RÉSUMÉ
MATERIALS AND METHODS: Locomotor outcomes in perforin-deficient (Pfp-/-) mice and wild-type littermate controls were measured after severe compression injury of the lower thoracic spinal cord up to six weeks after injury. RESULTS: According to both the Basso mouse scale score and single frame motion analysis, motor recovery of Pfp-/- mice was similar to wild-type controls. Interestingly, immunohistochemical analysis of cell types at six weeks after injury showed enhanced cholinergic reinnervation of spinal motor neurons caudal to the lesion site and neurofilament-positive structures at the injury site in Pfp-/- mice, whereas numbers of microglia/macrophages and astrocytes were decreased compared with controls. CONCLUSIONS: We conclude that, although, loss of perforin does not change the locomotor outcome after injury, it beneficially affects diverse cellular features, such as number of axons, cholinergic synapses, astrocytes and microglia/macrophages at or caudal to the lesion site. Perforin's ability to contribute to Rag2's influence on locomotion was observed in mice doubly deficient in perforin and Rag2 which recovered better than Rag2-/- or Pfp-/- mice, suggesting that natural killer cells can cooperate with T- and B-cells in spinal cord injury.
Sujet(s)
Locomotion/physiologie , Régénération nerveuse/physiologie , Perforines/physiologie , Traumatismes de la moelle épinière/enzymologie , Traumatismes de la moelle épinière/immunologie , Traumatismes de la moelle épinière/physiopathologie , Animaux , Comportement animal/physiologie , Modèles animaux de maladie humaine , Mâle , Souris , Souris de lignée C57BL , Perforines/déficitRÉSUMÉ
As a serious trauma of the neurological system, spinal cord injury (SCI) results in permanent disability, gives rise to immediate vascular damage and a wide range of matters that induce the breakage of blood spinal cord barrier (BSCB). SCI activates the expression of MMP-2/9, which are considered to accelerate the disruption of BSCB. Recent research shows that Dl-3-n-butylphthalide (NBP) exerted protective effects on blood spinal cord barrier in animals after SCI, but the underlying molecular mechanism of NBP on the BSCB undergoing SCI is unknown. Here, our research show that NBP inhibited the expression of MMP-2/9, then improved the permeability of BSCB following SCI. After the T9 level of spinal cord performed with a moderate injury, NBP was managed by intragastric administration and further performed once a day. NBP remarkably improved the permeability of BSCB and junction proteins degration, then promoted locomotion recovery. The protective effect of NBP on BSCB destruction is related to the regulation of MMP-2/9 induced by SCI. Moreover, NBP obviously inhibited the MMP-2/9 expression and junction proteins degradation in microvascular endothelial cells. In conclusion, our results indicate that MMP-2/9 are relevant to the breakdown of BSCB, NBP impairs BSCB destruction through inhibiting MMP-2/9 and promotes functional recovery subjected to SCI. NBP is likely to become a new nominee as a therapeutic to treat SCI via a transigent BSCB.
Sujet(s)
Benzofuranes/usage thérapeutique , Inhibiteurs de métalloprotéinases matricielles/usage thérapeutique , Neuroprotecteurs/usage thérapeutique , Traumatismes de la moelle épinière/traitement médicamenteux , Protéines de la jonction serrée/métabolisme , Jonctions serrées/effets des médicaments et des substances chimiques , Animaux , Hypoxie cellulaire/effets des médicaments et des substances chimiques , Claudine-5/métabolisme , Femelle , Glucose/déficit , Matrix metalloproteinase 2/métabolisme , Matrix metalloproteinase 9/métabolisme , Souris , Occludine/métabolisme , Oxygène/métabolisme , Rat Sprague-Dawley , Récupération fonctionnelle/effets des médicaments et des substances chimiques , Moelle spinale/effets des médicaments et des substances chimiques , Moelle spinale/métabolisme , Traumatismes de la moelle épinière/enzymologieRÉSUMÉ
Spinal cord injury (SCI) can cause chronic paralysis and incontinence and remains a major worldwide healthcare burden, with no regenerative treatment clinically available. Intraspinal transplantation of olfactory ensheathing cells (OECs) and injection of chondroitinase ABC (chABC) are both promising therapies but limited and unpredictable responses are seen, particularly in canine clinical trials. Sustained delivery of chABC presents a challenge due to its thermal instability; we hypothesised that transplantation of canine olfactory mucosal OECs genetically modified ex vivo by lentiviral transduction to express chABC (cOEC-chABC) would provide novel delivery of chABC and synergistic therapy. Rats were randomly divided into cOEC-chABC, cOEC, or vehicle transplanted groups and received transplant immediately after dorsal column crush corticospinal tract (CST) injury. Rehabilitation for forepaw reaching and blinded behavioural testing was conducted for 8 weeks. We show that cOEC-chABC transplanted animals recover greater forepaw reaching accuracy on Whishaw testing and more normal gait than cOEC transplanted or vehicle control rats. Increased CST axon sprouting cranial to the injury and serotonergic fibres caudal to the injury suggest a mechanism for recovery. We therefore demonstrate that cOECs can deliver sufficient chABC to drive modest functional improvement, and that this genetically engineered cellular and molecular approach is a feasible combination therapy for SCI.
