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1.
Natl Med J India ; 37(1): 9-12, 2024.
Article de Anglais | MEDLINE | ID: mdl-39096226

RÉSUMÉ

Background We assessed the efficacy and safety of management of morbidly adherent placenta by the transfundal uterine incision approach. As a secondary outcome measure, we compared ultrasound and magnetic resonance imaging (MRI) for the diagnosis of adherent placenta. Methods We retrospectively analysed the records of 5 years of women with adherent placenta. Twenty-five women with an antenatal diagnosis of placenta increta and percreta operated by transfundal uterine incision were included. Blood loss, transfusion requirements, operative injuries, and maternal and neonatal intensive care unit (ICU) stay were compared among three different types of adherent placenta. Surgical and other outcome measures were also analysed. Results On antenatal screening with ultrasound, an accurate diagnosis could be achieved in all cases of increta and two-thirds of percreta. Antenatal diagnosis by MRI detected 93.3% of increta and all percreta cases. The mean (SD) gestation at delivery was 34 (4.9) weeks in accreta, 34.9 (2.7) weeks in increta and 31 (4.8) weeks in percreta patients. The mean blood loss encountered intraoperatively was 1012.5 (193.1) ml, 1566.67 (566.52) ml and 1591.67 (629.61) ml in accreta, increta and percreta patients, respectively. Inadvertent bladder injury occurred in 3 women who had placenta percreta invading the bladder. There was no long-term morbidity and no mortality. Conclusion Transfundal incision for delivery of baby is associated with the advantage of avoiding the placenta thereby minimizing blood loss.


Sujet(s)
Placenta accreta , Centres de soins tertiaires , Humains , Femelle , Placenta accreta/chirurgie , Placenta accreta/imagerie diagnostique , Études rétrospectives , Grossesse , Centres de soins tertiaires/statistiques et données numériques , Adulte , Imagerie par résonance magnétique , Perte sanguine peropératoire/statistiques et données numériques , Perte sanguine peropératoire/prévention et contrôle , Résultat thérapeutique , Échographie prénatale , Utérus/chirurgie , Utérus/imagerie diagnostique
2.
J Matern Fetal Neonatal Med ; 37(1): 2382309, 2024 Dec.
Article de Anglais | MEDLINE | ID: mdl-39098848

RÉSUMÉ

OBJECTIVE: Congenital uterine anomalies during pregnancy increase the risk of pregnancy complications such as miscarriage, preterm delivery, fetal malpresentation, cesarean delivery, and fetal growth restriction. However, few studies have examined uterine anomalies in relation to perinatal complications other than those mentioned above. We investigated the association between pregnancies complicated by congenital uterine anomalies and various perinatal outcomes at our institution. METHODS: This retrospective cohort study was conducted from January 2009 to May 2021. We included cases of uterine anomalies, such as septate, bicornuate, unicornuate, and didelphic uterus. First, the perinatal complications and neonatal outcomes were compared between pregnancies complicated by uterine anomalies and those with normal uteri. Second, we conducted an analysis based on the type of uterine anomalies classified into two groups: the minor anomaly group consisted of anomalies limited to the uterine cavity, such as the septate uterus, whereas the major anomaly group included anomalies affecting the uterine shape, such as bicornuate, unicornuate, and didelphic uterus. We compared the incidence of perinatal complications among the major anomaly, minor anomaly, and normal uterus groups. RESULTS: During the study period, 45 pregnancies were complicated with uterine anomalies. The minor anomaly group included 11 patients and the major anomaly group included 34 patients. The incidence of fetal malpresentation was significantly higher in the uterine anomaly group than in the normal uterus group (18% vs. 3.7%, p = .04). Furthermore, the frequency of abnormal placental cord insertion was significantly higher in the uterine anomaly group (16% vs. 3.7%, p = .01). Examination based on the type of uterine anomaly revealed significant differences in cervical incompetence, malpresentation, cesarean section, and abnormal placental cord insertion. Cervical incompetence was more likely in patients with minor anomalies. In contrast, fetal malpresentation, cesarean section, and abnormal placental cord insertion were more likely in the major anomaly group. CONCLUSIONS: In addition to the findings reported in previous studies, abnormal placental cord insertion was more frequent in pregnancies complicated by uterine anomalies.


Sujet(s)
Complications de la grossesse , Malformations urogénitales , Utérus , Humains , Femelle , Grossesse , Études rétrospectives , Utérus/malformations , Adulte , Malformations urogénitales/épidémiologie , Malformations urogénitales/complications , Complications de la grossesse/épidémiologie , Issue de la grossesse/épidémiologie , Nouveau-né , Cordon ombilical/malformations
3.
J Med Case Rep ; 18(1): 361, 2024 Aug 03.
Article de Anglais | MEDLINE | ID: mdl-39095912

