RÉSUMÉ
The health benefits of nut consumption have been extensively demonstrated in observational studies and intervention trials. Besides the high nutritional value, countless evidences show that incorporating nuts into the diet may contribute to health promotion and prevention of certain diseases. Such benefits have been mostly and certainly attributed not only to their richness in healthy lipids (plentiful in unsaturated fatty acids), but also to the presence of a vast array of phytochemicals, such as polar lipids, squalene, phytosterols, tocochromanols, and polyphenolic compounds. Thus, many nut chemical compounds apply well to the designation "nutraceuticals," a broad umbrella term used to describe any food component that, in addition to the basic nutritional value, can contribute extra health benefits. This contribution analyses the general chemical profile of groundnut and common tree nuts (almond, walnut, cashew, hazelnut, pistachio, macadamia, pecan), focusing on lipid components and phytochemicals, with a view on their bioactive properties. Relevant scientific literature linking consumption of nuts, and/or some of their components, with ameliorative and/or preventive effects on selected diseases - such as cancer, cardiovascular, metabolic, and neurodegenerative pathologies - was also reviewed. In addition, the bioactive properties were analyzed in the light of known mechanistic frameworks.
Sujet(s)
Compléments alimentaires , Juglans , Noix , Composés phytochimiques , Pistacia , Noix/composition chimique , Composés phytochimiques/analyse , Composés phytochimiques/pharmacologie , Humains , Compléments alimentaires/analyse , Juglans/composition chimique , Pistacia/composition chimique , Lipides/analyse , Valeur nutritive , Anacardium/composition chimique , Macadamia/composition chimique , Corylus/composition chimique , Phytostérols/analyse , Carya/composition chimique , Prunus dulcis/composition chimique , Maladies cardiovasculaires/prévention et contrôleRÉSUMÉ
Efforts are ongoing to utilise agricultural waste to achieve a full resource use approach. Bambara groundnut is an important crop widely grown in the sub-Saharan Africa with potential future importance because of its resilience to thrive under heightened weather uncertainty and widespread droughts that have challenged food security. After harvesting, the edible nuts are separated from the shells which are discarded as waste. Therefore, this research is aimed at characterising the chemical composition and the structural properties of Bambara groundnut shells (BGS) in view of their potential application as a biomass for different bio-products. The chemical composition of BGS was found to be 42.4% cellulose, 27.8% hemicellulose, 13% lignin and 16.8% extractives. Proximate analysis showed a high amount of volatile matter (69.1%) and low moisture (4.4%). XRD analysis confirmed crystallinity of cellulose I polymer and FTIR analysis observed functional groups of lignocellulosic compounds. Thermal stability, maximum degradation temperature and activation energy were found to be 178.5 °C, 305.7 °C and 49.4 kJ/mol, respectively. Compared to other nutshells, BGS were found to have a relatively high amount of cellulose and crystallinity that may result in biocomposites with improved mechanical properties.
Sujet(s)
Biomasse , Vigna , Vigna/composition chimique , Lignine/composition chimique , Lignine/analyse , Cellulose/composition chimique , Cellulose/analyse , Déchets/analyse , Noix/composition chimique , Biocarburants , PolyosidesRÉSUMÉ
BACKGROUND: Betel quid and its major ingredient, areca nut, are recognized by IARC as major risk factors in oral cancer development. Areca nut extract (ANE) exposure has been linked to OPMD progression and malignant transformation to OSCC. However, the detailed mechanism through which ANE acts on other cell types in the oral microenvironment to promote oral carcinogenesis remains elusive. METHODS: Immunoprofiling of macrophages associated with OPMD and OSCC was carried out by immunohistochemical and immunofluorescence staining. Phosphokinase and cytokine arrays and western blotting were performed to determine the underlying mechanisms. Transwell assays were used to evaluate the migration-promoting effect of ANE. Hamster model was finally applied to confirm the in vivo effect of ANE. RESULTS: We reported that M2 macrophages positively correlated with oral cancer progression. ANE induced M2 macrophage differentiation, CREB phosphorylation and VCAM-1 secretion and increased mitochondrial metabolism. Conditioned medium and VCAM-1 from ANE-treated macrophages promoted migration and mesenchymal phenotypes in oral precancer cells. In vivo studies showed that ANE enhanced M2 polarization and related signaling pathways in the oral buccal tissues of hamsters. CONCLUSION: Our study provides novel mechanisms for areca nut-induced oral carcinogenesis, demonstrating that areca nut promotes M2 macrophage differentiation and secretion of oncogenic cytokines that critically activate malignant transformation of oral premalignant cells.