Sujet(s)
Chondroitinases et chondroitin lyases/administration et posologie , Muqueuse olfactive/physiologie , Muqueuse olfactive/transplantation , Récupération fonctionnelle/physiologie , Traumatismes de la moelle épinière/enzymologie , Traumatismes de la moelle épinière/rééducation et réadaptation , Animaux , Cellules cultivées , Chondroitinases et chondroitin lyases/biosynthèse , Chiens , Mâle , Muqueuse olfactive/cytologie , Rats , Rat Wistar , Traumatismes de la moelle épinière/anatomopathologieRÉSUMÉ
BACKGROUND: The pathological process of traumatic spinal cord injury (SCI) involves excessive activation of microglia leading to the overproduction of proinflammatory cytokines and causing neuronal injury. Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine-1-phosphate (S1P), plays an important role in mediating inflammation, cell proliferation, survival, and immunity. METHODS: We aim to investigate the mechanism and pathway of the Sphk1-mediated neuroinflammatory response in a rodent model of SCI. Sixty Sprague-Dawley rats were randomly assigned to sham surgery, SCI, or PF543 (a specific Sphk1 inhibitor) groups. Functional outcomes included blinded hindlimb locomotor rating and inclined plane test. RESULTS: We discovered that Sphk1 is upregulated in injured spinal cord tissue of rats after SCI and is associated with production of S1P and subsequent NF-κB p65 activation. PF543 attenuated p65 activation, reduced inflammatory response, and relieved neuronal damage, leading to improved functional recovery. Western blot analysis confirmed that expression of S1P receptor 3 (S1PR3) and phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) are activated in microglia of SCI rats and mitigated by PF543. In vitro, we demonstrated that Bay11-7085 suppressed NF-κB p65 and inhibited amplification of the inflammation cascade by S1P, reducing the release of proinflammatory TNF-α. We further confirmed that phosphorylation of p38 MAPK and activation of NF-κB p65 is inhibited by PF543 and CAY10444. p38 MAPK phosphorylation and NF-κB p65 activation were enhanced by exogenous S1P and inhibited by the specific inhibitor SB204580, ultimately indicating that the S1P/S1PR3/p38 MAPK pathway contributes to the NF-κB p65 inflammatory response. CONCLUSION: Our results demonstrate a critical role of Sphk1 in the post-traumatic SCI inflammatory cascade and present the Sphk1/S1P/S1PR3 axis as a potential target for therapeutic intervention to control neuroinflammation, relieve neuronal damage, and improve functional outcomes in SCI.
Sujet(s)
Médiateurs de l'inflammation/métabolisme , Neurones/enzymologie , Phosphotransferases (Alcohol Group Acceptor)/métabolisme , Traumatismes de la moelle épinière/enzymologie , Animaux , Femelle , Méthanol/pharmacologie , Méthanol/usage thérapeutique , Souris , Neurones/anatomopathologie , Cellules PC12 , Pyrrolidines/pharmacologie , Pyrrolidines/usage thérapeutique , Rats , Rat Sprague-Dawley , Traumatismes de la moelle épinière/traitement médicamenteux , Traumatismes de la moelle épinière/anatomopathologie , Sulfones/pharmacologie , Sulfones/usage thérapeutique , Vertèbres thoraciques/traumatismesRÉSUMÉ
Traumatic spinal cord injury (SCI) enhances the activity of S-nitrosoglutathione reductase (GSNOR) and inhibits the mitochondrial aldehyde dehydrogenase 2 (ALDH2) activity, resulting in prolonged and sustained pain and functional deficits. This study's objective was to test the hypotheses that GSNOR's specific inhibitor N6022 mitigates pain and improves functional recovery in a mouse model of SCI. Furthermore, the degree of recovery is enhanced and the rate of recovery is accelerated by an ALDH2 activator Alda-1. Using both wild-type and GSNOR-/- mice, the SCI model deployed for groups was contusion at the T9-T10 vertebral level. The enzymatic activity of GSNOR and ALDH2 was measured, and the expression of GSNOR and ALDH2 was determined by western blot analysis. Functional improvements in experimental animals were assessed with locomotor, sensorimotor, and pain-like behavior tests. Wild-type SCI animals had enhanced GSNOR activity and decreased ALDH2 activity, leading to neurovascular dysfunction, edema, and worsened functional outcomes, including locomotor deficits and pain. Compared to wild-type SCI mice, GSNOR-/- mice had better functional outcomes. Monotherapy with either GSNOR inhibition by N6022 or enhanced ALDH2 activity by Alda-1 correlated well with functional recovery and lessened pain. However, combination therapy provided synergistic pain-relieving effects and more significant functional recovery compared with monotherapy. Conclusively, dysregulations in GSNOR and ALDH2 are among the causative mechanisms of SCI injury. Either inhibiting GSNOR or activating ALDH2 ameliorates SCI. Combining the specific inhibitor of GSNOR (N6022) with the selective activator of ALDH2 (Alda-1) provides greater protection to the neurovascular unit and confers greater functional recovery. The study is novel, and the combination therapy (N6022 + Alda-1) possesses translational potential.
Sujet(s)
Alcohol dehydrogenase/métabolisme , Aldehyde dehydrogenase, mitochondrial/métabolisme , Récupération fonctionnelle/effets des médicaments et des substances chimiques , Récupération fonctionnelle/physiologie , Traumatismes de la moelle épinière/enzymologie , Animaux , Benzamides/pharmacologie , Benzodioxoles/pharmacologie , Antienzymes/pharmacologie , Mâle , Souris , Souris de lignée C57BL , Souris knockout , Pyrroles/pharmacologieRÉSUMÉ
Long non-coding RNAs (lncRNAs) play important roles in various diseases, but the effect of lncRNA CASC9 on spinal cord injury (SCI) remains unclear. Therefore, the present study was conducted to explore the role of this lncRNA in SCI. SCI model was established by laminectomy in rats in vivo or induced by LPS in PC12 cells in vitro. Methylprednisolone (MP) was used for treatment in vivo. Spinal cord tissues were stained with H&E, and the oxidative stress- and inflammation-related factors were detected using their commercial kits. Cell apoptosis was determined using flow cytometry assay. Relative expression of corresponding genes was measured using qRT-PCR and western blotting. Luciferase reporter assay was used to verify binding site between CASC9 and miR-383-5p, as well as miR-383-5p and LDHA. The results showed that lncRNA CASC9 was downregulated and miR-383-5p was upregulated in SCI rats and LPS-induced PC12 cells. Severe histological injury and increased water content were also found in SCI rats. Increased levels of LDH, MDA, lactic acid, TNF-α, and IL-1ß were found in SCI rats and LPS-induced PC12 cells. These changes could be reversed by MP treatment in vivo or overexpression of CASC9 in vitro. Besides, overexpression of CASC9 decreased cell apoptosis and protein expression of LDHA and increased protein expression of Nrf2 and HO-1 in LPS-induced PC12 cells. Furthermore, miR-383-5p was a direct target of CASC9 and was negatively regulated by CASC9. LDHA was a direct target of miR-383-5p and was negatively regulated by CASC9. In conclusion, lncRNA CASC9 exerted a protective role against oxidative stress, inflammation, and cell apoptosis in SCI, providing a novel therapeutic target or prognostic factor for SCI.
