Elucidating the role of rice straw biochar in modulating Helianthus annuus L. antioxidants, secondary metabolites and soil post-harvest characteristics in different types of microplastics.

The emergence of microplastics (MPs) as pollutants in agricultural soils is increasingly alarming, presenting significant threats to soil ecosystems. Given the widespread contamination of ecosystems by various types of MPs, including polystyrene (PS), polyvinyl chloride (PVC), and polyethylene (PE), it is crucial to understand their effects on agricultural productivity. The present study was conducted to investigate the effects of different types of MPs (PS, PVC, and PE) on various aspects of sunflower (Helianthus annuus L.) growth with the addition of rice straw biochar (RSB). This study aimed to examine plant growth and biomass, photosynthetic pigments and gas exchange characteristics, oxidative stress indicators, and the response of various antioxidants (enzymatic and non-enzymatic) and their specific gene expression, proline metabolism, the AsA-GSH cycle, cellular fractionation in the plants and post-harvest soil properties. The research outcomes indicated that elevated levels of different types of MPs in the soil notably reduced plant growth and biomass, photosynthetic pigments, and gas exchange attributes. Different types of MPs also induced oxidative stress, which caused an increase in various enzymatic and non-enzymatic antioxidant compounds, gene expression and sugar content; notably, a significant increase in proline metabolism, AsA-GSH cycle, and pigmentation of cellular components was also observed. Favorably, the addition of RSB significantly increased plant growth and biomass, gas exchange characteristics, enzymatic and non-enzymatic compounds, and relevant gene expression while decreasing oxidative stress. In addition, RSB amendment decreased proline metabolism and AsA-GSH cycle in H. annuus plants, thereby enhancing cellular fractionation and improving post-harvest soil properties. These results open new avenues for sustainable agriculture practices and show great potential for resolving the urgent issues caused by microplastic contamination in agricultural soils.

Strengthen sunflowers resilience to cadmium in saline-alkali soil by PGPR-augmented biochar.

In the center of the Nile Delta in Egypt, the Kitchener drain as the primary drainage discharges about 1.9 billion m3 per year of water, which comprises agricultural drainage (75 %), domestic water (23 %), and industrial water (2 %), to the Mediterranean Sea. Cadmium (Cd) stands out as a significant contaminant in this drain; therefore, this study aimed to assess the integration of biochar (0, 5, and 10 ton ha-1) and three PGPRs (PGPR-1, PGPR-2, and PGPR-3) to alleviate the negative impacts of Cd on sunflowers (Helianthus annuus L.) in saline-alkali soil. The treatment of biochar (10 ton ha-1) and PGPR-3 enhanced the soil respiration, dehydrogenase, nitrogenase, and phosphatase activities by 137 %, 129 %, 326 %, and 127 %, while it declined soil electrical conductivity and available Cd content by 31.7 % and 61.3 %. Also, it decreased Cd content in root, shoot, and seed by 55.3 %, 50.7 %, and 92.5 %, and biological concentration and translocation factors by 55 % and 5 %. It also declined the proline, lipid peroxidation, H2O2, and electrolyte leakage contents by 48 %, 94 %, 80 %, and 76 %, whereas increased the catalase, peroxidase, superoxide dismutase, and polyphenol oxidase activities by 80 %, 79 %, 61 %, and 116 %. Same treatment increased seed and oil yields increased by 76.1 % and 76.2 %. The unique aspect of this research is its investigation into the utilization of biochar in saline-alkali soil conditions, coupled with the combined application of biochar and PGPR to mitigate the adverse effects of Cd contamination on sunflower cultivation in saline-alkali soil.

Mitigation of drought-induced stress in sunflower (Helianthus annuus L.) via foliar application of Jasmonic acid through the augmentation of growth, physiological, and biochemical attributes.

