Relative validity of a food frequency questionnaire for adolescents from a capital in the Northeastern region of Brazil

The present study was conducted to evaluate the validity of the Food Frequency Questionnaire (FFQ) used in the RPS Birth Cohort Consortium (Ribeirão Preto, Pelotas, and São Luís) to assess dietary intake of adolescents from São Luís, Maranhão. The research was developed with 152 adolescents aged 18 and 19 years. For the validation of the FFQ, the average of three 24-hour recalls (24HRs) was used as the reference method. The mean and standard deviation of energy and nutrient intake extracted from the surveys were estimated. The paired Student's t-test was used to verify the differences between the instruments. Pearson correlation coefficient, intraclass correlation coefficient (ICC), weighted Kappa, and the Bland-Altman plot were calculated in order to measure the agreement. The study adopted a level of significance <5%. Compared with the three 24HRs, the FFQ overestimated the consumption of most nutrients. Energy-adjusted and de-attenuated concordance Pearson correlation coefficients ranged from 0.06 to 0.43, and correlations were significant for iron, calcium, riboflavin, sodium, saturated fat, niacin, and vitamin C. The energy-adjusted and de-attenuated ICCs ranged from 0.01 to 0.31, and the weighted Kappa ranged from 0.01 to 0.46. The analyses of agreement were significant for vitamin C, fiber, calcium, riboflavin, niacin, sodium, lipids, and iron. In conclusion, the FFQ presented acceptable relative validity for lipids, saturated fatty acids, fiber, calcium, iron, riboflavin, niacin, vitamin C, and sodium. This instrument will be useful in studies about food consumption of adolescents in São Luís, Maranhão.

Relative validity of a food frequency questionnaire for adolescents from a capital in the Northeastern region of Brazil.

The present study was conducted to evaluate the validity of the Food Frequency Questionnaire (FFQ) used in the RPS Birth Cohort Consortium (Ribeirão Preto, Pelotas, and São Luís) to assess dietary intake of adolescents from São Luís, Maranhão. The research was developed with 152 adolescents aged 18 and 19 years. For the validation of the FFQ, the average of three 24-hour recalls (24HRs) was used as the reference method. The mean and standard deviation of energy and nutrient intake extracted from the surveys were estimated. The paired Student's t-test was used to verify the differences between the instruments. Pearson correlation coefficient, intraclass correlation coefficient (ICC), weighted Kappa, and the Bland-Altman plot were calculated in order to measure the agreement. The study adopted a level of significance <5%. Compared with the three 24HRs, the FFQ overestimated the consumption of most nutrients. Energy-adjusted and de-attenuated concordance Pearson correlation coefficients ranged from 0.06 to 0.43, and correlations were significant for iron, calcium, riboflavin, sodium, saturated fat, niacin, and vitamin C. The energy-adjusted and de-attenuated ICCs ranged from 0.01 to 0.31, and the weighted Kappa ranged from 0.01 to 0.46. The analyses of agreement were significant for vitamin C, fiber, calcium, riboflavin, niacin, sodium, lipids, and iron. In conclusion, the FFQ presented acceptable relative validity for lipids, saturated fatty acids, fiber, calcium, iron, riboflavin, niacin, vitamin C, and sodium. This instrument will be useful in studies about food consumption of adolescents in São Luís, Maranhão.

Reproductive ecology of phorid parasitoids in relation to the head size of leaf-cutting ants Atta sexdens Forel.