RÉSUMÉ

INTRODUCTION: Herlyn-Werner-Wunderlich syndrome , a rare Müllerian ducts congenital disease, is characterized by a diphtheritic uterus, blind hemivagina, and ipsilateral renal agenesis. Diagnosis is at young age by ultrasound and magnetic resonance imaging, and the prognosis is good. Usually, complications evolve endometriosis and secondary pelvic inflammation. CASE REPORT: A 40-year-old female patient, Brazilian, white, primigravida, diagnosed at 30 years with a didelphic uterus on ultrasound, and 4 years later, with a left ovarian endometrioma, multiple ovarian cysts, and left renal agenesis on magnetic resonance imaging. Subsequently, due to dyspareunia and a feeling of swelling, the patient underwent transvaginal ultrasound with bowel preparation, and a hematocolpos was found and Herlyn-Werner-Wunderlich syndrome was suspected; 10 years after the diagnosis she had a planned pregnancy. She presented frequent contractions following the 15th week of pregnancy and fortunately there were no complications or premature labor. Labor was inducted at 40 weeks and 6 days without progress and a cesarean section was indicated and performed without complications. Herlyn-Werner-Wunderlich syndrome often goes unnoticed, leading to inadequate treatment. Individuals with Herlyn-Werner-Wunderlich syndrome commonly face fertility issues, such as high miscarriage rate (21-33%), and obstetric complications, such as spontaneous abortions (40% risk), intrauterine growth restriction, postpartum hemorrhage, increased fetal mortality, preterm delivery (21-29%), and elevated rates of cesarean sections. In addition, there is higher susceptibility of developing endometriosis, especially with hemivaginal obstruction, and pelvic adhesions. CONCLUSION: Early diagnosis enables timely treatment and, consequently, fewer complications. Still, when these factors are absent, vaginal birth may still be possible. The true prevalence and incidence of complications related to Herlyn-Werner-Wunderlich syndrome are still unknown.


Sujet(s)
Endométriose , Humains , Femelle , Adulte , Endométriose/complications , Grossesse , Utérus/malformations , Utérus/imagerie diagnostique , Césarienne , Rein/malformations , Canaux de Müller/malformations , Malformations multiples , Malformations/diagnostic , Malformations/imagerie diagnostique , Vagin/malformations , Complications de la grossesse , Maladies du rein/congénital , Maladies du rein/diagnostic
4.
Sci Rep ; 14(1): 17784, 2024 Aug 01.
Article de Anglais | MEDLINE | ID: mdl-39090384

RÉSUMÉ

The position of the internal os of the cervix reported in the literature was inconsistent on MRI images. Additionally, the practical impactful data influencing the internal os located by MRI is limited. We aimed to confirm the position of the internal os of the cervix on MRI images, and the influencing factors locating the the internal os by MRI. A single-center retrospective study was conducted. Data from 175 patients who underwent total hysterectomy for stage I endometrial cancer were collected. The internal os of the cervix is positioned as the starting point for measuring the length of the cervix on MRI images. On dynamic contrast-enhanced MRI (DCE-MRI), the section formed by the enhancement difference between the uterus and cervix, and on T2-weighted imaging(T2WI), the section formed by the physiological curvature of the uterus and the low signal intensity of the cervical stroma were used as starting points. The results showed no statistically significant difference compared with the removed uterus specimens (p = 0.208, p = 0.571, p = 0.804). A history of cesarean section(p < 0.001), irregular vaginal bleeding for more than three months(p < 0.001), cervical adenomyosis(p = 0.043), and premenopause(p = 0.001) were not conducive to locating the internal os of the cervix by MRI. Our findings provide valuable information and confirm the position of the internal os of the cervix on MRI images, and the several important infuencing factors. We hope that some patients will benefit from our study.


Sujet(s)
Col de l'utérus , Imagerie par résonance magnétique , Humains , Femelle , Imagerie par résonance magnétique/méthodes , Col de l'utérus/imagerie diagnostique , Col de l'utérus/anatomopathologie , Adulte d'âge moyen , Études rétrospectives , Sujet âgé , Adulte , Hystérectomie , Tumeurs de l'endomètre/imagerie diagnostique , Tumeurs de l'endomètre/anatomopathologie , Tumeurs de l'endomètre/chirurgie , Utérus/imagerie diagnostique , Utérus/anatomopathologie , Utérus/chirurgie
5.
BMJ Case Rep ; 17(7)2024 Jul 01.
Article de Anglais | MEDLINE | ID: mdl-38955380

RÉSUMÉ

We describe the case of a woman with mild endometriosis and Allen-Masters syndrome after in vitro fertilisation (IVF), presenting at 7 weeks 2 days gestation with abdominal pain. A transvaginal ultrasound revealed a gestational sac with a non-viable fetus near the right ovary. Laparoscopy was performed due to escalating abdominal pain which revealed a ruptured ectopic pregnancy at the right uterosacral ligament (USL) and blood in the pouch of Douglas. A peritoneal incision along the USL facilitated drainage and removal of the ectopic pregnancy. A pathological investigation described the presence of endometrial tissue directly adjacent to products of conception, which suggested a retroperitoneal implantation that may have been facilitated by the presence of an endometriotic lesion. This case underscores the distinctive clinical trajectory of unconventional ectopic pregnancies, provides novel insights into the pathophysiological mechanism of ectopic implantation and underscores the crucial role of comprehensive patient assessment during IVF and subsequent pregnancy in ensuring effective management.


Sujet(s)
Fécondation in vitro , Ligaments , Grossesse extra-utérine , Humains , Femelle , Grossesse , Fécondation in vitro/effets indésirables , Grossesse extra-utérine/chirurgie , Grossesse extra-utérine/diagnostic , Adulte , Endométriose/complications , Endométriose/chirurgie , Douleur abdominale/étiologie , Laparoscopie , Syndrome , Utérus/chirurgie
6.
Zhonghua Fu Chan Ke Za Zhi ; 59(6): 440-446, 2024 Jun 25.
Article de Chinois | MEDLINE | ID: mdl-38951079