Drought stress poses a significant threat to agricultural productivity, especially in areas susceptible to water scarcity. Sunflower (Helianthus annuus L.) is a widely cultivated oilseed crop with considerable potential globally. Jasmonic acid, a plant growth regulator, plays a crucial role in alleviating the adverse impacts of drought stress on the morphological, biochemical, and physiological characteristics of crops. Experimental detail includes sunflower varieties (Armani Gold, KQS-HSF-1, Parsun, and ESFH-3391), four drought stress levels (0, 25%, 50%, and 75% drought stress), and three levels (0, 40ppm, 80ppm) of jasmonic acid. The 0% drought stress and 0ppm jasmonic acid were considered as control treatments. The experimental design was a completely randomized design with three replicates. Drought stress significantly reduced the growth in all varieties. However, the exogenous application of jasmonic acid at concentrations of 40ppm and 80ppm enhanced growth parameters, shoot and root length (1.93%, 19%), shoot and root fresh weight (18.5%, 25%), chlorophyll content (36%), photosynthetic rate (22%), transpiration rate (40%), WUE (20%), MDA (6.5%), Phenolics (19%), hydrogen peroxide (7%) proline (28%) and glycine betaine (15-30%) under water-stressed conditions, which was closely linked to the increase in stomatal activity stimulated by jasmonic acid. Furthermore, JA 80 ppm was found to be the most appropriate dose to reduce the effect of water stress in all sunflower varieties. It was concluded that the foliar application of JA has the potential to enhance drought tolerance by improving the morphological, biochemical, and physiological of sunflower.

Uptake, biotransformation and physiological response of TBBPA derivatives in Helianthus annus.

Tetrabromobisphenol A (TBBPA) and its derivatives are widely used as brominated flame retardants. Because of their high production and wide environment distribution, TBBPA derivatives have increased considerable concern. Previous studies have primarily focused on TBBPA, with limited information available on its derivative. In this study, we investigated the uptake, biotransformation and physiological response of two derivatives, Tetrabromobisphenol A bis(allyl ether) (TBBPA BAE) and Tetrabromobisphenol A bis(2,3-dibromopropylether) (TBBPA BDBPE), in Helianthus annus (H. annus) through a short-term hydroponic assay. The results revealed that H. annus could absorb TBBPA BAE and TBBPA BDBPE from solution, with removal efficiencies of 98.33 ± 0.5% and 98.49 ± 1.56% after 10 days, respectively, which followed first-order kinetics. TBBPA BAE was absorbed, translocated and accumulated while TBBPA BDBPE couldn't be translocated upward due to its high hydrophobicity and low solubility. The concentrations of TBBPA derivatives in plants peaked within 72 h, and then decreased. We identified twelve metabolites resulting from ether bond breakage, debromination, and hydroxylation in H. annus. The high-level TBBPA BAE suppressed the growth and increased malondialdehyde (MDA) content of H. annus, while TBBPA BDBPE didn't pose a negative effect on H. annus. TBBPA BAE and TBBPA BDBPE increased the activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), with higher levels of these enzymes activity found in high concentration treatments. Contrastingly, TBBPA BAE exhibited higher toxicity than TBBPA BDBPE, as indicated by greater antioxidant enzyme activity. The findings of this study develop better understanding of biotransformation mechanisms of TBBPA derivatives in plants, contributing to the assessment of the environmental and human health impacts of these contaminants.

Seed pretreatment with brassinosteroids stimulates sunflower immunity against parasitic weed (Orobanche cumana) infection.