Sujet(s)
Apoptose , Médiateurs de l'inflammation/métabolisme , L-Lactate dehydrogenase/métabolisme , microARN/métabolisme , Stress oxydatif , ARN long non codant/métabolisme , Traumatismes de la moelle épinière/enzymologie , Moelle spinale/enzymologie , Animaux , Anti-inflammatoires/pharmacologie , Apoptose/effets des médicaments et des substances chimiques , Cytokines/métabolisme , Modèles animaux de maladie humaine , Femelle , Régulation de l'expression des gènes , L-Lactate dehydrogenase/génétique , Laminectomie , Méthylprednisolone/pharmacologie , microARN/génétique , Facteur-2 apparenté à NF-E2/métabolisme , Cellules PC12 , ARN long non codant/génétique , Rats , Rat Sprague-Dawley , Transduction du signal , Moelle spinale/effets des médicaments et des substances chimiques , Moelle spinale/anatomopathologie , Traumatismes de la moelle épinière/traitement médicamenteux , Traumatismes de la moelle épinière/génétique , Traumatismes de la moelle épinière/anatomopathologieRÉSUMÉ
Axonal demyelination is a consistent pathological characteristic of Spinal cord injury (SCI). Promoting differentiation of oligodendrocytes is of importance for remyelination. Conversion of reactive astrocytes with stem cell potential to oligodendrocytes is proposed as an innovative strategy for SCI repair. Neuregulin-1 (Nrg1) plays an essential role in the differentiation of oligodendrocytes. Therefore, it's a potential treatment for demyelination in SCI that using Nrg1 to drive reactive astrocytes toward oligodendrocyte lineage cells. In this study, tumor necrosis factor-α (TNF-α) was used to induce dedifferentiation of primary rat spinal cord astrocytes into reactive astrocytes and Nrg1 was used to induce astrocytes in vitro and in vivo. The results showed that astrocytes treated with TNF-α expressed immaturity markers CD44 and Musashi1 at mRNA and protein levels, indicating that TNF-α induced the stem cell state of astrocytes. Nrg1 induced reactive astrocytes to express oligodendrocyte markers PDGFR-α and O4 at mRNA and protein levels, indicating that Nrg1 directly converts reactive astrocytes toward oligodendrocyte lineage cells. Moreover, upregulation of PI3K-AKT-mTOR signaling activation in response to Nrg1 was observed. In rats with SCI, intrathecal treatment with Nrg1 converted reactive astrocytes to oligodendrocyte lineage cells, inhibited astrogliosis, promoted remyelination, protected axons and eventually improved BBB score. All the biological effects of Nrg1 were significantly reversed by the co-administration of Nrg1 and ErbB inhibitor, suggesting that Nrg1 functioned through the receptor ErbB. Our findings indicate that Nrg1 is sufficient to trans-differentiate reactive astrocytes to oligodendrocytes via the PI3K-AKT-mTOR signaling pathway and repair SCI. Delivery of Nrg1 for the remyelination processes could be a promising strategy for spinal cord repair.
Sujet(s)
Astrocytes/effets des médicaments et des substances chimiques , Lignage cellulaire , Transdifférenciation cellulaire/effets des médicaments et des substances chimiques , Neuréguline-1/pharmacologie , Oligodendroglie/effets des médicaments et des substances chimiques , Phosphatidylinositol 3-kinase/métabolisme , Protéines proto-oncogènes c-akt/métabolisme , Traumatismes de la moelle épinière/traitement médicamenteux , Moelle spinale/effets des médicaments et des substances chimiques , Sérine-thréonine kinases TOR/métabolisme , Animaux , Astrocytes/enzymologie , Astrocytes/anatomopathologie , Cellules cultivées , Modèles animaux de maladie humaine , Récepteurs ErbB/métabolisme , Femelle , Gaine de myéline/métabolisme , Oligodendroglie/enzymologie , Oligodendroglie/anatomopathologie , Rat Sprague-Dawley , Transduction du signal , Moelle spinale/enzymologie , Moelle spinale/anatomopathologie , Traumatismes de la moelle épinière/enzymologie , Traumatismes de la moelle épinière/anatomopathologie , Facteur de nécrose tumorale alpha/pharmacologieRÉSUMÉ
The inhibition of glycogen synthase kinase-3 (GSK-3) can induce neurogenesis, and the associated activation of Wnt/ß-catenin signaling via GSK-3 inhibition may represent a means to promote motor function recovery following spinal cord injury (SCI) via increased astrocyte migration, reduced astrocyte apoptosis, and enhanced axonal growth. Herein, we assessed the effects of GSK-3 inhibition in vitro on the neurogenesis of ependymal stem/progenitor cells (epSPCs) resident in the mouse spinal cord and of human embryonic stem cell-derived neural progenitors (hESC-NPs) and human-induced pluripotent stem cell-derived neural progenitors (hiPSC-NPs) and in vivo on spinal cord tissue regeneration and motor activity after SCI. We report that the treatment of epSPCs and human pluripotent stem cell-derived neural progenitors (hPSC-NPs) with the GSK-3 inhibitor Ro3303544 activates ß-catenin signaling and increases the expression of the bIII-tubulin neuronal marker; furthermore, the differentiation of Ro3303544-treated cells prompted an increase in the number of terminally differentiated neurons. Administration of a water-soluble, bioavailable form of this GSK-3 inhibitor (Ro3303544-Cl) in a severe SCI mouse model revealed the increased expression of bIII-tubulin in the injury epicenter. Treatment with Ro3303544-Cl increased survival of mature neuron types from the propriospinal tract (vGlut1, Parv) and raphe tract (5-HT), protein kinase C gamma-positive neurons, and GABAergic interneurons (GAD65/67) above the injury epicenter. Moreover, we observed higher numbers of newly born BrdU/DCX-positive neurons in Ro3303544-Cl-treated animal tissues, a reduced area delimited by astrocyte scar borders, and improved motor function. Based on this study, we believe that treating animals with epSPCs or hPSC-NPs in combination with Ro3303544-Cl deserves further investigation towards the development of a possible therapeutic strategy for SCI.