RÉSUMÉ

Objective: To investigate the effect of autologous platelet-rich plasma (PRP) perfusion on the levels of cytokines in uterine drainage fluid in patients with moderate to severe intrauterine adhesions (IUA) following hysteroscopic adhesiolysis. Methods: Thirty patients with moderate to severe IUA who underwent hysteroscopic adhesiolysis at Beijing Obstetrics and Gynecology Hospital, Capital Medical University from November 2020 to March 2021 were randomly divided into two groups: the PRP group (15 patients with placement of intrauterine-suitable balloons and PRP infusion) and the control group (15 patients with placement of intrauterine-suitable balloons only). For all patients, the channel switch was opened 48 hours after the surgery. The drainage fluid of the uterine cavity was collected using syringes through the proximal end of the drainage channel switch at 24 hours after the surgery and through the drainage channel directly at 48, 72, 96, and 120 hours after the surgery, and the levels of related cytokines including platelet-derived growth factor BB (PDGF-BB), vascular endothelial growth factor A (VEGF-A), insulin-like growth factor 1 (IGF-1) and transforming growth factor-ß1 (TGF-ß1) in the drainage fluid of the uterine cavity were evaluated, respectively. Results: (1) The changes in volumes of uterine cavity drainage fluid: the total drainage fluid volumes of the PRP group and the control group in 120 hours after the surgery were (21.8±2.9) and (22.7±2.7) ml, respectively, and there was no statistically significant difference between the two groups (t=-0.847, P>0.05). No significant differences were found in the volumes of drainage fluid between the two groups at 72, 96, and 120 hours after the surgery (all P>0.05). (2) Variation in cytokine levels in the uterine cavity drainage fluid: ① PDGF-BB: median PDGF-BB levels at 24 and 48 hours after the surgery in the PRP group (6.6 and 9.6 µg/L, respectively) were significantly higher than those in the control group (4.7 and 2.7 µg/L, respectively; all P<0.05). There were no significant differences in PDGF-BB levels between the two groups at 72, 96, and 120 hours after the surgery (all P>0.05). ② VEGF-A: median VEGF-A levels at 24 and 48 hours after the surgery in the PRP group (3.5 and 2.8 µg/L, respectively) were significantly higher than those in the control group (1.6 and 1.2 µg/L, respectively; all P<0.05). There were no significant differences in VEGF-A levels between the two groups at 72, 96, and 120 hours after the surgery (all P>0.05). ③ IGF-1: median IGF-1 level at 48 hours after the surgery in the PRP group was significantly higher than that in the control group (39.5 vs 8.6 µg/L, P<0.05). No significant differences were found in IGF-1 levels at 24, 72, 96, and 120 hours after the surgery between the two groups (all P>0.05). ④ TGF-ß1: There were no significant differences in TGF-ß1 levles between the two groups at 24, 48, 72, 96, and 120 hours after the surgery (all P>0.05). Conclusions: PRP perfusion following hysteroscopic adhesiolysis may increase the levels of PDGF-BB, VEGF-A, and IGF-1 in the uterine cavity drainage fluid, which plays a beneficial role in improving wound microvascular formation, reducing adhesion reformation, and promoting endometrial regeneration and repair.


Sujet(s)
Cytokines , Drainage , Hystéroscopie , Plasma riche en plaquettes , Humains , Femelle , Adhérences tissulaires , Hystéroscopie/méthodes , Adulte , Cytokines/métabolisme , Drainage/méthodes , Maladies de l'utérus/chirurgie , Maladies de l'utérus/étiologie , Utérus , Facteur de croissance endothéliale vasculaire de type A/métabolisme , Facteur de croissance IGF-I/métabolisme , Bécaplermine
7.
Reprod Domest Anim ; 59(7): e14665, 2024 Jul.
Article de Anglais | MEDLINE | ID: mdl-38973694

RÉSUMÉ

This study aims to conduct a comparative analysis of the uterotubal junction in two distinct mammalian species, the bovine (Bos taurus) and the dromedary camel (Camelus dromedarius), focusing on histological and histomorphometric parameters. Uterotubal junction (UTJ) was dissected from 8 cows and 12 camels with dominant follicles, and processed for H&E staining for histology, and histomorphometry examination. The results showed that the camel uterotubal junction papillae (UTJP) existed only in camels and was completely absent in cattle. Histologically, the cow UTJ appears with a star-shaped lumen, and the mucosa is lined by a simple columnar epithelium with ciliated and non-ciliated cells. Superficial (SG) and deep glands (DG) were abundant in the submucosa. Camel UTJP is a conical structure, it has a pale yellowish colour, 0.5 ± 0.2 cm height, and 0.3 ± 0.1 cm width, and it has a distinct sphincter at the tip towards the uterine lumen. In the camel UTJP, the lumen is wider, and the mucosa showed large multiple folds lined with ciliated and non-ciliated cells in a pseudostratified columnar epithelium. The submucosa showed no mucosal glands. Lumen area, lumen epithelial height, luminal epithelial density, the thickness of the muscular layer, number of folds, fold height, epithelial height, fold area and epithelial perimeter were higher in camel UTJP (p < .001) than cow UTJ, while the total endosalpinx area and glandular epithelial density were lower in camel UTJP (p < .001) than in cow UTJ. In conclusion, this study elucidates potential variations in the uterotubal junction between bovines and dromedary camels, providing valuable insights into their reproductive adaptations. The epithelial lining, absence of glands and the thick layer of tunica muscularis might indicate that camel UTJP could play a mechanical role in selecting spermatozoa and assisting the hatching of blastocysts during their passage into the uterus.


