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J Agric Food Chem ; 66(29): 7627-7632, 2018 Jul 25.
Article de Anglais | MEDLINE | ID: mdl-29944364

RÉSUMÉ

Sclerotinia sclerotiorum is responsible for the white mold of soybeans, and the difficulty to control the disease in Brazil is causing million-dollar damages. Stachybotrys levispora has shown activity against S. sclerotiorum. In our present investigation, we analyzed the chemical basis of this inhibition. Eight compounds were isolated, and using spectroscopic methods, we identified their structures as the known substances 7-dechlorogriseofulvin, 7-dechlorodehydrogriseofulvin, griseofulvin, dehydrogriseofulvin, 3,13-dihydroxy-5,9,11-trimethoxy-1-methylbenzophenone, griseophenone A, 13-hydroxy-3,5,9,11-tetramethoxy-1-methylbenzophenone, and 12-chloro-13-hydroxy-3,5,9,11-tetramethoxy-1-methylbenzophenone. Griseofulvin inhibited the mycelial growth of S. sclerotiorum at 2 µg mL-1. Thus, the antagonistic effect of S. levispora to S. sclerotiorum may well be due to the presence of griseofulvins. Our results stimulate new work on the biosynthesis of griseofulvins, to locate genes that encode key enzymes in these routes and use them to increase the production of these compounds and thus potentiate the fungicide effect of this fungus. S. levispora represents an agent for biocontrol, and griseofulvin represents a fungicide to S. sclerotiorum.


Sujet(s)
Ascomycota/effets des médicaments et des substances chimiques , Fongicides industriels/pharmacologie , Griséofulvine/pharmacologie , Maladies des plantes/prévention et contrôle , Stachybotrys/composition chimique , Ascomycota/physiologie , Brésil , Protéines fongiques/génétique , Protéines fongiques/métabolisme , Fongicides industriels/composition chimique , Fongicides industriels/métabolisme , Griséofulvine/composition chimique , Griséofulvine/métabolisme , Maladies des plantes/microbiologie , Glycine max/microbiologie , Stachybotrys/génétique , Stachybotrys/métabolisme
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