RÉSUMÉ
Ebstein's anomaly is a congenital malformation of the tricuspid valve characterized by abnormal attachment of the valve leaflets, resulting in varying degrees of valve dysfunction. The anatomic hallmarks of this entity are the downward displacement of the attachment of the septal and posterior leaflets of the tricuspid valve. Additional intracardiac malformations are common. From an embryological point of view, the cavity of the future right atrium does not have a direct orifice connected to the developing right ventricle. This chapter provides an overview of current insight into how this connection is formed and how malformations of the tricuspid valve arise from dysregulation of molecular and morphological events involved in this process. Furthermore, mouse models that show features of Ebstein's anomaly and the naturally occurring model of canine tricuspid valve malformation are described and compared to the human model. Although Ebstein's anomaly remains one of the least understood cardiac malformations to date, the studies summarized here provide, in aggregate, evidence for monogenic and oligogenic factors driving pathogenesis.
Sujet(s)
Modèles animaux de maladie humaine , Maladie d'Ebstein , Valve atrioventriculaire droite , Maladie d'Ebstein/génétique , Maladie d'Ebstein/anatomopathologie , Maladie d'Ebstein/physiopathologie , Animaux , Humains , Chiens , Souris , Valve atrioventriculaire droite/malformations , Valve atrioventriculaire droite/anatomopathologieRÉSUMÉ
Tricuspid valve disease is an often underrecognized clinical problem that is associated with significant morbidity and mortality. Unfortunately, patients will often present late in their disease course with severe right-sided heart failure, pulmonary hypertension, and life-limiting symptoms that have few durable treatment options. Traditionally, the only treatment for tricuspid valve disease has been medical therapy or surgery; however, there have been increasing interest and success with the use of transcatheter tricuspid valve therapies over the past several years to treat patients with previously limited therapeutic options. The tricuspid valve is complex anatomically, lying adjacent to important anatomic structures such as the right coronary artery and the atrioventricular node, and is the passageway for permanent pacemaker leads into the right ventricle. In addition, the mechanism of tricuspid pathology varies widely between patients, which can be due to primary, secondary, or a combination of causes, meaning that it is not possible for 1 type of device to be suitable for treatment of all cases of tricuspid valve disease. To best visualize the pathology, several modalities of advanced cardiac imaging are often required, including transthoracic echocardiography, transesophageal echocardiography, cardiac computed tomography, and cardiac magnetic resonance imaging, to best visualize the pathology. This detailed imaging provides important information for choosing the ideal transcatheter treatment options for patients with tricuspid valve disease, taking into account the need for the lifetime management of the patient. This review highlights the important background, anatomic considerations, therapeutic options, and future directions with regard to treatment of tricuspid valve disease.
Sujet(s)
Association américaine du coeur , Valve atrioventriculaire droite , Humains , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/anatomopathologie , États-Unis , Valvulopathies/thérapie , Valvulopathies/imagerie diagnostique , Insuffisance tricuspide/imagerie diagnostique , Insuffisance tricuspide/thérapie , Implantation de valve prothétique cardiaqueRÉSUMÉ
Ebstein's anomaly, a rare congenital heart disease, is distinguished by the failure of embryological delamination of the tricuspid valve leaflets from the underlying primitive right ventricle myocardium. Gaining insight into the genetic basis of Ebstein's anomaly allows a more precise definition of its pathogenesis. In this study, two distinct cohorts from the Chinese Han population were included: a case-control cohort consisting of 82 unrelated cases and 125 controls without cardiac phenotypes and a trio cohort comprising 36 parent-offspring trios. Whole-exome sequencing data from all 315 participants were utilized to identify qualifying variants, encompassing rare (minor allele frequency < 0.1% from East Asians in the gnomAD database) functional variants and high-confidence (HC) loss-of-function (LoF) variants. Various statistical models, including burden tests and variance-component models, were employed to identify rare variants, genes, and biological pathways associated with Ebstein's anomaly. Significant associations were noted between Ebstein's anomaly and rare HC LoF variants found in genes related to the matrisome, a collection of extracellular matrix (ECM) components. Specifically, 47 genes with HC LoF variants were exclusively or predominantly identified in cases, while nine genes showed such variants in the probands. Over half of unrelated cases (n = 42) and approximately one-third of probands (n = 12) were found to carry one or two LoF variants in these prioritized genes. These results highlight the role of the matrisome in the pathogenesis of Ebstein's anomaly, contributing to a better understanding of the genetic architecture underlying this condition. Our findings hold the potential to impact the genetic diagnosis and treatment approaches for Ebstein's anomaly.