Sujet(s)
Chameaux , Utérus , Animaux , Chameaux/anatomie et histologie , Femelle , Bovins/anatomie et histologie , Utérus/anatomie et histologie , Trompes utérines/anatomie et histologie
8.
Tissue Cell ; 89: 102456, 2024 Aug.
Article de Anglais | MEDLINE | ID: mdl-38991273

RÉSUMÉ

Polycystic ovary syndrome (PCOS) is the most common gynaecological, endocrine disorder that occurs during reproductive age and is a significant cause of anovulatory infertility. Letrozole is an aromatase inhibitor which negates the action of the aromatase enzyme, which results in the buildup of male hormones (testosterone) in the females, causing hyperandrogenism, which is a hallmark of Polycystic Ovarian Syndrome. Mifepristone (RU486) is a progestin antagonist that acts to arrest the actions of the progesterone hormone, resulting in follicular atresia and anovulation. DHEA is an androgen which was also administered in a bid to cause hyperandrogenism in the rats.This study aimed to evaluate the effects of these hormones on the cytoarchitecture of the ovaries and uterus to assess their various PCOS-like histological features.Animals were grouped mainly into three: Letrozole, Mifepristone and DHEA groups, which were further divided into two subgroups each, administered low and high doses of letrozole orally, Mifepristone and Dehydroepiandosterone (DHEA) subcutaneously. Each of the subgroups also had a comparison control group. Following the completion of administration, the Wistar rats were euthanized, and their ovaries and uterus were collected for histological analysis.Increased proliferation of ovarian follicles was noted in the treated groups compared to control, as well as thickening of the endometrial layer.


Sujet(s)
Modèles animaux de maladie humaine , Létrozole , Mifépristone , Ovaire , Syndrome des ovaires polykystiques , Rat Wistar , Utérus , Animaux , Femelle , Syndrome des ovaires polykystiques/anatomopathologie , Syndrome des ovaires polykystiques/induit chimiquement , Rats , Létrozole/pharmacologie , Ovaire/anatomopathologie , Ovaire/effets des médicaments et des substances chimiques , Mifépristone/pharmacologie , Utérus/anatomopathologie , Utérus/effets des médicaments et des substances chimiques , Follicule ovarique/effets des médicaments et des substances chimiques , Follicule ovarique/anatomopathologie , Follicule ovarique/métabolisme , Déhydroépiandrostérone/pharmacologie
9.
Front Immunol ; 15: 1411315, 2024.
Article de Anglais | MEDLINE | ID: mdl-38979410

RÉSUMÉ

Pregnancy is a fascinating immunological phenomenon because it allows allogeneic fetal and placental tissues to survive inside the mother. As a component of innate immunity with high inflammatory potential, the complement system must be tightly regulated during pregnancy. Dysregulation of the complement system plays a role in pregnancy complications including pre-eclampsia and intrauterine growth restriction. Complement components are also used as biomarkers for pregnancy complications. However, the mechanisms of detrimental role of complement in pregnancy is poorly understood. C5a is the most potent anaphylatoxin and generates multiple immune reactions via two transmembrane receptors, C5aR1 and C5aR2. C5aR1 is pro-inflammatory, but the role of C5aR2 remains largely elusive. Interestingly, murine NK cells have been shown to express C5aR2 without the usual co-expression of C5aR1. Furthermore, C5aR2 appears to regulate IFN-γ production by NK cells in vitro. As IFN-γ produced by uterine NK cells is one of the major factors for the successful development of a vital pregnancy, we investigated the role anaphylatoxin C5a and its receptors in the establishment of pregnancy and the regulation of uterine NK cells by examinations of murine C5ar2-/- pregnancies and human placental samples. C5ar2-/- mice have significantly reduced numbers of implantation sites and a maternal C5aR2 deficiency results in increased IL-12, IL-18 and IFN-γ mRNA expression as well as reduced uNK cell infiltration at the maternal-fetal interface. Human decidual leukocytes have similar C5a receptor expression patterns showing clinical relevance. In conclusion, this study identifies C5aR2 as a key contributor to dNK infiltration and pregnancy success.


Sujet(s)
Cellules tueuses naturelles , Souris knockout , Récepteur à l'anaphylatoxine C5a , Utérus , Récepteur à l'anaphylatoxine C5a/génétique , Récepteur à l'anaphylatoxine C5a/métabolisme , Femelle , Animaux , Grossesse , Souris , Utérus/immunologie , Humains , Cellules tueuses naturelles/immunologie , Cellules tueuses naturelles/métabolisme , Placenta/immunologie , Placenta/métabolisme , Complément C5a/immunologie , Complément C5a/métabolisme , Souris de lignée C57BL , Interféron gamma/métabolisme , Interféron gamma/immunologie
10.
Anim Reprod Sci ; 267: 107548, 2024 Aug.
Article de Anglais | MEDLINE | ID: mdl-38959672

RÉSUMÉ

We aimed to determine associations between experimentally impaired uterine clearance or treatment with ecbolic drugs on luteal development in estrous mares after insemination. In a crossover design, eight mares were treated with saline (CON), clenbuterol (CLEN), oxytocin (OXY) and carbetocin (CARB) from the day of first insemination until 2 days after ovulation. Between treatments, the mares rested for one cycle. Estrous mares were examined for the presence of free intrauterine fluid by transrectal ultrasound. Endometrial swabs for cytology and bacteriology were collected on days 1 and 14. Blood samples were collected daily before AI until day 14 after ovulation for determination of progesterone and PGF2α metabolites (PGFM). Differences between treatments were compared by a general linear model for repeated measures (SPSS 29). One mare was excluded because of a uterine infection in the control cycle. In all other mares, only minor amounts of free intrauterine fluid were present after insemination and decreased over time (P<0.05) with no treatment x time interaction. There was no effect of treatment on polymorphonucleated cells (PMN) in endometrial cytology after ovulation or PGFM secretion. Progesterone release from day 1-14 as well as pregnancy rate and conceptus size on day 14 was not influenced by treatment. In conclusion, treatment with clenbuterol does not impair uterine clearance in estrous mares resistant to endometritis. Repeated injection of the oxytocin analogue carbetocin during the early postovulatory period is not detrimental to corpus luteum function and can be recommended to enhance uterine clearance.


