organic) N sources with biochar application.

Unlocking the potential of Eichhornia crassipes for wastewater treatment: phytoremediation of aquatic pollutants, a strategy for advancing Sustainable Development Goal-06 clean water.

The 2030 Agenda, established in 2015, contains seventeen Sustainable Development Goals (SDGs) aimed at addressing global challenges. SDG-06, focused on clean water, drives the increase in basic sanitation coverage, the management of wastewater discharges, and water quality. Wastewater treatment could contribute to achieving 11 of the 17 SDGs. For this purpose, phytoremediation is a low-cost and adaptable alternative to the reduction and control of aquatic pollutants. The objective of this study is to highlight the role of macrophytes in the removal and degradation of these compounds, focusing on Eichhornia crassipes (Mart.) Solms, commonly known as water hyacinth. The reported values indicate that this plant has a removal capacity of over 70% for metals such as copper, aluminum, lead, mercury, cadmium, and metalloids such as arsenic. Additionally, it significantly improves water quality parameters such as turbidity, suspended solids, pH, dissolved oxygen, and color. It also reduces the presence of phosphates, and nitrogen compounds to values below 50%. It also plays a significant role in the removal of organic contaminants such as pesticides, pharmaceuticals, and dyes. This study describes several valuable by-products from the biomass of the water hyacinth, including animal and fish feed, energy generation (such as briquettes), ethanol, biogas, and composting. According to the analysis carried out, E. crassipes has a great capacity for phytoremediation, which makes it a viable solution for wastewater management, with great potential for water ecosystem restoration.

Effects of long-chained perfluoroalkyl acids (PFAAs) on the uptake and bioaccumulation of short-chained PFAAs in two free-floating macrophytes: Eichhornia crassipes and Ceratophyllum demersum.

Short-chained perfluoroalkyl acids (PFAAs, CnF2n+1-R, n ≤ 6) have merged as global concerns due to their extensive application and considerable toxicity. However, long-chained PFAAs (n ≥ 7) featured with high persistence are still ubiquitously observed in aquatic environment. To understand the uptake behavior of short-chained PFAAs in aquatic macrophytes, the uptake kinetics, bioconcentration, and translocation of short-chained PFAAs (3 ≤n ≤ 6) in two typical free-floating macrophytes (Eichhornia crassipes and Ceratophyllum demersum) were investigated in the treatments with and without long-chained PFAAs (7 ≤n ≤ 11). Results showed that short-chained PFAAs can be readily accumulated in both E. crassipes and C. demersum, and the uptake of short-chained PFAAs fit the two-compartment kinetic model well (p < 0.05). In the treatments with long-chained PFAAs, significant concentration decreases of all concerned short-chained PFAAs in E. crassipes and PFAAs with n ≤ 5 in C. demersum were observed. Long-chained PFAAs could hinder the uptake rates, bioconcentration factors, and translocation factors of most short-chained PFAAs in free-floating macrophytes (p < 0.01). Significant correlations between bioconcentration factors and perfluoroalkyl chain length were only observed when long-chained PFAAs were considered (p < 0.01). Our results underlined that the effects of long-chained PFAAs should be taken into consideration in understanding the uptake and bioaccumulation behaviors of short-chained PFAAs.

The bioaccumulation and ecotoxicity of co-exposure of per(poly)fluoroalkyl substances and polystyrene microplastics to Eichhornia crassipes.

