RÉSUMÉ
This study was carried out to investigate the effects of melatonin applications on postharvest quality changes of organic and conventionally grown plum fruit. Melatonin was applied in 0, 50, and 100 µmol L- 1 for organic and conventional samples. The fruits were stored at + 2.0 °C and 90% relative humidity for 28 days. During the storage period, the color, weight loss, firmness, Soluble solids concentration (SSC), titratable acidity (TA), pH, total antioxidant content, and total phenolics were evaluated at 7-day intervals. While no effect of melatonin applications on weight loss of organically grown plums was observed, it was determined that weight loss decreased as the dose of melatonin increased in conventionally grown plums. The lowest weight loss during storage was determined in conventionally grown plums treated with 100 µmolL- 1 melatonin. It was observed that the firmness values decreased as the storage period increased in both cultivation methods. The firmness decreased as the dose of melatonin application increased in organically grown plums, while the firmness increased as the dose of melatonin application increased in conventional cultivation. Melatonin application did not positively affect SSC, pH, and color values. However, it was determined that the mean TA values decreased as the dose of melatonin increased in both cultivation methods. When the total phenol content of organic and conventional plums was examined, it was determined that melatonin application decreased the number of phenolic compounds. The highest phenolic content was determined in the control samples. The total amount of antioxidants was 1.71 µmol TE g- 1 on the 28th day in the highest (100 µmol L- 1) melatonin-treated conventionally grown plums.
Sujet(s)
Stockage des aliments , Fruit , Mélatonine , Prunus domestica , Mélatonine/pharmacologie , Stockage des aliments/méthodes , Fruit/effets des médicaments et des substances chimiques , Fruit/croissance et développement , Fruit/composition chimique , Prunus domestica/effets des médicaments et des substances chimiques , Prunus domestica/croissance et développement , Antioxydants/métabolisme , Phénols/métabolisme , Agriculture biologique/méthodesRÉSUMÉ
Alcoholic fermentation is one of man's most efficient food preservation processes, and innovations in this area are a trend in food science and nutrition. In addition to the classic Saccharomyces yeasts, various other species may have desirable characteristics for obtaining fruit wines. This study investigated the profile of non-Saccharomyces commercial yeasts compared with S. cerevisiae regarding pineapple wine's chemical composition and bioaccessibility. The fermentation profile of the yeasts Lachancea thermotolerans, Brettanomyces bruxellensis, Brettanomyces lambicus, and S. cerevisiae was evaluated for sugar and alcohol content, and the pineapple wines obtained were analyzed for amino acids, phenolics, and organic acids by HPLC and volatile profile by GC/MS. All yeast strains were able to produce ethanol and glycerol at acceptable levels. L. thermotolerans produced higher levels of lactic acid (0.95 g/L) and higher consumption of free amino acids. B. bruxellensis produced higher levels of individual phenolics and ethanol 109 g/L. The alcoholic fermentation process improved the bioaccessibility of phenolics such as catechin (237 %), epigallocatechin gallate (81 %), procyanidin B1 (61 %) and procyanidin B2 (61 %). The yeasts differed in their volatile profiles, with Brettanomyces and Lachancea producing higher levels of compounds associated with pineapple aroma, such as ester ethyl butyrate (260-270 µg/L). These results demonstrate the importance of choosing the yeast strain for the conduction of alcoholic fermentation and that the yeasts Brettanomyces and Lachancea showed technological potential in obtaining pineapple wines. This study contributes to developing processes for obtaining fruit wines by highlighting two non-Saccharomyces yeast species with technological potential for alcoholic fermentations.
Sujet(s)
Ananas , Éthanol , Fermentation , Saccharomyces cerevisiae , Vin , Vin/analyse , Ananas/composition chimique , Saccharomyces cerevisiae/métabolisme , Éthanol/métabolisme , Éthanol/analyse , Phénols/analyse , Phénols/métabolisme , Acides aminés/analyse , Acides aminés/métabolisme , Brettanomyces/métabolisme , Saccharomycetales/métabolisme , Composés organiques volatils/analyse , Chromatographie gazeuse-spectrométrie de masse , Fruit/composition chimiqueRÉSUMÉ
The adsorbents used to remove taint compounds from wine can also remove constituents that impart desirable color, aroma, and flavor attributes, whereas molecularly imprinted polymers (MIPs) are tailor-made to selectively bind one or more target compounds. This study evaluated the potential for MIPs to ameliorate smoke taint in wine via removal of volatile phenols during or after fermentation. The addition of MIPs to smoke-tainted Pinot Noir wine (for 24 h with stirring) achieved 35-57% removal of guaiacol, 4-methylguaiacol, cresols, and phenol, but <10% of volatile phenol glycoconjugates were removed and some wine color loss occurred. Of the MIP treatments that were subsequently applied to Semillon and Merlot fermentations or wine, MIP addition post-inoculation of yeast yielded the best outcomes, both in terms of volatile phenol removal and wine sensory profiles. Despite some impact on other aroma volatiles and red wine color, the findings demonstrate that MIPs can ameliorate smoke-tainted wine.