The leaf-cutting ant Atta sexdens Forel (Hymenoptera: Formicidae) is one of the most damaging agricultural pests in the Neotropics. Management strategies predominantly rely on the use of general insecticides. What is needed are more species-specific and environmentally friendly options. Parasitioids such as phorid flies (Diptera: Phoridae) may be one such option, but a greater understanding of the ecology of the flies and their ant hosts is essential to devise biological control strategies. Here we report parasitism rates, ant host size, parasitoid abundance per host and resultant sex ratios of two phorid species Apocephalus attophilus Borgmeier and Eibesfeldtphora tonhascai Brown parasitizing A.sexdens. The two species achieved parasitism rates of 1.48 and 1.46%, respectively and the pupal period was 14.7 ± 1.1 days and 22.1 ± 2.8 days, respectively. There was no significant difference between the head capsule width of ants parasitized by either A. attophilus or E. tonhascai. Likewise, there was no significant effect between the head capsule width of parasitized and unparasitized ants for both species. A significant positive correlation was found between the head capsule width of the parasitized ants and the number of adult parasitoids A. attophilus emerged. Ants parasitized by E. tonhascai survived significantly longer than those parasitized by A. attophilus. There was no significant effect of ant head width on the sex ratio of the offspring of either parasitoid species and no significant difference in the sex ratio (male: female) of their offspring. In summary, these data addressed here are important steps when considering natural enemies for biological control. Studying survival of the parasitized ants, parasitoid offspring sex ratio and host size preference allows for a better understanding of ant natural biological control in the field and can help in rearing of A. attophilus and E. tonhascai in laboratory.

The influence of espresso coffee on neurocognitive function in HIV-infected patients.

The aim of our study was to evaluate the impact of coffee intake on cognitive function in persons living with HIV (PLWH). 130 PLWH with CD4 > 200 cells/mm(3), undetectable viral load, treated with HAART were included. A structured interview was applied and relevant clinical and laboratory data were assessed, including coffee intake. For neuropsychological assessment, the HIV Neurobehavioral Research Center Battery was chosen. Univariate nonparametric statistics and multivariate regression model were used. A significant association between espresso coffee use and a better cognitive function was verified in five of the eight psychometric measurements. In the multivariate analysis, after variable adjustment, linear regression analysis showed that coffee intake was a positive predictor for attention/working memory, executive functions and Global Deficit Score. Although the mechanisms behind the influence of caffeine on cognitive functioning are controversial, regular espresso coffee intake may have favourable effects on cognitive deterioration caused by HIV.

The bipolarity of light and dark: A review on Bipolar Disorder and circadian cycles.

BACKGROUND: Bipolar Disorder is characterized by episodes running the full mood spectrum, from mania to depression. Between mood episodes, residual symptoms remain, as sleep alterations, circadian cycle disturbances, emotional deregulation, cognitive impairment and increased risk for comorbidities. The present review intends to reflect about the most recent and relevant information concerning the biunivocal relation between bipolar disorder and circadian cycles. METHODS: It was conducted a literature search on PubMed database using the search terms "bipolar", "circadian", "melatonin", "cortisol", "body temperature", "Clock gene", "Bmal1 gene", "Per gene", "Cry gene", "GSK3ß", "chronotype", "light therapy", "dark therapy", "sleep deprivation", "lithum" and "agomelatine". Search results were manually reviewed, and pertinent studies were selected for inclusion as appropriate. RESULTS: Several studies support the relationship between bipolar disorder and circadian cycles, discussing alterations in melatonin, body temperature and cortisol rhythms; disruption of sleep/wake cycle; variations of clock genes; and chronotype. Some therapeutics for bipolar disorder directed to the circadian cycles disturbances are also discussed, including lithium carbonate, agomelatine, light therapy, dark therapy, sleep deprivation and interpersonal and social rhythm therapy. LIMITATIONS: This review provides a summary of an extensive research for the relevant literature on this theme, not a patient-wise meta-analysis. CONCLUSIONS: In the future, it is essential to achieve a better understanding of the relation between bipolar disorder and the circadian system. It is required to establish new treatment protocols, combining psychotherapy, therapies targeting the circadian rhythms and the latest drugs, in order to reduce the risk of relapse and improve affective behaviour.

p63 and E-cadherin Expression in Canine Oral Squamous Cell Carcinoma.