Gen X and F-53B have been popularized as alternatives to PFOA and PFOS, respectively. These per(poly)fluoroalkyl substances pervasively coexist with microplastics (MPs) in aquatic environments. However, there are knowledge gaps regarding their potential eco-environmental risks. In this study, a typical free-floating macrophyte, Eichhornia crassipes (E. crassipes), was selected for hydroponic simulation of a single exposure to PFOA, PFOS, Gen X, and F-53B, and co-exposure with polystyrene (PS) microspheres. F-53B exhibited the highest bioaccumulation followed by Gen X, PFOA, and PFOS. In the presence of PS MPs, the bioavailabilities of the four PFASs shifted and the whole plant bioconcentration factors improved. All four PFASs induced severe lipid peroxidation, which was exacerbated by PS MPs. The highest integrated biomarker response (IBR) was observed for E. crassipes (IBR of shoot: 30.01, IBR of root: 22.79, and IBR of whole plant: 34.96) co-exposed to PS MPs and F-53B. The effect addition index (EAI) model revealed that PS MPs showed antagonistic toxicity with PFOA and PFOS (EAI < 0) and synergistic toxicity with Gen X and F-53B (EAI > 0). These results are helpful to compare the eco-environmental impacts of legacy and alternative PFASs for renewal process of PFAS consumption and provide toxicological, botanical, and ecoengineering insights under co-contamination with MPs.

Valorization of Eichhornia crassipes for the production of cellulose nanocrystals further investigation of plethoric biobased resource.

Chemical processing is among the significant keys to tackle agro-residues utilization field, aiming to obtain value-added materials. Extraction of cellulose nanocrystals (CNCs) is an emerging route to valorize lignocellulosic wastes into high value particles. In this investigation, effect of acidic hydrolysis duration was monitored on size and morphology of obtained crystals; namely: CNCs from Nile roses fibers (NRFs) (Eichhornia crassipes). Different acidic hydrolysis duration range or different characterization techniques set this article apart from relevant literature, including our group research articles. The grinded NRFs were firstly subjected to alkaline and bleaching pretreatments, then acid hydrolysis process was carried out with varied durations ranging from 5 to 30 min. Microcrystalline cellulose (MCC) was used as reference for comparison with NRFs based samples. The extracted CNCs samples were investigated using various techniques such as scanning electron microscopy (SEM), Atomic force microscopy (AFM), Raman spectroscopy, and thermogravimetric (TGA) analysis. The figures gotten from SEM and AFM depicted that NRFs based CNCs appeared as fibril-like shapes, with reduced average size when the NRFs underwent pulping and bleaching processes. This was indicated that the elimination of hemicellulose and lignin components got achieved successfully. This outcome was proven by chemical composition measurements and TGA/DTG curves. On the other hand, AFM-3D images indicated that CNCs topology and surface roughness were mostly affected by increasing hydrolysis durations, besides smooth and homogeneous surfaces were noticed. Moreover, Raman spectra demonstrated that the particle size and crystallinity degree of NRFs based CNCs can be affected by acidic hydrolysis durations and optimum extraction time was found to be 10 min. Thermal stability of extracted CNCs-NRFs and CNCs-MCC was measured by TGA/DTG and the kinetic models were suggested to identify the kinetic parameters of the thermal decomposition of CNCs for each acid hydrolysis duration. Increasing hydrolysis duration promoted thermal stability, particularly for NRFs based CNCs. Results showcased in this article add new perspective to Nile rose nanocellulose and pave down the way to fabricate NRFs based humidity nano-sensors.

Alien emergent aquatic plants develop better ciprofloxacin tolerance and metabolic capacity than one native submerged species.