Sujet(s)
Maladie d'Ebstein , Cardiopathies congénitales , Humains , Maladie d'Ebstein/génétique , Valve atrioventriculaire droite/anatomopathologie , Cardiopathies congénitales/complications , Myocarde/anatomopathologie , Ventricules cardiaques/anatomopathologieRÉSUMÉ
Intracardiac masses adhering to the tricuspid valve can occur as a result of right-sided infective endocarditis, malignancy, clot formation in the right atrium, or clots-in-transit passing through the right atrium. Early surgical intervention is recommended for tricuspid valve vegetation in some patients, although open heart surgery is not always an option. Treatment options for right heart thrombi include anticoagulation, thrombolysis, surgical embolectomy, or mechanical aspiration. We present a case series of tricuspid valve debulking using aspiration with the FlowTriever System.
Sujet(s)
Endocardite bactérienne , Endocardite , Thrombose , Humains , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/chirurgie , Valve atrioventriculaire droite/anatomopathologie , Endocardite bactérienne/chirurgie , Interventions chirurgicales de cytoréduction , Résultat thérapeutiqueRÉSUMÉ
Pathogenic mechanisms in degenerative thoracic aortic aneurysms (TAA) are still unclear. There is an ongoing debate about whether TAAs are caused by uniform or distinct processes, which would obviously have a major impact on future treatment strategies. Clearly, the ultimate outcome of TAA subgroups associated with a tricuspid aortic valve (TAV) or a bicuspid aortic valve (BAV) is the same, namely a TAA. Based on results from our own and others' studies, we decided to compare the different TAAs (TAV and BAV) and controls using a broad array of analyses, i.e., metabolomic analyses, gene expression profiling, protein expression analyses, histological characterization, and matrix-assisted laser desorption ionization imaging. Central findings of the present study are the presence of noncanonical atherosclerosis, pathological accumulation of macrophages, and disturbances of lipid metabolism in the aortic media. Moreover, we have also found that lipid metabolism is impaired systemically. Importantly, all of the above-described phenotypes are characteristic for TAV-TAA only, and not for BAV-TAA. In summary, our results suggest different modes of pathogenesis in TAV- and BAV-associated aneurysms. Intimal atherosclerotic changes play a more central role in TAV-TAA formation than previously thought, particularly as the observed alterations do not follow classical patterns. Atherosclerotic alterations are not limited to the intima but also affect and alter the TAV-TAA media. Further studies are needed to i) clarify patho-relevant intima-media interconnections, ii) define the origin of the systemic alteration of lipid metabolism, and iii) to define valid biomarkers for early diagnosis, disease progression, and successful treatments in TAV-TAAs.