The expression of p63 and E-cadherin was studied in 22 oral squamous cell carcinomas in the dog according to immunohistochemical techniques. The association between these markers and clinicopathologic parameters was assessed. All tumor cells studied showed enhanced p63 expression. Regarding E-cadherin expression, 17 of 22 cases (77.3%) showed decreased immunoreactivity, and in 13 of 22 cases (59.1%), its expression was cytoplasmic. Neither p63 nor E-cadherin expression patterns were associated with tumor size, bone invasion, or lymph node metastasis. p63 score was related to proliferating cell nuclear antigen proliferative index (P = .020). A statistically significant correlation between the expression patterns of these 2 markers was noted (P = .026). Furthermore, they were related with tumor grade. An atypical p63 labeling and a cytoplasmic E-cadherin staining were statistically related with a higher tumor grade (P = .022 and P = .017, respectively). These findings suggest that changes in p63 and E-cadherin expression are frequent events in oral squamous cell carcinoma in dogs.

Some like it cool: the effect of ambient temperature on the size of Anopheles funestus from southern Mozambique.

The wing lengths of 3,553 unfed, recently emerged, and 13,256 gravid female Anopheles funestus Giles from exit collections, undertaken between March 2004 and May 2005, were measured. Only in the warmest months were mean wing lengths of unfed females significantly smaller than gravid females. Mean wing lengths of unfed, females varied from 2.26 mm (Bootstrap 95% CI [2.25-2.27]) in January when mean air temperatures were 24.8 degrees C to 2.70 mm (Bootstrap 95% CI [2.68-2.72]) in July when mean temperatures were 8.4 degrees C cooler. Mean wing lengths increased by 0.05 mm for each degree decrease in air temperature. Temperature explained 60% of the variation in wing length.

The use of instrumental neutron activation and multivariate statistic analyses in differentiation of Brazilian phosphate ores.

Phosphated rocks from three ores located in the Middle-West region of Brazil can be used as low-level radioactive reference material due to the fact that they contain significant concentrations of thorium and, in some cases, of uranium. This paper describes the procedure to characterise those samples using several analytical methods. Statistical analysis and correlations studies have shown several geochemical similarities and the results obtained suggest the possibility to produce a reference material composed of a mixture of two similar ores.

Forager size of the leaf-cutting ant Atta sexdens (Hymenoptera: Formicidae) in a mature eucalyptus forest in Brazil.

We determined the size characteristics of foragers of the leaf-cutting ant Atta sexdens in a mature eucalyptus forest in Campos dos Goytacazes, Rio de Janeiro State, Brazil, at daytime (7:30 to 10:00 hr) and nighttime (19:00 to 23:00 hr). There were no significant differences between daytime and nighttime ant mass (Ma), but leaf fragment mass (Ml) and burden (B = [Ma + Ml]/Ma), which indicates relative load capacity, were significantly greater at daytime. There was a positive linear relationship between Ma and Ml for the combined daytime and nighttime data, and increases in Ma resulted in lower B. We compared A. sexdens characteristics with published results for Atta cephalotes, a closely related species. A. sexdens is larger and therefore able to carry heavier loads, but its burden is about 72% of the average value for A. cephalotes. We suggest that the lower load capacity of A. sexdens in comparison to A. cephalotes is related to its relatively larger size.

Effects of low feed intake and hot environment on plasma profiles of glucose, nonesterified fatty acids, insulin, glucagon, and IGF-I in lactating sows.

