Exploring the antimicrobial efficacy of Manuka honey against multidrug-resistant and extensively drug-resistant Salmonella Typhi causing septicemia in Pakistan.

Aim: To determine the efficacy of manuka honey against multidrug-resistant (MDR) and extensively drug-resistant (XDR) clinical strains of Salmonella Typhi. Materials & methods: Clinical isolates were processed using the Bactec blood culture system, identification and antibiogram by Vitek 2 and antibiotic resistance genes through polymerase chain reaction (PCR). Microbroth dilution assays evaluated the antibacterial activity of manuka honey. Results: MDR and XDR-S. Typhi was susceptible to azithromycin. These strains carried the H58, gyrA, gyrB, blaCTX-M-15 , and blaTEM-1 genes. At 100% honey, the zone of inhibition for MDR (15-23 mm) and XDR (15-24 mm) strains. 18/50 MDR and 14/50 XDR strains inhibited at 3.125 v/v% killed at 6.25 v/v% concentration respectively. Conclusion: Manuka honey could be an alternative option for treating S. Typhi infections.

Typhoid fever is a life-threatening bacterial infection caused by the Salmonella Typhi. These bacteria are transmitted through contaminated water and food and cause fever, abdominal pain, headache, vomiting, and diarrhea mainly in children under 5. There are around 9 million people get infected with S. Typhi, with an increased death of 1,10,000 annually. Bees that collect nectar from the blossoms of the Manuka tree in Australia and New Zealand produce a type of honey known as manuka honey. This honey is famous for its antibacterial activity, and potential health benefits. Therefore, we aimed to determine its antibacterial activity against S. Typhi. Our finding shows that the commonly available antibiotics did not kill S. Typhi because their DNA was drug-resistant. After applying the manuka honey, these bacteria were killed and given a clear zone ranging from 15­24mm on the agar plate. Further analysis revealed that at low concentrations of manuka honey, 3.1% and 6.25%, most of the S. Typhi stopped growing and killed, respectively. This study suggested that manuka honey, which is affordable and readily available, could be used as a treatment option to treat infections produced by these harmful bacteria after further analysis.

Insights into chemistry, extraction and industrial application of lemon grass essential oil -A review of recent advances.

Lemongrass essential oil (LEO), extracted from high-oil lemongrass, gains prominence as a versatile natural product due to growing demand for safe health solutions. LEO comprises beneficial compounds like citral, isoneral, geraniol, and citronellal, offering diverse pharmacological benefits such as antioxidant, antifungal, antibacterial, antiviral, and anticancer effects. LEO finds applications in food preservation, cosmetics, and pharmaceuticals, enhancing profitability across these sectors. The review focuses on the extraction of LEO, emphasizing the need for cost-effective methods. Ultrasound and supercritical fluid extraction are effective in reducing extraction time, increasing yields, and enhancing oil quality. LEO shows promise as a valuable natural resource across industries, with applications in packaging, coating, and film development. LEO's ability to extend the shelf life of food items and impart natural flavors positions it as a valuable asset. Overall, the review emphasizes LEO's therapeutic, antimicrobial, and antioxidant properties, strengthening its potential in the food, pharmaceutical, and cosmetic sectors.

Uncovering the secret weapons of an invasive plant: The endophytic microbes of Anthemis cotula.

Understanding plant-microbe interaction can be useful in identifying the microbial drivers of plant invasions. It is in this context that we explored the diversity of endophytic microbes from leaves of Anthemis cotula, an annual plant that is highly invasive in Kashmir Himalaya. We also tried to establish the role of endophytes in the invasiveness of this alien species. We collected and processed leaf samples from three populations at three different sites. A total of 902 endophytic isolates belonging to 4 bacterial and 2 fungal phyla were recovered that belonged to 27 bacterial and 14 fungal genera. Firmicutes (29.1%), Proteobacteria (24.1%), Ascomycota (22.8%) and Actinobacteria (19%) were dominant across all samples. Plant growth promoting traits, such as Ammonia production, Indole Acetic Acid (IAA) production, Phosphate solubilization and biocontrol activity of these endophytes were also studied and most of the isolates (74.68%) were positive for ammonia production. IAA production, phosphate solubilization and biocontrol activity was present in 39.24%, 36.70% and 20.26% isolates, respectively. Furthermore, Botrytis cinerea, a pathogen of A. cotula in its native range, though present in Kashmir Himalaya does not affect A. cotula probably due to the presence of leaf endophytic microbial antagonists. Our results highlight that the beneficial plant growth promoting interactions and enemy suppression by leaf endophytes of A. cotula, may be contributing to its survival and invasion in the Kashmir Himalaya.