Sujet(s)
Areca , Différenciation cellulaire , Transformation cellulaire néoplasique , Macrophages , Tumeurs de la bouche , Animaux , Areca/effets indésirables , Areca/composition chimique , Transformation cellulaire néoplasique/métabolisme , Tumeurs de la bouche/anatomopathologie , Tumeurs de la bouche/métabolisme , Humains , Macrophages/métabolisme , Cricetinae , Modèles animaux de maladie humaine , Noix , Mâle ,RÉSUMÉ
Turkey is the leading producer of hazelnuts, contributing to 62% of the total global production. Among 18 distinct local hazelnut cultivars, Giresun Tombul is the only cultivar that has received Protected Designation of Origin denomination from the European Comission (EC). However, there is currently no practical objective method to ensure its geographic origin. Therefore, in this study NIR and Raman spectroscopy, along with chemometric methods, such as principal component analysis, PLS-DA (partial least squares-discriminant analysis), and SVM-C (support vector machine-classification), were used to determine the geographical origin of the Giresun Tombul hazelnut cultivar. For this purpose, samples from unique 118 orchards were collected from eight different regions in Turkey during the 2021 and 2022 growing seasons. NIR and Raman spectra were obtained from both the shell and kernel of each sample. The results indicated that hazelnut samples exhibited distinct grouping tendencies based on growing season regardless of the spectroscopic technique and sample type (shell or kernel). Spectral information obtained from hazelnut shells demonstrated higher discriminative power concerning geographical origin compared to that obtained from hazelnut kernels. The PLS-DA models utilizing FT-NIR (Fourier transform near-infrared) and Raman spectra for hazelnut shells achieved validation accuracies of 81.7% and 88.3%, respectively, while SVM-C models yielded accuracies of 90.9% and 86.3%. It was concluded that the lignocellulosic composition of hazelnut shells, indicative of their geographic origin, can be accurately assessed using FT-NIR and Raman spectroscopy, providing a nondestructive, rapid, and user-friendly method for identifying the geographical origin of Giresun Tombul hazelnuts. PRACTICAL APPLICATION: The proposed spectroscopic methods offer a rapid and nondestructive means for hazelnut value chain actors to verify the geographic origin of Giresun Tombul hazelnuts. This could definitely enhance consumer trust by ensuring product authenticity and potentially help in preventing fraud within the hazelnut market. In addition, these methods can also be used as a reference for future studies targeting the authentication of other shelled nuts.
Sujet(s)
Corylus , Noix , Analyse en composantes principales , Spectroscopie proche infrarouge , Analyse spectrale Raman , Corylus/composition chimique , Analyse spectrale Raman/méthodes , Spectroscopie proche infrarouge/méthodes , Analyse discriminante , Turquie , Noix/composition chimique , Machine à vecteur de support , Méthode des moindres carrés , Chimiométrie/méthodes , GéographieRÉSUMÉ
Element profiling is a powerful tool for detecting fraud related to claims of geographical origin. However, these methods must be continuously developed, as mixtures of different origins in particular offer great potential for adulteration. This study is a proof of principle to determine whether elemental profiling is suitable for detecting mixtures of the same food but from different origins and whether calculated data from walnut mixtures could help to reduce the measurement burden. The calculated data used in this study were generated based on measurements of authentic, unadulterated samples. Five different classification models and three regression models were applied in five different evaluation approaches to detect adulteration or even distinguish between adulteration levels (10% to 90%). To validate the method, 270 mixtures of walnuts from different origins were analyzed using inductively coupled plasma mass spectrometry (ICP-MS). Depending on the evaluation approach, different characteristics were observed in mixtures when comparing the calculated and measured data. Based on the measured data, it was possible to detect admixtures with an accuracy of 100%, even at low levels of adulteration (20%), depending on the country. However, calculated data can only contribute to the detection of adulterated walnut samples in exceptional cases.
