ABSTRACT
Background: We studied the potential of human bone marrow-derived mesenchymal stem cell conditioned media (hBMSC CM) in protecting endothelial cell properties (viability, proliferation, and migrations) from the deleterious effects produced by the inflammatory environment of H2O2. Additionally, we investigated their impact on the endothelial cells' gene expression of some inflammatory-related genes, namely, TGF-ß1, FOS, ATF3, RAF-1, and SMAD3. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured individually under three conditions: alone, with varying concentrations of H2O2, or with varying concentrations of H2O2 and hBMSC CM. HUVEC adhesion, proliferation, and migration were evaluated using the xCELLigence system. The HUVECs' gene expressions were evaluated by real-time polymerase chain reaction (RT-PCR). Results: Generally, we observed enhanced HUVEC viability, proliferation, and migration when cultured in media supplemented with H2O2 and hBMSC CM. Furthermore, the CM modulated the expressions of the studied inflammatory-related genes in HUVECs, promoting a more robust cellular response. Conclusion: This study has illuminated the protective role of hBMSC CM in mitigating the damaging effects of H2O2 on endothelial cell function. Our data demonstrate that hBMSC CM enhances the viability, proliferation, and migration of HUVECs even under oxidative stress conditions. Additionally, the conditioned medium was found to modulate the gene expression of pivotal markers related to inflammation, suggesting a favorable influence on cellular response mechanisms.
Subject(s)
Atherosclerosis , Cell Movement , Cell Proliferation , Human Umbilical Vein Endothelial Cells , Hydrogen Peroxide , Mesenchymal Stem Cells , Humans , Hydrogen Peroxide/pharmacology , Hydrogen Peroxide/toxicity , Culture Media, Conditioned/pharmacology , Human Umbilical Vein Endothelial Cells/drug effects , Human Umbilical Vein Endothelial Cells/metabolism , Mesenchymal Stem Cells/metabolism , Mesenchymal Stem Cells/drug effects , Cell Proliferation/drug effects , Atherosclerosis/genetics , Atherosclerosis/metabolism , Cell Movement/drug effects , Cell Survival/drug effects , Gene Expression Regulation/drug effectsABSTRACT
Objectives The aim of the study is to measure the prevalence of suicidal ideation among nurses at King Saud University Medical City, compare its prevalence between male and female nurses, and identify the potential risk factors. Methods We conducted a cross-sectional study. The questionnaire was distributed to nurses via email. It consisted of demographics, Depression, Anxiety and Stress Scale (DASS21), and Suicidal Ideation Scale (SIS). We used the Statistical Package for the Social Sciences (SPSS) statistical software for analysis. Results The total number of participants was 419. The estimated prevalence of suicidal ideation among nurses was 24.58%. The prevalence among female and male nurses was 24.67% and 23.68%, respectively. Moreover, we found that nurses who are non-Muslim, single, and living by themselves are highly correlated with suicidal ideation. Depression, stress, and anxiety are also significantly associated with suicidality, with depression being the most significantly related to suicidal ideation. Conclusion Nurses who experienced depression, anxiety, and stress had an increased likelihood of suicidal ideation. This study demonstrates the need to raise awareness of depression, anxiety, and stress in order to prevent suicidal ideation among nurses. Further research is needed to develop measures of successful monitoring and prevention.
ABSTRACT
SUMMARY: The current report presented a rare variant of extensor indicis brevis muscle, replacing extensor indicis, in the left hand of an adult male cadaver. The origin of the muscle was reported, for the first time, to be from the distal margins of radius and ulna. The muscle is inserted into the extensor expansion of the index. A new classification for extensor indicis brevis muscle was proposed based on its origin. Awareness of rare anatomical variations would help clinicians and surgeons in accurately managing suspected cases and planning surgical procedures.
RESUMEN: Este informe presenta una variante rara del músculo extensor corto del índice, que reemplaza al extensor del índice en la mano izquierda de un cadáver masculino adulto. Se informó por primera vez, que el origen del músculo se realiza en la parte distal de los márgenes del radio y la ulna. El músculo se insertaba en la expansión extensora del índice. Se propuso una nueva clasificación para el músculo extensor corto del índice basada en su origen. El conocimiento de las variaciones anatómicas raras es útil para los médicos y cirujanos al abordar los casos sospechosos y planificar los procedimientos quirúrgicos.