ABSTRACT
This work describes the radiation characterisation and dosimetric measurements performed on the low-energy micromachining station of the femtosecond STELA (Santiago TErawatt LAser) at the University of Santiago de Compostela (Spain). For this aim, ionisation chambers, solid state detectors, and radiochromic films were used. The results show the emission of pulsed x-ray produced by laser-accelerated electrons from the ablated material exhibiting both bremsstrahlung and characteristic radiation. Although this radiation was produced unintentionally, a high superficial dose rate can be achieved. This radiation can be successfully stopped using small shielding to protect personnel from its effects. Based on the results of this work, the yearly dose equivalent after installing the shielding was negligible.
Subject(s)
Laser Therapy/methods , Radiometry/methodsABSTRACT
In this work a method for fabricating functionalized preclinical devices is presented. The manufacturing process combines a laser indirect writing technique to fabricate a soda-lime glass master and soft-lithography methods to obtain the final structure in polydimethylsiloxane (PDMS). The roughness of the device is modified in a controlled manner by applying a post-thermal treatment to the master, and thus devices with different roughness values are created. The PDMS devices are fully covered with human umbilical vein cells in a two-step process. In order to determine the most suitable device to perform bioassays, the cell attachment to the channel is evaluated with regards to the walls roughness when flow experiments are carried out.
Subject(s)
Biomedical Research , Lasers , Models, Cardiovascular , Tissue Engineering , Biocompatible Materials , Biomedical Research/instrumentation , Biomedical Research/methods , Calcium Compounds , Cells, Cultured , Equipment Design , Human Umbilical Vein Endothelial Cells , Humans , Oxides , Sodium Hydroxide , Tissue Engineering/instrumentation , Tissue Engineering/methodsABSTRACT
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