Sujet(s)
Glycogen Synthase Kinase 3/antagonistes et inhibiteurs , Cellules souches multipotentes/effets des médicaments et des substances chimiques , Neurogenèse/effets des médicaments et des substances chimiques , Traumatismes de la moelle épinière/traitement médicamenteux , Animaux , Technique de Western , Modèles animaux de maladie humaine , Femelle , Humains , Souris , Souris de lignée C57BL , Traumatismes de la moelle épinière/enzymologie , Transplantation de cellules souchesRÉSUMÉ
Enolase inhibition is a potential therapeutic strategy currently being investigated for treatment of spinal cord injury (SCI) as it reduces pro-inflammatory cytokines and chemokines, alters metabolic factors, and reduces gliosis in acute SCI. Herein, the role of enolase in SCI has been examined to better understand the effects of this enzyme on inflammation, metabolic hormones, glial cell activation, and neuroprotection under these shorter injury conditions. Immunohistochemical analyses of inflammatory markers vimentin, Cox-2, and caspase-1 indicated that enolase inhibition attenuated the elevated levels of inflammation seen following SCI. Iba1, GFAP, NFP, and CSPG staining indicated that enolase inhibition with prolonged administration of ENOblock reduced microglia/astrocyte activation and lead to enhanced neuroprotection in SCI. An analysis of metabolic hormones revealed that ENOblock treatment significantly upregulated plasma concentrations of peptide YY, glucagon-like peptide 1, glucose-dependent insulinotropic peptide, glucagon, and insulin hormones as compared to vehicle-treated controls (Mann-Whitney, p ≤ 0.05). ENOblock did not have a significant effect on plasma concentrations of pancreatic polypeptide. Interestingly, ENOblock treatment inhibited chondroitin sulfate proteoglycan (CSPG), which is produced by activated glia and serves to block regrowth of axons across the lesion site following injury. An increased level of NeuN and MBP with reduced caspase-1 was detected in SCI tissues after ENOblock treatment, suggesting preservation of myelin and induction of neuroprotection. ENOblock also induced improved motor function in SCI rats, indicating a role for enolase in modulating inflammatory and metabolic factors in SCI with important implications for clinical consideration.
Sujet(s)
Benzamides/usage thérapeutique , Médiateurs de l'inflammation/antagonistes et inhibiteurs , Neuroprotection/effets des médicaments et des substances chimiques , Hormones peptidiques/agonistes , Enolase/antagonistes et inhibiteurs , Traumatismes de la moelle épinière/traitement médicamenteux , Triazines/usage thérapeutique , Animaux , Benzamides/pharmacologie , Hormones , Médiateurs de l'inflammation/métabolisme , Mâle , Neuroprotection/physiologie , Hormones peptidiques/métabolisme , Enolase/métabolisme , Rats , Rat Sprague-Dawley , Récupération fonctionnelle/effets des médicaments et des substances chimiques , Récupération fonctionnelle/physiologie , Traumatismes de la moelle épinière/enzymologie , Vertèbres thoraciques/traumatismes , Triazines/pharmacologieRÉSUMÉ
The objective of this study was to explore the neuroprotective molecular mechanisms of erythropoietin (EPO) in rats following spinal cord injury (SCI). First, a standard SCI model was established. After drug or saline treatment was administered, locomotor function was evaluated in rats using the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. H&E, Nissl, and TUNEL staining were performed to assess the ratio of cavities, number of motor neurons, and apoptotic cells in the damaged area. The relative protein and mRNA expressions were examined using western blot and qRT-PCR analyses, and the inflammatory markers, axon special protein, and neuromuscular junctions (NMJs) were detected by immunofluorescence. Both doses of EPO notably improved locomotor function, but high-dose EPO was more effective than low-dose EPO. Moreover, EPO reduced the cavity ratio, cell apoptosis, and motor neuron loss in the damaged area, but enhanced the autophagy level and extracellular-regulated protein kinase (ERK) activity. Treatment with an ERK inhibitor significantly prevented the effect of EPO on SCI, and an activator mimicked the benefits of EPO. Further investigation revealed that EPO promoted SCI-induced autophagy via the ERK signaling pathway. EPO activates autophagy to promote locomotor function recovery in rats with SCI via the ERK signaling pathway.
Sujet(s)
Autophagie , Érythropoïétine/usage thérapeutique , Système de signalisation des MAP kinases , Neuroprotecteurs/usage thérapeutique , Traumatismes de la moelle épinière/traitement médicamenteux , Traumatismes de la moelle épinière/physiopathologie , Animaux , Anti-inflammatoires/pharmacologie , Anti-inflammatoires/usage thérapeutique , Apoptose/effets des médicaments et des substances chimiques , Survie cellulaire/effets des médicaments et des substances chimiques , Régulation négative/effets des médicaments et des substances chimiques , Érythropoïétine/pharmacologie , Inflammation/complications , Inflammation/anatomopathologie , Système de signalisation des MAP kinases/effets des médicaments et des substances chimiques , Motoneurones/effets des médicaments et des substances chimiques , Motoneurones/métabolisme , Motoneurones/anatomopathologie , Neuroprotecteurs/pharmacologie , Inhibiteurs de protéines kinases/pharmacologie , Inhibiteurs de protéines kinases/usage thérapeutique , Rat Sprague-Dawley , Récupération fonctionnelle/effets des médicaments et des substances chimiques , Moelle spinale/effets des médicaments et des substances chimiques , Moelle spinale/anatomopathologie , Moelle spinale/physiopathologie , Traumatismes de la moelle épinière/enzymologie , Traumatismes de la moelle épinière/prévention et contrôle , Sérine-thréonine kinases TOR/métabolisme , Régulation positive/effets des médicaments et des substances chimiquesRÉSUMÉ
We investigate the anti-inflammatory effects of injectable hydrogel containing tauroursodeoxycholic acid (TUDCA) in a spinal cord injury (SCI) model. To this end, TUDCA-hydrogel (TC gel) is created by immersing the synthesized hydrogel in a TUDCA solution for 1 h. A mechanical SCI was imposed on rats, after which we injected the TC gel. After the SCI and injections, motor functions and lesions were significantly improved in the TC gel group compared with those in the saline group. The TC gel significantly decreased pro-inflammatory cytokine levels compared with the saline; TUDCA and glycol chitosan-oxidized hyaluronate were mixed at a ratio of 9:1 (CHA) gel independently. In addition, the TC gel significantly suppressed the phosphorylation of extracellular signal-regulated kinase (p-ERK) and c-Jun N-terminal kinase (p-JNK) in the mitogen-activated protein kinase (MAPK) pathway compared with the saline, TUDCA, and CHA gel independently. It also decreased tumor necrosis factor-α (TNF-α) and glial fibrillary acidic protein (GFAP), inflammatory marker, at the injured sites more than those in the saline, TUDCA, and CHA gel groups. In conclusion, the results of this study demonstrate the neuroinflammatory inhibition effects of TC gel in SCI and suggest that TC gel can be an alternative drug system for SCI cases.