This experiment was designed to compare the effects of high ambient temperature and of feed restriction on plasma hormones and metabolites in primiparous lactating sows. Females were exposed to a constant thermoneutral (20 degrees C) or hot environment (30 degrees C) during lactation. Sows housed at 30 degrees C were given free access to feed (30AL: n = 12), whereas those housed at 20 degrees C were either pair-fed with those at 30 degrees C (20RF: n = 6) or were fed ad libitum (20AL: n = 6). A jugular vein catheter was surgically inserted in all sows at 100 d of gestation. Absorption of nutrients during the meal induced significant increases in plasma glucose, insulin, and glucagon, and a decrease in nonesterified fatty acids on Day 19 of lactation and Day 1 postweaning (P < 0.05). On Day 19, feed restriction at 20 degrees C was associated with higher plasma glucagon before the meal, lower plasma insulin after the meal and a lower insulin-to-glucagon ratio (I/GA) before and after the meal (P < 0.05). On Day 19, mean plasma concentrations measured in 30AL females were between those measured in 20AL and 20RF sows for nonesterified fatty acids and glucagon before feeding, and for glucose, nonesterified fatty acids, insulin, and glucagon after feeding. None of the differences between the 30AL and the 20RF groups was significant (P < 0.1). On Day 19, the only significant differences between the 30AL and 20AL groups were observed after the meal for plasma insulin and I/GA. Plasma insulin-like growth factor-I increased after farrowing in 20AL and 30AL sows only (P < 0.05). It was higher in 20AL than in 20RF and 30AL sows on Days 4 and 19 of lactation (P < 0.05). Overall, underfeeding at 20 degrees C induced changes in plasma insulin, glucagon, I/GA, and insulin-like growth factor-I, which would favor gluconeogenesis and body-reserve mobilization during lactation. Differences in glucagon and I/GA before the meal between well-fed sows at 20 degrees C and heat-exposed sows were attenuated, which could have detrimental consequences on glucose availability to the mammary gland and hence on milk production at 30 degrees C.

Acute outbreak of porcine parvovirus infection in Mozambique.

Investigations were made to determine the causal agent of an acute outbreak of abortions recorded in a swine herd in Mozambique. Isolation of porcine parvovirus and demonstration of its specific antibodies accomplished by using enzyme-linked immunosorbent assay, haemagglutination inhibition and immunofluorescent tests, indicated that porcine parvovirus was the causal agent of the abortions. Other pathogenic agents causing reproductive failure, e.g. pseudorabies virus, Leptospira or Brucella species, were ruled out because investigations of these agents proved to be negative.

Cryopreservation of boar semen in mini- and maxi-straws.

Split ejaculates from four boars were frozen with a programmable freezing machine, in mini- (0.25 ml) and maxi- (5 ml) plastic straws with an extender at either acidic (6.3) or alkaline (7.4) pH. Glycerol (3%) was used as cryoprotectant. The freezing of the semen was monitored by way of thermocouples placed in the straws. Post-thaw motility and acrosome integrity were evaluated; the latter using phase contrast microscopy, eosin-nigrosin stain and electron microscopy. Post-thaw sperm motility was significantly higher when semen was frozen in mini-straws than in maxi-straws. For the mini-straws, the motility was better when semen was exposed to an acidic environment during freezing, but this beneficial effect of the low extracellular pH was not evident when maxi-straws were thawed. The motility of the spermatozoa diminished significantly during the thermoresistance test (0 h and 2 h time) at 37 degrees C in a similar way for both straws and extracellular pH's. The freezing procedure, no matter the extracellular pH, did not cause major acrosomal damages, but significantly more normal apical ridges were present in the mini-straws than in the maxi-straws. This in vitro evaluation indicated that the freezing method employed was better for mini- than for maxi-straws since the freezing of the 5 ml volumes was not homogeneous, due to the large section area between the surface and the core of the straw.

Chromium in plants. Comparison between the concentration of chromium in Brazilian nonhypo and hypoglycemic plants.

Chromium is an essential trace element and is associated with some biological pathways, especially with glucose tolerance. For these reasons, we decided to determine the concentration of chromium in two sets of Brazilian medicinal plants. The first group consisted of plants that are considered as antidiabetic, whereas the second included plants that do not have this therapeutic property. The concentration of chromium was determined by flameless atomic absorption. All the plants analyzed contain chromium in the normal range for this element, but the hypoglycemic plants contain more chromium than the others (1-4 micrograms/g compared to 0.5-1.5 micrograms/g).