Sujet(s)
Anévrysme de l'aorte thoracique , Maladie de la valve aortique bicuspide , Valvulopathies , Humains , Valve aortique/métabolisme , Valve aortique/anatomopathologie , Valvulopathies/complications , Valvulopathies/métabolisme , Valvulopathies/anatomopathologie , Valve atrioventriculaire droite/métabolisme , Valve atrioventriculaire droite/anatomopathologie , Aorte/métabolisme , Maladie de la valve aortique bicuspide/complications , Maladie de la valve aortique bicuspide/métabolisme , Maladie de la valve aortique bicuspide/anatomopathologie , Anévrysme de l'aorte thoracique/complications , Anévrysme de l'aorte thoracique/anatomopathologieRÉSUMÉ
Stem cell niches have been thoroughly investigated in tissue with high regenerative capacity but not in tissues where cell turnover is slow, such as the human heart. The left AtrioVentricular junction (AVj), the base of the mitral valve, has previously been proposed as a niche region for cardiac progenitors in the adult human heart. In the present study, we explore the right side of the human heart, the base of the tricuspid valve, to investigate the potential of this region as a progenitor niche. Paired biopsies from explanted human hearts were collected from multi-organ donors (N = 12). The lateral side of the AVj, right atria (RA), and right ventricle (RV) were compared for the expression of stem cell niche-related biomarkers using RNA sequencing. Gene expression data indicated upregulation of genes related to embryonic development and extracellular matrix (ECM) composition in the proposed niche region, that is, the AVj. In addition, immunohistochemistry showed high expression of the fetal cardiac markers MDR1, SSEA4, and WT1 within the same region. Nuclear expression of HIF1α was detected suggesting hypoxia. Rare cells were found with the co-staining of the proliferation marker PCNA and Ki67 with cardiomyocyte nuclei marker PCM1 and cardiac Troponin T (cTnT), indicating proliferation of small cardiomyocytes. WT1+/cTnT+ and SSEA4+/cTnT+ cells were also found, suggesting cardiomyocyte-specific progenitors. The expression of the stem cell markers gradually decreased with distance from the tricuspid valve. No expression of these markers was observed in the RV tissue. In summary, the base of the tricuspid valve is an ECM-rich region containing cells with expression of several stem cell niche-associated markers. Co-expression of stem cell markers with cTnT indicates cardiomyocyte-specific progenitors. We previously reported similar data from the base of the mitral valve and thus propose that human adult cardiomyocyte progenitors reside around both atrioventricular valves.
Sujet(s)
Niche de cellules souches , Valve atrioventriculaire droite , Adulte , Humains , Valve atrioventriculaire droite/anatomopathologie , Myocytes cardiaques/métabolisme , Ventricules cardiaques , Marqueurs biologiques/métabolismeRÉSUMÉ
BACKGROUND AND AIM: Papillary fibroelastoma (PFE) represents only 16% of the benign cardiac tumor and approximately 15% of these are located on the tricuspid valve. MATERIALS AND METHODS: Over a period of 22 years (1999-2021) we observed 75 pts with cardiac tumors at our Center over 9650 pts operated on but only one case of a tricuspid valve PFE in a 69-year-old patient. Trans-thoracic echocardiography demonstrated a mobile mass (20 × 10 mm), adhering to the atrial side of the septal leaflet of the tricuspid valve of unknown origin. In consideration of the mobility of the mass and the consequent high embolic risk, surgical removal was made. The patient underwent surgery through a median sternotomy on CPBP. A 'gelatinous' mass adhering to the tricuspid leaflet was found and completely removed. The postoperative course was uneventful. The pathological diagnosis was PFE. CONCLUSIONS: PFEs of the tricuspid valve are rare entities being in most cases found incidentally. In our experience, the incidence of this tumor in this location is 1/10,000 cases of cardiac surgery. Although most patients are asymptomatic, surgical treatment is nevertheless recommended in consideration of the high embolic risk.