Broomrape (Orobanche cumana) negatively affects sunflower, causing severe yield losses, and thus, there is a need to control O. cumana infestation. Brassinosteroids (BRs) play key roles in plant growth and provide resilience to weed infection. This study aims to evaluate the mechanisms by which BRs ameliorate O. cumana infection in sunflower (Helianthus annuus). Seeds were pretreated with BRs (1, 10, and 100 nM) and O. cumana inoculation for 4 weeks under soil conditions. O. cumana infection significantly reduced plant growth traits, photosynthesis, endogenous BRs and regulated the plant defence (POX, GST), BRs signalling (BAK1, BSK1 to BSK4) and synthesis (BRI1, BR6OX2) genes. O. cumana also elevated the levels of malondialdehyde (MDA), hydroxyl radical (OH-), hydrogen peroxide (H2O2) and superoxide (O2 •-) in leaves/roots by 77/112, 63/103, 56/97 and 54/89%, as well as caused ultrastructural cellular damages in both leaves and roots. In response, plants activated a few enzymes, superoxide dismutase (SOD), peroxidase (POD) and reduced glutathione but were unable to stimulate the activity of ascorbate peroxidase (APX) and catalase (CAT) enzymes. The addition of BRs (especially at 10 nM) notably recovered the ultrastructural cellular damages, lowered the production of oxidative stress, activated the key enzymatic antioxidants and induced the phenolic and lignin contents. The downregulation in the particular genes by BRs is attributed to the increased resilience of sunflower via a susceptible reaction. In a nutshell, BRs notably enhanced the sunflower resistance to O. cumana infection by escalating the plant immunity responses, inducing systemic acquired resistance, reducing oxidative or cellular damages, and modulating the expression of BR synthesis or signalling genes.

Hydrogen sulfide-mitigated salinity stress impact in sunflower seedlings was associated with improved photosynthesis performance and osmoregulation.

BACKGROUND: Salinity is one major abiotic stress affecting photosynthesis, plant growth, and development, resulting in low-input crops. Although photosynthesis underlies the substantial productivity and biomass storage of crop yield, the response of the sunflower photosynthetic machinery to salinity imposition and how H2S mitigates the salinity-induced photosynthetic injury remains largely unclear. Seed priming with 0.5 mM NaHS, as a donor of H2S, was adopted to analyze this issue under NaCl stress. Primed and nonprime seeds were established in nonsaline soil irrigated with tape water for 14 d, and then the seedlings were exposed to 150 mM NaCl for 7 d under controlled growth conditions. RESULTS: Salinity stress significantly harmed plant growth, photosynthetic parameters, the structural integrity of chloroplasts, and mesophyll cells. H2S priming improved the growth parameters, relative water content, stomatal density and aperture, photosynthetic pigments, photochemical efficiency of PSII, photosynthetic performance, soluble sugar as well as soluble protein contents while reducing proline and ABA under salinity. H2S also boosted the transcriptional level of ribulose 1,5-bisphosphate carboxylase small subunit gene (HaRBCS). Further, the transmission electron microscope showed that under H2S priming and salinity stress, mesophyll cells maintained their cell membrane integrity and integrated chloroplasts with well-developed thylakoid membranes. CONCLUSION: The results underscore the importance of H2S priming in maintaining photochemical efficiency, Rubisco activity, and preserving the chloroplast structure which participates in salinity stress adaptation, and possibly sunflower productivity under salinity imposition. This underpins retaining and minimizing the injury to the photosynthetic machinery to be a crucial trait in response of sunflower to salinity stress.

Comparative investigation on chemical and green synthesized titanium dioxide nanoparticles against chromium (VI) stress eliciting differential physiological, biochemical, and cellular attributes in Helianthus annuus L.

Nanotechnology is a new scientific area that promotes unique concepts to comprehend the optimal mechanics of nanoparticles (NPs) in plants under heavy metal stress. The present investigation focuses on effects of synthetic and green synthesized titanium dioxide nanoparticles (TiO2 NPs and gTiO2 NPs) against Cr(VI). Green TiO2 NPs have been produced from plant leaf extract (Ricinus communis L.). Synthesis was confirmed employing an array of optical spectroscopic and electron microscopic techniques. Chromium strongly accelerated H2O2 and MDA productions by 227 % and 266 % at highest chromium concentration (60 mg/kg of soil), respectively, and also caused DNA damage, and decline in photosynthesis. Additionally, anomalies were observed in stomatal cells with gradual increment in chromium concentrations. Conversely, foliar applications of TiO2 NPs and gTiO2 NPs considerably mitigated chromium stress. Sunflower plants treated with modest amounts of green TiO2 NPs had significantly better growth index compared to chemically synthesized ones. Principal component analysis highlighted the variations among photosynthetic attributes, oxidative stress markers, and antioxidant defense systems. Notably, gTiO2 supplementation to the Cr(VI) strained plants minimized PC3 production which is a rare report so far. Conclusively, gTiO2 NPs have been identified to be promising nano-based nutrition resource for farming applications.

