RÉSUMÉ
The interaction of the vanadyl (IV) cation with N-acetyl-D-galactosamine, D-galactosamine, and D-glucuronic acid has been investigated by electron absorption spectroscopy at different metal to ligand ratios and pH values. In the case of D-glucuronic acid, a more detailed study was undertaken, using differential IR spectroscopy in solution. The results show that the cation interacts with the two nitrogenated molecules only at higher pH values, generating 2:1 ligand to metal complexes in which coordination occurs through two pairs of deprotonated OH groups of the rings. In the case of D-glucuronic acid, the IR-measurements allowed a wider insight into the structural characteristics of the complexes generated in acidic media. The involvement of the glycosidic oxygen atom in coordination, is suggested at pH = 3.
Sujet(s)
Acétyl-galactosamine/composition chimique , Chondroïtines sulfate/métabolisme , Galactosamine/composition chimique , Glucuronates/composition chimique , Vanadates/métabolisme , Acétyl-galactosamine/métabolisme , Cations divalents , Chondroïtines sulfate/composition chimique , Galactosamine/métabolisme , Glucuronates/métabolisme , Acide glucuronique , Concentration en ions d'hydrogène , Ligands , Spectrophotométrie atomique , Spectrophotométrie IR , Vanadates/composition chimiqueRÉSUMÉ
We have induced acute hepatitis in rats with the amino sugar Galactosamine by i.p. injection. The development of the disease was controlled by measurements of several metabolites and enzymes in serum, and light and electronic microscopy. Tyrosine transaminase was induced by i.p. injection of Cortisol, that increases ten times enzyme activity in liver parenchyma of normal rats. This inductive phenomenon cannot be observed in animals with galactosamine hepatitis. We discuss the probable mechanism and their relationship with some forms of viral hepatitis in human beings.