Antibiotic pollution and biological invasion pose significant risks to freshwater biodiversity and ecosystem health. However, few studies have compared the ecological adaptability and ciprofloxacin (CIPR) degradation potential between alien and native macrophytes. We examined growth, physiological response, and CIPR accumulation, translocation and metabolic abilities of two alien plants (Eichhornia crassipes and Myriophyllum aquaticum) and one native submerged species (Vallisneria natans) exposed to CIPR at 0, 1 and 10 mg/L. We found that E. crassipes and M. aquaticum's growth were unaffected by CIPR while V. natans was significantly hindered under the 10 mg/L treatment. CIPR significantly decreased the maximal quantum yield of PSII, actual quantum yield of PSII and relative electron transfer rate in E. crassipes and V. natans but didn't impact these photosynthetic characteristics in M. aquaticum. All the plants can accumulate, translocate and metabolize CIPR. M. aquaticum and E. crassipes in the 10 mg/L treatment group showed greater CIPR accumulation potential than V. natans indicated by higher CIPR contents in their roots. The oxidative cleavage of the piperazine ring acts as a key pathway for these aquatic plants to metabolize CIPR and the metabolites mainly distributed in plant roots. M. aquaticum and E. crassipes showed a higher production of CIPR metabolites compared to V. natans, with M. aquaticum exhibiting the strongest CIPR metabolic ability, as indicated by the most extensive structural breakdown of CIPR and the largest number of potential metabolic pathways. Taken together, alien species outperformed the native species in ecological adaptability, CIPR accumulation and metabolic capacity. These findings may shed light on the successful invasion mechanisms of alien aquatic species under antibiotic pressure and highlight the potential ecological impacts of alien species, particularly M. aquaticum. Additionally, the interaction of antibiotic contamination and invasion might further challenge the native submerged macrophytes and pose greater risks to freshwater ecosystems.

Design of a sustainable system for wastewater treatment and generation of biofuels based on the biomass of the aquatic plant Eichhornia Crassipes.

Colombia's continuous contamination of water resources and the low alternatives to produce biofuels have affected the fulfillment of the objectives of sustainable development, deteriorating the environment and affecting the economic productivity of this country. Due to this reality, projects on environmental and economic sustainability, phytoremediation, and the production of biofuels such as ethanol and hydrogen were combined. The objective of this article was to design and develop a sustainable system for wastewater treatment and the generation of biofuels based on the biomass of the aquatic plant Eichhornia crassipes. A system that simulates an artificial wetland with live E. crassipes plants was designed and developed, removing organic matter contaminants; subsequently, and continuing the sustainability project, bioreactors were designed, adapted, and started up to produce bioethanol and biohydrogen with the hydrolyzed biomass used in the phytoremediation process, generating around 12 g/L of bioethanol and around 81 ml H2/g. The proposed research strategy suggests combining two sustainable methods, bioremediation and biofuel production, to preserve the natural beauty of water systems and their surroundings.

Uptake of perfluoroalkyl substances PFOS and PFOA by free-floating hydrophytes Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms.

The phytoremediation potential of floating aquatic plants to accumulate and remove two common PFAS from contaminated water was investigated. Free-floating hydrophytes Eichhornia crassipes and Pistia stratiotes were grown in water spiked with 0.5, 1, or 2 ppm perfluorooctanoic acid (PFOA) or perfluorooctanesulfonic acid (PFOS) for seven days. Both species were able to accumulate PFOA and PFOS in this time frame, with translocation factors (TF) ranging from 0.13 to 0.57 for P. stratiotes and 0.18 to 0.45 for E. stratiotes, respectively. E. crassipes accumulated a greater amount of PFOA and PFOS than P. stratiotes, with 178.9 ug PFOA and 308.5 ug PFOS removed by E. crassipes and 98.9 ug PFOA and 137.8 ug PFOS removed by P. stratiotes at the highest concentrations. Root tissue contained a higher concentration of PFOA and PFOS than shoot tissue in both species, and the concentration of PFOS was generally significantly higher than PFOA in both E. crassipes and P. stratiotes, with concentrations of 15.39 and 27.32 ppb PFOA and 17.41 and 80.62 ppb PFOS in shoots and roots of P. stratiotes and 12.59 and 37.37 ppb PFOA and 39.92 and 83.40 ppb PFOS in shoots and roots of E. crassipes, respectively. Both species may be candidates for further phytoremediation studies in aquatic ecosystems.

This study investigates the feasibility of using wetland plants for the phytoremediation of PFAS. Prior published studies examine various plant interactions with PFAS but do not evaluate remediation potential of P. stratiotes.

Insights of phytoremediation mechanisms for viruses based on in-vitro, in-vivo and in-silico assessments of selected herbal plants.

