ABSTRACT
The development of human endometrial carcinoma (HEC) is a complex pathologic process involves several oncogenes and tumor suppressor genes. The full-length paired-box gene 2 (pax2), a recently discovered oncogene, promotes cell proliferation and growth and inhibits apoptosis of HEC cells. Here, we examined the effect of pax2 small interfering RNA (siRNA) on the growth of transplanted HEC cells in nude mice. The expression of Pax2 in 21 cases of normal endometrium and 38 cases of HEC was examined by immohistochemistry (IHC). HEC models were developed by subcutaneously transferring HEC cells into nude mice, followed by treatment with empty lentivirus vector, lentivirus vector-based pax2 siRNA, and phosphate buffered saline, respectively. Four weeks later, tumor size was measured, tumor inhibition rate was calculated, and histological analyses were conducted after staining with hematoxylin and eosin. The expression of Pax2 and Bcl-2 was detected by Western blot; proliferating cell nuclear antigen (PCNA) was detected by IHC. Significant differences were observed in the positive rate of Pax2 between normal endometrium and HEC (14.2% vs. 60.5%, P < 0.01). The expression index of Pax2 in well differentiated tumors was 1.88 ± 1.68, much lower than that in tumors of moderate (3.07 ± 1.96, P < 0.05) or poor differentiation (5.45 ± 2.76, P <0.01). Tumor necrosis increased, nuclear basophilia stain decreased, tumor growth was inhibited, and PCNA, Pax2, and Bcl-2 expression was reduced in HEC models treated with pax2 siRNA. These results indicate that Pax2 expression is related to HEC tumor biology with the increased expression of Pax2 correlated to malignancy. pax2 siRNA down-regulates Pax2 expression and inhibits tumorigenesis of HEC in nude mice, possibly due to cell apoptosis and the inhibition of tumor proliferation induced by down-regulation of Bcl-2.
Subject(s)
Adult , Aged , Animals , Female , Humans , Mice , Middle Aged , Apoptosis , Cell Line, Tumor , Cell Proliferation , Down-Regulation , Endometrial Neoplasms , Metabolism , Pathology , Genetic Vectors , Lentivirus , Genetics , Mice, Inbred BALB C , Mice, Nude , Neoplasm Transplantation , PAX2 Transcription Factor , Genetics , Metabolism , Proliferating Cell Nuclear Antigen , Metabolism , Proto-Oncogene Proteins c-bcl-2 , Metabolism , RNA Interference , RNA, Small Interfering , Genetics , Transfection , Tumor BurdenABSTRACT
UNLABELLED: The aim of this study was to explore the molecular pathological characteristics of cervical cancer in women aged below 35, disclose the factors that indicate the unfavorable prognosis, and provide some useful targets for improving therapeutic outcome. PATIENTS AND METHODS: 64 cases of pathological data originating from cervical cancer patients aged below 35 served as the study group to perform a retrospective research, and pathological data from 90 cases of cervical cancer patients beyond age 35, who underwent treatment during the same time, were selected at random as the reference group. Immunohistochemistry and quantified image analysis were utilized to detect synchronously the protein expression difference of survivin and p27 between the 2 groups. RESULTS: The total 5-year survival rate of the study group was 65.6% compared to 84.4% in the reference group, while the lymph node metastasis rate was 25% compared to 12.2% in the reference group. Both differences were statistically significant (p < 0.05). Survivin expression is much greater in the study group than in the reference group (p < 0.05); there is no statistical difference of the p27 expression between the 2 groups (p > 0.05), but p27 expression is related to lymph node metastasis. CONCLUSION: Younger female patients have a worse prognosis than older ones, and survivin expression is associated with poor prognosis. Targeting these molecular factors may lead to a hopeful biomedical therapy to improve the prognosis of young female cervical cancer patients.
Subject(s)
Biomarkers, Tumor/analysis , Microtubule-Associated Proteins/analysis , Neoplasm Proteins/analysis , Proliferating Cell Nuclear Antigen/analysis , Risk Assessment/methods , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/epidemiology , Adolescent , Adult , Child , China/epidemiology , Female , Humans , Incidence , Inhibitor of Apoptosis Proteins , Prognosis , Reproducibility of Results , Risk Factors , Sensitivity and Specificity , Survival Analysis , Survival Rate , Survivin , Uterine Cervical Neoplasms/metabolismABSTRACT
@#ObjectiveTo investigate the diagnoses of somatic diseases of inpatients with psychosis and hospitalized more then 2 years.MethodsThe material of all 350 inpatients were investigated and analyzed.ResultsThe first three psychiatric diagnoses were schizophrenia (61.32%), Alzheimer's disease (15.26%), mood disorder (4.74%). The first three diagnoses of accompanying somatic diseases were heart disease (8.68%), diabetes mellitus (8.42%), and hypertension (4.47%).ConclusionWith the changes of social environment, the disease pedigree of inpatients with psychosis changes, senile patients become more and more, and the incidence of concurrent somatic diseases gradually increases.