Methionine-induced regulation of growth, secondary metabolites and oxidative defense system in sunflower (Helianthus annuus L.) plants subjected to water deficit stress.

Optimum water availability at different growth stages is one the major prerequisites of best growth and yield production of plants. Exogenous application of plant growth regulators considered effective for normal functioning of plants under water-deficit conditions. A study was conducted to examine the influence of exogenously applied L-methionine on sunflower (Helianthus annuus L.) plants grown under water-deficit conditions. Twenty-five-day old seedlings of four sunflower cultivars, FH331, FH572, FH652 and FH623 were exposed to control (100% F.C.) and drought stress (60% F.C.) conditions. After 30-day of drought stress, L-methionine (Met; 20 mg/L) was applied as a foliar spray to control and drought stressed plants. Water deficit stress significantly reduced shoot fresh and dry weights shoot and root lengths, and chlorophyll a content in all four cultivars. While a significant increase was observed due to water deficiency in relative membrane permeability (RMP), malondialdehyde (MDA), total soluble proteins (TSP), total soluble sugars (TSS), ascorbic acid (AsA) and activity of peroxidase (POD). Although, exogenously applied Met was effective in decreasing RMP, MDA and H2O2 contents, it increased the shoot fresh weight, shoot length, chlorophyll a, chlorophyll a/b ratio, proline contents and the activities of SOD, POD and CAT enzymes in all four cultivars under water deficit stress. No change in AsA and total phenolics was observed due to foliar-applied Met under water stress conditions. Of all sunflower cultivars, cv. FH-572 was the highest and cv. FH-652 the lowest of all four cultivars in shoot fresh and dry weights as well as shoot length under drought stress conditions. Overall, foliar applied L-methionine was effective in improving the drought stress tolerance of sunflower plants that was found to be positively associated with Met induced improved growth attributes and reduced RMP, MDA and H2O2 contents under water deficit conditions.

Potassium fertilization improves growth, yield and seed quality of sunflower (Helianthus annuus L.) under drought stress at different growth stages.

Water scarcity is a major concern for sunflower production in the semi-arid and arid regions of the world. Potassium (K) application has been found effective to alleviate the influence of drought stress; however, the impact of drought stress on seed quality of sunflower has not been reported frequently. Therefore, a field experiment was performed to determine the optimum K requirement for mitigating the adverse effects of water stress and improving growth and seed quality of spring-planted sunflower. Sunflower plants were exposed to water stress at different growth stages, i.e., Io = no stress (normal irrigation), I1 = pre-anthesisi stress (irrigation skipped at pre-anthesis stage), I2 = anthesis stress (irrigation skipped at anthesis stage) and I3 = post-anthesis stress (irrigation skipped at post-anthesis stage). Potassium was applied at four different rates, i.e., Ko = 0, K1 = 50, K2 = 100 and K3 = 150 kg ha-1. The results revealed that water stress at pre- and post-anthesis stages significantly reduced plant height, head diameter, number of achenes, oleic acid contents, and phosphorus (P) uptake. However, pre-anthesis stress improved linoleic acid contents. Treatment IoK3 (stress-free with 150 kg ha-1 K) was optimum combination for 1000-achene weight, biological and achene yields, oil contents, protein contents, and N and P uptake. Results indicated that a higher amount of K and irrigation resulted in higher yield, whereas yield and yield components decreased with early-stage water stress. Nevertheless, potassium application lowered the impacts of waters stress compared to no application. Keeping in view these results, it is recommended that sunflower must be supplied 150 kg ha-1 K in arid and semi-arid regions to achieve higher yield and better seed quality.

Genome-wide investigation of bHLH genes and expression analysis under different biotic and abiotic stresses in Helianthus annuus L.