Sujet(s)
Fermentation , Polymères à empreintes moléculaires , Odorisants , Fumée , Goût , Vin , Vin/analyse , Odorisants/analyse , Polymères à empreintes moléculaires/composition chimique , Humains , Composés organiques volatils/composition chimique , Composés organiques volatils/métabolisme , Phénols/composition chimique , Phénols/métabolisme , Mâle , Femelle , Adulte , Saccharomyces cerevisiae/métabolisme , Saccharomyces cerevisiae/composition chimique , Polymères/composition chimique , Polymères/métabolisme , AdsorptionRÉSUMÉ
Tyrosol is a natural phenolic compound with antioxidant, anti-inflammatory and other biological activities, serving as an important precursor of high-value products such as hydroxytyrosol and salidroside. Therefore, the green and efficient biosynthesis of tyrosol and its derivatives has become a research hotspot in recent years. Building cell factories by metabolic engineering of microorganisms is a potential industrial production way, which has low costs and environmental friendliness. This paper introduces the biosynthesis pathway of tyrosol and presents the key regulated nodes in the de novo synthesis of tyrosol in Escherichia coli and Saccharomyces cerevisiae. In addition, this paper reviews the recent advances in metabolic engineering for the production of hydroxytyrosol and salidroside. This review can provide a reference for engineering the strains for the high-yield production of tyrosol and its derivatives.
Sujet(s)
Escherichia coli , Génie métabolique , Alcool phénéthylique , Saccharomyces cerevisiae , Alcool phénéthylique/analogues et dérivés , Alcool phénéthylique/métabolisme , Génie métabolique/méthodes , Saccharomyces cerevisiae/métabolisme , Saccharomyces cerevisiae/génétique , Escherichia coli/métabolisme , Escherichia coli/génétique , Phénols/métabolisme , Glucosides/biosynthèse , Glucosides/métabolisme , Microbiologie industrielleRÉSUMÉ
Cyanobacteria possess special defense mechanisms to protect themselves against ultraviolet (UV) radiation. This study combines experimental and computational methods to identify the role of protective strategies in Nostoc species against UV-C radiation. To achieve this goal, various species of the genus Nostoc from diverse natural habitats in Iran were exposed to artificial UV-C radiation. The results indicated that UV-C treatment significantly reduced the photosynthetic pigments while simultaneously increasing the activity of antioxidant enzymes. Notably, N. sphaericum ISB97 and Nostoc sp. ISB99, the brown Nostoc species isolated from habitats with high solar radiations, exhibited greater resistance compared to the green-colored species. Additionally, an increase in scytonemin content occurred with a high expression of key genes associated with its synthesis (scyF and scyD) during the later stages of UV-C exposure in these species. The molecular docking of scytonemin with lipopolysaccharides of the cyanobacteria that mainly cover the extracellular matrix revealed the top/side positioning of scytonemin on the glycans of these lipopolysaccharides to form a UV-protective shield. These findings pave the way for exploring the molecular effects of scytonemin in forming the UV protection shield in cyanobacteria, an aspect that has been ambiguous until now.