Sujet(s)
Ovulation , Ocytocine , Animaux , Femelle , Equus caballus , Ocytocine/pharmacologie , Ocytocine/analogues et dérivés , Ovulation/effets des médicaments et des substances chimiques , Grossesse , Corps jaune/effets des médicaments et des substances chimiques , Utérus/effets des médicaments et des substances chimiques , Études croisées , Maladies des chevaux/traitement médicamenteux , Insémination artificielle/médecine vétérinaire , Progestérone/pharmacologie , Progestérone/sang , Endomètre/effets des médicaments et des substances chimiques , Endomètre/métabolisme , Endométrite/médecine vétérinaire , Endométrite/traitement médicamenteux
11.
Asian J Endosc Surg ; 17(4): e13361, 2024 Oct.
Article de Anglais | MEDLINE | ID: mdl-39039742

RÉSUMÉ

A 27-year-old nulliparous woman presented with a feeling of fullness in the lower abdomen and abdominal pain. A left ovarian tumor, uterus didelphys, left renal agenesis, and left vaginal atresia were observed on imaging. The ovarian tumor was presumed to have caused the abdominal pain, and an abdominal left adnexectomy was performed. After 3 months, she reported severe lower abdominal pain during menstruation. Transvaginal ultrasonography revealed uterine enlargement. After 17 days, the patient presented with abdominal pain and fever. She was diagnosed with peritonitis due to infection and left uterine hematometra. Because she did not improve with antibiotic treatment, left laparoscopic hysterectomy was performed. Subsequently, she did not experience the lower abdominal pain. Appropriate diagnosis and treatment based on the morphology of the reproductive tract and symptoms must be considered in patients with Herlyn-Werner-Wunderlich syndrome. Treatment must permit the outflow of menstrual blood.


Sujet(s)
Hématométrie , Utérus , Vagin , Humains , Femelle , Adulte , Syndrome , Vagin/malformations , Vagin/chirurgie , Utérus/malformations , Utérus/chirurgie , Hématométrie/étiologie , Hématométrie/diagnostic , Rein/malformations , Rein/imagerie diagnostique , Tumeurs de l'ovaire/chirurgie , Tumeurs de l'ovaire/complications , Tumeurs de l'ovaire/diagnostic , Malformations multiples/chirurgie , Hystérectomie , Malformations/chirurgie , Malformations/diagnostic
12.
Zhongguo Zhong Yao Za Zhi ; 49(11): 3061-3069, 2024 Jun.
Article de Chinois | MEDLINE | ID: mdl-39041166

RÉSUMÉ

In order to study the toxic effect and mechanism of triptolide(TP) on the reproductive system of female rats with Ⅱ type collagen induced arthritis(CIA), 50 SD rats were randomly divided into normal control group, CIA model group, and three groups receiving TP tablets at clinically equivalent doses of 0. 5, 1, and 2 times, respectively(with TP dosages of 3. 75, 7. 5, and 15 µg·kg~(-1)·d~(-1)), each comprising 10 rats. Intragastric administration was started on the day after the first immunization, once a day, for 42 days.The results were taken on the 21st and 42nd days to calculate the uterine and ovarian organ indexes; pathological and morphological changes in uterus and ovaries were observed under a light microscope; and the levels of estradiol(E_2) and cytochrome P450A1(aromatase,CYP19A1) in ovarian homogenate were detected by ELISA. Furthermore, immunohistochemistry was employed to detect the expression levels of transforming growth factor ß3( TGFß3) pathway-related proteins, mothers against decapentaplegic homolog 3(Smad3) and steroidogenic factor-1(SF-1) in ovarian tissues. In vitro, the mouse Chinese hamster ovary(CHO) cell line was established, and after 24 hours of TP administration(30, 60, 120 nmol·L~(-1)), cell proliferation was detected by the thiazolyl blue tetrazolium bromide(MTT) method, apoptosis by the flow cytometry, and TGFß3, Smad3 and SF-1 protein expression in cells by the Western blot method, and the nuclear entry of SF-1 was detected by immunofluorescence. The results showed that compared with the CIA model group, all TP administration groups showed decreased number of uterine glands, total follicles, mature follicles, and corpus luteum on days 21 and 42 of administration, but there was no statistical difference, and only the administration of 2 times the clinically equivalent dose of TP could significantly increase the number of atretic follicles at 42 days of administration. TP at 3. 75 µg·kg-1·d-1significantly reduced the level of E_2 at 21 days of administration and the expression of TGFß3 and Smad3 factors in ovarian tissues,but had no significant effect on the rate-limiting enzyme in estrogen synthesis CYP19A1. TP at 7. 5 and 15 µg·kg~(-1)·d~(-1) significantly reduced the expression of SF-1 regardless of administration for 21 days or 42 days. TP can significantly promote ovarian cell apoptosis in vitro, with apoptosis mainly concentrated in the late stage of apoptosis after 24 hours of administration. In addition, 60 nmol·L~(-1) TP significantly reduced the protein expression of TGFß3, Smad3 and SF-1 in a dose-dependent manner. In summary, intragastric administration of TP at less than 2 times the clinically equivalent dose for 21 days and 42 days did not cause obvious reproductive damage to the uterus and ovarian tissues of CIA rats, and the number of atretic follicles changed significantly only when the 2 times the clinically equivalent dose was administered for 42 days. TP exerted reproductive toxicity in vivo on reproductive target organs and in vitro on ovarian cells by inhibiting the expression of TGFß3/Smad3/SF-1 pathway.