A glimpse into plant-based fermented products alternative to animal based products: Formulation, processing, health benefits.

Fermented foods and beverages are increasingly being included in the diets of people around the world, as they significantly contribute to flavor and interest in nutrition and food consumption. Plant sources, like cereals and pulses, are employed to produce vegan fermented foods that are either commercially available or the subject of ongoing scientific investigation. In addition, the inclination towards nutritionally healthy, natural, and clean-label products amongst consumers has encouraged the development of vegan fermented products alternative to animal-based products for industrial-scale production. However, as the vegan diet is more restrictive than the vegetarian diet, manufacturing food products for vegans presents a significant problem due to the limited availability of many raw materials. So further research is required on this topic. This paper aims to review the formulation, quality, microbial resources, health benefits, and safety of foods that can be categorised as vegan fermented foods and beverages.

Probiotic-fortified fruit juices: Health benefits, challenges, and future perspective.

Consumers' growing interest in using foods that improve health has motivated researchers and the food industry to develop new functional products, such as foods containing probiotics or live microbes. Probiotics have functional attributes that could satisfy most basic nutritional and therapeutic supplementation requirements. These microbes positively respond to clinical therapies against diseases and illnesses such as rotavirus-associated diarrhea, irritable bowel syndrome, and food allergies. Moreover, the role of probiotics in the prevention and treatment of obesity, diabetes, cancer, and diseases related to pathogenic microbes is an exciting and rapidly advancing research arena. Probiotic supplementation usually involves dairy products. However, because of the growing number of individuals affected by lactose intolerance and/or vegans, other food matrices like fruits, vegetables, cereals, and so on, have been studied as potential carriers for these microorganisms, presenting an alternative and better source in the process of assessing novel probiotic strains. The present review discusses the various factors affecting the survival of probiotics during storage in fruit juices, the possible effect of probiotics on sensory attributes and the overall acceptance of the products, and future technologies to improve the viability of probiotics.

Advances of nanofluid in food processing: Preparation, thermophysical properties, and applications.

Nanofluids (NFs) are homogenous mixes of solid nanoparticles as well as base fluid in which the size of the solid nanoparticles (NPs) is smaller than 100 nm. These solid NPs are intended to enhance the thermophysical characteristics and heat transmission attributes of the base fluid. The thermophysical characteristics of nanofluids are influenced by their density, viscosity, thermal conductivity and specific heat. These colloidal solutions of nanofluids include condensed nanomaterials such as nanoparticles, nanotubes, nanofibers, nanowires, nanosheets, and nanorods. The effectiveness of NF is significantly influenced by temperature, shape, size, type, as well as the concentration of NPs or the thermal characteristics of the base fluid. Compared to oxide NPs, metal NPs have superior thermal conductivity. Many of these investigations revealed that hybrid NFs had enhanced thermal conductivity than traditional ones. Thermal conductivity values are reduced by the formation of clusters in nanofluid. When compared to spherically formed nanoparticles, cylindrically shaped nanoparticles produced superior outcomes. In food industries, NFs could be used in various unit operations where heat needs to be transported from a heating or cooling medium to food product using a heat exchanger, as in freezing, pasteurization, refrigeration, drying, thawing, sterilization, and evaporation. The objective of this review is to analyze the recent developments in the research of nanofluids including innovative production methods, stability assessment, enhancement approaches, and thermophysical properties of nanofluids.

Insights into the seed microbiome and its ecological significance in plant life.