Sujet(s)
Anti-inflammatoires/usage thérapeutique , Hydrogels/composition chimique , Injections , Traumatismes de la moelle épinière/traitement médicamenteux , Acide taurochénodésoxycholique/usage thérapeutique , Animaux , Anti-inflammatoires/pharmacologie , Apoptose/effets des médicaments et des substances chimiques , Comportement animal , Chitosane/composition chimique , Cytokines/métabolisme , Protéine gliofibrillaire acide/métabolisme , Acide hyaluronique/composition chimique , Médiateurs de l'inflammation/métabolisme , Système de signalisation des MAP kinases/effets des médicaments et des substances chimiques , Activité motrice/effets des médicaments et des substances chimiques , Sialidase/métabolisme , Phosphorylation/effets des médicaments et des substances chimiques , Rat Sprague-Dawley , Récupération fonctionnelle/effets des médicaments et des substances chimiques , Traumatismes de la moelle épinière/enzymologie , Traumatismes de la moelle épinière/physiopathologie , Acide taurochénodésoxycholique/pharmacologie , Facteur de nécrose tumorale alpha/métabolismeRÉSUMÉ
Spinal cord injury (SCI) induces a series of endogenous biochemical changes that lead to secondary degeneration, including apoptosis. The aim of this study was to investigate the potential effect and mechanism of action of MGMT in strengthing neuronal apoptosis following SCI. To determine MGMT-mediated apoptosis in spinal cord injury, we performed western blot and analyzed the expression change of MGMT with different timepoints. Western blot analysis showed the upregulation of MGMT has a peak at 21 days in injured spinal cord tissues. Expression and location was observed in the neurons after SCI. Upregulation of p53, Bax, cleaved caspase3 and cleaved caspase9 and downregulation of Bcl2 were detected after SCI. Co-localization of cleaved caspase3 with MGMT indicated MGMT involved in apoptosis taking place after SCI. In addition, we carried out H2O2 stimulation to further confirm MGMT played a role in neuron apoptosis process and activated p53 signaling pathway in vitro. Finally, based above data, we packaged lenti-associated virus inhibit MGMT expression and injected into rat spinal cords after SCI model was built. LV-MGMT not only reduces the neuron apoptosis, but also increases GAP43 expression and promotes hindlimbs locomotor function recovery. Taken together, the in vivo data and the in vitro observations prove MGMT-mediated apoptosis in the injured spinal cord.
Sujet(s)
Apoptose , DNA modification methylases/métabolisme , Enzymes de réparation de l'ADN/métabolisme , Neurones/enzymologie , Neurones/anatomopathologie , Traumatismes de la moelle épinière/enzymologie , Traumatismes de la moelle épinière/anatomopathologie , Protéines suppresseurs de tumeurs/métabolisme , Animaux , Techniques de knock-down de gènes , Peroxyde d'hydrogène , Injections , Lentivirus/métabolisme , Mâle , Cellules PC12 , Rats , Rat Sprague-Dawley , Récupération fonctionnelle , Moelle spinale/anatomopathologie , Traumatismes de la moelle épinière/physiopathologieRÉSUMÉ
Spinal cord injury (SCI) is a neurological disease commonly caused by traumatic events on spinal cords. MiRNA-92a-3p is reported to be down-regulated after SCI. Our study investigated the effects of up-regulated miR-92a-3p on SCI and the underlying mechanisms. SCI mice model was established to evaluate the functional recovery of hindlimbs of mice through open-field locomotion and scored by Basso, Beattie, and Bresnahan (BBB) locomotion scale. Apoptosis of spinal cord cells was determined by flow cytometry. The effects of miR-92a-3p on SCI were detected by intrathecally injecting miR-92a-3p agomiR (agomiR-92) into the mice prior to the establishment of SCI. Phosphatase and tensin homolog (PTEN) was predicted as a target of miR-29a-3p by TargetScan. We further assessed the effects of agomiR-92 or/and overexpressed PTEN on apoptosis rates and apoptotic protein expressions in SCI mice. Moreover, the activation of protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling was determined by Western blot. The results showed that compared with the sham-operated mice, SCI mice had much lower BBB scores, and theapoptosis rate of spinal cord cells was significantly increased. After SCI, the expression of miR-92a-3p was down-regulated, and increased expression of miR-92a-3p induced by agomiR-92 further significantly increased the BBB score and decreased apoptosis. PTEN was specifically targeted by miR-92a-3p. In addition, the phosphorylation levels of Akt and mTOR were up-regulated under the treatment of agomiR-92. Our data demonstrated that the neuroprotective effects of miR-92a-3p on spinal cord safter SCI were highly associated with the activation of the PTEN/AKT/mTOR pathway.