Sujet(s)
Diterpènes , Composés époxy , Ovaire , Phénanthrènes , Rat Sprague-Dawley , Utérus , Animaux , Femelle , Diterpènes/pharmacologie , Phénanthrènes/toxicité , Rats , Composés époxy/toxicité , Composés époxy/administration et posologie , Ovaire/effets des médicaments et des substances chimiques , Ovaire/métabolisme , Utérus/effets des médicaments et des substances chimiques , Utérus/métabolisme , Collagène de type II/métabolisme , Protéine Smad-3/métabolisme , Protéine Smad-3/génétique , Humains , Reproduction/effets des médicaments et des substances chimiques , Arthrite expérimentale/traitement médicamenteux , Arthrite expérimentale/métabolisme , Oestradiol
13.
J Cell Mol Med ; 28(14): e18550, 2024 Jul.
Article de Anglais | MEDLINE | ID: mdl-39042561

RÉSUMÉ

Endometritis is one of the important causes of infertility. Puerarin (PU) can inhibit oxidative stress and reduce inflammation; however, it is unclear whether PU has a protective effect on the endometritis. In our study, we used Staphylococcus aureus to induce mouse endometritis. The PU group (100 mg/kg PU) and the S. aureus + PU group received daily intraperitoneal injection of PU (25, 50 or 100 mg/kg PU). The results showed that S. aureus significantly increased the levels of MPO, TNF-α, IL-1ß and IL-6 in uterine tissue, and increased the expression of p-p65 and p-IκBα proteins in uterine tissue to induce endometritis in mice (p < 0.05). Furthermore, it has been found that S. aureus promotes the occurrence of ferroptosis by reducing GSH and ATP content, increasing MDA and iron content and reducing GPX4 and SLC7A11 protein expression levels (p < 0.05). S. aureus significantly increase the expression of NLRP3, ASC, caspase-1 and P2X7 proteins in uterine tissue (p < 0.05). However, PU obviously reduced the inflammatory response and reversed the changes of ferroptosis and the expression of P2X7 receptor/NLRP3 pathway associated proteins of the uterus induced by S. aureus (p < 0.05). Taken together, these findings emphasize the protective effect of PU on endometritis by regulating the P2X7 receptor/NLRP3 signalling pathway and inhibiting ferroptosis.


Sujet(s)
Endométrite , Ferroptose , Isoflavones , Protéine-3 de la famille des NLR contenant un domaine pyrine , Récepteurs purinergiques P2X7 , Transduction du signal , Infections à staphylocoques , Staphylococcus aureus , Animaux , Femelle , Protéine-3 de la famille des NLR contenant un domaine pyrine/métabolisme , Isoflavones/pharmacologie , Isoflavones/usage thérapeutique , Ferroptose/effets des médicaments et des substances chimiques , Staphylococcus aureus/pathogénicité , Endométrite/métabolisme , Endométrite/microbiologie , Endométrite/traitement médicamenteux , Endométrite/anatomopathologie , Transduction du signal/effets des médicaments et des substances chimiques , Souris , Récepteurs purinergiques P2X7/métabolisme , Infections à staphylocoques/métabolisme , Infections à staphylocoques/microbiologie , Infections à staphylocoques/traitement médicamenteux , Inflammation/métabolisme , Inflammation/anatomopathologie , Utérus/métabolisme , Utérus/anatomopathologie , Utérus/effets des médicaments et des substances chimiques , Utérus/microbiologie , Stress oxydatif/effets des médicaments et des substances chimiques
14.
Int J Mol Sci ; 25(13)2024 Jul 06.
Article de Anglais | MEDLINE | ID: mdl-39000535

RÉSUMÉ

The receptive phase of the uterus is marked by structural and functional maturation of the endometrium. During this limited time span, the blastocyst competency is superimposed on the receptive endometrium. It is a well-known fact that lipid signalling in early-stage pregnancy has a crucial role in successful embryogenesis. In our study, CD-1 mouse uteri after normal and in vitro fertilization (IVF) were investigated at 6.5, 8.5, and 10.5 days of pregnancy. Matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry and liquid chromatography coupled tandem mass spectrometry were used for identification of phosphatidylcholine (PC) lipid structures. In the embryonal tissues, PC 32:0 and PC 34:0 were increased, while in the antemesometrial (AM) decidua the two 20:4-containing PCs, PC 36:4 and PC 38:4 were increased. In transferred uterus samples, higher expressions of PC 34:0, PC 34:1, PC 34:2, PC 36:1, and PC 36:2 in mesometrial decidua were seen, whereas the two 20:4-containing PCs, PC 36:4 and PC 38:4 showed increased expression in the AM and lateral decidua. This paper shows a significant spatio-temporal change in lipid metabolism during IVF procedures for the first time.


Sujet(s)
Fécondation in vitro , Phosphatidylcholines , Spectrométrie de masse MALDI , Spectrométrie de masse MALDI/méthodes , Femelle , Animaux , Souris , Phosphatidylcholines/métabolisme , Phosphatidylcholines/analyse , Fécondation in vitro/méthodes , Grossesse , Embryon de mammifère/métabolisme , Développement embryonnaire , Utérus/métabolisme , Blastocyste/métabolisme
15.
Reprod Domest Anim ; 59(7): e14671, 2024 Jul.
Article de Anglais | MEDLINE | ID: mdl-39005009