In recent years, the microbiome has attracted much attention because of the multiple roles and functions that microbes play in plants, animals, and human beings. Seed-associated microbes are of particular interest in being the initial microbial inoculum that affects the critical early life stages of a plant. The seed-microbe interactions are also known to improve nutrient acquisition, resilience against pathogens, and resistance against abiotic stresses. Despite these diverse roles, the seed microbiome has received little attention in plant ecology. Thus, we review the current knowledge on seed microbial diversity, community structure, and functions obtained through culture-dependent and culture-independent approaches. Furthermore, we present a comprehensive synthesis of the ecological literature on seed-microbe interactions to better understand the impact of these interactions on plant health and productivity. We suggest that future research should focus on the role of the seed microbiome in the establishment, colonization and spread of plant species in their native and non-native ranges as it may provide new insights into conservation biology and invasion ecology.

Promising bioactive properties of quercetin for potential food applications and health benefits: A review.

Naturally occurring phytochemicals with promising biological properties are quercetin and its derivatives. Quercetin has been thoroughly studied for its antidiabetic, antibacterial, anti-inflammatory, anti-Alzheimer's, anti-arthritic, antioxidant, cardiovascular, and wound-healing properties. Anticancer activity of quercetin against cancer cell lines has also recently been revealed. The majority of the Western diet contains quercetin and its derivatives, therefore consuming them as part of a meal or as a food supplement may be sufficient for people to take advantage of their preventive effects. Bioavailability-based drug-delivery systems of quercetin have been heavily studied. Fruits, seeds, vegetables, bracken fern, coffee, tea, and other plants all contain quercetin, as do natural colors. One naturally occurring antioxidant is quercetin, whose anticancer effects have been discussed in detail. It has several properties that could make it an effective anti-cancer agent. Numerous researches have shown that quercetin plays a substantial part in the suppression of cancer cells in the breast, colon, prostate, ovary, endometrial, and lung tumors. The current study includes a concise explanation of quercetin's action mechanism and potential health applications.

Phyllosphere microbiome: Diversity and functions.

Phyllosphere or aerial surface of plants represents the globally largest and peculiar microbial habitat that inhabits diverse and rich communities of bacteria, fungi, viruses, cyanobacteria, actinobacteria, nematodes, and protozoans. These hyperdiverse microbial communities are related to the host's specific functional traits and influence the host's physiology and the ecosystem's functioning. In the last few years, significant advances have been made in unravelling several aspects of phyllosphere microbiology, including diversity and microbial community composition, dynamics, and functional interactions. This review highlights the current knowledge about the assembly, structure, and composition of phyllosphere microbial communities across spatio-temporal scales, besides functional significance of different microbial communities to the plant host and the surrounding environment. The knowledge will help develop strategies for modelling and manipulating these highly beneficial microbial consortia for furthering scientific inquiry into their interactions with the host plants and also for their useful and economic utilization.

Evaluation of student’s perception and behavior towards plagiarism in Pakistani universities / Evaluación de la percepción y el comportamiento de estudiantes hacia el plagio en las universidades paquistaníes / Avaliação da percepção e do comportamento de estudantes para o plágio nas universidades paquistanesas

Background: The prevalence of academic delinquency in universities, predominantly in the form of plagiarism and cheating, is extensively conceded. Objectives: The objective of this study was to evaluate student’s perception and behavior towards plagiarism in Pakistani universities. Methods: This cross-sectional survey was conducted in 6 academic disciplines in 35 universities in Pakistan during 21.02.2011-21.04.2011. Expert interviewers and learned educationists carried out the interviews with the help of a structured questionnaire to evaluate student’s knowledge about plagiarism such as (i) student’s knowledge about HEC policy, (ii) student’s attitude towards plagiarism, (iii) student’s satisfaction towards plagiarism and cheating, and (iv) their views for penalties (What should be the penalties for 1st and 2nd occurrence of plagiarism?) and recorded the responses. Results: This survey was accomplished by 25742 students. The response rate for awareness about HEC policy about plagiarism showed that significantly (p<0.05) low percentage (94 percent of students was unaware of this policy. The attitudes of significantly (p<0.05) high percentage of students towards plagiarism behavior were unacceptable. Significantly (p<0.05) high response rate towards attitudes regarding punishment for plagiarism was warning and report to HOD for first and second occurrence of this cheating, respectively. Conclusions: An extensive lack was found in the understanding of students towards plagiarism across all the universities. It is necessary to achieve balance between factors affecting plagiarism such avoidance, finding and sentence in case of detection.