The basic helix-loop-helix (bHLH) transcription factors play important roles in many processes such as plant growth, metabolism and response to biotic/abiotic stresses. Sunflower (Helianthus annuus) is a major oil crop, cultivated throughout the world. However, no systematic characterization of bHLH gene members in sunflower (HabHLH) and their functions involved in drought, cadmium tolerance and Orobanche cumana resistance has been reported yet. In this study, 183 HabHLH genes were identified and named according to their chromosomal locations. We classified these proteins into 21 subfamilies by phylogenetic tree analysis. Subsequently, DNA-binding patterns, sequence analysis, duplication analysis and gene structures were analyzed. All of the HabHLH genes were randomly distributed on 17 chromosomes, and 10 pairs of tandem duplicated genes and one pair of segmental duplicated genes were detected in the HabHLH family. Among the duplicated gene pairs, eight pairs of HabHLH genes suffer from positive selection. Moreover, qRT-PCR results revealed significant up-regulated expression of HabHLH024 gene in response to both abiotic (cadmium, drought) and biotic (Orobanche cumana) stresses, suggesting its important functions in response to different stresses. Therefore, HabHLH024 would be the potential candidate gene for the sunflower tolerance breeding.

Gallic acid improves the antioxidant ability against cadmium toxicity: Impact on leaf lipid composition of sunflower (Helianthus annuus) seedlings.

In the present work, the effect of seed pre-soaking with gallic acid (GA; 3,4,5-triphydroxyl-benzoic acid) in conferring subsequent tolerance to Cd stress in sunflower (Helianthus annuus) seedlings was investigated. Exposing sunflower seedlings to increasing Cd concentrations (5, 10 and 20 µM) caused a gradual decrease in root and shoot biomass and increased the metal accumulation in both organs. Seed pretreatment with 75 µM GA significantly restricted Cd uptake, markedly alleviated Cd-induced plant growth inhibition, and mitigated the oxidative damages caused by this metal, as compared to plants directly exposed to Cd. GA pre-soaking prior to Cd stress also enhanced catalase, ascorbate peroxidase and glutathione reductase activities, while inhibiting that of superoxide dismutase. This was associated with increased levels of total thiols and glutathione along with a decreased level of oxidized glutathione in leaves. Moreover, GA pre-soaking led to changes in leaf fatty acid composition of seedlings challenged with Cd, as evidenced by the higher total lipid content and lipid unsaturation degree. As a whole, this study provides strong arguments highlighting the potential role of GA as a growth promoter for sunflower seedlings submitted to Cd stress, notably by boosting the antioxidant defense system and improving leaf membrane stability.

Activated sludge treatment for promoting the reuse of a synthetic produced water in irrigation.

Large volumes of produced water are generated as a byproduct in activities of oil and gas exploitation, which can be reused in agriculture after a treatment process. Activated sludge treatment has been successfully used to remove oil from wastewater, but systematic studies on the toxicity of this effluent using this treatment are scarce in the literature. In this study, it was investigated the performance of an activated sludge system in the treatment of a synthetic produced water under different initial conditions in terms of salinity and oil and grease concentration. Furthermore, it was evaluated this effluent phytotoxicity in the germination, and seedling and plant growths of sunflower and corn seeds using untreated and treated synthetic produced water. Results revealed the activated sludge effectiveness in oil and grease and salinity removal from produced water, viz. high removal efficiency of 99.01 ± 0.28 and 91.07 ± 0.39%., respectively. Untreated produced water showed considerable toxic effects on the germination (74.67 ± 2.31% and 82.67 ± 2.31 for sunflower and corn seeds, respectively) and growth stages of sunflower and corn seed plants. The germination percentage was approximately 100% for both types of seed. The seedling and plant growth of the two seeds irrigated with treated produced water had similar performance when used tap water. These results highlighted the potential reuse as an unconventional water resource for plant irrigation of the synthetic produced water treated by an activated sludge process, which technology has showed high removal performance of salinity and oil.

A three-year large scale study on the risk of honey bee colony exposure to blooming sunflowers grown from seeds treated with thiamethoxam and clothianidin neonicotinoids.

