1.
FEBS Open Bio
; 10(8): 1474-1481, 2020 08.
Article
in English
| MEDLINE
| ID: mdl-32654405
ABSTRACT
Clostridium botulinum neurotoxins (BoNTs) cause flaccid paralysis through inhibition of acetylcholine release from motor neurons; however, at tiny doses, this property is exploited for use as a therapeutic. Each member of the BoNT family of proteins consists of three distinct domains: a binding domain that targets neuronal cell membranes (HC ), a translocation domain (HN ) and a catalytic domain (LC). Here, we present high-resolution crystal structures of the binding domains of BoNT subtypes/A5 (HC /A5) and/A6 (HC /A6). These structures show that the core fold identified in other subtypes is maintained, but with subtle differences at the expected receptor-binding sites.
Subject(s)
Botulinum Toxins, Type A/chemistry , Botulinum Toxins, Type A/isolation & purification , Crystallography, X-Ray , Models, Molecular , Protein Conformation
2.
Infect Control Hosp Epidemiol
; 41(5): 592-593, 2020 05.
Article
in English
| MEDLINE
| ID: mdl-32122422