Sujet(s)
Nostoc , Rayons ultraviolets , Nostoc/métabolisme , Nostoc/effets des radiations , Simulation de docking moléculaire , Phénols/métabolisme , Indoles/métabolisme , Indoles/composition chimique , Photosynthèse/effets des radiations , Lipopolysaccharides/métabolismeRÉSUMÉ
Although used in in vitro culture to boost secondary metabolite production, UV-B radiation can seriously affect plant growth if not properly dosed. Rosemary callus can be used as an important source of effective ingredients in the food and medicine industry. To balance the positive and negative effects of UV-B on rosmary callus, this study investigated the effects of melatonin on rosemary callus under UV-B radiation. The results showed that melatonin improved rosemary callus growth, with fresh weight and dry weight increased by 15.81% and 8.30%, respectively. The addition of 100 µM melatonin increased antioxidant enzyme activity and NO content in rosemary callus. At the same time, melatonin also significantly reduced membrane lipid damage and H2O2 accumulation in rosemary callus under UV-B stress, with malondialdehyde (MDA) and H2O2 contents reduced by 13.03% and 14.55%, respectively. In addition, melatonin increased the total phenol and rosmarinic acid contents in rosemary callus by 19% and 54%, respectively. Melatonin significantly improved the antioxidant activity of the extracts from rosemary callus. These results suggest that exogenous melatonin can alleviate the adverse effects of UV-B stress on rosemary callus by promoting NO accumulation while further enhancing phenolic accumulation and biological activity.
Sujet(s)
Antioxydants , Peroxyde d'hydrogène , Mélatonine , Phénols , Rosmarinus , Rayons ultraviolets , Mélatonine/pharmacologie , Mélatonine/métabolisme , Rosmarinus/métabolisme , Rosmarinus/effets des médicaments et des substances chimiques , Rosmarinus/effets des radiations , Antioxydants/métabolisme , Phénols/métabolisme , Peroxyde d'hydrogène/métabolisme , Malonaldéhyde/métabolisme , Stress physiologique/effets des radiations , Stress physiologique/effets des médicaments et des substances chimiques , , Cinnamates/métabolisme , Cinnamates/pharmacologie , Depsides/métabolismeRÉSUMÉ
BACKGROUND: Xanthenes and multi-aryl carbon core containing compounds represent different types of complex and condensed architectures that have impressive wide range of pharmacological, industrial and synthetic applications. Moreover, indoles as building blocks were only found in naturally occurring metabolites with di-aryl carbon cores and in chemically synthesized tri-aryl carbon core containing compounds. Up to date, rare xanthenes with indole bearing multicaryl carbon core have been reported in natural or synthetic products. The underlying mechanism of fluorescein-like arthrocolins with tetra-arylmethyl core were synthesized in an engineered Escherichia coli fed with toluquinol remained unclear. RESULTS: In this study, the Keio collection of single gene knockout strains of 3901 mutants of E. coli BW25113, together with 14 distinct E. coli strains, was applied to explore the origins of endogenous building blocks and the biogenesis for arthrocolin assemblage. Deficiency in bacterial respiratory and aromatic compound degradation genes ubiX, cydB, sucA and ssuE inhibited the mutant growth fed with toluquinol. Metabolomics of the cultures of 3897 mutants revealed that only disruption of tnaA involving in transforming tryptophan to indole, resulted in absence of arthrocolins. Further media optimization, thermal cell killing and cell free analysis indicated that a non-enzyme reaction was involved in the arthrocolin biosynthesis in E. coli. Evaluation of redox potentials and free radicals suggested that an oxygen-mediated free radical reaction was responsible for arthrocolins formation in E. coli. Regulation of oxygen combined with distinct phenol derivatives as inducer, 31 arylmethyl core containing metabolites including 13 new and 8 biological active, were isolated and characterized. Among them, novel arthrocolins with p-hydroxylbenzene ring from tyrosine were achieved through large scale of aerobic fermentation and elucidated x-ray diffraction analysis. Moreover, most of the known compounds in this study were for the first time synthesized in a microbe instead of chemical synthesis. Through feeding the rat with toluquinol after colonizing the intestines of rat with E. coli, arthrocolins also appeared in the rat blood. CONCLUSION: Our findings provide a mechanistic insight into in vivo synthesis of complex and condensed arthrocolins induced by simple phenols and exploits a quinol based method to generate endogenous aromatic building blocks, as well as a methylidene unit, for the bacteria-facilitated synthesis of multiarylmethanes.