Antecedentes: La prevalencia de conductas éticas reprobables en las universidades, principalmente en la forma de plagio y engaño, es extensamente reconocida. Objetivos: El objetivo de este estudio fue evaluar la percepción y el comportamiento de los estudiantes hacia el plagio en las universidades paquistaníes. Métodos: Este estudio transversal se llevó a cabo en seis disciplinas académicas en 35 universidades en Pakistán durante 21.02.2011-21.04.2011. Entrevistadores expertos y educadores eruditos llevaron a cabo las entrevistas con la ayuda de un cuestionario estructurado, para evaluar los conocimientos de los estudiantes sobre el plagio: en aspectos como (i) el conocimiento de los estudiantes acerca de la política HEC, (ii) la actitud del estudiante: hacia el plagio, (iii) la satisfacción del estudiante: hacia el plagio y el engaño , y (iv) Sus puntos de vista en materia de sanciones (Qué sanciones debería otorgarse para la primera y segunda ocurrencia de plagio?) se grabaron las respuestas. Resultados: Esta encuesta fue realizado por 25.742 estudiantes. La tasa de respuesta para la conciencia acerca de las políticas HEC sobre el plagio mostraron significativamente (p <0,05) bajo porcentaje (94 por ciento). Los estudiantes no estaban al tanto de esta política. La actitud de rechazo al comportamiento de plagio muestra significativamente (p <0,05) un alto porcentaje. Los estudiantes consideraron que el plagio eran una conducta inaceptable. Significativamente (p <0,05) de alto índice fue la respuesta respecto a qué castigo se debería otorgar por plagio, la respuesta fue: advertencia e informar a HOD para la primera y segunda aparición de este engaño, respectivamente. Conclusiones: Se encontró una extensa falta de comprensión de los estudiantes hacia el plagio en todas las universidades. Es necesario para alcanzar el equilibrio entre los factores que influencian el plagio, lograr detectar la evasión, buscando y sentenciando los casos encontrados.

Antecedentes: A prevalência de condutas éticas reprováveis nas universidades, principalmente sob a forma de plágio e mentira, é extensamente reconhecida. Objetivos: Avaliar a percepção e o comportamento dos estudantes para o plágio nas universidades paquistanesas. Métodos: Este estudo transversal foi levado a cabo em seis disciplinas acadêmicas em 35 universidades no Paquistão de 21-02-2011 até 21-04-2011. Entrevistadores especialistas e educadores eruditos realizaram as entrevistas com a ajuda de um questionário estruturado, para avaliar os conhecimentos dos estudantes sobre o plágio: em aspectos como (i) o conhecimento dos estudantes acerca da política HEC, (ii) a atitude do estudante com relação ao plágio, (iii) a satisfação do estudante para com o plágio e a mentira, e (iv) seus pontos de vista em matéria de sanções (que sanções deveriam ser aplicadas para a primeira e segunda ocorrência de plágio?). Foram gravadas as respostas. Resultados: Esta enquete foi respondida por 25.742 estudantes. A taxa de resposta para a consciência acerca das políticas HEC sobre o plágio mostraram significativa (p<0,05) baixa porcentagem (94 por cento). Os estudantes não estavam a par desta política. A atitude de recusa ao comportamento do plágio mostra significativamente (p<0,05) uma alta porcentagem. Os estudantes consideraram que o plágio era uma conduta inaceitável. Significativamente (p<0,05) de alto índice foi a resposta a respeito do castigo que deveria ser dado ao plágio; a resposta foi: advertência e informar a HOD para o primeiro e segundo aparecimento deste engano, respectivamente. Conclusões: Foi encontrada uma extensa falta de compreensão dos estudantes em relação ao plágio em todas as universidades. É necessário, para alcançar o equilibrio entre os fatores que influenciam o plágio lograr detectar a evasão, buscando e sentenciando os casos encontrados.