Despite the restriction of the use of neonicotinoids in the EU, including thiamethoxam and clothianidin, the debate over their risk on honey bees has not been fully settled. This study presents results of a three-year study working with 180 honey bee colonies in ten replicates. Colonies were sorted into three treatments (60 colonies per treatment) exposed to sunflower blooms grown from seeds treated with thiamethoxam, clothianidin and a non-treated control. Each colony was assessed at six moments: one before to exposition to sunflower, two during the exposition (short-time risk), two after exposition (medium-time risk) and one after wintering (long-time risk). The health and development of the colonies were assessed by monitoring adult bee population, brood development, status of the queen, food reserves and survival. No significant difference among treatments when raw data was considered. However, when evolution from initial status of the colony was evaluated, a significant difference was observed from the first week of exposure to sunflower blooms. In this period, the number of adult bees and the amount of brood were slightly lower in the bee hives exposed to neonicotinoids, although such differences disappeared in subsequent evaluations. The concentration of residues in samples of beebread and adult bees was at the level of ng·g-1. Magnitude of the effect of the treatment factor on the variability of colony health and development related parameters was low. The most important factor was the hive, followed by the replicate and year, and to a lesser extent the initial strength of the colonies.

Different methods of silicon application attenuate salt stress in sorghum and sunflower by modifying the antioxidative defense mechanism.

Soil salinization is the most common abiotic stress limiting agricultural productivity worldwide. Recent research has suggested that the application of silicon (Si) has beneficial effects against salt stress in sorghum (Sorghum bicolor L. Moench) and sunflower (Helianthus annuus L.) by regulating the antioxidant system, mineral nutrients, and other important mechanisms. However, whether these effects can be achieved through foliar application of Si, or whether Si application affects Si-accumulating (e.g., sorghum), and intermediate-Si-accumulating (e.g., sunflower) plant species differently, remains unclear. This study investigated different methods of Si application in attenuating the detrimental effects of salt stress, based on the biological responses of two distinct species of Si accumulators, under greenhouse conditions. Two pot experiments were designed as a factorial (2 × 4), randomized complete blocks design (RCBD) with control and salt-stress groups (0 and 100 mmol.L-1 NaCl), and four Si-treatment groups: control (no Si), foliar application (28.6 mmol.L-1), root application (2 mmol.L-1), and combined foliar and root applications. Our results showed that the harmful effects of salt stress were attenuated by Si treatments in both plant species, which decreased Na+ uptake and lipid peroxidation, and increased Si and K+ uptake, relative leaf water content, antioxidant enzyme activities, leaf area, and shoot dry matter. These results were more prominent when Si was applied via nutrient solution in the sorghum plants, and the combined foliar and root applications of Si in sunflower plants. In addition, foliar application of Si alone is an efficient alternative in attenuating the effects of salinity in both plant species when Si is not available in the growth medium. These results suggest that the Si application method plays an important role in Na+ detoxification by modifying the antioxidative defense mechanism, which could actively mediate some important physiological and biochemical processes and helps to increase the shoot dry matter production in sorghum and sunflower plants under salt stress.

Phytohormones producing rhizobacterium alleviates chromium toxicity in Helianthus annuus L. by reducing chromate uptake and strengthening antioxidant system.

Contamination of agricultural land with heavy metal is a serious biological and environmental issue. Such threat can be challenged by exploring the plant symbiotic microbes that can improve plant growth through phyto-hormones secretion and chromate chelation. In the current study, chromate resistant rhizospheric Staphylococcus arlettae strain MT4 was isolated from the rhizosphere of Malvestrum tricuspadatum L. The strain showed potential to secrete phytohormones and plant growth promoting secondary metabolites under induced chromate stress, making it a best suitable candidate in chromate stress alleviation. Moreover, the rhizobacterium MT4 significantly promoted the net assimilation and relative growth rate of sunflower grown in the presence of chromate (100 ppm). Chromate stress alleviation strategy of MT4 strain was three-fold. MT4 alleviated chromate stress and promoted the sunflower growth by suppressing the chromate intake by the host, modulating phytohormones and strengthening of the host's antioxidant system. The improved antioxidant system was confirmed by noticing lower ROS accumulation and improved ROS scavenging, lower peroxidase activity and higher accumulation of phenols and flavonoids.