Sujet(s)
Escherichia coli , Oxygène , Phénols , Escherichia coli/métabolisme , Escherichia coli/effets des médicaments et des substances chimiques , Escherichia coli/génétique , Phénols/métabolisme , Oxygène/métabolisme , Radicaux libres/métabolisme , Méthane/métabolisme , Animaux , Rats , Indoles/métabolismeRÉSUMÉ
Functional foods represent one of the fastest-growing, newer food category, and plant sources with functional properties are increasingly used as analogues of fermented milk-based derivatives. In this study, blended wort-rooibos beverages fermented with probiotic yeasts are proposed for the first time. Benefits of functional, non-conventional Lachancea thermotolerans (Lt101), Kazachstania unispora (Kum3-B3), Meyerozyma guilliermondii (Mg112), Meyerozyma caribbica (Mc58) and Debaryomyces hansenii (Dh36) yeast strains and the content of bioactive metabolites were evaluated. Viability tests on the probiotic yeasts confirmed previous results obtained in other matrices. The functional footprint of probiotic yeasts Lt101, Mg112 and Dh36 was confirmed by a balanced nutritional profile of the final drinks, also supported by aromatic and sensory analyses. In vitro estimated glycaemic index ranged between 77 % and 87 % without any influence on glycaemic response. Strains Dh36, Mc58, Kum3-B3 and Mg112 showed high antioxidant capacity and high total phenolic content, supporting the health promoting effect of the beverages.
Sujet(s)
Antioxydants , Fermentation , Aliments fermentés , Probiotiques , Levures , Levures/métabolisme , Aliments fermentés/microbiologie , Boissons/microbiologie , Aliment fonctionnel , Microbiologie alimentaire , Humains , Phénols/métabolisme , Phénols/analyseRÉSUMÉ
In this study, phenolic profile/content was analyzed by high-resolution untargeted metabolomics after short germination (72 h) and seedling growth (144 h), using three sorghum genotypes varying in tannin content (IS 29569, Macia and IS 30400). In vitro antioxidant capacity and phenolic bioaccessibility were determined by microplate-based and INFOGEST methods, respectively. A total of 58 % annotated compounds were found in all genotypes; and phenolic acids and flavonoids represent more than 80 % of sorghum total abundance. PCA analysis showed higher phenolic variability in germination times (72 %) than genotypes (51 %). Germination reduced total ion abundance (-7 %) and free:bound phenolic compounds ratio (2.4-1.1), but antioxidant capacity remained constant. These results indicate the cell matrix-phenolic decomplexation, with the free compounds were quickly consumed after radicle emergence. Germination increased phenolic bioaccessibility (mainly in oral phase) but reduces flavonoids contents in gastric/intestinal digestion steps. This work can stimulate seed germination as a viable option for sorghum-based foods development, with improved nutritional and bioactive properties.
Sujet(s)
Antioxydants , Germination , Phénols , Plant , Sorghum , Spectrométrie de masse en tandem , Sorghum/métabolisme , Sorghum/croissance et développement , Sorghum/composition chimique , Plant/croissance et développement , Plant/métabolisme , Phénols/métabolisme , Phénols/analyse , Chromatographie en phase liquide à haute performance , Spectrométrie de masse en tandem/méthodes , Antioxydants/métabolisme , Antioxydants/analyse , Flavonoïdes/analyse , Flavonoïdes/métabolisme , Graines/croissance et développement , Graines/métabolisme , Graines/composition chimique , Biodisponibilité , Métabolomique/méthodes , Génotype , Tanins/analyse , Tanins/métabolisme , DigestionRÉSUMÉ
The synthesis and differential allocation of reserve compounds is an important adaptive mechanism that enables species to resprout in fire-prone ecosystems. The analysis of compound allocation dynamics (differential accumulation of compounds between plant organs) provides insights into plant responses to disturbances. The aim was to quantify reserves in eight legume species from Cerrado open savannas with high fire frequency in order to investigate the patterns of allocation and distribution of compounds between leaves and underground organs, drawing ecophysiological inferences. The species were collected in 'campo sujo' areas of the Cerrado. Leaves and underground organs (xylopodium, taproot tubers) were subjected to physiological analyses. Overall, underground organs were characterised by greater deposits of carbohydrates, mainly soluble sugars, and also with the accumulation of proteins and amino acids. This suggests that nitrogen reserves, as well as carbohydrates, may have an ecophysiological function in response to fire, being allocated to the underground organs. Phenols were mainly evident in leaves, but a morphophysiological pattern was identified, where the two species with taproot tubers tended to concentrate more phenols in the underground portion compared to species with xylopodium, possibly due to functional differences between these organs. Such data allow inferring relevant ecophysiological dynamics in legumes from open savannas.