Waterborne pathogenic viruses present unrelenting challenges to the global health and wastewater treatment industry. Phytoremediation offers promising solutions for wastewater treatment through plant-based technologies. This study investigated antiviral mechanisms in-vivo using bacteriophages MS2 and T4 as surrogates for effective herbs screened in-vitro from three embryophytes (Ocimum basilicum, Mentha sp., Plectranthus amboinicus), two macrophytes (Eichhornia crassipes, Pistia stratiotes) and a perennial grass (Cyperus rotundas). In-silico virtual screening predicted antiviral phytochemicals for further antiviral potency assessment. Results suggested in-vitro antiviral activities of embryophytes and macrophytes were higher (43-62%) than grass (21-26%). O. basilicum (OB, 57-62%) and P. stratiotes (PS, 59-60%) exhibited the highest antiviral activities. In-vivo tests showed notable virus reduction (>60%) in culture solution, attributed to rhizofiltration (66-74%) and phytoinactivation/phytodegradation (63-84%). In-silico analysis identified rutin as a primary antiviral phytochemical for MS2 (-9.7 kcal/mol) and T4 (-10.9 kcal/mol), correlating with dose-response inactivation (∼58-62%). In-vivo tests suggested additional phytocompounds may contribute to viral inactivation, presenting new opportunities for herb-based wastewater treatment solutions. Consequently, this study not only demonstrates the antiviral capabilities of OB and PS but also introduces an innovative approach for addressing viral contaminants in water.

Dual phytoremediation and biochar production by Eichhornia crassipes in hydroponic system receiving different 1,4-dioxane dosages.

This study focuses on applying phytoremediation as a low-effective and simple process to treat wastewater laden with 1,4 dioxane (DIOX). A floating macrophyte (Eichhornia crassipes) was cultivated under hydroponic conditions (relative humidity 50-67%, photoperiod cycle 18:6 h light/dark, and 28-33 °C) and subjected to different DIOX loads between 0.0 (control) and 11.5 mg/g fresh mass (FM). The aquatic plant achieved DIOX and chemical oxygen demand (COD) removal efficiencies of 76-96% and 67-94%, respectively, within 15 days. E. crassipes could tolerate elevated DIOX-associated stresses until a dose of 8.2 mg DIOX/g, which highly influenced the oxidative defense system. Malondialdehyde (MDA) content, hydrogen peroxide (H2O2), and total phenolic compounds (TPC) increased by 7.3, 8.4, and 4.5-times, respectively, in response to operating the phytoremediation unit at a DIOX load of 11.5 mg/g. The associated succulent value, proteins, chlorophyll-a, chlorophyll-b, and pigments dropped by 39.6%, 45.8%, 51.5%, 80.8%, and 55.5%, respectively. The suggested removal mechanism of DIOX by E. crassipes could be uptake followed by phytovolatilization, whereas direct photodegradation from sunlight contributed to about 19.36% of the total DIOX removal efficiencies. Recycling the exhausted E. crassipes for biochar production was a cost-efficient strategy, making the payback period of the phytoremediation project equals to 6.96 yr.

Eichhornia crassipes could be used in phytoremediation of 1,4 dioxane (DIOX)-laden water at DIOX load< 8.2 mg/g FM. E. crassipes removed 77­97% DIOX via uptake and phytovolatilization. Recycling exhausted-plant to produce biochar was cost-efficient with 7 yr-payback period.

Evaluation of water hyacinth utility through geospatial mapping and in situ biomass estimation approach: a case study of Deepor beel (wetland), Assam, India.