Sujet(s)
Analyse d'aliment , Contamination des aliments , Juglans , Juglans/composition chimique , Contamination des aliments/analyse , Analyse d'aliment/méthodes , Spectrométrie de masse/méthodes , Noix/composition chimiqueRÉSUMÉ
Macadamia nuts are one of the most important economic food items in the world. Pericarp thickness and flavonoid composition are the key quality traits of Macadamia nuts, but the underlying mechanism of pericarp formation is still unknown. In this study, three varieties with significantly different pericarp thicknesses, namely, A38, Guire No.1, and HAES 900, at the same stage of maturity, were used for transcriptome analysis, and the results showed that there were significant differences in their gene expression profile. A total of 3837 new genes were discovered, of which 1532 were functionally annotated. The GO, COG, and KEGG analysis showed that the main categories in which there were significant differences were flavonoid biosynthesis, phenylpropanoid biosynthesis, and the cutin, suberine, and wax biosynthesis pathways. Furthermore, 63 MiMYB transcription factors were identified, and 56 R2R3-MYB transcription factors were clustered into different subgroups compared with those in Arabidopsis R2R3-MYB. Among them, the S4, S6, and S7 subgroups were involved in flavonoid biosynthesis and pericarp formation. A total of 14 MiMYBs' gene expression were verified by RT-qPCR analysis. These results provide fundamental knowledge of the pericarp formation regulatory mechanism in macadamia nuts.
Sujet(s)
Analyse de profil d'expression de gènes , Régulation de l'expression des gènes végétaux , Macadamia , Noix , Protéines végétales , Facteurs de transcription , Transcriptome , Macadamia/génétique , Macadamia/métabolisme , Protéines végétales/génétique , Protéines végétales/métabolisme , Analyse de profil d'expression de gènes/méthodes , Noix/génétique , Noix/métabolisme , Facteurs de transcription/génétique , Facteurs de transcription/métabolisme , Transcriptome/génétique , Flavonoïdes/biosynthèse , Flavonoïdes/métabolisme , Famille multigénique , Arabidopsis/génétique , Arabidopsis/métabolisme , PhylogenèseRÉSUMÉ
Almond trees are the most cultivated nut tree in the world. The production of almonds generates large amounts of by-products, much of which goes unused. Herein, this study aimed to develop a green chemistry approach to identify and extract potentially valuable compounds from almond by-products. Initially, a screening was performed with 10 different Natural Deep Eutectic Solvents (NADESs). The mixture lactic acid/glycerol, with a molar ratio 1:1 (1:50 plant material to NADES (w/v) with 20% v/v of water) was identified as the best extraction solvent for catechin, caffeoylquinic acid, and condensed tannins in almond hulls. Subsequently, a method was optimized by a Design of Experiment (DoE) protocol using a miniaturized extraction technique, Microwave-Assisted Extraction (MAE), in conjunction with the chosen NADESs. The optimal conditions were found to be 70 °C with 15 min irradiation time. The optimal extraction conditions determined by the DoE were confirmed experimentally and compared to methods already established in the literature. With these conditions, the extraction of metabolites was 2.4 times higher, according to the increase in total peak area, than the established literature methods used. Additionally, by applying the multiparameter Analytical Greenness Metric (AGREE) and Green Analytical Process Index (GAPI) metrics, it was possible to conclude that the developed method was greener than the established literature methods as it includes various principles of green analytical chemistry.
Sujet(s)
Extraits de plantes , Prunus dulcis , Prunus dulcis/composition chimique , Extraits de plantes/composition chimique , Extraits de plantes/isolement et purification , Micro-ondes , Technologie de la chimie verte/méthodes , Solvants/composition chimique , Biomimétique , Noix/composition chimiqueRÉSUMÉ
Nut-based products are a good source of high-quality plant protein in addition to mono- and polyunsaturated fatty acids, and may aid low-glycaemic dietary strategies important for the prevention of type 2 diabetes (T2D). In particular, they may be advantageous in populations susceptible to dysglycaemia, such as Asian Chinese. The present study aimed to compare effects of a higher-protein nut bar (HP-NB, also higher in total fibre and unsaturated fats, comprising mixed almonds and peanuts) vs. an isoenergetic higher-carbohydrate cereal bar (HC-CB) within the diet of 101 Chinese adults with overweight and normo- or hyperglycaemia. Ectopic pancreas and liver fat were characterised using magnetic resonance imaging and spectroscopy (MRI/S) as a secondary outcome. Participants were randomized to receive HP-NB or HC-CB daily as a 1 MJ light meal or snack replacement, in addition to healthy eating advice. Anthropometry and clinical indicators of T2D risk were assessed fasted and during an oral glucose tolerance test (OGTT), pre- and post-intervention. No significant difference was observed between diet groups for body weight, body mass index, waist or hip circumference, blood pressure, glucoregulatory markers, lipid profile or inflammatory markers over 12 weeks (all, p > 0.05). No difference was observed between glycaemic subgroups or those with normal versus high ectopic organ fat. Although HP-NB can attenuate postprandial glycaemia following a meal, no effects were observed for either fasting or glucose-mediated outcomes following longer-term inclusion in the habitual diet of Chinese adults with overweight, including at-risk subgroups.