Sujet(s)
Fabaceae , Feuilles de plante , Fabaceae/métabolisme , Feuilles de plante/métabolisme , Incendies , Prairie , Brésil , Phénols/métabolisme , Racines de plante/métabolisme , Acides aminés/métabolisme , Tubercules/métabolismeRÉSUMÉ
Lipophenols, phenolic compounds esterified with fatty alcohols or fatty acids, provide greater health benefits upon dietary ingestion of plant-based foods than unesterified (poly)phenols. Based on this premise, the present study aimed to demonstrate the role of gastrointestinal enzymes (pepsin, pancreatin, and pancreatic lipase) in releasing alkyl gallates and trans-caffeates from wine lees, providing bioactive compounds with enhanced capacities against oxidative stress (OS) and para-inflammation. The UHPLC-ESI-QqQ-MS/MS-based analysis revealed ethyl gallate and ethyl trans-caffeate as the most prominent compounds (1.675 and 0.872 µg/g dw, respectively), while the bioaccessibility of the derivatives of gallic and caffeic acids was dependent on the alkyl chain properties. The de novo formation of alkyl gallates during gastric and intestinal digestion resulted from intestinal enzyme activity. Moreover, the in vitro capacity of bioaccessible alkyl esters of gallic and trans-caffeic acids to reduce cyclooxygenase-2 concentration and modulate oxilipins related to OS (8-iso-PGF2α) and inflammation (PGF2α and PGE2) was demonstrated in a time-dependent manner. In conclusion, the presence of alkyl esters of gallic and trans-caffeic acids in wine lees and their subsequent formation during digestion of this byproduct emphasize their value as a source of antioxidant and anti-inflammatory compounds, encouraging the consideration of wine lees as a valuable ingredient for health-promoting coproducts.
Sujet(s)
Cyclooxygenase 2 , Esters , Stress oxydatif , Phénols , Vin , Stress oxydatif/effets des médicaments et des substances chimiques , Vin/analyse , Phénols/composition chimique , Phénols/métabolisme , Esters/composition chimique , Esters/métabolisme , Cyclooxygenase 2/métabolisme , Humains , Inflammation/métabolisme , Vitis/composition chimique , Acides caféiques/composition chimique , Acides caféiques/métabolismeRÉSUMÉ
Hypericum perforatum transformed shoot lines (TSL) regenerated from corresponding hairy roots and non-transformed shoots (NTS) were comparatively evaluated for their phenolic compound contents and in vitro inhibitory capacity against target enzymes (monoamine oxidase-A, cholinesterases, tyrosinase, α-amylase, α-glucosidase, lipase, and cholesterol esterase). Molecular docking was conducted to assess the contribution of dominant phenolic compounds to the enzyme-inhibitory properties of TSL samples. The TSL extracts represent a rich source of chlorogenic acid, epicatechin and procyanidins, quercetin aglycone and glycosides, anthocyanins, naphthodianthrones, acyl-phloroglucinols, and xanthones. Concerning in vitro bioactivity assays, TSL displayed significantly higher acetylcholinesterase, tyrosinase, α-amylase, pancreatic lipase, and cholesterol esterase inhibitory properties compared to NTS, implying their neuroprotective, antidiabetic, and antiobesity potential. The docking data revealed that pseudohypericin, hyperforin, cadensin G, epicatechin, and chlorogenic acid are superior inhibitors of selected enzymes, exhibiting the lowest binding energy of ligand-receptor complexes. Present data indicate that H. perforatum transformed shoots might be recognized as an excellent biotechnological system for producing phenolic compounds with multiple health benefits.