As an invasive species, water hyacinths (Eichhornia crassipes) are known to progressively proliferate and cause the ecological invasions of the aquatic environment. The incursions of the water hyacinths not only cause the disappearance of native species but gradually degrade the natural habitats of freshwater regimes. The control and management of these species are laborious task; however, transforming weed into wealth can substantially serve a sustainable approach to reduce the efforts. Therefore, the present study intends to utilize the application of geospatial techniques for mapping the water hyacinths growth in the Deepor beel (wetland) of Assam, India. Sentinel based image analysis has shown that pre-monsoon seasons has encountered massive productivity and area coverage of water hyacinth, whereas in post-monsoon seasons, productivity of water hyacinths reduces to half. Furthermore, in situ biomass estimation of the water hyacinth samples, same around the productive season has been collected and was analyzed as 6 kg (green biomass) and 1 kg (dry biomass after sun-dried). Finally, this hybrid approach evaluated the production and revenue generation from Moorhen yoga mat (handicraft item) made from the dried water hyacinths. After assuming the actual availability of 50% of total mass yield of water hyacinths, around ~ 0.8 million (8.8 lakhs) yoga mats can be commercially produced within the most productive seasons. The revenue generation from the yoga mat in the domestic and international markets evaluated around US $12.79 million (Rs. 105.85 crore) and US $15.99 million (Rs. 132.31 crore), respectively, from a single productive season. Thus, applicative intent of this study can boost potential market in Assam, renovate the weed waste of water hyacinth into wealth generation, and sustainably support the livelihoods of the local communities.

Mathematical modeling of dark fermentative hydrogen and soluble by-products generations from water hyacinth.

The production of hydrogen and soluble metabolite products from water hyacinth via dark fermentation was modeled. The model was built on the assumption that the substrate exists in two forms (i.e., soluble and particulate) and undergoes two stages (i.e., hydrolysis and acidogenesis) in the dark fermentation process. The modified Michaelis-Menten and surface-limiting models were applied to describe the hydrolysis of soluble and particulate forms, respectively. Meanwhile, the acidogenesis stage was modeled based on the multi-substrate-single-biomass model. The effects of temperature, pH, and substrate concentration were integrated into the model to increase flexibility. As a result, the model prediction agreed with the experimental and literature data of water hyacinth-fed dark fermentation, with high coefficient of determination values of 0.92 - 0.97 for hydrogen and total soluble metabolite products. These results indicate that the proposed model could be further applied to dark fermentation's downstream and hybrid processes using water hyacinth and other substrates.

Bioconversion of Eichhornia crassipes into vermicompost on a large scale through improving operational aspects of in-vessel biodegradation process: Microbial dynamics.

Eichhornia crassipes is a common, abundant aquatic weed biomass found globally. The present study examined optimum biodegradation procedures through batch studies (550 L rotating drum composter) and the resulting best combination on a large scale (5000 L rotary drum composter). The pilot scale rotary drum reactor was commenced with cow manure and then treated for 3 months with 250 kg/day of homogenously mixed E. crassipes and dry leaves. The rotary drum's inlet and outlet temperatures were 60 °C and 39 °C, respectively, suggesting thermophilic conditions with a 7-day waste retention duration. Eisenia fetida was used for vermicomposting the outlet material for 20 days, raising the nitrogen content to 3.2%. Bacterial diversity (16S-rRNA) sequencing revealed that Proteobacteria and Euryarchaeota are the most predominant. After 27 days, the volume dropped by 71%, and the product was stable and soil-safe. Large-scale optimised biodegradation may be a better way to handle aquatic weed biomass.

Comparison of pennywort and hyacinth in the development of membraned sediment plant microbial fuel cell for waste treatment.