Sujet(s)
Glycémie , Diabète de type 2 , Noix , Humains , Mâle , Femelle , Glycémie/métabolisme , Adulte d'âge moyen , Adulte , Diabète de type 2/diétothérapie , Diabète de type 2/prévention et contrôle , Hyperglycémie/prévention et contrôle , Chine , Asiatiques , Régime alimentaire/méthodes , Hyperglycémie provoquée , Surpoids/diétothérapie , Prunus dulcis , Arachis , Peuples d'Asie de l'EstRÉSUMÉ
BACKGROUND: Allergy to peanuts and tree nuts is a common cause of food allergy in Spain, with lipid transfer proteins (LTP) being the most frequently recognized panallergen. LTP sensitization often leads to multiple food group sensitivities, resulting in overly restrictive diets that hinder patient's quality of life. This study aimed to assess the tolerance of peanuts and tree nuts (hazelnuts and walnuts) in children sensitized to LTP, potentially mitigating the need for such diets. METHODS: This prospective study enrolled individuals diagnosed with allergy to peanuts, hazelnuts, or walnuts. Data were collected from medical records, including demographics and clinical history. Allergological assessment comprised skin prick tests using commercial extracts and the nuts in question, alongside measurements of total and specific IgE to nuts and their primary molecular components. Participants showing positive LTP sensitization without sensitization to seed storage proteins underwent open oral nut challenges. RESULTS: A total of 75 individuals labeled as allergic to peanuts, 44 to hazelnuts, and 51 to walnuts were included. All of them underwent an open oral provocation test with the incriminated nut, showing a high tolerance rate. Peanut was tolerated by 98.6% of patients, 97.72% tolerated hazelnut, and 84.3% tolerated walnut. CONCLUSION: The findings suggest that the majority of patients allergic to peanuts, hazelnuts, or walnuts, due to LTP sensitization and lacking IgE reactivity to seed storage proteins, can tolerate these nuts. This supports the need for personalized nut tolerance assessments to avoid unnecessary dietary restrictions.
Sujet(s)
Arachis , Protéines de transport , Tolérance immunitaire , Immunoglobuline E , Hypersensibilité aux noix , Tests cutanés , Humains , Mâle , Femelle , Protéines de transport/immunologie , Enfant , Espagne , Études prospectives , Enfant d'âge préscolaire , Immunoglobuline E/sang , Immunoglobuline E/immunologie , Hypersensibilité aux noix/immunologie , Hypersensibilité aux noix/diagnostic , Arachis/immunologie , Hypersensibilité aux arachides/immunologie , Hypersensibilité aux arachides/diagnostic , Allergènes/immunologie , Juglans/immunologie , Noix/immunologie , Adolescent , Corylus/immunologie , Hypersensibilité aux noix et aux arachides/immunologie , Antigènes végétaux/immunologieRÉSUMÉ
BACKGROUND: Regarding the importance of the prevention of non-communicable diseases (NCDs) and higher consumption of salt among the Iranian population than the level recommended by the World Health Organization, the aim of this study was to evaluate the accuracy of the salt mentioned in the traffic light labelling of nuts and seeds. Study Design: A cross-sectional study. METHODS: A total of 53 packaged nuts and seeds, including 7, 8, 9, 9, 10, and 10 samples of pumpkin, pistachios, almond, sunflower, peanut, and watermelon nuts and seeds, respectively, with traffic light labelling, were randomly purchased from several local markets in Isfahan, Iran. The amount of sodium was measured by the inductively coupled plasma-optical emission spectroscopy technique and then multiplied by 2.5 to achieve the amount of salt. RESULTS: Varying levels of traffic light labeling value accuracy were observed in most of the samples. In the almond, pistachio, peanut, and watermelon groups, the average amount of laboratory value had a statistically significant difference with the label value (P<0.05). CONCLUSION: The results demonstrated that the salt content of 82% of the studied samples had discrepancies with the values stated on the traffic light labelling. The presentation of an accurate amount of salt content is essential for promoting healthy eating habits and enabling individuals to make informed choices about their diet. It is recommended that regulatory authorities should review labelling guidelines and enforce stricter compliance to ensure accurate representation of salt content on packaged foods.