Sujet(s)
Agrobacterium , Hypericum , Simulation de docking moléculaire , Phénols , Composés phytochimiques , Pousses de plante , Hypericum/composition chimique , Hypericum/métabolisme , Phénols/composition chimique , Phénols/pharmacologie , Phénols/métabolisme , Pousses de plante/composition chimique , Pousses de plante/métabolisme , Composés phytochimiques/composition chimique , Composés phytochimiques/pharmacologie , Extraits de plantes/composition chimique , Extraits de plantes/pharmacologie , Antienzymes/pharmacologie , Antienzymes/composition chimique , Végétaux génétiquement modifiés , alpha-Amylases/métabolisme , alpha-Amylases/antagonistes et inhibiteurs , alpha-Glucosidase/métabolisme , alpha-Glucosidase/composition chimiqueRÉSUMÉ
This research assesses the aboveground matter accumulation and Fv/Fm ratios (maximum quantum efficiency of PSII) in young plants (5months old) of Agave mapisaga and Agave salmiana grown under greenhouse conditions. This study also evaluated changes in the relative abundance of several different metabolites (sugars, free amino acids, and soluble phenols) during the major daily phases (I, III, and IV) of Crassulacean acid metabolism (CAM). These two species were also investigated to determine if differences in these parameters were evident with respect to their geographical origins (i.e. Metepec, Tlajomulco, and Tlaxiaca, in the state of Hidalgo, Mexico). Differences in shoot mass (0.51-0.82g plant-1 ), water content (75-93%), fructose (4-27µmolg-1 ), glucose (57-73µmolg-1 ), sucrose (10-30µmolg-1 ), free amino acids (5-25µmolg-1 ), soluble phenolics (0.7-3.5µmolg-1 ), and Fv/Fm ratios (0.75-0.80) were evident between plants with different origins. Specifically, at the end of Phase I compared to Phase IV, the results showed significant reductions in dry matter (up to 3.3%) and also reductions in fructose/sucrose. Relative amino acid concentrations were lowest in Phase III (8.8µmolg-1 ) compared to Phase I (16µmolg-1 ). These are novel observations, since all these changes and the biochemical and physiological performance in the CAM phases have not been previously determined in Agave plants differing in their geographical origins.
Sujet(s)
Agave , Acides aminés , Phénols , Agave/métabolisme , Agave/croissance et développement , Acides aminés/métabolisme , Phénols/métabolisme , Mexique , Pousses de plante/croissance et développementRÉSUMÉ
In the era dominated by plastic, the widespread use of plastic in our daily lives has led to a growing accumulation of its degraded byproducts, such as microplastics and plastic additives like Bisphenol A (BPA). BPA is recognized as one of the earliest man-made substances that exhibit endocrine-disrupting properties. It is frequently employed in the manufacturing of epoxy resins, polycarbonates, dental fillings, food storage containers, infant bottles, and water containers. BPA is linked to a range of health issues including obesity, diabetes, chronic respiratory illnesses, cardiovascular diseases, and reproductive abnormalities. This study examines the bacterial bioremediation of the BPA, which is found in many sources and is known for its hazardous effects on the environment. The metabolic pathways for the breakdown of BPA in important bacterial strains were hypothesized based on the observed altered intermediate metabolites during the degradation of BPA. This review discusses the enzymes and genes involved in the bacterial degradation of BPA. The utilization of naturally occurring microorganisms is the most efficient and cost-effective method due to their selectivity of strains, ensuring sustainability.
Sujet(s)
Bactéries , Composés benzhydryliques , Dépollution biologique de l'environnement , Phénols , Composés benzhydryliques/métabolisme , Phénols/métabolisme , Bactéries/métabolisme , Perturbateurs endocriniens/métabolisme , Polluants environnementaux/métabolismeRÉSUMÉ
Plant-derived ß-glucosidases hold promise for glycoside biosynthesis via reverse hydrolysis because of their excellent glucose tolerance and robust stability. However, their poor heterologous expression hinders the development of large-scale production and applications. In this study, we overexpressed apple seed ß-glucosidase (ASG II) in Komagataella phaffii and enhanced its production from 289 to 4322 U L-1 through expression cassette engineering and protein engineering. Upon scaling up to a 5-L high cell-density fermentation, the resultant mutant ASG IIV80A achieved a maximum protein concentration and activity in the secreted supernatant of 2.3 g L-1 and 41.4 kU L-1, respectively. The preparative biosynthesis of salidroside by ASG IIV80A exhibited a high space-time yield of 33.1 g L-1 d-1, which is so far the highest level by plant-derived ß-glucosidase. Our work addresses the long-standing challenge of the heterologous expression of plant-derived ß-glucosidase in microorganisms and presents new avenues for the efficient production of salidroside and other natural glycosides.
