ABSTRACT
Qualitative rearrangement in erythropoiesis (changes in the ratio between erythrocyte populations and hemoglobin isoforms) was revealed in male Wistar rats with modeled acute aseptic inflammation. It was found that the percentage of heavy hemoglobin isoforms prevailed over the light forms in the bloodstream. Deciphering of the mechanisms of these changes requires additional research.
Subject(s)
Erythrocytes , Hemoglobins , Animals , Erythropoiesis , Inflammation , Male , Protein Isoforms/genetics , Rats , Rats, WistarABSTRACT
Six hemoglobin isoforms were identified in the peripheral blood of male Wistar rats by PAAG electrophoresis. Erythrocytes can be separated into 6 fractions by fractional centrifugation; the fractions differ by the content of reticulocytes and cells with fetal hemoglobin. Cells in each fraction contain one light and one heavy hemoglobin isoforms, and their combination is specific to each fraction. Changes in the hemoglobin isoforms in the whole blood in case of blood loss can be associated with changes in physical and chemical properties of circulating erythrocytes, as well as with the formation of various cell populations resulting from activated erythropoiesis.
Subject(s)
Anemia/blood , Hemoglobins/analysis , Hemorrhage/blood , Anemia/etiology , Animals , Disease Models, Animal , Erythrocyte Count , Erythrocytes/chemistry , Erythrocytes/metabolism , Hemoglobins/metabolism , Hemorrhage/complications , Male , Protein Isoforms/analysis , Protein Isoforms/blood , Rats , Rats, WistarABSTRACT
A significant role of the stress response to many different diseases prompted a search for new specialized and non-specialized anti-stress agents. This study examines the effect of the compound L17 from the group of 5-phenyl substituted-6H-1,3,4-thiadiazine-2-amines, on the manifestations of the stress response. The authors used a standard model of immobilization stress, in which an animal was immobilized on its back for 6h a day. Parameters of the morphological and functional states of the organs studied were measured and biochemical and enzyme-immunoassays were carried out on the first and second days. This study reveals that the main mechanism by which the L17 compound mediates of its anti-stress was by activation of macrophages on the second day of the experiments and the inhibition of apoptosis in the thymus. The results enable us to suggest that the compound L17 does not improve resistance to stress; however, it does lower the reaction to stress.