Sujet(s)
Apoptose , Membre pelvien/innervation , microARN/métabolisme , Phosphohydrolase PTEN/métabolisme , Traumatismes de la moelle épinière/enzymologie , Moelle spinale/enzymologie , Animaux , Modèles animaux de maladie humaine , Femelle , Régulation de l'expression des gènes , Locomotion , Souris de lignée C57BL , microARN/génétique , Phosphohydrolase PTEN/génétique , Protéines proto-oncogènes c-akt/métabolisme , Récupération fonctionnelle , Transduction du signal , Moelle spinale/anatomopathologie , Moelle spinale/physiopathologie , Traumatismes de la moelle épinière/génétique , Traumatismes de la moelle épinière/anatomopathologie , Traumatismes de la moelle épinière/physiopathologie , Sérine-thréonine kinases TOR/métabolismeRÉSUMÉ
Spinal cord injury (SCI) is a devastating disease. Strategies that enhance the intrinsic regenerative ability are very important for the recovery of SCI to radically prevent the occurrence of sensory disorders. Epidermal growth factor (EGF) showed a limited effect on the growth of primary sensory neuron neurites due to the degradation of phosphorylated-epidermal growth factor receptor (p-EGFR) in a manner dependent on Casitas B-lineage lymphoma (CBL) (an E3 ubiquitin-protein ligase). MiR-22-3p predicted from four databases could target CBL to inhibit the expression of CBL, increase p-EGFR levels and neurites length via STAT3/GAP43 pathway rather than Erk1/2 axis. EGF, EGFR, and miR-22-3p were downregulated sharply after injury. In vivo miR-22-3p Agomir application could regulate CBL/p-EGFR/p-STAT3/GAP43/p-GAP43 axis, and restore spinal cord sensory conductive function. This study clarified the mechanism of the limited promotion effect of EGF on adult primary sensory neuron neurite and targeting miR-22-3p could be a novel strategy to treat sensory dysfunction after SCI.
Sujet(s)
Protéines adaptatrices de la transduction du signal/métabolisme , Récepteurs ErbB/métabolisme , Protéine GAP-43/métabolisme , microARN/métabolisme , Régénération nerveuse , Protéines proto-oncogènes c-cbl/métabolisme , Facteur de transcription STAT-3/métabolisme , Cellules réceptrices sensorielles/enzymologie , Traumatismes de la moelle épinière/enzymologie , Protéines adaptatrices de la transduction du signal/génétique , Animaux , Cellules cultivées , Modèles animaux de maladie humaine , Facteur de croissance épidermique/pharmacologie , Récepteurs ErbB/agonistes , Potentiels évoqués somatosensoriels , Femelle , microARN/génétique , Régénération nerveuse/effets des médicaments et des substances chimiques , Excroissance neuronale , Oligonucléotides/pharmacologie , Phosphorylation , Culture de cellules primaires , Protéines proto-oncogènes c-cbl/génétique , Rat Wistar , Récupération fonctionnelle , Cellules réceptrices sensorielles/effets des médicaments et des substances chimiques , Cellules réceptrices sensorielles/anatomopathologie , Transduction du signal , Traumatismes de la moelle épinière/traitement médicamenteux , Traumatismes de la moelle épinière/anatomopathologie , Traumatismes de la moelle épinière/physiopathologieRÉSUMÉ
OBJECTIVE: This study aims to investigate the potential function of miR-325-3p in vascular integrity and inflammatory response following spinal cord injury (SCI). MATERIALS AND METHODS: The protein levels of ANG-1, ANG-2, and caspase-3 in HUVECs incubated with 0, 100, 200, 400, and 800 ng/ml NE for 24 h were determined. The regulatory effect of overexpressed miR-325-3p on the protein levels of ANG-1 and ANG-2 was determined by Western blot. The SCI model in SD rats was established by spinal injury at T10. Subsequently, the relative levels of miR-325-3p, ANG-1, and ANG-2 were determined in SCI rats and controls. Furthermore, SCI rats were administrated with miR-325-3p mimics or negative control and the relative levels of miR-325-3p, ANG-1, and ANG-2 were examined as well. At day 14, the protein levels of iNOS and GFAP in SCI rats and those overexpressing miR-325-3p were detected. BBB (Basso, Beattie, and Bresnahan) locomotor rating scale was applied for evaluating the locomotor function recovery at day 1, 3, 7, 14, 21, and 28 following SCI. RESULTS: NE treatment in HUVECs downregulated ANG-1 and upregulated ANG-2 and caspase-3 in a dose-dependent manner. The overexpression of miR-325-3p upregulated NE-induced decreased the level of ANG-1 and downregulated NE-induced increased level of ANG-2. After the establishment of the SCI model in rats, the miR-325-3p level gradually decreased in SCI rats relative to controls in a time-dependent manner. ANG-1 level in SCI rats decreased to the lowest on the first day following SCI, and gradually increased at day 3, 5, and 7. ANG-2 level was firstly upregulated and achieved the peak on day 3, and then decreased at day 5 and 7. Moreover, SCI rats overexpressing miR-325-3p showed a higher level of ANG-1 and lower level of ANG-2 than those of SCI rats. Overexpression of miR-325-3p downregulated the protein levels of iNOS and GFAP in SCI rats. BBB scale showed elevated locomotor function recovery in SCI rats overexpressing miR-325-3p compared with SCI rats. CONCLUSIONS: MiR-325-3p protects the integrity of the vascular wall, reduces infiltration of inflammation, and improves locomotor function recovery at post-SCI.