Sujet(s)
Fibroélastome papillaire cardiaque , Fibrome , Tumeurs du coeur , Humains , Sujet âgé , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/chirurgie , Valve atrioventriculaire droite/anatomopathologie , Fibroélastome papillaire cardiaque/anatomopathologie , Fibrome/imagerie diagnostique , Fibrome/chirurgie , Échocardiographie , Tumeurs du coeur/imagerie diagnostique , Tumeurs du coeur/chirurgieRÉSUMÉ
OBJECTIVES: We aimed to evaluate immediate and midterm cardiac remodeling after surgery by cardiac magnetic resonance (CMR) in Ebstein's anomaly (EA), and also to investigate preoperative predictors of right ventricular (RV) normalization. METHODS: We retrospectively analyzed CMR parameters of the whole heart in adult patients with EA before surgery, at discharge and follow-up. RESULTS: A total of 26 patients were included and performed CMR at 7 days (interquartile range, 3-13 days) before surgery. Immediate postoperative CMR was finished at discharge (median: 8 [7-9] days; n = 18) and follow-up CMR at 187 days (interquartile range, 167-356 days; n = 17). RV and right atrial (RA) volumes promptly decreased immediately after surgery and at follow-up (all p < 0.05). RV ejection fraction decreased significantly at discharge (p < 0.05) but recovered at follow-up (p = 0.18). However, RV global longitudinal strain and RA reservoir strain were significantly impaired immediately and midterm after surgery (all p < 0.05). Indexed left ventricular (LV) end-diastolic volume, stroke volume, as well as global longitudinal strain increased from preoperative to follow-up (all p < 0.05). Patients who achieved normalization of RV volumes after surgery had smaller severity index and RV and RA volumes and higher LV ejection fraction and RA reservoir strain at baseline than patients without RV normalization (all p < 0.05). CONCLUSIONS: Reverse biventricular remodeling took place in EA after tricuspid valve surgery. Tricuspid valve reconstruction should be performed before deterioration of RV volume overload and LV function to achieve reverse RV remodeling. Key Points ⢠After removing the volume load of tricuspid regurgitation in Ebstein's anomaly, reverse remodeling was detected by CMR in both left and right heart at midterm follow-up. ⢠Tricuspid valve reconstruction should be performed before deterioration of RV volume overload and LV function to achieve reverse RV remodeling.
Sujet(s)
Maladie d'Ebstein , Défaillance cardiaque , Adulte , Humains , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/chirurgie , Valve atrioventriculaire droite/anatomopathologie , Maladie d'Ebstein/imagerie diagnostique , Maladie d'Ebstein/chirurgie , Maladie d'Ebstein/anatomopathologie , Études rétrospectives , Remodelage ventriculaire , Fonction ventriculaire droite , Spectroscopie par résonance magnétiqueRÉSUMÉ
Marantic endocarditis refers to a noninfectious lesion, usually in the aortic and mitral valves, that is most commonly seen in advanced malignancy and systemic lupus erythematosus. Inflammatory conditions, including antiphospholipid syndrome (APS), are a rare etiology making up less than 20% of reported cases. The condition is thought to be due to a hypercoagulable state and found postmortem with rates in autopsy series ranging from 0.9% to 1.6%. In comparison to infective endocarditis, marantic endocarditis has a greater tendency for valve vegetations to embolize. Common treatment modalities include anticoagulation or valve replacement. Although percutaneous aspiration thrombectomy of right-sided heart chamber thrombi exists, there are limited reports demonstrating its use with regards to treatment of right-sided endocarditis. We present the case of an older male with a history of Factor V Leiden and APS who was admitted due to a rapidly expanding mass on the tricuspid valve (TV). Despite serial blood cultures being negative, the patient received adequate antibiotic therapy for more than 4 weeks. Transthoracic echocardiogram showed an enlarged TV vegetation with an increased diameter from 10 to 30 mm over 6 weeks. Due to the patient's high operative risk and concern for embolization complications, a multidisciplinary decision was made to perform percutaneous aspiration thrombectomy of the TV vegetation. Subsequent biopsy of the lesion confirmed it was noninfectious and nonmalignant. Thus, the patient was started on systemic anticoagulation for prevention of thromboembolic events.
