ABSTRACT
AIM: Genotype characteristic and determination of serological properties of Vibrio cholerae nonO1/nonO139 strains that caused diseases in population of Rostov region from 2000 to 2009. MATERIALS AND METHODS: 15 clinical strains of V. cholerae nonO1/nonO139 were studied. Serotyping was performed by using a kit of monospecific typing sera against serogroup 02-084 cholera vibrios obtained from Rostov Research Institute for Plague Control, PCR and VNTR-genotyping--by using specific primers described in scientific publications and constructed by us. RESULTS: Serologic features of strains are very diverse and strains contain various combination of pathogenicity factor genes that seem to be interchangeable. Similar pattern was observed for VNTR-genotyping. Distribution of the examined strains by VNTR-genotyping did not correlate with either PCR-genotyping data or serotyping, or place and time of isolation. CONCLUSION: The data obtained indicates a lack of general source of human infection even in the same location and time period. On the other hand, serological and genotypic features of V. cholerae nonO1/nonO139 may undergo changes in the process of staying in the macro organism or environment due to high plasticity of their genome.
Subject(s)
Cholera/epidemiology , Cholera/microbiology , Vibrio cholerae O139/classification , Vibrio cholerae non-O1/classification , Cholera Toxin/genetics , Disease Outbreaks , Genes, Bacterial , Genotype , Humans , Minisatellite Repeats/genetics , Russia/epidemiology , Serotyping , Vibrio cholerae O139/genetics , Vibrio cholerae O139/isolation & purification , Vibrio cholerae non-O1/genetics , Vibrio cholerae non-O1/isolation & purification , Virulence Factors/geneticsABSTRACT
Lysogeny was studied in Vibrio mimicus; the indicator V. cholerae El Tor strain was selected to identify phages. New V. mimicus phages were obtained and identified, which had a morphological similarity and an antigen affinity for morphological group I cholerae phages. Phage differentiation revealed that morphological group I V. mimicus phages showed certain differences manifested as their lytic activity against V. cholerae strain 1322-69 of serovar 37 while this property was absent in cholerae phages.
Subject(s)
Bacteriophages/genetics , Bacteriophages/isolation & purification , Lysogeny , Vibrio mimicus/genetics , Vibrio mimicus/virology , Cholera/microbiology , Humans , Vibrio cholerae/virologyABSTRACT
AIM: Determination of serogroup and PCR-genotyping of Vibrio cholerae non-O1/non-O139 strains isolated from surface basins and sewages of Rostov-on-Don city in 2003 - 2008. MATERIALS AND METHODS: Seven hundred strains of V. cholerae non-O1/non-O139 serogroups were studied in reaction of slide-agglutination with array of 80 diagnostic sera for non-O1/non-O139 serogroups. Selective screening of strains representing dominating serogroups was performed for extended number of genetic determinants of pathogenicity factors. RESULTS: It was established that V. cholerae belonging to serogroups O53, O67, O75, and O76 are dominating in water ecosystems of Rostov-on-Don city at this time. All studied strains were characterized by lack of cholera toxin genes and toxin-coregulated pili but had different combinations of genes of additional virulence factors. There was no correlation between genotypic characteristics and serogroup. CONCLUSION: The study showed that change of serologic landscape of V. cholerae non-O1/non-O139 occurred in water objects in studied area during last decades. Necessity of dynamic surveillance for circulation of V. cholerae non-O1/non-O139 in aquatic environment with widening of studied spectrum of their biological features was demonstrated.
Subject(s)
Cholera/virology , Environmental Monitoring , Vibrio cholerae non-O1/classification , Vibrio cholerae/classification , Water Microbiology , Cholera/epidemiology , Cholera Toxin/genetics , Epidemiological Monitoring , Fimbriae, Bacterial/genetics , Genes, Bacterial , Humans , Russia/epidemiology , Serotyping , Vibrio cholerae/genetics , Vibrio cholerae/isolation & purification , Vibrio cholerae O139/classification , Vibrio cholerae O139/genetics , Vibrio cholerae O139/isolation & purification , Vibrio cholerae non-O1/genetics , Vibrio cholerae non-O1/isolation & purification , Virulence Factors/geneticsABSTRACT
The agglutinating properties of MCA-O1 of the IgG class and MCA-O139 of the IgM class towards epitopes of O-antigen of Vibrio cholerae O1 and accordingly Vibrio cholerae O139 were studied. The ascitic and cultural fluids by hybridomas F8G12 and D11 deposited in the specialized Collection of Cell Cultures of Vertebrates (Saint Petersburg) under RKKK (II) 386 D and RKKK (II) 674 D were the sources of monoclonal immunoglobulins. The advantage of diagnostic monoclonal immunoglobulins is that they are distinguished for strict specificity and their use in practical health care contributes to the higher specificity of a laboratory test for cholera and to its shorter performance.
Subject(s)
Antibodies, Monoclonal , Antigens, Bacterial/immunology , Vibrio cholerae/classification , Agglutination Tests , Epitopes , Sensitivity and Specificity , Vibrio cholerae/immunologyABSTRACT
The results of the serotyping of 244 V. cholerae non O1/O139 cultures isolated from patients in Uzbekistan in 2000 and 2001 are presented. All isolates were studied by the method of molecular probing and in the polymerase chain reaction for the presence of virulence genes and for sensitivity to phages ctx+, ctx- and hemolytic activity. The use of monoreceptor O-sera O2-O83 made it possible to determine vibrios of 32 serogroups with the dominating role in the etiology of acute enteric diseases belonging to serogroups O18, O62, O82, O37. Genes ctx AB were detected in none of the isolates, 5 of them contained gene tcp A. A group of cultures, sensitive to phage ctx+ and belonging mainly to enteropathogenic serogroups, was detected.
Subject(s)
Vibrio Infections/epidemiology , Vibrio cholerae non-O1/classification , Bacteriophages/genetics , Fimbriae Proteins/genetics , Genes, Bacterial , Humans , Molecular Epidemiology , Serotyping , Uzbekistan/epidemiology , Vibrio cholerae non-O1/genetics , Vibrio cholerae non-O1/pathogenicity , Virulence/geneticsABSTRACT
The dynamics of the isolation of V. cholerae cultures from various water objects on the territory of Rostov-on-Don during the period of 1994-2001 was analyzed and biological properties of 14 such cultures were studied. In the absence of epidemic complications during the above-mentioned period, a growth in the amount of V. cholerae isolates, serogroups 01 and 0139, including toxigenic V. cholerae 01, was registered. The microbiological and epidemiological aspects of the monitoring of surface reservoirs and sewage were considered and the expediency of the profound and systematic study of its results for epidemiological surveillance on cholera was emphasized.
Subject(s)
Sewage/microbiology , Vibrio cholerae/isolation & purification , Cholera/diagnosis , Cholera/epidemiology , Humans , Population Surveillance , Retrospective Studies , Russia/epidemiology , Serotyping , Vibrio cholerae/classification , Vibrio cholerae/physiology , Water MicrobiologyABSTRACT
The development of a method for serological identification of toxigenic and nontoxigenic V. cholerae non-O1, as well as on their role in human pathology, is reviewed. The evaluation on this method when used for establishing the etiology of acute diarrhea cases and analysis of sporadic diseases and group outbreaks of alimentary toxicoinfection type. Different points of view on the formation of toxigenic clones of V. cholerae non-O1 in nature is considered. The necessity for further development of the serological typing method is substantiated and the tasks of its improvement are set.