[124I]CLR1404 PET/CT in High-Grade Primary and Metastatic Brain Tumors.

PURPOSE: There is a continuous search for imaging techniques with high sensitivity and specificity for brain tumors. Positron emission tomography (PET) imaging has shown promise, though many PET agents either have a low tumor specificity or impractical physical half-lives. [124I]CLR1404 is a small molecule alkylphosphocholine analogue that is thought to bind to plasma membrane lipid rafts and has shown high tumor-to-background ratios (TBR) in a previous pilot study in brain tumor patients. This study attempts to define the clinical value of [124I]CLR1404 PET/CT (aka CLR124). PROCEDURES: Adult patients with new or suspected recurrence of high-grade primary or metastatic brain tumors (N = 27) were injected with [124I]CLR1404 followed by PET/CT at 6, 24, and 48 h. Standard uptake values (SUV) and TBR values were calculated for all time points. Uptake of [124I]CLR1404 was qualitatively assessed, compared with magnetic resonance imaging (MRI), and correlated with clinical outcome. Final diagnosis (N = 25) was established based on surgically resected tissue or long-term follow-up. RESULTS: Positive uptake with high TBR was detected in all but one patient with a final diagnosis of primary/recurrent brain tumor (12/13) and in less than half of patients with treatment-related changes (5/12). Concordance between [124I]CLR1404 uptake and contrast enhancement on MRI was seen in < 40 %, with no concordance between T2-hyperintensities and uptake. No significant difference in overall outcome was found between patients with and without [124I]CLR1404 uptake. CONCLUSIONS: The uptake pattern in these patients suggests a very high sensitivity of [124I]CLR1404 PET/CT for diagnosing tumor tissue; however, tumor specificity needs to be further defined. Relative lack of concordance with standard MRI characteristics suggests that [124I]CLR1404 PET/CT provides additional information about brain tumors compared to MRI alone, potentially improving clinical decision-making.

Endoscope-assisted retrosigmoid resection of a medium size vestibular schwannoma tumor model: a cadaveric study.

OBJECTIVE: To demonstrate a flexible endoscope assisted technique to perform microsurgical resection using a retrosigmoid approach of an artificial polymer tumor model that mimics a medium size (15-20mm diameter) vestibular schwannoma. METHODS: Twelve bilateral retrosigmoid dissections were performed in 6 glutaraldehyde embalmed, colored silicone injected, adult cadaveric heads. Using a standard retrosigmoid approach, we first implanted the tumor model at the cerebellopontine angle (CPA) and then we resected the tumor under simultaneous endoscopic and microscopic visualizations. The resection was performed by first creating a corridor by removing the lower portion of the tumor and then by inserting through the same corridor the flexible endoscope mounted on a surgical instrument in order to accomplish early visualization of the VII-VIII complex. This early visualization of the VII-VIII complex made possible expeditious removal of the model with preservation of the VII-VIII complex. RESULTS: We were able to successfully implant the artificial tumor in all the specimens. The post-tumor implantation CT scan confirmed the optimal CPA location of the model with its intra-porus extension. The exposure of the facial and the adjoining neuro-vascular structures was excellent during all stages of the surgical removal and was accomplished with minimal cerebellar retraction, under intermittent endoscopic-assisted control. Early visualization of the facial and vestibular cochlear nerves complex led to unhindered removal of the tumor model. CONCLUSIONS: The endoscopic-assisted microsurgical removal of a tumor model simulating a medium size vestibular schwannoma was feasible in our tumor model study emulating real surgery. Visualization of the acousticofacial bundle at the early stage of the surgical removal should theoretically decrease the risk of its inadvertent injuries as well as facilitate complete removal of the tumor. Clinical studies to validate this laboratory study are necessary.

An endoscopic, cadaveric analysis of the roof of the fourth ventricle.

We performed endoscopic dissections of the roof of the fourth ventricle in eight fresh human cadaveric heads to characterize the endoscopic anatomy of the roof of the fourth ventricle and the anatomical configuration of the structures forming its roof. We also made three-dimensional (3D) silicone casts of the fourth ventricle in seven formalin-fixed specimens to evaluate the 3D configuration of the structures that create the roof of the fourth ventricle. The roof of the fourth ventricle can be divided into three zones. The upper zone is formed by the superior cerebellar peduncle and superior medullary velum and is associated with the lingula. The middle zone is formed by the inferior cerebellar peduncles and inferior medullary velum and is associated with the nodule in the midline and with the peduncle of the flocculus. The lower zone is formed by the tela choroidea and is associated with the tonsils. The 3D shape of the roof the fourth ventricle resembles that of a rhomboid-based pyramid; the edges of the base represent the borders of the ventricle, and the apex is the cerebellar fastigium. The lateral recess is shaped like a triangular-based pyramid, with its base connected to the cavity of the fourth ventricle and its tip opening into the lateral cerebellomedullary cistern through the foramen of Luschka. Our results may help in the endoscopic exploration of and microsurgical approaches to the fourth ventricle through its roof.