Sujet(s)
Étiquetage des aliments , Noix , Graines , Iran , Noix/composition chimique , Études transversales , Graines/composition chimique , Humains , Chlorure de sodium alimentaire/analyse , Prunus dulcis/composition chimique , Citrullus/composition chimique , Pistacia/composition chimiqueRÉSUMÉ
Tiger nut (TN) is a valuable nutrient and gluten-free tuber. To achieve high-quality TN flour as functional ingredients in food, it is essential to develop effective drying technologies for TN. Five drying methods including natural drying (Control), hot-air drying (HD), radio frequency single drying (RFSD), RF assisted hot-air drying (RFHD), and RF- vacuum drying (RFVD) were selected and compared to determine their effects on physiochemical, structural, and rheological properties of TN flour. Results showed that RF drying (RFD) significantly improved the hydration, oil-absorbing, and antioxidant activity capacity, especially for RFVD. RFHD exhibited greater color (BI = 13.80 ± 0.05 and C = 10.26 ± 0.05) and reducing sugar content (253.50 ± 2.27 mg d.b.) than RFSD and RFVD. The gelatinization temperature, enthalpy value, and particle size (57.30-269.33 µm) of TN flour were reduced. The structural property results indicated that RFD reduced the relative crystallinity and short-range ordering of the flour, altered protein secondary structure, and caused the damaged microstructure in comparison with Control and HD groups. All sample gels exhibited a weak strain overshoot behavior (type III) under large amplitude oscillations, and RFD resulted in a reduced viscoelastic behavior. RFD could be an effective method to produce functional TN flour.
Sujet(s)
Dessiccation , Farine , Rhéologie , Farine/analyse , Dessiccation/méthodes , Antioxydants/composition chimique , Phénomènes chimiques , Ondes hertziennes , Noix/composition chimique , ViscositéRÉSUMÉ
Walnut and hazelnut coallergy is a frequent manifestation in clinical practice whose molecular basis remains unclear. For this purpose, walnut-hazelnut cross-reactivity was evaluated in 20 patients allergic to one or both tree nuts and sensitized to their 2S albumins. Immunoblotting assays showed that 85% of patients recognized Jug r 1, walnut 2S albumin, which was associated with the development of severe symptoms; 50% of them corecognized hazelnut 2S albumin, Cor a 14. Both allergens were isolated using chromatographic techniques. Inhibition ELISAs revealed that Jug r 1 strongly inhibited the binding of Cor a 14-specific IgE, but Cor a 14 only partially inhibited Jug r 1-specific IgE binding. Our results showed that patients sensitized to walnut/hazelnut 2S albumins were not a homogeneous population. There were patients sensitized to specific epitopes of walnut 2S albumins and patients sensitized to cross-reactive epitopes between walnut and hazelnut, with Jug r 1 being the primary sensitizer.
Sujet(s)
Antigènes végétaux , Corylus , Réactions croisées , Immunoglobuline E , Juglans , Hypersensibilité aux noix , Noix , Juglans/composition chimique , Juglans/immunologie , Humains , Corylus/composition chimique , Corylus/immunologie , Femelle , Mâle , Hypersensibilité aux noix/immunologie , Adulte , Adulte d'âge moyen , Immunoglobuline E/immunologie , Noix/composition chimique , Noix/immunologie , Antigènes végétaux/immunologie , Antigènes végétaux/composition chimique , Albumines 2S de plante/immunologie , Albumines 2S de plante/composition chimique , Jeune adulte , Allergènes/immunologie , Allergènes/composition chimique , Adolescent , Protéines végétales/immunologie , Protéines végétales/composition chimique , Enfant , Sujet âgéRÉSUMÉ
OBJECTIVE: This study investigates the effects of a Brazil nut-enriched diet on body composition and bone parameters in CKD animal model. METHODS: Male Wistar rats were assigned to the following groups: Sham (n=8), Nx (n=6), nephrectomized rats, and NxBN (n=6), nephrectomized rats and an enricheddiet with 5% Brazil nut. Body composition parameters were obtained by dual-energy X- ray absorptiometry (DXA). Bioclin kits determined plasmatic calcium. The femurs werecollected to determine absolute mass and length, bone mineral density, and biomechanical tests. RESULTS: The NxBN group exhibited a higher total body bone mineral density (BMD) value than the Nx group (0.177±0.004g/cm2vs 0,169±0.003g/cm2; p=0.0397). No significant differences were observed regarding absolute mass, length, BMD, and biomechanical parameters in the femurs of the groups. Moreover, no significant differences were found in plasmatic calcium levels among the groups. CONCLUSIONS: Brazil-nut enriched diet modulated BMD in CKD experimental model, and further studies are demanded to understand the pathways involved in this finding.