Sujet(s)
Syndrome des anticorps antiphospholipides , Endocardite , Mâle , Humains , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/chirurgie , Valve atrioventriculaire droite/anatomopathologie , Résultat thérapeutique , Endocardite/complications , Syndrome des anticorps antiphospholipides/complications , Thrombectomie/effets indésirables , Anticoagulants/usage thérapeutiqueRÉSUMÉ
BACKGROUND: Hemostatic disturbances with coronavirus disease 2019 (COVID-19) can predispose to tricuspid and right heart thrombi in very rare instances. AIM: We describe a 29-year-old female patient without a previous cause of thrombosis who developed large tricuspid valve thrombus (TVT) and moderate-to-severe tricuspid regurgitation (TR) during the course of COVID-19 infection. MATERIALS AND METHODS: Persistant fever and tachycardia with thrombocytopenia and high d-dimer increased the index of suspicion. The diagnosis was made by bedside transthoracic echocardiography (TTE) and cardiac magnetic resonance (CMR). Surgery was performed for thrombectomy and tricuspid valve replacement with a tissue valve. DISCUSSION AND CONCLUSION: Detection of TVT in COVID-19 patients on the basis of high index of suspicion, bedside TTE and noninvasive CMR helps early surgical treatment and subsequent reduction of mortality and hospital stay.
Sujet(s)
COVID-19 , Implantation de valve prothétique cardiaque , Thrombose , Insuffisance tricuspide , Adulte , COVID-19/complications , Échocardiographie , Femelle , Implantation de valve prothétique cardiaque/effets indésirables , Humains , Thrombose/imagerie diagnostique , Thrombose/étiologie , Thrombose/chirurgie , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/anatomopathologie , Valve atrioventriculaire droite/chirurgie , Insuffisance tricuspide/imagerie diagnostique , Insuffisance tricuspide/étiologie , Insuffisance tricuspide/chirurgieRÉSUMÉ
Tricuspid pouch forms during the spontaneous closure of a ventricular septal defect (VSD). Cases have been reported in which the tricuspid pouch was discovered for the first time during surgery and could not be distinguished from an aneurysm of the membranous septum( AMS). A 58-year-old woman had a heart murmur. Transthoracic echocardiography showed an aneurysm-like pouch protruding into the right ventricle. Magnetic resonance imaging could not distinguish between AMS and tricuspid pouch;however, contrast-enhanced computed tomography showed a VSD. The membranous structure comprised multiple lobules, and the tendon of the papillary muscles was continuous with the tricuspid valve. Intraoperatively, the tricuspid valve septal leaflet was adhered to the defect hole. It was incised along the annulus, the VSD was closed with a bovine pericardial patch, and the annulus of the tricuspid valve septal leaflet was suture closed. The patient was discharged after a good postoperative course.
Sujet(s)
Défaillance cardiaque , Communications interventriculaires , Animaux , Bovins , Échocardiographie , Femelle , Défaillance cardiaque/imagerie diagnostique , Défaillance cardiaque/étiologie , Défaillance cardiaque/chirurgie , Communications interventriculaires/chirurgie , Ventricules cardiaques , Humains , Adulte d'âge moyen , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/anatomopathologie , Valve atrioventriculaire droite/chirurgieRÉSUMÉ
A 67-year-old woman presented with dyspnea on effort and cyanosis due to massive tricuspid regurgitation and an atrial septal defect with right to left shunt. She was diagnosed with Ebstein disease at the age of 53 when she underwent surgery for varicose veins. Echocardiography showed the severe apical displacement of the septal and posterior leaflet. The anterior leaflet also partially displaced to the apex and demonstrated tethering caused by a dilated right ventricle. Cardiac magnetic resonance imaging showed a dilated right atrium and an enlarged atrialized right ventricle, in addition to marked low cardiac output in the dilated right ventricle. The surgical findings corresponded to Carpentier classification type C. Cone reconstruction was performed. Bidirectional Glenn anastomosis was reguired because of low cardiac output in the remaining functional right ventricle after Cone reconstruction. The patient's postoperative course was uneventful, and tricuspid regurgitation and stenosis remained mild. The patients had no occurrence of right heart failure or arrhythmia for two years after surgery.