RÉSUMÉ

This study evaluated the relationship between CL features assessed by ultrasound (luteal tissue area and blood flow, BF) or rectal palpation (size), uterine tone (UT), plasma progesterone (P4) concentration on Day 7 (D7) and subsequent pregnancy outcomes in bovine embryo recipients. A total of 163 cows and heifers were included in this study. The expected day of ovulation after the synchronization protocol was designated as D0. On D7, ovaries and uterus were examined by ultrasonography and rectal palpation, and subjective scores (1-3 scale) were assigned for CL size, area and BF, and for UT. Blood samples were collected for further P4 analysis. Each embryo recipient then received a grade I frozen-thawed in vivo-produced blastocyst, which was transferred to the uterine horn ipsilateral to the CL. Pregnancy diagnosis was performed on D35, and the results were retrospectively compared with the assigned scores for CL and UT. We observed a significant (p < .02) interaction between CL size and UT, with a progressive increase in the likelihood of pregnancy for recipients bearing a large CL among those with turgid UT. Ultrasound scoring of the CL using B-mode and Doppler-mode did not significantly predict pregnancy rates on D35 (p < .6 and p < .5, respectively). However, logistic regression analysis revealed a trend towards a quadratic effect (p < .08 and p < .06) indicating that the probability of pregnancy varied according to the area of luteal tissue and P4 concentrations, respectively. No significant (p > .05) association was found between the probability of pregnancy and the BF area of the CL. In summary, UT before embryo transfer may reflect successful recipient synchronization. Elevated P4 levels, assessed by CL size, may offset uterine contractility, mitigating adverse effects. Additionally, the CL area may be more important than its vascularization area when evaluating recipients D7 after ovulation.


Sujet(s)
Transfert d'embryon , Progestérone , Utérus , Femelle , Animaux , Bovins/physiologie , Transfert d'embryon/médecine vétérinaire , Grossesse , Utérus/vascularisation , Utérus/imagerie diagnostique , Progestérone/sang , Échographie/médecine vétérinaire , Corps jaune/physiologie , Taux de grossesse , Synchronisation de l'oestrus , Issue de la grossesse/médecine vétérinaire
16.
Sci Data ; 11(1): 777, 2024 Jul 13.
Article de Anglais | MEDLINE | ID: mdl-39003290

RÉSUMÉ

The ovaries and uterus are crucial reproductive organs in mammals, and their coordinated development ensures the normal development of sexual maturity and reproductive capacity. This study aimed to comprehensively capture the different physiological stages of the goat's sexual maturation by selecting four specific time points. We collected samples of ovarian and uterine tissues from five female Jining Gray goats at each time point: after birth (D1), 2-month-old (M2), 4-month-old (M4), and 6-month-old (M6). By combining transcriptomic sequencing of 40 samples (including rRNA-depleted RNA-seq libraries with 3607.8 million reads and miRNA-seq libraries with 444.0 million reads) and metabolomics analysis, we investigated the transcriptomic mechanisms involved in reproductive regulation in the ovary and uterus during sexual maturation, as well as the changes in metabolites and their functional potential. Additionally, we analyzed blood hormone indices and uterine tissue sections to examine temporal changes. These datasets will provide a valuable reference for the reproductive regulation of the ovary and uterus, as well as the regulation of metabolites during sexual maturation in goats.


Sujet(s)
Capra , Ovaire , Maturation sexuelle , Transcriptome , Utérus , Animaux , Femelle , Capra/génétique , Capra/métabolisme , Utérus/métabolisme , Ovaire/métabolisme , Ovaire/croissance et développement , Métabolome , Métabolomique
17.
Bull Exp Biol Med ; 177(1): 47-50, 2024 May.
Article de Anglais | MEDLINE | ID: mdl-38955852

RÉSUMÉ

Ectonucleotidases play an important role in regulating the level of extracellular nucleotides and nucleosides and are an important part of the regulation of the effects of adenosine and ATP on adenosine and P2 receptors, respectively. We have previously established the ambiguous effect of P2 receptor agonists on the contractile activity of smooth muscle tissue in rats with the valproate model of autism. In this work, HPLC was used to evaluate the activity of ectonucleotidases in the smooth muscle tissues of the internal organs of rats with a valproate model of autism. The activity of ectonucleotidases was significantly higher in the smooth muscle tissues of the duodenum, vas deferens, and bladder, but lower in the ileum and uterus. The results obtained make it possible to compare the activity of ectonucleotidases identified here with changes in P2 receptor-mediated contractility of smooth muscle tissues revealed in our previous experiments.


Sujet(s)
Trouble autistique , Contraction musculaire , Muscles lisses , Vessie urinaire , Acide valproïque , Conduit déférent , Animaux , Rats , Muscles lisses/effets des médicaments et des substances chimiques , Muscles lisses/métabolisme , Acide valproïque/pharmacologie , Trouble autistique/métabolisme , Trouble autistique/induit chimiquement , Trouble autistique/traitement médicamenteux , Mâle , Femelle , Conduit déférent/effets des médicaments et des substances chimiques , Conduit déférent/métabolisme , Vessie urinaire/effets des médicaments et des substances chimiques , Vessie urinaire/métabolisme , Vessie urinaire/enzymologie , Contraction musculaire/effets des médicaments et des substances chimiques , Utérus/effets des médicaments et des substances chimiques , Utérus/métabolisme , Iléum/effets des médicaments et des substances chimiques , Iléum/métabolisme , Iléum/enzymologie , Modèles animaux de maladie humaine , Rat Wistar , Récepteurs purinergiques P2/métabolisme , Adenosine triphosphatases/métabolisme
18.
BMJ Case Rep ; 17(7)2024 Jul 29.
Article de Anglais | MEDLINE | ID: mdl-39074937

RÉSUMÉ

This case report elucidates a scenario involving two sibling sisters born out of consanguineous marriage-one initially presenting with lower respiratory infection, concurrently exhibiting short stature and primary amenorrhoea. Investigation into the primary amenorrhoea unveiled hypergonadotropic hypogonadism, confirmed by the absence of ovaries and a hypoplastic uterus on pelvic MRI. Genetic analysis via whole exome sequencing identified a homozygous variant NM_001282717.2: c.808C>T in the MCM8 gene, located on exon 8 of chromosome 20, inherited in an autosomal recessive manner. The scarcity of primary ovarian insufficiency cases linked to MCM8 highlights the necessity of thoroughly investigating the genetic and clinical consequences of such variants.


