The potential of FT-IR spectroscopy for improving healthcare in sepsis - An animal model study.

Fourier Transform-Infrared (FT-IR) absorption spectroscopy has been used to investigate pathophysiological changes caused by sepsis. Sepsis has been defined as a potentially fatal organic dysfunction caused by a dysregulated host response to infection and can lead a patient to risk of death. This study used samples consisting of the blood plasma of mice which were induced to sepsis state, compared to a healthy group using FT-IR associated with attenuated total reflectance (ATR) spectroscopy. For statistical analysis, principal components analysis (PCA) and linear discriminant analysis (LDA) were applied, independently, to the second derivative spectra of both the fingerprint (900-1800 cm-1) and the high wavenumber (2800-3100 cm-1) regions. The technique efficiently differentiated the blood plasma of the two groups, sepsis and healthy mice, the analysis indicating that fatty acids and lipids in the blood samples could be an important biomarker of sepsis.

Short report - Herpes simplex lesion in the lip semimucosa in a COVID-19 patient.

OBJECTIVE: The present case report aims to make a discussion concerning oral manifestations in a patient with a confirmed diagnosis of COVID-19. Female patient, 20 years old, nursing technician, showed severe sore throat and headache without presence of fever. She tested positive for COVID-19 RT-PCR test in 2 episodes. She also showed lesions in the median lower lip semimucosa and severe pruritus, with a clinical course of 14 days, in which we performed a clinical diagnosis of herpes simplex infection. We need to be precise in terms of clinical appearance and possible relation with the disease, as the clinicians have access to the patients.

Vibration spectroscopy and body biofluids: Literature review for clinical applications.

Vibrational spectroscopy techniques such as Raman and IR (infrared) allow real-time, non-invasive and non-destructive analysis of organic compounds with a good limit-of-detection. This review aims to show the progress of clinical diagnosis and prognosis due to advances of vibrational spectroscopy techniques in biofluids through an extensive literature review. This review was performed by searching for studies using the keywords "biofluids or biological fluids" and "diagnostic techniques" in PubMed, Scopus and Google Scholar. We found 580 articles in the 1990s, 1171 articles in the 2000s and 1688 in the years from 2011. Also, a second search including "biofluids or biological fluids" and "vibrational spectroscopy" returned only one article in the 1990s, three papers in the 2000s and 18 in the years from 2011.This growth suggests a great potential of biofluid research using vibrational spectroscopy. Sample collection variations(quantity and contaminations due to contact with other body parts and their secretions) are important factors that influence sample composition. Once these factors are taken into account, spectroscopic analysis may provide the necessary information to identify a disease, lesion, tumor or infection. With the present review we aim to encourage the study of vibrational spectroscopy techniques for analysis of biofluids focusing in clinical applications. In the future, it will widely benefit clinicians, allowing new diagnostic approaches, and for patients to have early diagnosis for most every disease.