Sujet(s)
Bertholletia , Composition corporelle , Densité osseuse , Régime alimentaire , Modèles animaux de maladie humaine , Fémur , Rat Wistar , Insuffisance rénale chronique , Animaux , Mâle , Insuffisance rénale chronique/diétothérapie , Insuffisance rénale chronique/physiopathologie , Rats , Régime alimentaire/méthodes , Fémur/physiopathologie , Absorptiométrie photonique , Calcium/sang , NoixRÉSUMÉ
This study aimed to develop and validate a multi-mycotoxin analysis method applied to cashew nuts by employing a miniaturized QuEChERS method followed by determination by ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Satisfactory recoveries for the concentrations 1, 10 and 30 ng g-1, ranging from 66% (fumonisin B1) to 110% (ochratoxin A) and relative standard deviations lower than 9% (fumonisin B2) were obtained for the target compounds. Limits of quantification ranged from 0.004 ng g-1 (sterigmatocystin) to 0.59 ng g-1 (alternariol). The applicability of the analytical method was verified by analyzing 30 cashew nut samples from the city of Rio de Janeiro, RJ, southeastern Brazil. Aflatoxins M1, G2, G1, B2, B1, ochratoxin A and sterigmatocystin were detected, respectively, in 27%, 10%, 17%, 30%, 30%, 30% and 50% of the analyzed samples, at maximum concentrations of 0.56, 0.67, 1.43, 2.02, 4.93, 4.81, and 0.35 ng g-1. The maximum limit established by Brazilian legislation for aflatoxins was not exceeded by any of the analyzed samples.
Sujet(s)
Anacardium , Contamination des aliments , Mycotoxines , Noix , Spectrométrie de masse en tandem , Mycotoxines/analyse , Anacardium/composition chimique , Chromatographie en phase liquide à haute performance , Contamination des aliments/analyse , Noix/composition chimique , Aflatoxines/analyse ,RÉSUMÉ
BACKGROUND: Areca nut polyphenols (AP) that extracted from areca nut, have been demonstrated for their potential of anti-fatigue effects. However, the underlying mechanisms for the anti-fatigue properties of AP has not been fully elucidated to date. Previous studies have predominantly concentrated on single aspects, such as antioxidation and anti-inflammation, yet have lacked comprehensive multi-dimensional analyses. PURPOSE: To explore the underlying mechanism of AP in exerting anti-fatigue effects. METHODS: In this study, we developed a chronic sleep deprivation-induced fatigue model and used physiological, hematological, and biochemical indicators to evaluate the anti- fatigue efficacy of AP. Additionally, a multi-omics approach was employed to reveal the anti-fatigue mechanisms of AP from the perspective of microbiome, metabolome, and proteome. RESULTS: The detection of physiology, hematology and biochemistry index indicated that AP markedly alleviate mice fatigue state induced by sleep deprivation. The 16S rRNA sequencing showed the AP promoted the abundance of probiotics (Odoribacter, Dubosiella, Marvinbryantia, and Eubacterium) and suppressed harmful bacteria (Ruminococcus). On the other hand, AP was found to regulate the expression of colonic proteins, such as increases of adenosine triphosphate (ATP) synthesis and mitochondrial function related proteins, including ATP5A1, ATP5O, ATP5L, ATP5H, NDUFA, NDUFB, NDUFS, and NDUFV. Serum metabolomic analysis revealed AP upregulated the levels of anti-fatigue amino acids, such as taurine, leucine, arginine, glutamine, lysine, and l-proline. Hepatic proteins express levels, especially tricarboxylic acid (TCA) cycle (CS, SDHB, MDH2, and DLST) and redox-related proteins (SOD1, SOD2, GPX4, and PRDX3), were significantly recovered by AP administration. Spearman correlation analysis uncovered the strong correlation between microbiome, metabolome and proteome, suggesting the anti-fatigue effects of AP is attribute to the energy homeostasis and redox balance through gut-liver axis. CONCLUSION: AP increased colonic ATP production and improve mitochondrial function by regulating gut microbiota, and further upregulated anti-fatigue amino acid levels in the blood. Based on the gut-liver axis, AP upregulated the hepatic tricarboxylic acid cycle and oxidoreductase-related protein expression, regulating energy homeostasis and redox balance, and ultimately exerting anti-fatigue effects. This study provides insights into the anti-fatigue mechanisms of AP, highlighting its potential as a therapeutic agent.