Growth, Yield and Biochemical Impact of Anti-transpirants on Sunflower Plant Grown under Water Deficit.

BACKGROUND AND OBJECTIVE: Climate change affecting on weather in many different ways especially drought and temperature rise. This will drastically down plant production, if not start looking for another source to increase water productivity to cope up with water stress conditions. In this study efforts were conducted to interpret the use of anti-transpirants to conserving irrigation water, aiding plant survival under dry conditions and protecting plant against drought stress. MATERIALS AND METHODS: Two field experiments were carried out during 2017 and 2018 successive growing summer seasons at the experimental farm of National Research Centre, Nubaria, El-Beheira Governorate, Egypt. Anti-transparent ,i.e., chitosan (100 and 150 mg L-1), calcium carbonate (5 and 10 g L-1), salicylic acid (200 and 300 mg L-1) were foliar sprayed on sunflower plants grown under two water levels (normal 100% and deficit 50%). RESULTS: The results showed that water stress decreased growth criteria, photosynthetic pigments, osmoprotectants, yield components, oil and carbohydrate (%) as compared to 100% of irrigation requirements. Meanwhile, water deficit induced significant increases in (proline). Foliar treatments of sunflower plant with chitosan, calcium carbonate, salicylic acid increased growth criteria, yield components, photosynthetic pigments, total soluble sugars, proline and free amino acid as compared to control plant. Data also illustrated that, all used treatment improved seed yield, oil and carbohydrate% of sunflower plants. CONCLUSION: Generally, it could be concluded that 10 g L-1 CaCO3 and 300 mg L-1 SA at 50% level of water irrigation could be recommended for sunflower plant grown under similar field conditions in order to get optimum yield and to save irrigation water.

Effect of Fe3O4 nanoparticles on germination of seeds and concentration of elements in Helianthus annuus L. under constant magnetic field.

The aim of the study was to investigate the effect of the Fe3O4 nanoparticles (Fe-NPs) on the germination of sunflower seeds, early growth of seedlings and the concentration of selected elements in seedlings. The influence of constant magnetic fields in systems with and without Fe-NPs was investigated. Experiments were done on seeds subjected to germination under constant magnetic field (0 (control), 5, 25 and 120 mT) for 7 days in the presence of solution containing 0, 50 or 500 ppm Fe-NPs. No significant effect of Fe-NPs and the magnetic field on germination of seeds and the growth of seedlings has been demonstrated. In most cases, a decrease in germination parameters was observed. For the majority of samples the relative decrease in the concentrations of elements was demonstrated mainly for samples without Fe-NPs. Interestingly, a significant decrease in the concentrations of trivalent (including iron - Fe) and toxic elements in samples containing Fe-NPs in relation to control samples was observed. The authors suggest that in this case the binding (adsorption) of these elements in the roots and seeds of the sunflower by Fe-NPs took place. This explains the lower iron content in seedlings than in seeds prior to sowing.

First case of glyphosate resistance in weedy sunflower (Helianthus annuus).

