Detection of faecal bacteria and antibiotic resistance genes in biofilms attached to plastics from human-impacted coastal areas.

Plastics have been proposed as vectors of bacteria as they act as a substrate for biofilms. In this study, we evaluated the abundance of faecal and marine bacteria and antibiotic resistance genes (ARGs) from biofilms adhered to marine plastics. Floating plastics and plastics from sediments were collected in coastal areas impacted by human faecal pollution in the northwestern Mediterranean Sea. Culture and/or molecular methods were used to quantify faecal indicators (E. coli, Enterococci and crAssphage), and the ARGs sulI, tetW and blaTEM and the 16S rRNA were detected by qPCR assays. Pseudomonas and Vibrio species and heterotrophic marine bacteria were also analysed via culture-based methods. Results showed that, plastic particles covered by bacterial biofilms, primarily consisted of marine bacteria including Vibrio spp. Some floating plastics had a low concentration of viable E. coli and Enterococci (42% and 67% of the plastics respectively). Considering the median area of the plastics, we detected an average of 68 cfu E. coli per item, while a higher concentration of E. coli was detected on individual plastic items, when compared with 100 ml of the surrounding water. Using qPCR, we quantified higher values of faecal indicators which included inactive and dead microorganisms, detecting up to 2.6 × 102 gc mm-2. The ARGs were detected in 67-88% of the floating plastics and in 29-57% of the sediment plastics with a concentration of up to 6.7 × 102 gc mm-2. Furthermore, enrichment of these genes was observed in biofilms compared with the surrounding water. These results show that floating plastics act as a conduit for both the attachment and transport of faecal microorganisms. In contrast, low presence of faecal indicators was detected in plastic from seafloor sediments. Therefore, although in low concentrations, faecal bacteria, and potential pathogens, were identified in marine plastics, further suggesting plastics act as a reservoir of pathogens and ARGs.

Multiproxy characterization of sedimentary facies in a submarine sulphide mine tailings dumping site and their environmental significance: The study case of Portmán Bay (SE Spain).

Mining activities are essential to our society, but ore extraction and treatment produce waste that must be stored in safe places without harm to the environment. For a long time, seafloor disposal has been viewed as a cheap option with barely visible impacts. In Portmán Bay, SE of Spain, large amounts of tailings from open pit sulphide mining were discharged directly into the coastal sea over 33 years, thus forming a massive deposit that completely infilled the bay and expanded seawards over the inner continental shelf. Here we present the first multiproxy physicochemical characterization of the submarine tailings in Portmán Bay, mostly by using non-destructive techniques, also including pre-dumping and post-dumping sediments. Eight distinct sedimentary facies, grouped in four stratigraphic units, have been thus identified in a set of up to 4.3 m long gravity cores totalling more than 60 m. Geogenic and anthropogenic geochemical proxies consistently allow differentiating pre-dumping sediments from tailings. Potentially toxic metals if made bioavailable can reach high concentrations in units including or formed exclusively by tailings (i.e. up to 3455, 2755 and 1007 mg kg-1 for Pb, As, and Zn, respectively). Some physical properties, such as magnetic susceptibility, are particularly useful as the tailings are rich in Fe-bearing minerals (>30% Fe in some layers). Estimated sedimentation rates show a strong gradient from proximal to distal locations, with rates in excess of 50 cm yr-1 to less than 1 cm yr-1. We ultimately document the history of the transformation of Portmán Bay from an almost natural state to a new condition after a long period of massive dumping of mine tailings. Our study provides guidance to further assessments in a context where the diversity of marine environments impacted by the disposal of mine waste is expected to grow in the near future.

Remobilization of dissolved metals from a coastal mine tailing deposit driven by groundwater discharge and porewater exchange.

Mining impacts on coastal environments have been extensively studied around the world. However, the role of Submarine Groundwater Discharge (SGD) and Porewater Exchange (PEX) as pathways for pollutants from mining waste deposits into seawater has been largely overlooked. Portmán Bay is located in the Cartagena-La Unión Pb-Zn sulphur mining district in Murcia, SE of Spain. The disposal of about 60 million tons of metal-rich mine tailings from 1957 to 1990 led to the infill of most of the bay. Although the effects of metals on indicator organisms have been shown previously, there is a major lack of knowledge on the release of dissolved metals from the emerged tailing deposit into the sea, more than 25 years after the closure of the mining activities. Samples for Ra isotopes (223Ra, 224Ra, 226Ra and 228Ra) and dissolved metals (Ag, Cd, Co, Pb, Zn) were analyzed in porewaters and seawater in order to separately estimate SGD and PEX driven dissolved metal fluxes. Our results show a continuous release of dissolved metals into the sea driven by both PEX and SGD. Most of dissolved metals are remobilized and released into the water column by PEX, which is a ubiquitous mechanism acting along the shoreline. Although SGD only represents 13% of the water flow, it drives large fluxes of dissolved Fe into the sea, mainly restricted to the west side of the bay. Large inputs of dissolved Fe2+ from the anoxic tailings deposit trigger a massive precipitation of iron hydroxides that enables the removal of most dissolved metals from the water column. This study highlights the role of PEX and SGD as significant mechanisms for the land to ocean transfer of dissolved metals from coastal mine tailings deposits.

Constraining the temporal variations of Ra isotopes and Rn in the groundwater end-member: Implications for derived SGD estimates.

Submarine groundwater discharge (SGD) has been recognized as an important supplier of chemical compounds to the ocean that may influence coastal geochemical cycles. Radium isotopes (223Ra, 224Ra, 226Ra,228Ra) and radon (222Rn) have been widely applied as tracers of SGD. Their application requires the appropriate characterization of both the concentrations of tracers in the discharging groundwater and their distribution in the coastal water column. This study evaluates the temporal evolution of Ra isotopes and 222Rn concentrations in a dynamic subterranean estuary of a microtidal Mediterranean coastal aquifer that experiences large displacements of the fresh-saltwater interface as a necessary initial step in evaluating the influence of SGD in coastal waters. We show that changes in groundwater salinities due to the seaward displacement of the fresh-saltwater interface produced large variations in Ra activities in groundwater (by a factor of ~19, ~14, ~6, and ~11 for 223Ra, 224Ra, 226Ra and 228Ra, respectively), most importantly during rainfall events. In contrast, the 222Rn activities in groundwater oscillated only by a factor of 3 during these rainy periods. The large temporal variability in Ra activities hampers the characterization of the SGD end-member when using Ra isotopes as tracers, and thus presents a challenge for obtaining accurate SGD estimates. This study emphasizes the need to understand the hydrodynamics of coastal aquifers to appropriately constrain the Ra isotopes and 222Rn concentrations in groundwater and when applying both tracers in dynamic microtidal coastal systems.