Sujet(s)
Glucosides , Malus , Phénols , Graines , bêta-Glucosidase , Phénols/métabolisme , bêta-Glucosidase/génétique , bêta-Glucosidase/métabolisme , Glucosides/biosynthèse , Glucosides/métabolisme , Glucosides/composition chimique , Graines/génétique , Graines/métabolisme , Saccharomycetales/génétique , Saccharomycetales/métabolisme , Saccharomycetales/enzymologie , Fermentation , Protéines végétales/génétique , Protéines végétales/métabolisme , Ingénierie des protéines/méthodesRÉSUMÉ
This study evaluated the potential of red pitaya pulp fermented with Lacticaseibacillus paracasei subsp. paracasei F-19 (F-19) as a base for probiotic products. Physicochemical parameters, sugar, betacyanin, and phenolic contents, and antioxidant activity were analyzed over 28 days at 4 °C and compared to a non-fermented pulp, and to a pulp fermented with Bifidobacterium animalis subsp. lactis BB-12 (BB-12). Volatile compounds were identified using HS-SPME/GC-MS. Probiotic viability during storage and survival through in vitro-simulated gastrointestinal tract (GIT) stress were assessed. Red pitaya pulp, rich in moisture (85.83 g/100 g), carbohydrates (11.65 g/100 g), and fibers (2.49 g/100 g), supported fermentation by both strains. F-19 and BB-12 lowered pH, with F-19 showing stronger acidification, and maintained high viability (8.85-8.90 log CFU/mL). Fermentation altered sugar profiles and produced unique volatile compounds, enhancing aroma and sensory attributes. F-19 generated 2-phenylethanol, a unique flavor compound, absent in BB-12. Phenolic content initially increased but antioxidant activity decreased during storage. Betacyanin remained stable for up to 14 days. Red pitaya improved F-19 viability through the simulated GIT, while BB-12 populations significantly decreased (p < 0.05). These results suggest red pitaya pulp is a promising plant-based matrix for F-19, offering protection during digestion and highlighting its potential as a functional food with enhanced bioactive compound bioavailability and sensory attributes.
Sujet(s)
Antioxydants , Bétacyanines , Cactaceae , Fermentation , Probiotiques , Composés organiques volatils , Bétacyanines/analyse , Composés organiques volatils/analyse , Composés organiques volatils/métabolisme , Antioxydants/analyse , Antioxydants/métabolisme , Cactaceae/composition chimique , Humains , Lacticaseibacillus paracasei/métabolisme , Phénols/analyse , Phénols/métabolisme , Goût , Bifidobacterium animalis/physiologie , Bifidobacterium animalis/métabolisme , Fruit/composition chimique , Aromatisants , Concentration en ions d'hydrogèneRÉSUMÉ
Rehmannia glutinosa produces many phenylethanoid glycoside (PhG) compounds, including salidroside, which not only possesses various biological activities but also is a core precursor of some medicinal PhGs, so it is very important to elucidate the species' salidroside biosynthesis pathway to enhance the production of salidroside and its derivations. Although some plant copper-containing amine oxidases (CuAOs), phenylacetaldehyde reductases (PARs) and UDP-glucose glucosyltransferases (UGTs) are thought to be vital catalytic enzymes involved in the downstream salidroside biosynthesis pathways, to date, none of these proteins or the associated genes in R. glutinosa have been characterized. To verify a postulated R. glutinosa salidroside biosynthetic pathway starting from tyrosine, this study identified and characterized a set of R. glutinosa genes encoding RgCuAO, RgPAR and RgUGT enzymes for salidroside biosynthesis. The functional activities of these proteins were tested in vitro by heterologous expression of these genes in Escherichia coli, confirming these catalytic abilities in these corresponding reaction steps of the biosynthetic pathway. Importantly, four enzyme-encoding genes (including the previously reported RgTyDC2 encoding tyrosine decarboxylase and the RgCuAO1, RgPAR1 and RgUGT2 genes) were cointegrated into Saccharomyces cerevisiae to reconstitute the R. glutinosa salidroside biosynthetic pathway, achieving an engineered strain that produced salidroside and validating these enzymes' catalytic functions. This study elucidates the complete R. glutinosa salidroside biosynthesis pathway from tyrosine metabolism in S. cerevisiae, establishing a basic platform for the efficient production of salidroside and its derivatives.