Shortening the learning curve in endoscopic endonasal skull base surgery: a reproducible polymer tumor model for the trans-sphenoidal trans-tubercular approach to retro-infundibular tumors.

BACKGROUND: Endoscopic endonasal skull base surgery attracts an increasing number of young neurosurgeons. This recent technique requires specific technical skills for the approaches to non-pituitary tumors (expanded endoscopic endonasal surgery). Actual residents' busy schedules carry the risk of compromising their laboratory training by limiting significantly the dedicated time for dissections. OBJECTIVE: To enhance and shorten the learning curve in expanded endoscopic endonasal skull base surgery, we propose a reproducible model based on the implantation of a polymer via an intracranial route to provide a pathological retro-infundibular expansive lesion accessible to a virgin expanded endoscopic endonasal route, avoiding the ethically-debatable need to hundreds of pituitary cases in live patients before acquiring the desired skills. METHODS: A polymer-based tumor model was implanted in 6 embalmed human heads via a microsurgical right fronto-temporal approach through the carotido-oculomotor cistern to mimic a retro-infundibular tumor. The tumor's position was verified by CT-scan. An endoscopic endonasal trans-sphenoidal trans-tubercular trans-planum approach was then carried out on a virgin route under neuronavigation tracking. RESULTS: Dissection of the tumor model from displaced surrounding neurovascular structures reproduced live surgery's sensations and challenges. Post-implantation CT-scan allowed the pre-removal assessment of the tumor insertion, its relationships as well as naso-sphenoidal anatomy in preparation of the endoscopic approach. CONCLUSION: Training on easily reproducible retro-infundibular approaches in a context of pathological distorted anatomy provides a unique opportunity to avoid the need for repetitive live surgeries to acquire skills for this kind of rare tumors, and may shorten the learning curve for endoscopic endonasal surgery.

Intradural endoscope-assisted anterior clinoidectomy: a cadaveric study.

OBJECTIVE: The anterior clinoid process (ACP) is critically related to the clinoidal portion of the internal carotid artery (ICA). The deep location of the ACP makes treatment of vascular and neoplastic lesions related to the ACP challenging. Removal of the ACP is advocated to facilitate treatment of such lesions. However injury to the clinoidal ICA remains a potential and dreadful complication of ACP removal. The aim of this study was to demonstrate an endoscopic assisted technique to perform intradural removal of the ACP via a pterional approach with continuous visualization of the clinoidal ICA. METHODS: Sixteen bilateral pterional dissections were performed in 8 glutaraldehyde embalmed, colored silicone injected, adult cadaveric heads. Using a standard pterional approach, we performed drilling of the ACP in 2 stages. Stage 1 consisted of extradural microscopic removal of the sphenoid ridge so as to gain access to the origin of the ACP. Stage 2, the endoscopic stage, consisted of intradural endoscopic removal of the ACP and mobilization of the clinoidal segment of the ICA. We used 2.7 mm, 0° and 30° angled endoscopes. RESULTS: In all the specimens we were able to remove the ACP while at the same time continuously visualizing the clinoidal ICA. The exposure of the clinoidal ICA and of adjoining neuro-vascular structures including the intracranial optic nerve was excellent and was accomplished with minimal frontal lobe retraction. Mobilization of the clinoidal ICA led to unhindered exposure of the parasellar region. CONCLUSIONS: Endoscopic assisted ACP removal with continuous ICA visualization was feasible in our model. Continuous visualization of the clinoidal ICA should theoretically decrease the risk of inadvertent ICA injuries. Clinical studies to validate this laboratory study are necessary.

Endoscope-assisted supraorbital approach to the retroinfundibular area: a cadaveric study.

The purpose of this study is to investigate and evaluate the exposure and maneuverability of this areas provided by an endoscope-assisted supraorbital approach and to compare that to a microscopic supraorbital approach. We exposed microscopically the optico-carotid and the infrachiasmatic windows after a supraorbital craniotomy executed using an eyebrow incision. We then proceeded to explore the retroinfundibular area using these two windows either using the microscope alone or using the endoscope-microscope combination where the microscope was used to (1) guide instrument and endoscope insertion into the surgical field, and (2) explore (with microscopic 3-d vision) subsegments of the endoscopic field of view. We compared the exposure and surgical maneuverability of the approach utilizing the microscopic mode alone with the endoscope-assisted mode. We evaluated the exposure and the surgical maneuverability of key anatomical structures of the retroinfundibular area. The structures evaluated included the diaphragma sellae, the dorsum sellae, the posterior clinoid process, the pituitary stalk, the mammillary bodies, the tuber cinereum, the oculomotor nerves, the basal pons, the upper trunk of the basilar artery, the superior cerebellar arteries, the posterior cerebral arteries, the posterior communicating arteries and the basilar bifurcation. The exposure and the surgical maneuverability were significantly higher in the endoscope-assisted mode (P < 0.0001). Based on our study, the endoscope-assisted supraorbital retroinfundibular approach is associated with larger exposure and maneuverability than the pure microscopic approach. Further clinical information is required to verify the results of this study.