Membraned Sediment Plant Microbial Fuel cells (SPMFCs) are appealing bioelectrochemical systems that generate power from organic compounds in sediment through exoelectrogen decomposition and are used to treat wastewater. This research was designed to develop a single-chambered sediment plant microbial fuel cell using two membrane electrodes; one carbon plate cathode and one anode. Wastewater and sediment mixture was sampled from Rawalpindi, Pakistan, and bacterial isolation was performed by serial dilution. Five strains were selected on the basis of morphology and growth-promoting characteristics. The selected strains were identified by 16s rRNA sequencing and designated as A (Geobacter sulfurreducens OP527025), B (Shawanella putrefaciens OP522353), C (Bacillus subtilus OP522349), D (Azospirillum humicireducens OP527050) and E (Pseudomonas putida OP526951). Consortium of five strains was developed. Two aquatic plants pennyworts (Hydrocotyle umbellate), and Hyacinth (Eichhornia crassipes) were used in the SPMFCs along with consortium. A maximum voltage of 1120mv was observed in SPMFCs treated with the consortium and water hyacinth, which was followed by 543.3 mv of SPMFCs treated with water pennyworts. Physicochemical analysis of wastewater showed a remarkable reduction of 74.5%, 71%, and 76% in nitrate, phosphate, and sulphate content of wastewater treated with microbes and water hyacinth. The heavy metal analysis showed a reduction of Zn (99.8%), Mg (99.9%), and Ni (98.4%) in SPMFCs treated with the consortium and water hyacinth. Mebraned SPMFCs showed an increase of 30% and 20% in shoot and root length of water hyacinth. A remarkable increase of 25%, 18%, and 12% were recorded in chlorophyll content, membrane stability index and relative water content of water hyacinth in SPMFCs treated with consortium compared to untreated cells. Osmolyte content had shown significant increase of 25% with consortium treated water hyacinth plant as compared to untreated one. An increase of 15%, 20% and 12% was noted in superoxide dismutase (SOD), peroxidase dismutase (POD) and catalase content of consortium treated water hyacinth as compared to control one. The present research gave insight into the potential of sediment plant microbial fuel cells along with aquatic plants for treatment of wastewater. This could be a effective method for removal of hazrdaous substances from wastewater and alternative approach for voltage production.

The remediation of di-(2-ethylhexyl) phthalate-contaminated sediments by water hyacinth biochar activation of calcium peroxide and its effect on cytotoxicity.

The presence of di-(2-ethylhexyl) phthalate (DEHP) in the aquatic systems, specifically marine sediments has attracted considerable attention worldwide, as it enters the food chain and adversely affects the aquatic environment and subsequently human health. This study reports an efficient carbocatalytic activation of calcium peroxide (CP) using water hyacinth biochar (WHBC) toward the efficient remediation of DEHP-contaminated sediments and offer insights into biochar-mediated cellular cytotoxicity, using a combination of chemical and bioanalytical methods. The pyrolysis temperature (300-900 °C) for WHBC preparation significantly controlled catalytic capacity. Under the experimental conditions studied, the carbocatalyst exhibited 94% of DEHP removal. Singlet oxygen (1O2), the major active species in the WHBC/CP system and electron-rich carbonyl functional groups of carbocatalyst, played crucial roles in the non-radical activation of CP. Furthermore, cellular toxicity evaluation indicated lower cytotoxicity in hepatocarcinoma cells (HepG2) after exposure to WHBC (25-1000 µg mL-1) for 24 h and that WHBC induced cell cycle arrest at the G2/M phase. Findings clearly indicated the feasibility of the WHBC/CP process for the restoration of contaminated sediment and contributing to understanding the mechanisms of cytotoxic effects and apoptotic of carbocatalyst on HepG2.

Reduction of hydrocarbon pollutants by hyacinth plants ( Eichhornia crassipes).