Sujet(s)
Procédures de chirurgie cardiaque , Maladie d'Ebstein , Adulte , Sujet âgé , Procédures de chirurgie cardiaque/méthodes , Maladie d'Ebstein/complications , Maladie d'Ebstein/imagerie diagnostique , Maladie d'Ebstein/chirurgie , Femelle , Ventricules cardiaques/imagerie diagnostique , Ventricules cardiaques/chirurgie , Humains , Résultat thérapeutique , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/anatomopathologie , Valve atrioventriculaire droite/chirurgieRÉSUMÉ
Background and Objectives: The aim of this study was to clarify the tricuspid valve (TV) and right ventricular (RV) geometry and function characteristics using 3D echocardiography-based analysis and to identify echocardiographic predictors for severe tricuspid regurgitation (TR) in different etiologies of functional TR (fTR). Methods and Results: The prospective study included 128 patients (median age 64 years, 57% females): 109 patients with moderate or severe fTR (69-caused by dominant left-sided valvular pathology (LSVP), 40 due to precapillary pulmonary hypertension (PH)), and 19 healthy controls. The 2D and 3D-transthoracic echocardiography analysis included TV, right atrium, RV geometry, and functional parameters. All the RV geometry parameters as well as 3D TV parameters were increased in both fTR groups when compared to controls. Higher RV diameters, length, areas, volumes, and more impaired RV function were in PH group compared to LSVP group. PH was associated with larger leaflet tenting height, volume, and more increased indices of septal-lateral and major axis tricuspid annulus (TA) diameters. LVSP etiology was associated with higher anterior-posterior TA diameter and sphericity index. Univariate and multivariate logistic regression and ROC analyses revealed that different fTR etiologies were associated with various 2D and 3D echocardiographic parameters to predict severe TR: major axis TA diameter and TA perimeter, the leaflet tenting volume had the highest predictive value in PH group, septal-lateral systolic TA diameter-in LSVP group. The 3D TA analysis provided more reliable prediction for severe fTR. Conclusions: TV and RV geometry vary in different etiologies of functional TR. Precapillary PH is related to more severe RV remodeling and dysfunction and changes of TV geometry, when compared to LSVP group. The 3D echocardiography helps to determine echocardiographic predictors of severe TR in different fTR etiologies.
Sujet(s)
Échocardiographie tridimensionnelle , Hypertension pulmonaire , Insuffisance tricuspide , Femelle , Humains , Adulte d'âge moyen , Mâle , Insuffisance tricuspide/imagerie diagnostique , Insuffisance tricuspide/étiologie , Insuffisance tricuspide/anatomopathologie , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/anatomopathologie , Études prospectives , Échocardiographie , Hypertension pulmonaire/complications , Hypertension pulmonaire/imagerie diagnostiqueRÉSUMÉ
BACKGROUND: Tricuspid regurgitation is common in patients with severe degenerative mitral regurgitation. However, the evidence base is insufficient to inform a decision about whether to perform tricuspid-valve repair during mitral-valve surgery in patients who have moderate tricuspid regurgitation or less-than-moderate regurgitation with annular dilatation. METHODS: We randomly assigned 401 patients who were undergoing mitral-valve surgery for degenerative mitral regurgitation to receive a procedure with or without tricuspid annuloplasty (TA). The primary 2-year end point was a composite of reoperation for tricuspid regurgitation, progression of tricuspid regurgitation by two grades from baseline or the presence of severe tricuspid regurgitation, or death. RESULTS: Patients who underwent mitral-valve surgery plus TA had fewer primary-end-point events than those who underwent mitral-valve surgery alone (3.9% vs. 10.2%) (relative risk, 0.37; 95% confidence interval [CI], 0.16 to 0.86; P = 0.02). Two-year mortality was 3.2% in the surgery-plus-TA group and 4.5% in the surgery-alone group (relative risk, 0.69; 95% CI, 0.25 to 1.88). The 2-year prevalence of progression of tricuspid regurgitation was lower in the surgery-plus-TA group than in the surgery-alone group (0.6% vs. 6.1%; relative risk, 0.09; 95% CI, 0.01 to 0.69). The frequencies of major adverse cardiac and cerebrovascular events, functional status, and quality of life were similar in the two groups at 2 years, although the incidence of permanent pacemaker implantation was higher in the surgery-plus-TA group than in the surgery-alone group (14.1% vs. 2.5%; rate ratio, 5.75; 95% CI, 2.27 to 14.60). CONCLUSIONS: Among patients undergoing mitral-valve surgery, those who also received TA had a lower incidence of a primary-end-point event than those who underwent mitral-valve surgery alone at 2 years, a reduction that was driven by less frequent progression to severe tricuspid regurgitation. Tricuspid repair resulted in more frequent permanent pacemaker implantation. Whether reduced progression of tricuspid regurgitation results in long-term clinical benefit can be determined only with longer follow-up. (Funded by the National Heart, Lung, and Blood Institute and the German Center for Cardiovascular Research; ClinicalTrials.gov number, NCT02675244.).