BACKGROUND: Weedy sunflower (Helianthus annuus L.) is a troublesome weed in row-crop production fields in South Texas. Populations with suspected resistance to glyphosate were evaluated with 1X and 4X rates (X = 868 g ae ha-1 ) of the herbicide, followed by a dose-response assay of the most resistant population. Molecular studies were conducted to determine if target-site mechanisms were responsible for resistance in these populations. Additionally, field experiments were conducted at two locations (Somerville and Granger, TX) to evaluate the effectiveness of different tank-mix combinations in controlling naturally infesting glyphosate-resistant (GR) weedy sunflower populations in GR corn. RESULTS: In a study conducted in the growth chamber, seven of the 11 tested populations survived up to the 4X rate of glyphosate. The most-resistant population (TX15-11) was 29-fold more resistant to glyphosate, compared to the susceptible standard. In resistant populations, 5-21 more copies of the EPSPS gene were observed compared to the susceptible standard. In the field studies, tank-mix applications of glyphosate + halosulfuron-methyl, glyphosate + prosulfuron, glyphosate + a premix of halosulfuron-methyl and dicamba or glyphosate + a premix of diflufenzopyr and dicamba effectively controlled GR weedy sunflower populations. CONCLUSION: Glyphosate-resistance was observed in 81% of the putative resistant weedy sunflower populations tested in this study. Resistance in these populations was conferred primarily by amplification of the EPSPS gene. Effective control of GR weedy sunflower can be achieved by tank-mixes tested in the current study, which provides acceptable levels of crop safety. © 2020 Society of Chemical Industry.

Green synthesis of sulfur nanoparticles using Ocimum basilicum leaves and its prospective effect on manganese-stressed Helianthus annuus (L.) seedlings.

A novel green approach was utilized to fabricate sulfur nanoparticles (SNPs) with the aid of Ocimum basilicum leaves extract. The effective formation of the synthesized SNPs was examined and approved using UV-visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) spectroscopy. The average particle size was 23 nm with spherical shape and crystalline in nature. In the pot experiment, the synthesized SNPs were applied with different concentrations (12.5, 25, 50, 100 and 200 µM) as pre-soaking to Helianthus annuus seeds and irrigated with 100 mM MnSO4. As a result of manganese (Mn) exposure, the harvested 14-day sunflower seedlings showed a significant decline in the growth parameters (shoot length, leaf area and the relative water content of both shoot and root), photosynthetic pigments, mineral content (N, P, K, Ca, and Mg), and protein content compared to the control. The root length, electrolyte leakage, Na and Mn levels, metabolites content (amino acids, protein, glycine betaine, proline, and cysteine) were greatly raised as affected by Mn stress. Mn toxicity reduction using SNPs was demonstrated, as the medium doses enhanced seedlings growth, photosynthetic pigments, and mineral nutrients. Application of SNPs decreased Mn uptake and enhanced S metabolism through increasing cysteine level. Likewise, SNPs elevated seedlings water content and eliminated physiological drought via increasing osmolytes such as amino acids and proline. It can be concluded that green-synthesized SNPs had the potential to limit the deleterious effects of Mn stress.

Protective effect of potassium and chitosan supply on growth, physiological processes and antioxidative machinery in sunflower (Helianthus annuus L.) under drought stress.

Drought stress is one of the extreme effects of climate change causing large losses in production of crop plants. The risk of recurrent droughts has increased in next decades hence, the development of shot-gun, inexpensive and effective approaches is essential to ensure high yield of crops in drought-prone areas of the world. Exogenous application of nutrients such as potassium (K) has been reported to increase abiotic resistance and improve yield in crops however, knowledge regarding interaction of K with osmoprotectants like chitosan (Ct) still remains elusive. Here, we report the effects of individual or combined K (using K2SO4 as a source) or Ct application on growth, physiological processes and antioxidative defense system of sunflower under drought stress. At first, various doses of K (0, 5, 10, 15, 20, 25 g/l) and Ct (0, 0.1, 0.2, 0.3, 0.4, 0.5 g/l) were foliar applied to evaluate their role in improving plant biomass, water status and total chlorophyll in drought-induced seedlings of sunflower. The optimized K (11.48 g/l) and Ct (0.28 g/l) doses were further tested in second experiment to understand the underlying mechanisms of drought tolerance. Foliar K + Ct spray markedly enhanced the leaf gas exchange characteristics, increased proline, soluble proteins, and free amino acids, upregulated antioxidant defense system and helped to maintain plant water status in sunflower exposed to drought stress. The impact of drought stress was more pronounced at vegetative than reproductive stage and positive effects of combined K and Ct application were related to improved physiological and metabolic processes to improve yield and quality of sunflower under drought stress.