Sujet(s)
Expression des gènes , Leukocyte elastase/antagonistes et inhibiteurs , microARN/génétique , Activité motrice/génétique , Traumatismes de la moelle épinière/enzymologie , Angiopoïétine-1/génétique , Angiopoïétine-1/métabolisme , Angiopoïétine-2/génétique , Angiopoïétine-2/métabolisme , Animaux , Apoptose/effets des médicaments et des substances chimiques , Apoptose/génétique , Modèles animaux de maladie humaine , Relation dose-effet des médicaments , Endothélium vasculaire/effets des médicaments et des substances chimiques , Endothélium vasculaire/métabolisme , Expression des gènes/effets des médicaments et des substances chimiques , Cellules endothéliales de la veine ombilicale humaine , Humains , Leukocyte elastase/génétique , Leukocyte elastase/pharmacologie , Mâle , Rat Sprague-Dawley , Traumatismes de la moelle épinière/génétiqueRÉSUMÉ
Spinal cord injury (SCI) is terrible damage leading to the deficiencies and results in infinite inconvenience to sufferers. The effective treatment for SCI still meets a larger number of problems. Herein, the underlying molecular mechanism and novel therapy of SCI are urgently to investigate. Arachidonate 12-lipoxygenase (ALOX12) is widely expressed in various cell types and plays important role in modulating different cellular processes, such as platelet aggregation, cell migration and cancer cell proliferation. Nevertheless, the effects of ALOX12 on SCI are unclear. In the study, SCI model was established in wild type (WT) mice and ALOX12 knockout mice. First, ALOX12 expression was up-regulated in spinal cord tissues of WT mice after SCI. ALOX12-knockout mice exhibited improved behavior after SCI operation. Glial activation triggered by SCI was also alleviated in mice with the loss of ALOX12, as evidenced by the down-regulated expression of glial fibrillary acidic protein (GFAP) and Iba-1 in spinal cord samples. Further, SCI-induced inflammation was markedly prevented in ALOX12-knockout mice through blocking inhibitor of NF-κB α (IκBα)/nuclear factor-κB (NF-κB) pathway signaling. Additionally, reducing ALOX12 expression attenuated apoptosis in spinal cord tissues of SCI mice by decreasing Cyto-c, cleaved Caspase-3 and poly (ADP-ribose) polymerases (PARP) expression. The protective role of ALOX12-decrease against SCI was verified in LPS-incubated glial cells through repressing inflammatory response and apoptotic formation. Moreover, transgenic mice with ALOX12 over-expression showed accelerated SCI, associated with intensified inflammation and apoptosis. Based on these results, strategies for inhibiting ALOX12 could be used to prevent SCI development by repressing inflammation and apoptosis.
Sujet(s)
Arachidonate 12-lipoxygenase/génétique , Régulation de l'expression des gènes , Traumatismes de la moelle épinière/génétique , Traumatismes de la moelle épinière/prévention et contrôle , Moelle spinale/enzymologie , Animaux , Apoptose/effets des médicaments et des substances chimiques , Apoptose/génétique , Arachidonate 12-lipoxygenase/déficit , Protéines de liaison au calcium/génétique , Protéines de liaison au calcium/métabolisme , Caspase-3/génétique , Caspase-3/métabolisme , Cytochromes c/génétique , Cytochromes c/métabolisme , Protéine gliofibrillaire acide/génétique , Protéine gliofibrillaire acide/métabolisme , Lipopolysaccharides/pharmacologie , Mâle , Souris , Souris de lignée C57BL , Souris knockout , Protéines des microfilaments/génétique , Protéines des microfilaments/métabolisme , Inhibiteur alpha de NF-KappaB/génétique , Inhibiteur alpha de NF-KappaB/métabolisme , Facteur de transcription NF-kappa B/génétique , Facteur de transcription NF-kappa B/métabolisme , Névroglie/effets des médicaments et des substances chimiques , Névroglie/métabolisme , Névroglie/anatomopathologie , Poly(ADP-ribose) polymerases/génétique , Poly(ADP-ribose) polymerases/métabolisme , Transduction du signal , Moelle spinale/anatomopathologie , Traumatismes de la moelle épinière/enzymologie , Traumatismes de la moelle épinière/anatomopathologieRÉSUMÉ
AIMS: Usually, spinal cord injury (SCI) develops into a glial scar containing extracellular matrix molecules including chondroitin sulfate proteoglycans (CSPGs). Chondroitinase ABC (ChABC), from Proteus vulgaris degrading the glycosaminoglycan (GAG) side chains of CSPGs, offers the opportunity to improve the final outcome of SCI. However, ChABC usage is limited by its thermal instability, requiring protein structure modifications, consecutive injections at the lesion site, or implantation of infusion pumps. METHODS: Aiming at more feasible strategy to preserve ChABC catalytic activity, we assessed various stabilizing agents in different solutions and demonstrated, via a spectrophotometric protocol, that the 2.5 mol/L Sucrose solution best stabilized ChABC as far as 14 days in vitro. RESULTS: ChABC activity was improved in both stabilizing and diluted solutions at +37°C, that is, mimicking their usage in vivo. We also verified the safety of the proposed aqueous sucrose solution in terms of viability/cytotoxicity of mouse neural stem cells (NSCs) in both proliferating and differentiating conditions in vitro. Furthermore, we showed that a single intraspinal treatment with ChABC and sucrose reduced reactive gliosis at the injury site in chronic contusive SCI in rats and slightly enhanced their locomotor recovery. CONCLUSION: Usage of aqueous sucrose solutions may be a feasible strategy, in combination with rehabilitation, to ameliorate ChABC-based treatments to promote the regeneration of central nervous system injuries.
Sujet(s)
Astrocytes/effets des médicaments et des substances chimiques , Chondroitine ABC lyase/pharmacologie , Gliose/traitement médicamenteux , Neuroprotecteurs/pharmacologie , Traumatismes de la moelle épinière/traitement médicamenteux , Animaux , Astrocytes/enzymologie , Astrocytes/anatomopathologie , Cellules cultivées , Chondroitine ABC lyase/isolement et purification , Chondroitine ABC lyase/métabolisme , Maladie chronique , Modèles animaux de maladie humaine , Stabilité enzymatique/effets des médicaments et des substances chimiques , Gliose/enzymologie , Gliose/anatomopathologie , Souris , Cellules souches neurales/effets des médicaments et des substances chimiques , Cellules souches neurales/enzymologie , Cellules souches neurales/anatomopathologie , Neuroprotecteurs/isolement et purification , Neuroprotecteurs/métabolisme , Proteus vulgaris/enzymologie , Répartition aléatoire , Rat Sprague-Dawley , Traumatismes de la moelle épinière/enzymologie , Traumatismes de la moelle épinière/anatomopathologie , Régénération de la moelle épinière/effets des médicaments et des substances chimiquesRÉSUMÉ
OBJECTIVE: To investigate the use of surrogate plasma fatty acid analysis to provide further insights into the underlying adiposity and the development of metabolic syndrome in men with spinal cord injury (SCI). DESIGN: Case-control, cross-sectional study. SETTING: Community-based individuals with spinal cord injury and healthy controls. PARTICIPANTS: Twenty men with SCI age, height and weight matched with 20 able-bodied controls. OUTCOME MEASURES: Lean tissue (LTM) and fat mass (FM) were determined using dual energy X-ray absorptiometry. Fasting blood samples were taken for analysis of fatty acids, adiponectin, insulin, glucose and leptin. Enzymatic indices were calculated using relevant fatty acids. RESULTS: Total FM, leptin, stearoyl coenzyme A desaturase (SCD) Δ9 (SCD-16, 16:1/16:0, and SCD-18, 18:1/18:0) indices and Δ6 desaturase index were significantly higher (P < 0.05) in the SCI group than the controls. Significant differences between the groups was observed for several individual fatty acids. Correlational analysis revealed a different pattern between blood biomarkers and indices of SCDs, de novo lipogenesis and elongase. Associations between the desaturase and elongase indices and biomarkers in the controls followed those reported elsewhere for able bodied participants; the same associations were not observed in the SCI group. CONCLUSION: We have identified disturbances in fatty acid biosynthesis in SCI individuals likely associated with the development of adipose tissue below the lesion and a decrease in LTM. Loss of LTM may disturb the normal skeletal muscle-fatty acid metabolic processes leading to the disruption of metabolic homeostasis, previously identified in persons with SCI.