Sujet(s)
Annuloplastie de valves cardiaques , Évolution de la maladie , Insuffisance mitrale/chirurgie , Insuffisance tricuspide/chirurgie , Valve atrioventriculaire droite/chirurgie , Sujet âgé , Dilatation pathologique , Femelle , Études de suivi , Humains , Analyse en intention de traitement , Mâle , Valve atrioventriculaire gauche/chirurgie , Insuffisance mitrale/complications , Insuffisance mitrale/mortalité , Pacemaker , Complications postopératoires , Qualité de vie , Réintervention , Analyse de survie , Valve atrioventriculaire droite/anatomopathologie , Insuffisance tricuspide/complications , Insuffisance tricuspide/thérapieRÉSUMÉ
Calcification of the bicuspid aortic valve (BAV) involves differential expression of various RNA genes, which is achieved through complex regulatory networks that are controlled in part by transcription factors and microRNAs. We previously found that miR-195-5p regulates the osteogenic differentiation of valvular interstitial cells (VICs) by targeting the TGF-ß pathway. However, the transcriptional regulation of miR-195-5p in calcified BAV patients is not yet clear. In this study, stenotic aortic valve tissues from patients with BAVs and tricuspid aortic valves (TAVs) were collected. Candidate transcription factors of miR-195-5p were predicted by bioinformatics analysis and tested in diseased valves and in male porcine VICs. SP2 gene expression and the corresponding protein levels in BAV were significantly lower than those in TAV, and a low SP2 expression level environment in VICs resulted in remarkable increases in RNA expression levels of RUNX2, BMP2, collagen 1, MMP2, and MMP9 and the corresponding proteins. ChIP assays revealed that SP2 directly bound to the transcription promoter region of miR-195-5p. Cotransfection of SP2 shRNA and a miR-195-5p mimic in porcine VICs demonstrated that SP2 repressed SMAD7 expression via miR-195-5p, while knockdown of SP2 increased the mRNA expression of SMAD7 and the corresponding protein and attenuated Smad 2/3 expression. Immunofluorescence staining of diseased valves confirmed that the functional proteins of osteogenesis differentiation, including RUNX2, BMP2, collagen 1, and osteocalcin, were overexpressed in BAVs. In Conclusion, the transcription factor Sp2 is expressed at low levels in VICs from BAV patients, which has a negative impact on miR-195-5p expression by binding its promoter region and partially promotes calcification through a SMAD-dependent pathway.
