RÉSUMÉ
The nearly complete genome sequence of a novel polyomavirus from blood samples of Akodon montensis and Calomys tener collected in Brazil was determined by high-throughput sequencing. This virus showed a typical polyomaviruses genome organization, and it was classified as a member of the genus Betapolyomavirus. Our results expand the host range and viral diversity of the family Polyomaviridae.
Sujet(s)
Antigènes des virus oncogènes/génétique , Génome viral/génétique , Polyomaviridae , Sigmodontinae/virologie , Séquence d'acides aminés/génétique , Animaux , Brésil , Spécificité d'hôte , Phylogenèse , Polyomaviridae/classification , Polyomaviridae/génétique , Polyomaviridae/isolement et purificationRÉSUMÉ
A new real-time PCR assay for trichodysplasia spinulosa-associated polyomavirus (TSPyV) DNA detection was designed, and blood samples from kidney transplant recipients and healthy individuals were screened. TSPyV-DNA was not detected in blood from healthy individuals, but 26.8% of kidney recipients presented TSPyV-DNA. This is the first report of TSPyV viremia.
Sujet(s)
Sang/virologie , ADN viral/sang , Maladies du système pileux/virologie , Polyomaviridae/isolement et purification , Infections à polyomavirus/virologie , Réaction de polymérisation en chaine en temps réel , Adulte , Sujet âgé , Humains , Transplantation rénale/effets indésirables , Adulte d'âge moyen , Polyomaviridae/génétique , Receveurs de transplantation , Virémie/diagnosticRÉSUMÉ
Bats are the natural reservoir of a variety of viruses, including a polyomavirus (PyV) from a North American brown bat. We investigated 163 spleen samples from 22 bat species from French Guiana for the presence of PyVs. In total, we detected 25 PyV-positive animals belonging to nine different bat species. Phylogenetic analysis was performed on the genomes of eight representative PyVs, and showed that the bat PyVs form three distinct lineages within the genus Orthopolyomavirus and are genetically different from the previously described North American bat virus. Interestingly, two lineages cluster with PyVs found in chimpanzees, orangutans and gorillas. In addition, one group of bat PyVs is genetically related to the human Merkel cell polyomavirus.