Sujet(s)
Voies de biosynthèse , Glucosides , Phénols , Rehmannia , Saccharomyces cerevisiae , Phénols/métabolisme , Saccharomyces cerevisiae/génétique , Saccharomyces cerevisiae/métabolisme , Glucosides/biosynthèse , Glucosides/métabolisme , Rehmannia/génétique , Rehmannia/métabolisme , Protéines végétales/génétique , Protéines végétales/métabolisme , Escherichia coli/génétique , Escherichia coli/métabolismeRÉSUMÉ
Microbial metabolism in landfill leachate systems is critically important in driving the degradation reactions of organic pollutants, including the emerging pollutant bisphenol A (BPA). However, little research has addressed the microbial degradation of BPA in landfill leachate and its interactions with nitrogen (N), sulfur (S), and methane (CH4) metabolism on a global scale. To this end, in this study on a global scale, an extremely high concentration of BPA was detected throughout the global landfill leachates. Subsequent reconstructive analyses of metagenomic datasets from 113 sites worldwide revealed that the predominant BPA-degrading microflora included Proteobacteria, Firmicutes, and Bacteroidota. Further metabolic analyses revealed that all four biochemical pathways involved in the degradation of BPA were achieved through biochemical cooperation between different bacterial members of the community. In addition, BPA degraders have also been found to actively collaborate synergistically with non-BPA degraders in the N and S removal as well as CH4 catabolism in landfill leachates. Collectively, this study not only provides insights into the dominant microbial communities and specific types of BPA-degrading microbial members in the community of landfill leachates worldwide, but also reveals the synergistic interactions between BPA mineralization and N, S, and CH4 metabolism. These findings offer valuable and important insights for future comprehensive and in-depth investigations into BPA metabolism in different environments.
Sujet(s)
Composés benzhydryliques , Dépollution biologique de l'environnement , Métagénomique , Méthane , Azote , Phénols , Soufre , Polluants chimiques de l'eau , Phénols/métabolisme , Polluants chimiques de l'eau/métabolisme , Composés benzhydryliques/métabolisme , Méthane/métabolisme , Soufre/métabolisme , Soufre/composition chimique , Azote/métabolisme , Installations d'élimination des déchets , Bactéries/métabolisme , Bactéries/génétique , Bactéries/classificationRÉSUMÉ
Nothofagus antarctica (G.Forst.) Oerst. (Ñire) leaves are a valuable source of (poly)phenolic compounds and represent a high-value non-timber product from Patagonian forests. However, information on the variability of their chemical profile is limited or non-existent. The aim of this study was to evaluate the (poly)phenolic variability in Ñire leaf infusions. To this end, different tree populations growing under different temperature regimes and soil characteristics were considered. Interestingly, a cup of Ñire leaf infusion could be considered as a rich source of quercetin. Significant differences in the (poly)phenolic content, especially in flavonoid conjugates and cinnamic acids, were found among the populations studied. These results suggest metabolic variability among the forests studied, which could be related to the species response to its growing conditions, and also provide some clues about the performance of N. antarctica under future climate scenarios. The N. antarctica forests growing in environments with lower frequency of cold and heat stress and high soil fertility showed better infusion quality. This study showed how a South American beech interacts with its local environment at the level of secondary metabolism. In addition, the information obtained is useful for defining forest management strategies in the Patagonian region.
Sujet(s)
Fagus , Feuilles de plante , Feuilles de plante/métabolisme , Feuilles de plante/composition chimique , Fagus/métabolisme , Fagus/croissance et développement , Sol/composition chimique , Forêts , Température , Phénols/analyse , Phénols/métabolisme , Flavonoïdes/analyse , Flavonoïdes/métabolismeRÉSUMÉ
Pseudomonas aeruginosa is an opportunistic pathogen that requires iron to survive in the host; however, the host immune system limits the availability of iron. Pyochelin (PCH) is a major siderophore produced by P. aeruginosa during infection, which can help P. aeruginosa survive in an iron-restricted environment and cause infection. The infection activity of P. aeruginosa is regulated by the Pseudomonas quinolone signal (PQS) quorum-sensing system. The system uses 2-heptyl-3-hydroxy-4-quinolone (PQS) or its precursor, 2-heptyl-4-quinolone (HHQ), as the signal molecule. PQS can control specific life processes such as mediating quorum sensing, cytotoxicity, and iron acquisition. This review summarizes the biosynthesis of PCH and PQS, the shared transport system of PCH and PQS, and the regulatory relationship between PCH and PQS. The correlation between the PQS and PCH is emphasized to provide a new direction for future research.