Sujet(s)
Adiponectine/sang , Adiposité/physiologie , Glycémie/métabolisme , Fatty acid desaturases/métabolisme , Fatty acid elongases/métabolisme , Acides gras/sang , Insuline/sang , Leptine/sang , Traumatismes de la moelle épinière/métabolisme , Adolescent , Adulte , Études cas-témoins , Études transversales , Humains , Mâle , Adulte d'âge moyen , Traumatismes de la moelle épinière/sang , Traumatismes de la moelle épinière/enzymologie , Jeune adulteRÉSUMÉ
OBJECTIVE: To investigate the effect of microRNA-210 on the spinal cord injury (SCI) and its underlying mechanism. MATERIALS AND METHODS: The mouse SCI model was established. Mice were randomly assigned into 4 groups, namely the sham operation group (sham group), surgery group (SCI group), surgery+NC group (SCI+NC group) and surgery+microRNA-210 overexpression group (SCI+microRNA-210 mimics group). The mRNA levels of microRNA-210 and the key genes in the JAK-STAT pathway of the four groups were detected by Real-Time Polymerase Chain Reaction (RT-PCR) at different time points. Protein levels of JAK2 and STAT3 in mice of the four groups were detected by Western blot. To investigate the role of microRNA-210 in SCI recovery, changes in the motor function of mice were detected. RESULTS: Grip strengths of right and left forelimbs in mice from the sham group were temporarily decreased at the early stage after surgery, which were gradually recovered to the preoperative levels on the 3rd postoperative day. However, mice in SCI group were unable to complete the grip strength determination at the early stage after surgery. Mice in SCI group were capable of grasping on the 7th postoperative day. Besides, grip strengths of mice in SCI group were remarkably lower than those of sham group until the end-point (on the 50th day). Furthermore, mRNA levels of microRNA-210 in mice of SCI group were decreased in a time-dependent manner (p<0.05). Higher grip strengths were observed in mice of SCI+microRNA-210 mimics group in comparison with those of SCI group and SCI+NC group (p<0.05). In addition, Western blot showed that protein levels of JAK2 and STAT3 in mice of SCI group were increased in a time-dependent manner (p<0.05). Moreover, protein levels of JAK2, STAT3, and MCP-1 in mice of SCI+NC group were remarkably higher than those in the sham group and SCI+microRNA-210 mimics group (p<0.05). CONCLUSIONS: MicroRNA-210 is down-regulated in SCI mice. Grip strengths of SCI mice can be recovered after microRNA-210 overexpression via inhibiting inflammatory response by the JAK-STAT pathway.
Sujet(s)
Inflammation/enzymologie , Kinase Janus-2/métabolisme , microARN/métabolisme , Facteur de transcription STAT-3/métabolisme , Traumatismes de la moelle épinière/enzymologie , Moelle spinale/enzymologie , Animaux , Comportement animal , Modèles animaux de maladie humaine , Inflammation/génétique , Inflammation/physiopathologie , Souris , microARN/génétique , Force musculaire , Récupération fonctionnelle , Transduction du signal , Moelle spinale/physiopathologie , Traumatismes de la moelle épinière/génétique , Traumatismes de la moelle épinière/physiopathologieRÉSUMÉ
BACKGROUND The correlation between serum concentration of neuron specific enolase (NSE), S100B, and the prognosis of patients with acute spinal cord injury (ASCI) remains controversial. MATERIAL AND METHODS Sixty patients with confirmed diagnosis of ASCI were recruited for this study from February 2015 to January 2017. The serum level of NSE and S100B were dynamically measured: on the day of injury and for 2 weeks. The 60 cases were divided into Group A (1 or more than 1 ASIA grade improved at 6 months after the injury) and Group B (ASIA grades changed <1 at 6 months after the injury). The serum level of the 2 groups were compared at different time points. And the prognostic value of serum NSE and S100B as biomarkers in patients with ASCI were calculated by Bayes theorem. RESULTS The serum levels of NSE in Groups A and B on the 2nd day of injury reached a peak at 66.80±13.76 g/L and 98.87±20.12 µg/L, respectively, and then declined gradually. On the 14th day of injury, the serum levels of NSE in both groups were 21.23±8.45 and 39.32±16.31 µg/L, respectively, which were much lower than those on the 2nd day (P<0.05). The serum levels of S100B in Groups A and B rose after the injury and reached a peak on the 4th day of injury. Then, the levels declined gradually to 1.14±0.64 and 1.97±0.98 µg/L, respectively, 2 weeks after the injury. Serum levels of NSE and S100B were good biomarkers for predicting the prognosis of ASCI patients with the sensitivity of 74.35% and 71.79%, the specificity of 71.43% and 66.67%. The cutoff value for serum NSE and S100B were 29.07 µg/L and 1.67 µg/L respectively. The AUCs were 0.78 (95% CI: 0.66-0.89) and 0.76 (95% CI: 0.63-0.89) respectively for serum NSE and S100B. CONCLUSIONS Serum levels of NSE and S100B protein can reflect the degree of spinal cord injury and could be potential biomarkers for the prognosis of acute spinal cord injury.