Background: The application of phytoremediation by utilizing plants has been used to control oil pollution in waters. One of the plants that can act as a phytoremediator is the hyacinth because this plant can reduce various pollutants including petroleum hydrocarbons. This study aims to study the reduction ability of petroleum hydrocarbons at different concentrations including improving water quality. Methods: This study consisted of one treatment (petroleum hydrocarbon) consisting of five factors with three replicates. The treatments consisted of 10 ppm (E1), 30 ppm (E2), 50 ppm (E3), 70 ppm (E4), 90 ppm (E5), and (E0) without aquatic plants as controls. The treatments were observed daily and measured from the first day (D-1), the seventh day (D-7), and the 14 th day (D-14). The water quality in each treatment was also measured, such as water temperature, pH, and dissolved oxygen. Results: The results showed that the hyacinth plant was able to reduce hydrocarbon in terms of total petroleum hydrocarbon (TPH) by 79% while it was only between 17-27% naturally without the hyacinth. The reduction of TPH in the water was in line with the decrease of chlorophyll in the leaves of hyacinths, and it was followed by the increase of dissolved oxygen in the water media. Conclusions: In conclusion, hyacinths can reduce petroleum hydrocarbons and they can improve the water quality as well.

Ecotoxicological effects and bioaccumulation in Eichhornia crassipes induced by long-term exposure to triclosan.

In this study, the ecotoxicological effects and bioaccumulation of triclosan (TCS) in Eichhornia crassipes (E. crassipes) were investigated with 28 d exposure experiments. The results showed that chlorophyll content was increased after 7 d exposure to 0.05-0.1 mg L-1 TCS, while it was inhibited significantly by 0.5 mg L-1 TCS after 21 d exposure. The concentrations of soluble protein in the leaves increased during the initial stage (7 d and 14 d), whereas they decreased during 21 d and 28 d. The concentrations of soluble protein in the roots gradually reduced during the exposure time. The antioxidant enzyme activities in roots decreased continually with the exposure time. However, the antioxidant enzyme (SOD and CAT) activities in leaves decreased after exposure longer than 14 d. Moreover, differentially expressed genes (DEGs) were observed in the root of E. crassipes after a 28 d exposure to 0.5 mg L-1 TCS, with 11023 DEGs down-regulated and 3947 DEGs up-regulated. 5 SOD down-regulated genes and 3 CAT down-regulated genes were identified from transport and catabolism in cellular processes. After 28 d exposure, the TCS content in roots and leaves stressed by 0.5 mg L-1 TCS were up to 13.04 µg g-1 and 1.97 µg g-1, respectively. SOD in leaves was negatively correlated with TCS content in leaves, CAT in roots was negatively correlated with TCS content in roots. These results provide experimental data to assess the ecological risk of TCS with long exposure in aquatic systems.

Comparative proteomic analysis of saline tolerant, phosphate solubilizing endophytic Pantoea sp., and Pseudomonas sp. isolated from Eichhornia rhizosphere.

Soil salinization is a major stress affecting crop production on a global scale. Application of stress tolerant plant growth promoting rhizobacteria (PGPR) in saline soil can be an ideal practice for improving soil fertility. Rhizospheric microbiota of stress tolerant Eichhornia crassipes was screened for saline tolerant phosphate solubilizing bacteria, and the two isolates showing maximum solubilization index at 1 M NaCl were subjected to further analyses. The isolates were identified as Pantoea dispersa and Pseudomonas aeruginosa. Among the two isolates, P. dispersa PSB1 showed better phosphorus (P) solubilization potential under saline stress (335 ± 30 mg/L) than P. aeruginosa PSB5 (200 ± 24 mg/L). The mechanisms of P-solubilization, such as the production of organic acids and phosphatase were found to be influenced negatively by saline stress. The adaptive mechanisms of the isolates to overcome salt stress were analyzed by protein profiling which revealed salt stress induced modulations in protein expression involved in amino acid biosynthesis, carbon metabolisms, chemotaxis, and stress responses. Survival mechanisms such as protein RecA, LexA repressor and iron-sulfur cluster synthesis were upregulated in both the organisms under saline stress. P. dispersa PSB1 showed improved defense mechanisms such as the production of osmotolerants, redox enzymes, and quorum quenchers under saline stress, which may explain its better P solubilization potential than the P. aeruginosa PSB5. This study emphasizes the need for molecular approaches like proteome analysis of PGPR for identifying novel traits like stress tolerance and plant growth promotion before developing them as biofertilizers and biocontrol formulations.