Sujet(s)
Areca , Fatigue , Polyphénols , Animaux , Souris , Areca/composition chimique , Modèles animaux de maladie humaine , Fatigue/traitement médicamenteux , Fatigue/étiologie , Microbiome gastro-intestinal/effets des médicaments et des substances chimiques , Multi-omique , Noix/composition chimique , Extraits de plantes/pharmacologie , Extraits de plantes/composition chimique , Polyphénols/pharmacologie , Privation de sommeil/complications , Privation de sommeil/traitement médicamenteuxRÉSUMÉ
This comprehensive study aimed to determine the level of nutritional compounds (20 amino acids, 11 phenolic acids, and 8 vitamins) and hazard compounds (14 mycotoxins) in ten types of conventional and ecological nuts from 25 countries. Moreover, chronic and acute toxicological risk assessment of mycotoxins was performed. Examined constituents were determined using LC-MS/MS. Ecological pine nuts showed the highest level of amino acids (233.87 g kg-1) compared to conventional (207 g kg-1), pecans-phenolic acids (816.6 mg kg-1 in ecological and 761 mg kg-1 in conventional), while pistachios-vitamins (3471.4 mg kg-1 in ecological and 3098.4 mg kg-1 in conventional). Increased concentration of mycotoxins was determined in conventional peanuts (54 µg kg-1) and walnuts (49.9 µg kg-1). Children were the most exposed population to acute intoxication with HT-2 toxin in conventional pistachios (20.66% ARfD). The results confirmed the nutritional importance of ecological nuts and emphasized the need for continuous screening of mycotoxins.
Sujet(s)
Contamination des aliments , Mycotoxines , Noix , Mycotoxines/analyse , Mycotoxines/composition chimique , Noix/composition chimique , Contamination des aliments/analyse , Appréciation des risques , Humains , Spectrométrie de masse en tandem , Acides aminés/analyse , Acides aminés/composition chimique , Vitamines/analyse , Vitamines/composition chimique , Valeur nutritiveRÉSUMÉ
"Horchata de chufa" is a beverage produced from tiger nut tubers, which yields a high amount of by-product. This study explored the functional properties of the Spanish tiger nut beverage (TNB) and its by-product (TNBP) together with the bioaccessibility and bioavailability of polyphenols in vitro. TNB and TNBP were characterized for polyphenols via LC/MS/MS and underwent in vitro digestion (INFOGEST). The total antioxidant capacity (TAC) of all bioaccessible fractions and digestion residues was assessed. Intestinal bioaccessible fractions were tested for the ability to inhibit the activity of digestive enzymes (α-amylase, α-glucosidase, and lipase) and the content of polyphenols, whose bioavailability was assessed in a Caco-2 cell model. Thirteen polyphenols were quantified and found to be more abundant in TNB (603 ± 1.4 µg g-1 DW) than in TNBP (187 ± 1.0 µg g-1 DW). Polyphenol bioaccessibility was higher for TNBP than that for TNB (57% vs. 27%), and despite a similar TAC of the intestinal bioaccessible fractions (10.2 ± 0.1 µmoL vs. 9.2 ± 0.03 µmoL eq. Trolox per g DW for TNB and TNBP, respectively), the different patterns of polyphenols released upon digestion suggested the higher ability of TNBP fraction to inhibit α-glucosidase and lipase. TNBP digestion residue showed higher TAC than TNB. Moreover, TNB polyphenols exhibited over 80% bioavailability, whereas TNBP polyphenols' bioavailability ranged from 62% to 84%. Overall, the findings demonstrated that TNBP maintains a high nutritional value, thus suggesting its possible reuse in innovative, healthy, and sustainable foods.