Sujet(s)
Maladie de la valve aortique bicuspide/anatomopathologie , Calcinose/anatomopathologie , Ostéoblastes/anatomopathologie , Protéine Smad7/métabolisme , Facteur de transcription Sp2/métabolisme , Facteur de croissance transformant bêta-1/métabolisme , Valve atrioventriculaire droite/anatomopathologie , Animaux , Maladie de la valve aortique bicuspide/génétique , Maladie de la valve aortique bicuspide/métabolisme , Protéine morphogénétique osseuse de type 2/génétique , Protéine morphogénétique osseuse de type 2/métabolisme , Calcinose/génétique , Calcinose/métabolisme , Différenciation cellulaire , Sous-unité alpha 1 du facteur CBF/génétique , Sous-unité alpha 1 du facteur CBF/métabolisme , Femelle , Humains , Mâle , microARN , Adulte d'âge moyen , Ostéoblastes/métabolisme , Ostéogenèse , Protéine Smad7/génétique , Facteur de transcription Sp2/génétique , Suidae , Facteur de croissance transformant bêta-1/génétique , Valve atrioventriculaire droite/métabolismeRÉSUMÉ
BACKGROUND: Leadless pacemakers (LPs) have proven safe and effective, but device revisions remain necessary. Either replacing the LP or implanting a new adjacent LP is feasible. Replacement seems more appealing, but encapsulation and tissue adhesions may hamper the safety and efficacy of LP retrieval. OBJECTIVE: We determined the incidence and cellular characteristics of tissue adherent to retrieved LPs and the potential implications for end-of-life strategy. METHODS: All 15 consecutive successful Nanostim LP retrievals in a tertiary center were included. We assessed the histopathology of adherent tissue and obtained clinical characteristics. RESULTS: Adherent tissue was present in 14 of 15 retrievals (93%; median implantation duration 36 months; range 0-96 months). The tissue consisted of fibrosis (n = 2), fibrosis and thrombus (n = 9), or thrombus only (n = 3). In short-term retrievals (<1 year), mostly fresh thrombi without fibrosis were seen. In later retrievals, the tissue consisted of fibrosis often with organizing or lytic thrombi. Fibrosis showed different stages of organization, notably early fibrocellular and later fibrosclerotic tissue. Inflammatory cells were seen (n = 4) without signs of infection. Tricuspid valve material was retrieved in 1 patient after 36 months, resulting in increased tricuspid regurgitation. CONCLUSION: Our results suggest that fibrosis and thrombus adherent to LPs are common and encapsulate the LP as seen in transvenous pacemakers. LPs may adhere to the tricuspid valve or subvalvular apparatus affecting retrieval safety. The end-of-life strategy should be optimized by incorporating risk stratification for excessive fibrotic encapsulation and adhesions.
Sujet(s)
Ablation de dispositif/méthodes , Effets indésirables à long terme/anatomopathologie , Pacemaker , Réintervention , Adhérences tissulaires , Valve atrioventriculaire droite , Sujet âgé de 80 ans ou plus , Bradycardie/thérapie , Analyse de panne d'appareillage , Femelle , Techniques histologiques , Humains , Mâle , , Pacemaker/effets indésirables , Pacemaker/statistiques et données numériques , Implantation de prothèse/effets indésirables , Implantation de prothèse/instrumentation , Implantation de prothèse/méthodes , Réintervention/effets indésirables , Réintervention/instrumentation , Réintervention/méthodes , Adhérences tissulaires/étiologie , Adhérences tissulaires/anatomopathologie , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/anatomopathologie , Valve atrioventriculaire droite/physiopathologieSujet(s)
Procédures de chirurgie cardiaque/méthodes , Électrocardiographie/méthodes , Endocardite bactérienne , Valve atrioventriculaire droite , Sondes cardiaques , Endocardite bactérienne/diagnostic , Endocardite bactérienne/physiopathologie , Endocardite bactérienne/chirurgie , Humains , Sélection de patients , Indice de gravité de la maladie , Toxicomanie intraveineuse , Valve atrioventriculaire droite/imagerie diagnostique , Valve atrioventriculaire droite/anatomopathologieRÉSUMÉ
A 75-year-old was treated for prostate adenocarcinoma with brachytherapy in September 2018. A routine follow-up chest radiograph 3 months later revealed a metallic object of the same dimensions as a brachytherapy pellet located in the right ventricle. Further imaging showed the brachtherapy pellet was located in the anterobasal right ventricular endocardium close to the tricuspid valve. Frequent asymptomatic premature ventricular contractions were observed with likely origin from the left ventricular outflow tract, an area remote from the site of the pellet. The patient remains asymptomatic and subsequent imaging shows that the position of the pellet has not changed.