Endoscope-assisted neurovascular decompression of the trigeminal nerve: a cadaveric study.

Endoscopes are increasingly used to examine cranial nerves in microvascular decompression (MVD) operations. The superior petrosal vein (SPV) is often purposely sacrificed to gain adequate exposure to the trigeminal nerve (TN) during MVD. Recently, the importance of preserving the SPV has been emphasized due to potential complications associated with its sacrifice. Our focus is to study the ability to operate on the TN with preservation of the SPV by using endoscope-assisted microsurgery. We studied both cerebellopontine angles in seven cadaveric heads which vascular system had been injected with colored silicon material. MVD procedures were simulated using the operative microscope (Moeller-Wedel, Cologne, Germany) and two fixed-angled (0°and 30°) rigid endoscopes (Aesculap, PA, USA). To compare the practical advantages of microscopic MVD (MMVD) and endoscope-assisted MVD (EAMVD), we divided the approaches into four subcategories (microscopic without and with SPV sacrifice and endoscope-assisted without and with SPV sacrifice) and compared the maneuverability associated with each category using a numerical grading system. EAMVD scored significantly better than MMVD both without and with cutting of the SPV (p < 0.001). Only in MMVD did cutting of the SPV improve the maneuverability especially in the superior quadrant of the nerve (p = 0.012). Based on the proposed scoring system, use of the endoscope in an assisted mode facilitates visualization and mobilization of the vascular loop associated with the TN without need to sacrifice the SPV. Sacrifice of the SVP may help maneuverability in the superior quadrant of the nerve in MMVD.

Path to the Interpeduncular Fossa: Anatomical Comparison of Endoscopic-Assisted versus Standard Subtemporal Approach.

Objective The aim of this study was to assess the assumed advantage of endoscopic assistance to the standard subtemporal approach. The idea was to measure qualitatively and quantitatively visibility versus operability. Design We performed eight subtemporal dissections on four cadaver heads. Our dissections integrated an operating microscope, endoscope, and neuronavigation. Comparison was made between visibility and operability afforded by the microscope alone or by the microscope-endoscope combination. Visibility was recorded as complete or incomplete and was quantified for key structures using linear measurements taken by the navigation system. Operability was determined by whichever maneuvers could be safely and comfortably accomplished in the space afforded. Results From our survey, the structures whose visibility most benefitted from the addition of the endoscope include: contralateral third nerve, posterior perforated substance, mammillary bodies, and contralateral superior cerebellar artery. With regard to quantitative evaluation, we found increased visibility of both basilar artery and posterior cerebral artery. With regard to the operability, no objective advantage was afforded by the addition of the endoscope. Subjectively, the maneuvers were easier to perform while using the endoscope. Conclusion Using the endoscope as an assistance tool during conducting classical subtemporal approach can help in overcome a lot of the classical subtemporal approach limitations.

Qualitative and quantitative radio-anatomical variation of the posterior clinoid process.

This study was conducted to investigate the radiological anatomy of the posterior clinoid process (PCP) to highlight preoperative awareness of its variations and its relationships to other skull base landmarks. The PCPs of 36, three-dimensional computed tomographic cadaveric heads were evaluated by studying the gross anatomy of the PCP and by measuring the distances between the PCP and other skull base anatomical landmarks relevant to transnasal or transcranial skull base approaches. PCP variations were found in five specimens (14%): in two the dorsum sellae was absent, in one the PCP and the anterior clinoid process (ACP) were connected unilaterally and in two bilaterally. The mean distance between the right/left PCP and the crista galli was 45.14 ± 4.0 standard deviation (SD_/46.24 ± 4.5 SD, respectively, while the distance to the middle point of the basion at the level of the foramen magnum was 40.41 ± 5.1 SD/41.0 ± 5.2 SD, respectively. The mean distance between the PCP and the ACP was 12.03 ± 3.18 SD on the right side and 12.11 ± 2.77 SD on the left. The data provided highlights the importance of careful preoperative evaluation of the PCP and of its relationships to other commonly encountered skull base landmarks. This information may give an idea of the exposure achievable through different transcranial and transnasal approaches. This is especially relevant when neuronavigation is not available.