Sujet(s)
Biodisponibilité , Digestion , Polyphénols , Polyphénols/pharmacocinétique , Polyphénols/métabolisme , Humains , Cellules Caco-2 , Antioxydants/pharmacocinétique , Antioxydants/pharmacologie , Noix/composition chimique , Boissons/analyse , alpha-Glucosidase/métabolisme , Triacylglycerol lipase/métabolisme , Spectrométrie de masse en tandem , alpha-Amylases/métabolisme , alpha-Amylases/antagonistes et inhibiteurs , Extraits de plantes/composition chimique , Extraits de plantes/pharmacocinétique , Extraits de plantes/pharmacologieRÉSUMÉ
The aim of this study was to evaluate the effectiveness of nine different biological compounds to reduce mycotoxins concentrations. The hypothesis of this study was that a static in vitro gastrointestinal tract model, as an initial screening tool, can be used to simulate the efficacy of Geotrichum fermentans, Rhodotorula rubra, Kluyveromyce marxiamus yeast cell walls and their polysaccharides, red and white clay minerals, and walnuts nutshells claiming to detoxify AFB1, ZEA, DON, and T-2 toxin mycotoxins. Mycotoxin concentrations were analyzed using high-performance liquid chromatography (HPLC) with fluorescent (FLD) and ultraviolet detectors (UV). The greatest effects on reducing mycotoxin concentrations were determined as follows: for AFB1, inserted G. fermentans cell wall polysaccharides and walnut nutshells; for ZEA, inserted R. rubra and G. fermentans cell walls and red clay minerals; for DON, R. rubra cell wall polysaccharides and red clay minerals; and for T-2 toxin, R. rubra cell walls, K. marxianus, and G. fermentans cell wall polysaccharides and walnut nutshells. The present study indicated that selected mycotoxin-detoxifying biological compounds can be used to decrease mycotoxin concentrations.
Sujet(s)
Argile , Juglans , Mycotoxines , Rhodotorula , Juglans/composition chimique , Rhodotorula/métabolisme , Mycotoxines/analyse , Mycotoxines/composition chimique , Argile/composition chimique , Geotrichum/effets des médicaments et des substances chimiques , Geotrichum/métabolisme , Noix/composition chimique , Silicates d'aluminium/composition chimique , MinérauxRÉSUMÉ
Consumer preferences for walnut products are largely determined by the flavors released during mastication. In this study, a peeled walnut kernel (PWK) model was established with oral parameters decoupled using a Hutchings 3D model. The model explored in vitro variations using head-space solid-phase microextraction-gas chromatography-mass spectrometry and intelligent sensory techniques. The fracture strength, hardness, particle size, adhesiveness, springiness, gumminess, and chewiness were significantly reduced during mastication. We identified 61 volatile compounds and found that 2,5-dimethyl-3-ethylpyrazine is a key component, releasing predominantly baking and milky notes. Glutamic acid, alanine, arginine, and sucrose were identified as the key compounds in taste perception. The method can help establish a mastication model for nuts and facilitate breakthroughs in the development of walnut products and processing methods.
Sujet(s)
Chromatographie gazeuse-spectrométrie de masse , Juglans , Mastication , Noix , Goût , Composés organiques volatils , Juglans/composition chimique , Noix/composition chimique , Composés organiques volatils/analyse , Humains , Microextraction en phase solide , Dureté , Taille de particule , Aromatisants/analyseRÉSUMÉ
Interspecific American-European hazelnut (Corylus americana × C. avellana) hybrids are being developed for the Upper Midwest region of the United States, which have potential economic and ecological benefits. Currently, researchers are working on pre-commercialization initiatives to create a local hazelnut industry. However, a lack of knowledge on the sensory characteristics and consumer acceptability of the hybrid hazelnuts have been identified as potential limitations to production. This study investigated consumer responses to hybrid hazelnuts, employing hedonic ratings and check-all-that-apply (CATA) questions. Twelve roasted hazelnut hybrids were subjected to consumer evaluation for liking (aroma, color, flavor, texture, and overall) and aroma and flavor descriptors. Consumers also evaluated two European hazelnut varieties for comparative purposes. Liking scores were significantly different (p < 0.05) across different hybrid varieties and when comparing specific hybrids with European varieties. CATA sensory profiles specific to different varieties were also observed and discriminating attributes were identified using multiple-response methodologies. Penalty analysis based on CATA further identified the attributes roasty, hazelnut-like, caramel-like, chocolate-like, sweet, and salty as primary drivers of aroma/flavor liking, while earthy, burnt, and bitter were identified as primary drivers of disliking. Overall, unique flavors that impacted liking were found in hybrid hazelnuts and, when compared with European hazelnuts, provided insights for breeding and post-processing strategies to optimize product quality.