Sujet(s)
Protéines de maintenance des minichromosomes , Mutation , Insuffisance ovarienne primitive , Fratrie , Utérus , Humains , Femelle , Insuffisance ovarienne primitive/génétique , Protéines de maintenance des minichromosomes/génétique , Utérus/malformations , Consanguinité , Imagerie par résonance magnétique , , Aménorrhée/génétique , Aménorrhée/étiologie , Malformations urogénitales/génétique , Malformations urogénitales/imagerie diagnostique
19.
Acta Biomater ; 184: 144-155, 2024 Aug.
Article de Anglais | MEDLINE | ID: mdl-38964528

RÉSUMÉ

The integration of barrier materials with pharmacological therapy is a promising strategy to treat intrauterine adhesions (IUAs). However, most of these materials are surgically implanted in a fixed shape and incongruence with the natural mechanical properties of the uterus, causing poor adaptability and significant discomfort to the patients. Herein, an injectable, biodegradable, and mechanically adaptive hydrogel loaded with platelet-rich plasma (PRP) is created by L­serine and allyl functionalized chitosan (ACS) to achieve efficient, comfortable, and minimally invasive treatment of IUAs. L­serine induces fast gelation and mechanical reinforcement of the hydrogel, while ACS introduces, imparting a good injectability and complaint yet strong feature to the hydrogel. This design enables the hydrogel to adapt to the complex geometry and match the mechanical properties of the uterine. Moreover, the hydrogel exhibits proper degradability, sustained growth factors (GFs) of PRP release ability, and good biocompatibility. Consequently, the hydrogel shows promising therapeutic efficacy by reducing collagen fiber deposition and facilitating endometrium cell proliferation, thereby restoring the fertility function of the uterus in an IUAs model of rats. Accordingly, the combination of L­serine and ACS-induced hydrogel with such advantages holds great potential for treating IUAs. STATEMENT OF SIGNIFICANCE: This research introduces a breakthrough in the treatment of intrauterine adhesions (IUAs) with an injectable, biodegradable and mechanically adaptive hydrogel using L­serine and allyl functionalized chitosan (ACS). Unlike traditional surgical treatments, this hydrogel uniquely conforms to the uterus's geometry and mechanical properties, offering a minimally invasive, comfortable, and more effective solution. The hydrogel is designed to release growth factors from platelet-rich plasma (PRP) sustainably, promoting tissue regeneration by enhancing collagen fiber deposition and endometrium cell proliferation. Demonstrated efficacy in a rat model of IUAs indicates its great potential to significantly improve fertility restoration treatments. This advancement represents a significant leap in reproductive medicine, promising to transform IUAs treatment with its innovative approach to achieving efficient, comfortable, and minimally invasive therapy.


Sujet(s)
Chitosane , Hydrogels , Plasma riche en plaquettes , Rat Sprague-Dawley , Sérine , Femelle , Animaux , Chitosane/composition chimique , Chitosane/pharmacologie , Adhérences tissulaires/anatomopathologie , Hydrogels/composition chimique , Hydrogels/pharmacologie , Sérine/composition chimique , Sérine/pharmacologie , Rats , Injections , Utérus/effets des médicaments et des substances chimiques , Utérus/anatomopathologie , Maladies de l'utérus/anatomopathologie , Maladies de l'utérus/thérapie
20.
BMC Vet Res ; 20(1): 316, 2024 Jul 16.
Article de Anglais | MEDLINE | ID: mdl-39014404

RÉSUMÉ

This study aimed to evaluate the impacts of supplemental dietary curcumin on post-partum uterine involution using pulsed-wave Doppler ultrasonography in postpartum goats. Ten pluriparous Zaraibi goats were used and divided into two groups. Group 1 (n = 5; control) received only a base diet. Group 2 (n = 5; treated) received a base diet supplemented with curcumin (200 mg/kg diet) daily for 28 days, starting from day 1 postpartum (PP) till day 28 PP. Uterine morphometrical changes (uterine horn diameter; UHD and caruncle diameter; CD), uterine hemodynamics (resistance and pulsatility indices (RI and PI), systolic/ diastolic ratio (S/D), peak systolic velocity (PSV), end-diastolic velocity (EDV), blood flow volume (BFV), and blood flow rate (BFR)), and progesterone level were evaluated. Results revealed that the diameter of the uterine horn decreased rapidly from day 1 to day 10 PP (> 50%) but more steadily from day 14 to day 28 PP in both groups. After day 21 PP, there was nearly no reduction in UHD and CD in both groups. The treated group had lower values of the RI and PI (P < 0.05) than the control group. Regarding the BFR and BFV in the treated group, there was a significant increase (P < 0.05) on day 17 PP, then started to decrease till day 28 PP. While in the control group, there was a significant decrease (P < 0.05) in BFR and BFV from day 1 PP till day 28 PP. In conclusion, the incorporation of curcumin in the diet of PP Zaraibi goats improved reproductive performance via improvements in uterine morphometric changes as well as blood perfusion.


Sujet(s)
Curcumine , Compléments alimentaires , Capra , Période du postpartum , Utérus , Animaux , Femelle , Capra/physiologie , Curcumine/pharmacologie , Curcumine/administration et posologie , Utérus/effets des médicaments et des substances chimiques , Utérus/imagerie diagnostique , Utérus/vascularisation , Période du postpartum/effets des médicaments et des substances chimiques , Régime alimentaire/médecine vétérinaire , Écho-Doppler pulsé/médecine vétérinaire , Aliment pour animaux/analyse , Progestérone/sang
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